(Circular) (Six-base) MAPSORT of: pFN21AE6389.seq Check: 8654 from: 1 to: 5860 pFN21AE6389 5860 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3670 278 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2658 3151 3203 1937 Size: 721 493 52 4594 Fragments arranged by size: 4594 721 493 52 Acc65I G'GTAC_C Cuts at: 3134 3181 3134 Size: 47 5813 AclI AA'CG_TT Cuts at: 1563 3987 4360 1563 Size: 2424 373 3063 Fragments arranged by size: 3063 2424 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2063 2370 3923 5307 5575 1632 Size: 431 307 1553 1384 268 1917 Fragments arranged by size: 1917 1553 1384 431 307 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5663 AflIII A'CryG_T Cuts at: 1203 4759 5736 1203 Size: 3556 977 1327 Fragments arranged by size: 3556 1327 977 AgeI A'CCGG_T Cuts at: 4691 4691 Size: 5860 AhdI GACnn_n'nnGTC Cuts at: 4589 4589 Size: 5860 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5860 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5860 AlwNI CAG_nnn'CTG Cuts at: 2348 3603 5175 2348 Size: 1255 1572 3033 Fragments arranged by size: 3033 1572 1255 ApaI G_GGCC'C Cuts at: 2480 2480 Size: 5860 ApaLI G'TGCA_C Cuts at: 3917 5073 3917 Size: 1156 4704 ApoI r'AATT_y Cuts at: 1493 3169 3395 3547 5656 1493 Size: 1676 226 152 2109 1697 Fragments arranged by size: 2109 1697 1676 226 152 AseI AT'TA_AT Cuts at: 160 1032 4414 160 Size: 872 3382 1606 Fragments arranged by size: 3382 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 AvaI C'yCGr_G Cuts at: 1870 1943 2415 3185 1870 Size: 73 472 770 4545 Fragments arranged by size: 4545 770 472 73 BamHI G'GATC_C Cuts at: 3190 3557 3190 Size: 367 5493 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2128 3059 3134 3181 4636 Size: 325 217 627 341 931 75 47 1455 Cuts at: 4636 618 Size: 1842 Fragments arranged by size: 1842 1455 931 627 341 325 217 75 47 BanII G_rGCy'C Cuts at: 1391 2480 2737 2967 3179 1391 Size: 1089 257 230 212 4072 Fragments arranged by size: 4072 1089 257 230 212 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4889 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4051 4085 1903 Size: 34 50 34 2030 34 3678 Fragments arranged by size: 3678 2030 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3752 4962 3752 Size: 1210 4650 BclI T'GATC_A Cuts at: 1548 1548 Size: 5860 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2801 4471 136 Size: 107 122 71 631 605 1129 1670 1525 Fragments arranged by size: 1670 1525 1129 631 605 122 107 71 BglII A'GATC_T Cuts at: 5855 5855 Size: 5860 BlpI GC'TnA_GC Cuts at: 3277 3277 Size: 5860 Bme1580I G_kGCm'C Cuts at: 1790 2142 2480 3921 5077 1790 Size: 352 338 1441 1156 2573 Fragments arranged by size: 2573 1441 1156 352 338 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5860 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3591 4549 442 Size: 416 53 83 399 24 2174 958 1753 Fragments arranged by size: 2174 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5860 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2325 2807 2981 4520 1239 Size: 1086 482 174 1539 2579 Fragments arranged by size: 2579 1539 1086 482 174 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3985 4850 5148 3985 Size: 865 298 4697 Fragments arranged by size: 4697 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4523 882 Size: 617 3024 2219 Fragments arranged by size: 3024 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3651 493 Size: 711 2447 2702 Fragments arranged by size: 2702 2447 711 BsaBI GATnn'nnATC Cuts at: 5854 5854 Size: 5860 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3667 4049 4684 275 Size: 53 83 186 936 2134 382 635 1451 Fragments arranged by size: 2134 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2010 4293 4691 4965 5112 5523 2010 Size: 2283 398 274 147 411 2347 Fragments arranged by size: 2347 2283 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5635 Fragments arranged by size: 5635 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5860 BseYI C'CCAG_C Cuts at: 2031 3016 5063 5559 2031 Size: 985 2047 496 2332 Fragments arranged by size: 2332 2047 985 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2171 