(Circular) (Six-base) MAPSORT of: pFN21AE5761.seq Check: 1856 from: 1 to: 7138 pFN21AE5761 7138 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4948 278 Size: 53 83 186 936 3412 2468 Fragments arranged by size: 3412 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4481 1937 Size: 2544 4594 Acc65I G'GTAC_C Cuts at: 4459 4459 Size: 7138 AclI AA'CG_TT Cuts at: 1563 5265 5638 1563 Size: 3702 373 3063 Fragments arranged by size: 3702 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2078 2208 2273 2861 2960 5201 6585 6853 Size: 446 130 65 588 99 2241 1384 268 Cuts at: 6853 1632 Size: 1917 Fragments arranged by size: 2241 1917 1384 588 446 268 130 99 65 AfeI AGC'GCT Cuts at: 4193 4193 Size: 7138 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6941 AflIII A'CryG_T Cuts at: 1203 6037 7014 1203 Size: 4834 977 1327 Fragments arranged by size: 4834 1327 977 AgeI A'CCGG_T Cuts at: 5969 5969 Size: 7138 AhdI GACnn_n'nnGTC Cuts at: 5867 5867 Size: 7138 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7138 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7138 AlwNI CAG_nnn'CTG Cuts at: 2513 3635 4217 4235 4881 6453 2513 Size: 1122 582 18 646 1572 3198 Fragments arranged by size: 3198 1572 1122 646 582 18 ApaLI G'TGCA_C Cuts at: 5195 6351 5195 Size: 1156 5982 ApoI r'AATT_y Cuts at: 1493 2872 3514 3748 4447 4673 4825 6934 1493 Size: 1379 642 234 699 226 152 2109 1697 Fragments arranged by size: 2109 1697 1379 699 642 234 226 152 AseI AT'TA_AT Cuts at: 160 1032 5692 160 Size: 872 4660 1606 Fragments arranged by size: 4660 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7138 AvaI C'yCGr_G Cuts at: 1870 1943 2545 2650 3198 4463 1870 Size: 73 602 105 548 1265 4545 Fragments arranged by size: 4545 1265 602 548 105 73 BamHI G'GATC_C Cuts at: 4468 4835 4468 Size: 367 6771 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2461 4459 5914 618 Size: 325 217 627 674 1998 1455 1842 Fragments arranged by size: 1998 1842 1455 674 627 325 217 BanII G_rGCy'C Cuts at: 1391 2237 3314 4457 1391 Size: 846 1077 1143 4072 Fragments arranged by size: 4072 1143 1077 846 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2688 3768 928 Size: 971 789 1080 4298 Fragments arranged by size: 4298 1080 971 789 BbvCI CC'TCA_GC Cuts at: 3208 3208 Size: 7138 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5329 5363 1903 Size: 34 3392 34 3678 Fragments arranged by size: 3678 3392 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2260 3661 5030 6240 2260 Size: 1401 1369 1210 3158 Fragments arranged by size: 3158 1401 1369 1210 BclI T'GATC_A Cuts at: 1548 2377 1548 Size: 829 6309 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4278 5749 136 Size: 107 122 71 631 605 2606 1471 1525 Fragments arranged by size: 2606 1525 1471 631 605 122 107 71 BglII A'GATC_T Cuts at: 7133 7133 Size: 7138 BlpI GC'TnA_GC Cuts at: 4555 4555 Size: 7138 Bme1580I G_kGCm'C Cuts at: 1790 4269 5199 6355 1790 Size: 2479 930 1156 2573 Fragments arranged by size: 2573 2479 1156 930 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7138 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3161 3711 3855 Size: 416 53 83 399 24 1744 550 144 Cuts at: 3855 3869 4869 5827 442 Size: 14 1000 958 1753 Fragments arranged by size: 1753 1744 1000 958 550 416 399 144 83 53 24 14 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7138 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2639 3362 4194 4325 5798 1239 Size: 1400 723 832 131 1473 2579 Fragments arranged by size: 2579 1473 1400 832 723 131 Bpu10I CC'TnA_GC Cuts at: 2409 3208 