(Linear) (Six-base) MAPSORT of: KIEE5761.seq Check: 3377 from: 1 to: 2433 KIEE5761.orf With 182 enzymes: SgfI * December 5, 2014 13:09 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 73 203 268 856 955 2433 Size: 73 130 65 588 99 1478 Fragments arranged by size: 1478 588 130 99 73 65 AfeI AGC'GCT Cuts at: 0 2188 2433 Size: 2188 245 AlwNI CAG_nnn'CTG Cuts at: 0 508 1630 2212 2230 2433 Size: 508 1122 582 18 203 Fragments arranged by size: 1122 582 508 203 18 ApoI r'AATT_y Cuts at: 0 867 1509 1743 2433 Size: 867 642 234 690 Fragments arranged by size: 867 690 642 234 AvaI C'yCGr_G Cuts at: 0 540 645 1193 2433 Size: 540 105 548 1240 Fragments arranged by size: 1240 548 540 105 BanI G'GyrC_C Cuts at: 0 456 2433 Size: 456 1977 BanII G_rGCy'C Cuts at: 0 232 1309 2433 Size: 232 1077 1124 Fragments arranged by size: 1124 1077 232 BbsI GAAGACnn'nnnn_ Cuts at: 0 683 1763 2433 Size: 683 1080 670 Fragments arranged by size: 1080 683 670 BbvCI CC'TCA_GC Cuts at: 0 1203 2433 Size: 1203 1230 BciVI GTATCCnnnnn_n' Cuts at: 0 255 1656 2433 Size: 255 1401 777 Fragments arranged by size: 1401 777 255 BclI T'GATC_A Cuts at: 0 372 2433 Size: 372 2061 BglI GCCn_nnn'nGGC Cuts at: 0 2273 2433 Size: 2273 160 Bme1580I G_kGCm'C Cuts at: 0 2264 2433 Size: 2264 169 BmrI ACTGGGnnnn_n' Cuts at: 0 1156 1706 1850 1864 2433 Size: 1156 550 144 14 569 Fragments arranged by size: 1156 569 550 144 14 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 634 1357 2189 2320 2433 Size: 634 723 832 131 113 Fragments arranged by size: 832 723 634 131 113 Bpu10I CC'TnA_GC Cuts at: 0 404 1203 2433 Size: 404 799 1230 Fragments arranged by size: 1230 799 404 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 927 2073 2433 Size: 927 1146 360 Fragments arranged by size: 1146 927 360 BsaI GGTCTCn'nnnn_ Cuts at: 0 895 1077 2433 Size: 895 182 1356 Fragments arranged by size: 1356 895 182 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1874 1904 2275 2305 2433 Size: 1874 30 371 30 128 Fragments arranged by size: 1874 371 128 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 332 832 1157 1633 2334 2433 Size: 332 500 325 476 701 99 Fragments arranged by size: 701 500 476 332 325 99 BseYI C'CCAG_C Cuts at: 0 213 1319 2263 2294 2311 2433 Size: 213 1106 944 31 17 122 Fragments arranged by size: 1106 944 213 122 31 17 BsiEI CG_ry'CG Cuts at: 0 1722 2433 Size: 1722 711 BsiHKAI G_wGCw'C Cuts at: 0 232 979 1161 2433 Size: 232 747 182 1272 Fragments arranged by size: 1272 747 232 182 Bsp1286I G_dGCh'C Cuts at: 0 232 979 1161 1309 2264 2433 Size: 232 747 182 148 955 169 Fragments arranged by size: 955 747 232 182 169 148 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 598 820 1095 2118 2433 Size: 598 222 275 1023 315 Fragments arranged by size: 1023 598 315 275 222 BsrDI GCAATG_nn' Cuts at: 0 318 2171 2433 Size: 318 1853 262 Fragments arranged by size: 1853 318 262 BsrFI r'CCGG_y Cuts at: 0 269 2267 2433 Size: 269 1998 166 Fragments arranged by size: 1998 269 166 BsrGI T'GTAC_A Cuts at: 0 1389 2433 Size: 1389 1044 BstAPI GCAn_nnn'nTGC Cuts at: 0 2379 2433 Size: 2379 54 BstYI r'GATC_y Cuts at: 0 1275 2433 Size: 1275 1158 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 67 2290 2433 Size: 67 2223 143 Fragments arranged by size: 2223 143 67 DrdI GACnn_nn'nnGTC Cuts at: 0 608 2433 Size: 608 1825 EaeI y'GGCC_r Cuts at: 0 323 2433 Size: 323 2110 EarI CTCTTCn'nnn_ Cuts at: 0 323 584 749 893 1521 1734 2433 Size: 323 261 165 144 628 213 699 Fragments arranged by size: 699 628 323 261 213 165 144 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 240 1882 2433 Size: 240 1642 551 Fragments arranged by size: 1642 551 240 EcoICRI GAG'CTC Cuts at: 0 230 2433 Size: 230 2203 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 73 203 268 634 856 955 1357 2189 Size: 73 130 65 366 222 99 402 832 Cuts at: 2189 2320 2433 Size: 131 113 Fragments arranged by size: 832 402 366 222 131 130 113 99 73 65 EcoNI CCTnn'n_nnAGG Cuts at: 0 355 451 2433 Size: 355 96 1982 Fragments arranged by size: 1982 355 96 EcoO109I rG'GnC_Cy Cuts at: 0 183 2433 Size: 183 2250 EcoRI G'AATT_C Cuts at: 0 867 1509 2433 Size: 867 642 924 Fragments arranged by size: 924 867 642 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 793 1660 1930 2433 Size: 793 867 270 503 Fragments arranged by size: 867 793 503 270 FspI TGC'GCA Cuts at: 0 286 1294 2433 Size: 286 1008 1139 Fragments arranged by size: 1139 1008 286 HaeII r_GCGC'y Cuts at: 0 2190 2433 Size: 2190 243 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1612 1644 1711 1743 2235 2267 2433 Size: 1612 32 67 32 492 32 166 Fragments arranged by size: 1612 492 166 67 32 32 32 HincII GTy'rAC Cuts at: 0 578 852 1072 2433 Size: 578 274 220 1361 Fragments arranged by size: 1361 578 274 220 MfeI C'AATT_G Cuts at: 0 1238 2433 Size: 1238 1195 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 239 2433 Size: 239 2194 MslI CAynn'nnrTG Cuts at: 0 1546 2433 Size: 1546 887 MspA1I CmG'CkG Cuts at: 0 1207 1335 2230 2298 2433 Size: 1207 128 895 68 135 Fragments arranged by size: 1207 895 135 128 68 NspI r_CATG'y Cuts at: 0 202 1293 1551 2289 2433 Size: 202 1091 258 738 144 Fragments arranged by size: 1091 738 258 202 144 PasI CC'CwG_GG Cuts at: 0 1960 1986 2433 Size: 1960 26 447 Fragments arranged by size: 1960 447 26 PflMI CCAn_nnn'nTGG Cuts at: 0 1630 2212 2433 Size: 1630 582 221 Fragments arranged by size: 1630 582 221 PfoI T'CCnGG_A Cuts at: 0 633 2142 2433 Size: 633 1509 291 Fragments arranged by size: 1509 633 291 PpuMI rG'GwC_Cy Cuts at: 0 183 2433 Size: 183 2250 PshAI GACnn'nnGTC Cuts at: 0 2054 2433 Size: 2054 379 PspXI vC'TCGA_Gb Cuts at: 0 1193 2433 Size: 1193 1240 PstI C_TGCA'G Cuts at: 0 1058 2433 Size: 1058 1375 PvuI CG_AT'CG Cuts at: 0 1722 2433 Size: 1722 711 PvuII CAG'CTG Cuts at: 0 1207 1335 2230 2298 2433 Size: 1207 128 895 68 135 Fragments arranged by size: 1207 895 135 128 68 SacI G_AGCT'C Cuts at: 0 232 2433 Size: 232 2201 SanDI GG'GwC_CC Cuts at: 0 183 2433 Size: 183 2250 SapI GCTCTTCn'nnn_ Cuts at: 0 584 2433 Size: 584 1849 SbfI CC_TGCA'GG Cuts at: 0 1058 2433 Size: 1058 1375 SfcI C'TryA_G Cuts at: 0 715 1054 2182 2368 2433 Size: 715 339 1128 186 65 Fragments arranged by size: 1128 715 339 186 65 SmlI C'TyrA_G Cuts at: 0 906 1193 2052 2433 Size: 906 287 859 381 Fragments arranged by size: 906 859 381 287 SphI G_CATG'C Cuts at: 0 202 1293 1551 2433 Size: 202 1091 258 882 Fragments arranged by size: 1091 882 258 202 StuI AGG'CCT Cuts at: 0 141 1941 2276 2433 Size: 141 1800 335 157 Fragments arranged by size: 1800 335 157 141 StyI C'CwwG_G Cuts at: 0 385 1284 1859 2433 Size: 385 899 575 574 Fragments arranged by size: 899 575 574 385 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 7 1869 1973 2433 Size: 7 1862 104 460 Fragments arranged by size: 1862 460 104 7 TatI w'GTAC_w Cuts at: 0 1389 1992 2433 Size: 1389 603 441 Fragments arranged by size: 1389 603 441 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1874 1906 2275 2307 2433 Size: 1874 32 369 32 126 Fragments arranged by size: 1874 369 126 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1482 2433 Size: 1482 951 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1417 2433 Size: 1417 1016 XhoI C'TCGA_G Cuts at: 0 1193 2433 Size: 1193 1240 Enzymes that do cut: AcuI AfeI AlwNI ApoI AvaI BanI BanII BbsI BbvCI BciVI BclI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstYI BtgZI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII MfeI MmeI MslI MspA1I NspI PasI PflMI PfoI PpuMI PshAI PspXI PstI PvuI PvuII SacI SanDI SapI SbfI SfcI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BfrBI BglII BlpI BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaWI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrBI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI DraIII EagI EcoRV FseI FspAI HindIII HpaI KasI KpnI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PsiI PspOMI PsrI RsrII SacII SalI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI XbaI XmaI XmnI ZraI