################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglII BlnI BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FseI FspI HaeII Hin1I HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI NarI NdeI NotI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccII 25 CGCG CG^CG AcsI 1031 GAATTC [GA]^AATT[CT] AflIII 1407 ACATGT A^C[GA][CT]GT ApaI 335 GGGCCC GGGCC^C ApoI 1031 GAATTC [GA]^AATT[CT] AvaII 370 GGACC G^G[AT]CC BalI 1226 TGGCCA TGG^CCA BclI 1499 TGATCA T^GATCA BlpI 304 GCTGAGC GC^T[ACGT]AGC Bpu1102I 304 GCTGAGC GC^T[ACGT]AGC BsaBI 1010 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 1339 GTGCAG GTGCAG(16/14) BsmBI 427 CGTCTC CGTCTC(1/5) BsmI 242 GCATTC GAATGC(1/-1) BspLU11I 1407 ACATGT A^CATGT BsrBI 331 GAGCGG CCGCTC(-3/-3) BsrDI 1389 GCAATG GCAATG(2/0) BstUI 25 CGCG CG^CG CelII 304 GCTGAGC GC^T[ACGT]AGC CfoI 1414 GCGC GCG^C EcoRI 1031 GAATTC G^AATTC FbaI 1499 TGATCA T^GATCA HhaI 1414 GCGC GCG^C HinP1I 1414 GCGC G^CGC HincII 491 GTCAAC GT[CT]^[GA]AC HindII 491 GTCAAC GT[CT]^[GA]AC MaeII 1119 ACGT A^CGT MamI 1010 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1226 TGGCCA TGG^CCA MscI 1226 TGGCCA TGG^CCA MvnI 25 CGCG CG^CG NheI 939 GCTAGC G^CTAGC NruI 24 TCGCGA TCG^CGA PaeR7I 1280 CTCGAG C^TCGAG SacI 1211 GAGCTC GAGCT^C ScaI 638 AGTACT AGT^ACT SfiI 327 GGCCGAGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 1280 CTCGAG C^T[CT][GA]AG SphI 815 GCATGC GCATG^C XcmI 1196 CCAAGGTGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1280 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AlwNI 173 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG 646 CAGGGCCTG AspHI 1180 GTGCTC G[AT]GC[AT]^C 1211 GAGCTC BamHI 37 GGATCC G^GATCC 520 GGATCC BcnI 41 CCGGG CC^[GC]GG 671 CCCGG BglI 328 GCCGAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1270 GCCCTGGTGGC BpmI 744 CTCCAG CTGGAG(16/14) 401 CTGGAG BseRI 257 CTCCTC GAGGAG(10/8) 880 GAGGAG BsiHKAI 1180 GTGCTC G[AT]GC[AT]^C 1211 GAGCTC BspMI 1154 ACCTGC ACCTGC(4/8) 702 GCAGGT BsrFI 1058 GCCGGC [GA]^CCGG[CT] 1088 GCCGGC Cfr10I 1058 GCCGGC [GA]^CCGG[CT] 1088 GCCGGC DraII 335 GGGCCCT [GA]G^G[ACGT]CC[CT] 647 AGGGCCT EarI 535 CTCTTC CTCTTC(1/4) 877 GAAGAG EcoNI 339 CCTGAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 837 CCTTAACAAGG EcoO109I 335 GGGCCCT [GA]G^G[ACGT]CC[CT] 647 AGGGCCT Ksp632I 535 CTCTTC CTCTTC(1/4) 877 GAAGAG MseI 476 TTAA T^TAA 839 TTAA MslI 852 CACCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1132 CATGCTGGTG MspA1I 275 CAGCTG C[AC]G^C[GT]G 398 CAGCTG NaeI 1058 GCCGGC GCC^GGC 1088 GCCGGC NciI 41 CCGGG CC^[GC]GG 671 CCCGG NcoI 617 CCATGG C^CATGG 1093 CCATGG NgoMIV 1058 GCCGGC G^CCGGC 1088 GCCGGC NspI 815 GCATGC [GA]CATG^[CT] 1407 ACATGT PflMI 172 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 645 CCAGGGCCTGG PvuII 275 CAGCTG CAG^CTG 398 CAGCTG Tru9I 476 TTAA T^TAA 839 TTAA Tsp509I 954 AATT ^AATT 1032 AATT Van91I 172 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 645 CCAGGGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AfaI AluI AlwI AocI AvaI BanII BbvI BfaI BmyI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DsaI EaeI Eco81I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MspI MvaI MwoI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I XhoII