2760 1163 Size: 573 435 589 4263 Fragments arranged by size: 4263 589 573 435 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3310 4071 5099 1540 Size: 275 186 1309 761 1028 2301 Fragments arranged by size: 2301 1309 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2737 2821 3002 3921 4006 5077 2737 Size: 84 181 919 85 1071 3520 Fragments arranged by size: 3520 1071 919 181 85 84 BsmI GAATG_Cn' Cuts at: 2649 3371 3464 2649 Size: 722 93 5045 Fragments arranged by size: 5045 722 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2480 2737 2821 2967 3002 3179 Size: 399 352 338 257 84 146 35 177 Cuts at: 3179 3921 4006 5077 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 352 338 257 177 146 85 84 35 BspEI T'CCGG_A Cuts at: 2010 5523 2010 Size: 3513 2347 BspHI T'CATG_A Cuts at: 3749 5479 3749 Size: 1730 4130 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2624 3215 844 Size: 1780 591 3489 Fragments arranged by size: 3489 1780 591 BsrBI CCG'CTC Cuts at: 1394 1823 3747 1394 Size: 429 1924 3507 Fragments arranged by size: 3507 1924 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4355 4529 65 Size: 1183 3107 174 1396 Fragments arranged by size: 3107 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4504 4691 1700 Size: 110 2694 187 2869 Fragments arranged by size: 2869 2694 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5860 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5860 BssSI C'ACGA_G Cuts at: 3920 4932 3920 Size: 1012 4848 BstAPI GCAn_nnn'nTGC Cuts at: 794 2071 794 Size: 1277 4583 BstBI TT'CG_AA Cuts at: 4664 4664 Size: 5860 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5860 BstXI CCAn_nnnn'nTGG Cuts at: 3091 3091 Size: 5860 BstYI r'GATC_y Cuts at: 1972 2612 3190 3557 3942 3959 5411 5497 5509 Size: 640 578 367 385 17 1452 86 12 Cuts at: 5509 5855 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 640 578 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2271 2688 513 Size: 552 1206 417 3685 Fragments arranged by size: 3685 1206 552 417 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5121 Fragments arranged by size: 5121 683 56 BtsI GCAGTG_nn' Cuts at: 2319 2969 3376 4170 4190 2319 Size: 650 407 794 20 3989 Fragments arranged by size: 3989 794 650 407 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5860 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5825 DraI TTT'AAA Cuts at: 3164 3164 Size: 5860 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4867 809 Size: 507 3551 1802 Fragments arranged by size: 3551 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2194 2649 2952 Size: 54 1425 50 275 18 364 455 303 Cuts at: 2952 3081 3307 4196 5825 8 Size: 129 226 889 1629 43 Fragments arranged by size: 1629 1425 889 455 364 303 275 226 129 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3307 1537 Size: 275 1495 4090 Fragments arranged by size: 4090 1495 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2185 2400 3790 1353 Size: 832 215 1390 3423 Fragments arranged by size: 3423 1390 832 215 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4447 4821 4967 387 Size: 1064 138 2858 374 146 1280 Fragments arranged by size: 2858 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2735 2735 Size: 5860 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2325 2370 2807 2981 3923 4520 Size: 393 431 262 45 437 174 942 597 Cuts at: 4520 5307 5575 1239 Size: 787 268 1524 Fragments arranged by size: 1524 942 787 597 437 431 393 268 262 174 45 EcoO109I rG'GnC_Cy Cuts at: 2477 2568 2707 2477 Size: 91 139 5630 Fragments arranged by size: 5630 139 91 EcoRI G'AATT_C Cuts at: 3169 3547 3169 Size: 378 5482 EcoRV GAT'ATC Cuts at: 2501 2501 Size: 5860 FspI TGC'GCA Cuts at: 4366 4366 Size: 5860 HaeII r_GCGC'y Cuts at: 1678 5007 1678 Size: 3329 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2485 2490 2517 2522 2560 Size: 32 135 32 913 5 27 5 38 Cuts at: 2560 2592 4501 4533 4575 4607 1373 Size: 32 1909 32 42 32 2626 Fragments arranged by size: 2626 1909 913 135 42 38 32 32 32 32 32 27 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 2176 2464 3204 3450 669 Size: 988 281 238 288 740 246 3079 Fragments arranged by size: 3079 988 740 288 281 246 238 HindIII A'AGCT_T Cuts at: 748 748 Size: 5860 HpaI GTT'AAC Cuts at: 3450 3450 Size: 5860 KpnI G_GTAC'C Cuts at: 3138 3185 3138 Size: 47 5813 MfeI C'AATT_G Cuts at: 2133 3459 2133 Size: 1326 4534 MluI A'CGCG_T Cuts at: 5736 5736 Size: 5860 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4974 5158 1305 Size: 3669 184 2007 Fragments arranged by size: 3669 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2651 2954 3083 10 Size: 54 1425 343 819 303 129 2787 Fragments arranged by size: 2787 1425 819 343 303 129 54 MslI CAynn'nnrTG Cuts at: 518 1166 2549 2912 2948 2981 3818 4177 4336 Size: 648 1383 363 36 33 837 359 159 Cuts at: 4336 518 Size: 2042 Fragments arranged by size: 2042 1383 837 648 363 359 159 36 33 MspA1I CmG'CkG Cuts at: 1606 2035 2068 3016 3276 3953 5101 5346 1606 Size: 429 33 948 260 677 1148 245 2120 Fragments arranged by size: 2120 1148 948 677 429 260 245 33 NcoI C'CATG_G Cuts at: 513 1065 2688 513 Size: 552 1623 3685 Fragments arranged by size: 3685 1623 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5860 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5860 NotI GC'GGCC_GC Cuts at: 3307 3307 Size: 5860 NspI r_CATG'y Cuts at: 1132 1229 2113 2219 3218 4763 1132 Size: 97 884 106 999 1545 2229 Fragments arranged by size: 2229 1545 999 884 106 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5860 PciI A'CATG_T Cuts at: 4759 4759 Size: 5860 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5860 PfoI T'CCnGG_A Cuts at: 2100 2958 2100 Size: 858 5002 PmeI GTTT'AAAC Cuts at: 3164 3164 Size: 5860 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3929 3961 5442 5474 3929 Size: 32 1481 32 4315 Fragments arranged by size: 4315 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2568 2568 Size: 5860 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5860 PsiI TTA'TAA Cuts at: 3430 3430 Size: 5860 PspOMI G'GGCC_C Cuts at: 2476 2476 Size: 5860 PstI C_TGCA'G Cuts at: 830 2923 3212 830 Size: 2093 289 3478 Fragments arranged by size: 3478 2093 289 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5860 PvuII CAG'CTG Cuts at: 2068 3016 2068 Size: 948 4912 SacI G_AGCT'C Cuts at: 2737 2737 Size: 5860 SalI G'TCGA_C Cuts at: 1936 3202 1936 Size: 1266 4594 SbfI CC_TGCA'GG Cuts at: 3212 3212 Size: 5860 ScaI AGT'ACT Cuts at: 4108 5820 4108 Size: 1712 4148 SfcI C'TryA_G Cuts at: 826 1045 2837 2919 3208 4343 5024 5215 826 Size: 219 1792 82 289 1135 681 191 1471 Fragments arranged by size: 1792 1471 1135 681 289 219 191 82 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5860 SmaI CCC'GGG Cuts at: 1872 2417 3187 1872 Size: 545 770 4545 Fragments arranged by size: 4545 770 545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3964 4865 5127 820 Size: 197 926 2021 901 262 1553 Fragments arranged by size: 2021 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3651 493 Size: 3158 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5860 SphI G_CATG'C Cuts at: 1132 3218 1132 Size: 2086 3774 SspI AAT'ATT Cuts at: 5 52 3784 5 Size: 47 3732 2081 Fragments arranged by size: 3732 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5860 StyI C'CwwG_G Cuts at: 513 1065 2688 2715 2868 3299 513 Size: 552 1623 27 153 431 3074 Fragments arranged by size: 3074 1623 552 431 153 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3887 3904 4057 4242 4687 325 Size: 1255 2307 17 153 185 445 1498 Fragments arranged by size: 2307 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4106 5818 96 Size: 274 80 33 51 1443 2129 1712 138 Fragments arranged by size: 2129 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5820 5852 5820 Size: 32 5828 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5860 XbaI T'CTAG_A Cuts at: 3196 3196 Size: 5860 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2947 3013 2947 Size: 66 5794 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5860 XmaI C'CCGG_G Cuts at: 1870 2415 3185 1870 Size: 545 770 4545 Fragments arranged by size: 4545 770 545 XmnI GAAnn'nnTTC Cuts at: 3989 3989 Size: 5860 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3668 276 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI