################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BalI BamHI BbeI BbrPI BcgI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRV EcoT22I FseI HgaI Hin1I HindIII HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I RcaI RsrII SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SspI SwaI XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Aor51HI 2186 AGCGCT AGC^GCT AspI 1479 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 456 GGTGCC G^G[CT][GA]CC BbvCI 1202 CCTCAGC CCTCAGC(-5/-2) BclI 372 TGATCA T^GATCA BglI 2267 GCCGGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsiEI 1719 CGATCG CG[GA][CT]^CG Bsp1407I 1389 TGTACA T^GTACA BsrGI 1389 TGTACA T^GTACA BstAPI 2373 GCAACCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1275 AGATCC [GA]^GATC[CT] DraII 182 GGGACCC [GA]G^G[ACGT]CC[CT] DrdI 602 GACTTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 323 TGGCCG [CT]^GGCC[GA] Eco47III 2186 AGCGCT AGC^GCT EcoO109I 182 GGGACCC [GA]G^G[ACGT]CC[CT] FauI 2392 CCCGC CCCGC(4/6) FbaI 372 TGATCA T^GATCA HaeII 2186 AGCGCT [GA]GCGC^[CT] MaeII 2258 ACGT A^CGT MfeI 1238 CAATTG C^AATTG MflI 1275 AGATCC [GA]^GATC[CT] MslI 1542 CACAAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 1238 CAATTG C^AATTG PaeR7I 1193 CTCGAG C^TCGAG PflFI 1479 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 182 GGGACCC [GA]G^G[AT]CC[CT] PshAI 2050 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1054 CTGCAG CTGCA^G PvuI 1719 CGATCG CGAT^CG SacI 228 GAGCTC GAGCT^C SapI 589 GAAGAGC GCTCTTC(1/4) SbfI 1053 CCTGCAGG CCTGCA^GG Sse8387I 1053 CCTGCAGG CCTGCA^GG SspBI 1389 TGTACA T^GTACA Tth111I 1479 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1410 CCAGCCTGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1193 CTCGAG C^TCGAG XhoII 1275 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AlwI 560 GATCC GGATC(4/5) 1276 GATCC AvaII 183 GGACC G^G[AT]CC 1255 GGACC AviII 284 TGCGCA TGC^GCA 1292 TGCGCA BanII 228 GAGCTC G[GA]GC[CT]^C 1305 GAGCCC BbsI 676 GAAGAC GAAGAC(2/6) 1770 GTCTTC BciVI 261 GGATAC GTATCC(6/5) 1662 GGATAC BpuAI 676 GAAGAC GAAGAC(2/6) 1770 GTCTTC BsaI 901 GAGACC GGTCTC(1/5) 1083 GAGACC BsrDI 311 GCAATG GCAATG(2/0) 2164 GCAATG BsrFI 269 ACCGGC [GA]^CCGG[CT] 2267 GCCGGT Cfr10I 269 ACCGGC [GA]^CCGG[CT] 2267 GCCGGT EcoNI 351 CCTTGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 447 CCTTGATAAGG EcoRI 867 GAATTC G^AATTC 1509 GAATTC FspI 284 TGCGCA TGC^GCA 1292 TGCGCA HapII 270 CCGG C^CGG 2268 CCGG HpaII 270 CCGG C^CGG 2268 CCGG MspI 270 CCGG C^CGG 2268 CCGG PflMI 1624 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2206 CCAGGCTCTGG PleI 1726 GACTC GAGTC(4/5) 2055 GAGTC Van91I 1624 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2206 CCAGGCTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AciI AcsI AfaI AluI AlwNI ApoI AspHI AvaI BbvI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I