2409 Size: 799 6339 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2932 4078 5263 6128 6426 2932 Size: 1146 1185 865 298 3644 Fragments arranged by size: 3644 1185 1146 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2900 3082 5801 882 Size: 617 1401 182 2719 2219 Fragments arranged by size: 2719 2219 1401 617 182 BsaAI yAC'GTr Cuts at: 493 1204 4929 493 Size: 711 3725 2702 Fragments arranged by size: 3725 2702 711 BsaBI GATnn'nnATC Cuts at: 7132 7132 Size: 7138 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4945 5327 5962 275 Size: 53 83 186 936 3412 382 635 1451 Fragments arranged by size: 3412 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5571 5969 6243 6390 6801 5571 Size: 398 274 147 411 5908 Fragments arranged by size: 5908 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3879 3909 4280 4310 1178 Size: 30 165 30 2476 30 371 30 4006 Fragments arranged by size: 4006 2476 371 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2337 2837 3162 3638 4339 1186 Size: 1151 500 325 476 701 3985 Fragments arranged by size: 3985 1151 701 500 476 325 BseYI C'CCAG_C Cuts at: 2218 3324 4268 4299 4316 6341 6837 2218 Size: 1106 944 31 17 2025 496 2519 Fragments arranged by size: 2519 2025 1106 944 496 31 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6565 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3727 4588 5349 6377 1540 Size: 275 186 1726 861 761 1028 2301 Fragments arranged by size: 2301 1726 1028 861 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2237 2984 3166 5199 5284 6355 2237 Size: 747 182 2033 85 1071 3020 Fragments arranged by size: 3020 2033 1071 747 182 85 BsmI GAATG_Cn' Cuts at: 4649 4742 4649 Size: 93 7045 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2237 2984 3166 3314 4269 4457 5199 Size: 399 447 747 182 148 955 188 742 Cuts at: 5199 5284 6355 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 955 747 742 447 399 188 182 148 85 BspEI T'CCGG_A Cuts at: 6801 6801 Size: 7138 BspHI T'CATG_A Cuts at: 5027 6757 5027 Size: 1730 5408 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2603 2825 3100 4123 4493 844 Size: 1759 222 275 1023 370 3489 Fragments arranged by size: 3489 1759 1023 370 275 222 BsrBI CCG'CTC Cuts at: 1394 1823 5025 1394 Size: 429 3202 3507 Fragments arranged by size: 3507 3202 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2323 4176 5633 5807 65 Size: 1183 1075 1853 1457 174 1396 Fragments arranged by size: 1853 1457 1396 1183 1075 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2274 4272 5782 5969 1700 Size: 110 464 1998 1510 187 2869 Fragments arranged by size: 2869 1998 1510 464 187 110 BsrGI T'GTAC_A Cuts at: 96 3394 96 Size: 3298 3840 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7138 BssSI C'ACGA_G Cuts at: 5198 6210 5198 Size: 1012 6126 BstAPI GCAn_nnn'nTGC Cuts at: 794 4384 794 Size: 3590 3548 BstBI TT'CG_AA Cuts at: 5942 5942 Size: 7138 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7138 BstYI r'GATC_y Cuts at: 1972 3280 4468 4835 5220 5237 6689 6775 6787 Size: 1308 1188 367 385 17 1452 86 12 Cuts at: 6787 7133 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1308 1188 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5647 Fragments arranged by size: 5647 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2072 4295 487 Size: 683 56 846 2223 3330 Fragments arranged by size: 3330 2223 846 683 56 BtsI GCAGTG_nn' Cuts at: 4654 5448 5468 4654 Size: 794 20 6324 Fragments arranged by size: 6324 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7138 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7103 DraI TTT'AAA Cuts at: 4442 4442 Size: 7138 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2613 6145 809 Size: 507 1297 3532 1802 Fragments arranged by size: 3532 1802 1297 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2328 4585 5474 Size: 54 1425 50 275 18 498 2257 889 Cuts at: 5474 7103 8 Size: 1629 43 Fragments arranged by size: 2257 1629 1425 889 498 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4585 1537 Size: 275 2773 4090 Fragments arranged by size: 4090 2773 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2328 2589 2754 2898 3526 3739 5068 1353 Size: 975 261 165 144 628 213 1329 3423 Fragments arranged by size: 3423 1329 975 628 261 213 165 144 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2245 3887 5725 6099 6245 387 Size: 1064 138 656 1642 1838 374 146 1280 Fragments arranged by size: 1838 1642 1280 1064 656 374 146 138 EcoICRI GAG'CTC Cuts at: 2235 2235 Size: 7138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2208 2273 2639 2861 2960 3362 Size: 393 446 130 65 366 222 99 402 Cuts at: 3362 4194 4325 5201 5798 6585 6853 1239 Size: 832 131 876 597 787 268 1524 Fragments arranged by size: 1524 876 832 787 597 446 402 393 366 268 222 131 130 99 65 EcoNI CCTnn'n_nnAGG Cuts at: 2360 2456 2360 Size: 96 7042 EcoO109I rG'GnC_Cy Cuts at: 2188 2188 Size: 7138 EcoRI G'AATT_C Cuts at: 2872 3514 4447 4825 2872 Size: 642 933 378 5185 Fragments arranged by size: 5185 933 642 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2798 3665 3935 2798 Size: 867 270 6001 Fragments arranged by size: 6001 867 270 FspI TGC'GCA Cuts at: 2291 3299 5644 2291 Size: 1008 2345 3785 Fragments arranged by size: 3785 2345 1008 HaeII r_GCGC'y Cuts at: 1678 4195 6285 1678 Size: 2517 2090 2531 Fragments arranged by size: 2531 2517 2090 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3617 3649 3716 3748 4240 Size: 32 135 32 2045 32 67 32 492 Cuts at: 4240 4272 5779 5811 5853 5885 1373 Size: 32 1507 32 42 32 2626 Fragments arranged by size: 2626 2045 1507 492 135 67 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2583 2857 3077 4482 4728 669 Size: 988 281 645 274 220 1405 246 3079 Fragments arranged by size: 3079 1405 988 645 281 274 246 220 HindIII A'AGCT_T Cuts at: 748 748 Size: 7138 HpaI GTT'AAC Cuts at: 4728 4728 Size: 7138 KpnI G_GTAC'C Cuts at: 4463 4463 Size: 7138 MfeI C'AATT_G Cuts at: 3243 4737 3243 Size: 1494 5644 MluI A'CGCG_T Cuts at: 7014 7014 Size: 7138 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2244 6252 6436 1305 Size: 939 4008 184 2007 Fragments arranged by size: 4008 2007 939 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5316 Fragments arranged by size: 5316 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3551 5096 5455 5614 518 Size: 648 2385 1545 359 159 2042 Fragments arranged by size: 2385 2042 1545 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3212 3340 4235 4303 4554 5231 6379 6624 Size: 1606 128 895 68 251 677 1148 245 Cuts at: 6624 1606 Size: 2120 Fragments arranged by size: 2120 1606 1148 895 677 251 245 128 68 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5647 Fragments arranged by size: 5647 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7138 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7138 NotI GC'GGCC_GC Cuts at: 4585 4585 Size: 7138 NspI r_CATG'y Cuts at: 1132 1229 2207 3298 3556 4294 4496 6041 1132 Size: 97 978 1091 258 738 202 1545 2229 Fragments arranged by size: 2229 1545 1091 978 738 258 202 97 PasI CC'CwG_GG Cuts at: 1348 3965 3991 1348 Size: 2617 26 4495 Fragments arranged by size: 4495 2617 26 PciI A'CATG_T Cuts at: 6037 6037 Size: 7138 PflMI CCAn_nnn'nTGG Cuts at: 1108 3635 4217 1108 Size: 2527 582 4029 Fragments arranged by size: 4029 2527 582 PfoI T'CCnGG_A Cuts at: 2638 4147 2638 Size: 1509 5629 PmeI GTTT'AAAC Cuts at: 4442 4442 Size: 7138 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5207 5239 6720 6752 5207 Size: 32 1481 32 5593 Fragments arranged by size: 5593 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2188 2188 Size: 7138 PshAI GACnn'nnGTC Cuts at: 1613 4059 1613 Size: 2446 4692 PsiI TTA'TAA Cuts at: 4708 4708 Size: 7138 PspXI vC'TCGA_Gb Cuts at: 3198 3198 Size: 7138 PstI C_TGCA'G Cuts at: 830 3063 4490 830 Size: 2233 1427 3478 Fragments arranged by size: 3478 2233 1427 PvuI CG_AT'CG Cuts at: 2001 3727 2001 Size: 1726 5412 PvuII CAG'CTG Cuts at: 3212 3340 4235 4303 3212 Size: 128 895 68 6047 Fragments arranged by size: 6047 895 128 68 SacI G_AGCT'C Cuts at: 2237 2237 Size: 7138 SalI G'TCGA_C Cuts at: 1936 4480 1936 Size: 2544 4594 SanDI GG'GwC_CC Cuts at: 2188 2188 Size: 7138 SapI GCTCTTCn'nnn_ Cuts at: 2589 2589 Size: 7138 SbfI CC_TGCA'GG Cuts at: 3063 4490 3063 Size: 1427 5711 ScaI AGT'ACT Cuts at: 5386 7098 5386 Size: 1712 5426 SfcI C'TryA_G Cuts at: 826 1045 2720 3059 4187 4373 4486 5621 6302 Size: 219 1675 339 1128 186 113 1135 681 Cuts at: 6302 6493 826 Size: 191 1471 Fragments arranged by size: 1675 1471 1135 1128 681 339 219 191 186 113 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7138 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7138 SmaI CCC'GGG Cuts at: 1872 4465 1872 Size: 2593 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2911 3198 4057 5242 6143 6405 Size: 197 926 968 287 859 1185 901 262 Cuts at: 6405 820 Size: 1553 Fragments arranged by size: 1553 1185 968 926 901 859 287 262 197 SnaBI TAC'GTA Cuts at: 493 4929 493 Size: 4436 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7138 SphI G_CATG'C Cuts at: 1132 2207 3298 3556 4496 1132 Size: 1075 1091 258 940 3774 Fragments arranged by size: 3774 1091 1075 940 258 SspI AAT'ATT Cuts at: 5 52 5062 5 Size: 47 5010 2081 Fragments arranged by size: 5010 2081 47 StuI AGG'CCT Cuts at: 1517 2146 3946 4281 1517 Size: 629 1800 335 4374 Fragments arranged by size: 4374 1800 629 335 StyI C'CwwG_G Cuts at: 513 1065 2004 2390 3289 3864 4577 513 Size: 552 939 386 899 575 713 3074 Fragments arranged by size: 3074 939 899 713 575 552 386 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2012 3874 3978 5165 5182 5335 5520 Size: 1255 432 1862 104 1187 17 153 185 Cuts at: 5520 5965 325 Size: 445 1498 Fragments arranged by size: 1862 1498 1255 1187 445 432 185 153 104 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3394 3997 5384 Size: 274 80 33 51 1443 1417 603 1387 Cuts at: 5384 7096 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1417 1387 603 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3879 3911 4280 4312 7098 7130 3879 Size: 32 369 32 2786 32 3887 Fragments arranged by size: 3887 2786 369 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3487 1316 Size: 2171 4967 XbaI T'CTAG_A Cuts at: 4474 4474 Size: 7138 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3422 3422 Size: 7138 XhoI C'TCGA_G Cuts at: 1943 3198 1943 Size: 1255 5883 XmaI C'CCGG_G Cuts at: 1870 4463 1870 Size: 2593 4545 XmnI GAAnn'nnTTC Cuts at: 5267 5267 Size: 7138 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4946 276 Size: 53 83 186 936 3412 2468 Fragments arranged by size: 3412 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI