(Circular) (Six-base) MAPSORT of: pFN21AE5651.seq Check: 8090 from: 1 to: 6274 pFN21AE5651 6274 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2698 2698 Size: 6274 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4084 278 Size: 53 83 186 936 2548 2468 Fragments arranged by size: 2548 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3617 1937 Size: 1680 4594 Acc65I G'GTAC_C Cuts at: 3595 3595 Size: 6274 AclI AA'CG_TT Cuts at: 1563 4401 4774 1563 Size: 2838 373 3063 Fragments arranged by size: 3063 2838 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3026 4337 5721 5989 1632 Size: 1394 1311 1384 268 1917 Fragments arranged by size: 1917 1394 1384 1311 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6077 AflIII A'CryG_T Cuts at: 1203 3412 5173 6150 1203 Size: 2209 1761 977 1327 Fragments arranged by size: 2209 1761 1327 977 AgeI A'CCGG_T Cuts at: 5105 5105 Size: 6274 AhdI GACnn_n'nnGTC Cuts at: 5003 5003 Size: 6274 AleI CACnn'nnGTG Cuts at: 1166 2861 1166 Size: 1695 4579 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3369 1184 Size: 2185 4089 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3428 3460 3428 Size: 32 6242 AlwNI CAG_nnn'CTG Cuts at: 2183 2656 4017 5589 2183 Size: 473 1361 1572 2868 Fragments arranged by size: 2868 1572 1361 473 ApaI G_GGCC'C Cuts at: 2344 2344 Size: 6274 ApaLI G'TGCA_C Cuts at: 4331 5487 4331 Size: 1156 5118 ApoI r'AATT_y Cuts at: 1493 3036 3583 3809 3961 6070 1493 Size: 1543 547 226 152 2109 1697 Fragments arranged by size: 2109 1697 1543 547 226 152 AseI AT'TA_AT Cuts at: 160 1032 4828 160 Size: 872 3796 1606 Fragments arranged by size: 3796 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6274 AvaI C'yCGr_G Cuts at: 1870 1943 2038 2265 3285 3599 1870 Size: 73 95 227 1020 314 4545 Fragments arranged by size: 4545 1020 314 227 95 73 BamHI G'GATC_C Cuts at: 2042 2525 3604 3971 2042 Size: 483 1079 367 4345 Fragments arranged by size: 4345 1079 483 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3595 5050 618 Size: 325 217 627 1808 1455 1842 Fragments arranged by size: 1842 1808 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2344 3220 3292 3593 1391 Size: 953 876 72 301 4072 Fragments arranged by size: 4072 953 876 301 72 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5303 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4465 4499 1903 Size: 34 2528 34 3678 Fragments arranged by size: 3678 2528 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4166 5376 4166 Size: 1210 5064 BclI T'GATC_A Cuts at: 1548 3504 1548 Size: 1956 4318 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2339 3281 4885 Size: 107 122 71 631 605 667 942 1604 Cuts at: 4885 136 Size: 1525 Fragments arranged by size: 1604 1525 942 667 631 605 122 107 71 BglII A'GATC_T Cuts at: 6269 6269 Size: 6274 BlpI GC'TnA_GC Cuts at: 2310 3691 2310 Size: 1381 4893 Bme1580I G_kGCm'C Cuts at: 1790 2261 2344 4335 5491 1790 Size: 471 83 1991 1156 2573 Fragments arranged by size: 2573 1991 1156 471 83 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6274 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2551 2578 4005 Size: 416 53 83 399 24 1134 27 1427 Cuts at: 4005 4963 442 Size: 958 1753 Fragments arranged by size: 1753 1427 1134 958 416 399 83 53 27 24 BmtI G_CTAG'C Cuts at: 1056 2948 1056 Size: 1892 4382 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2203 2203 Size: 6274 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2427 2734 4934 1239 Size: 1188 307 2200 2579 Fragments arranged by size: 2579 2200 1188 307 Bpu10I CC'TnA_GC Cuts at: 2952 2952 Size: 6274 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4399 5264 5562 4399 Size: 865 298 5111 Fragments arranged by size: 5111 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4937 882 Size: 617 3438 2219 Fragments arranged by size: 3438 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4065 493 Size: 711 2861 2702 Fragments arranged by size: 2861 2702 711 BsaBI GATnn'nnATC Cuts at: 3019 6268 3019 Size: 3249 3025 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4081 4463 5098 275 Size: 53 83 186 936 2548 382 635 1451 Fragments arranged by size: 2548 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4707 5105 5379 5526 5937 4707 Size: 398 274 147 411 5044 Fragments arranged by size: 5044 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2879 2909 3399 3429 1178 Size: 30 165 30 1476 30 490 30 4023 Fragments arranged by size: 4023 1476 490 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2253 2900 1186 Size: 1067 647 4560 Fragments arranged by size: 4560 1067 647 BseYI C'CCAG_C Cuts at: 2396 2617 3148 3514 5477 5973 2396 Size: 221 531 366 1963 496 2697 Fragments arranged by size: 2697 1963 531 496 366 221 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3365 1163 Size: 573 1629 4072 Fragments arranged by size: 4072 1629 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3724 4485 5513 1540 Size: 275 186 1723 761 1028 2301 Fragments arranged by size: 2301 1723 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3189 3220 4335 4420 5491 3189 Size: 31 1115 85 1071 3972 Fragments arranged by size: 3972 1115 1071 85 31 BsmI GAATG_Cn' Cuts at: 2247 3785 3878 2247 Size: 1538 93 4643 Fragments arranged by size: 4643 1538 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2438 2438 Size: 6274 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2261 2344 3189 3220 3292 3593 4335 Size: 399 471 83 845 31 72 301 742 Cuts at: 4335 4420 5491 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 845 742 471 399 301 85 83 72 31 BspEI T'CCGG_A Cuts at: 5937 5937 Size: 6274 BspHI T'CATG_A Cuts at: 4163 5893 4163 Size: 1730 4544 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2698 3168 3629 844 Size: 1854 470 461 3489 Fragments arranged by size: 3489 1854 470 461 BsrBI CCG'CTC Cuts at: 1394 1823 2338 4161 1394 Size: 429 515 1823 3507 Fragments arranged by size: 3507 1823 515 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3401 4769 4943 65 Size: 1183 2153 1368 174 1396 Fragments arranged by size: 2153 1396 1368 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3063 3093 4918 5105 1700 Size: 110 1253 30 1825 187 2869 Fragments arranged by size: 2869 1825 1253 187 110 30 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6274 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6274 BssSI C'ACGA_G Cuts at: 4334 5346 4334 Size: 1012 5262 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6274 BstBI TT'CG_AA Cuts at: 5078 5078 Size: 6274 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6274 BstYI r'GATC_y Cuts at: 1972 2042 2525 2755 3496 3604 3971 4356 4373 Size: 70 483 230 741 108 367 385 17 Cuts at: 4373 5825 5911 5923 6269 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 741 483 385 367 346 230 108 86 70 17 12 Bsu36I CC'TnA_GG Cuts at: 2385 2419 2448 2385 Size: 34 29 6211 Fragments arranged by size: 6211 34 29 BtgI C'CryG_G Cuts at: 513 1065 2004 2019 2622 3098 513 Size: 552 939 15 603 476 3689 Fragments arranged by size: 3689 939 603 552 476 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5535 Fragments arranged by size: 5535 683 56 BtsI GCAGTG_nn' Cuts at: 2088 3571 3790 4584 4604 2088 Size: 1483 219 794 20 3758 Fragments arranged by size: 3758 1483 794 219 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6274 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6239 DraI TTT'AAA Cuts at: 3578 3578 Size: 6274 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5281 809 Size: 507 3965 1802 Fragments arranged by size: 3965 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 3095 3231 Size: 54 1425 50 275 18 186 1079 136 Cuts at: 3231 3721 4610 6239 8 Size: 490 889 1629 43 Fragments arranged by size: 1629 1425 1079 889 490 275 186 136 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3721 1537 Size: 275 1909 4090 Fragments arranged by size: 4090 1909 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2546 2877 4204 1353 Size: 1193 331 1327 3423 Fragments arranged by size: 3423 1327 1193 331 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2072 4861 5235 5381 387 Size: 1064 138 483 2789 374 146 1280 Fragments arranged by size: 2789 1280 1064 483 374 146 138 EcoICRI GAG'CTC Cuts at: 3218 3218 Size: 6274 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2427 2734 3026 4337 4934 5721 5989 Size: 393 795 307 292 1311 597 787 268 Cuts at: 5989 1239 Size: 1524 Fragments arranged by size: 1524 1311 795 787 597 393 307 292 268 EcoNI CCTnn'n_nnAGG Cuts at: 2348 2846 2348 Size: 498 5776 EcoO109I rG'GnC_Cy Cuts at: 2341 2653 2341 Size: 312 5962 EcoRI G'AATT_C Cuts at: 3036 3583 3961 3036 Size: 547 378 5349 Fragments arranged by size: 5349 547 378 FspI TGC'GCA Cuts at: 4780 4780 Size: 6274 HaeII r_GCGC'y Cuts at: 1678 5421 1678 Size: 3743 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2027 2059 2510 2517 2542 Size: 32 135 32 455 32 451 7 25 Cuts at: 2542 2549 2879 2911 2985 3017 4915 4947 4989 Size: 7 330 32 74 32 1898 32 42 Cuts at: 4989 5021 1373 Size: 32 2626 Fragments arranged by size: 2626 1898 455 451 330 135 74 42 32 32 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 2498 3618 3864 669 Size: 988 281 560 1120 246 3079 Fragments arranged by size: 3079 1120 988 560 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6274 HpaI GTT'AAC Cuts at: 3864 3864 Size: 6274 KpnI G_GTAC'C Cuts at: 3599 3599 Size: 6274 MfeI C'AATT_G Cuts at: 3873 3873 Size: 6274 MluI A'CGCG_T Cuts at: 6150 6150 Size: 6274 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2267 5388 5572 1305 Size: 962 3121 184 2007 Fragments arranged by size: 3121 2007 962 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3233 10 Size: 54 1425 343 1401 3051 Fragments arranged by size: 3051 1425 1401 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2861 3141 4232 4591 4750 518 Size: 648 1695 280 1091 359 159 2042 Fragments arranged by size: 2042 1695 1091 648 359 280 159 MspA1I CmG'CkG Cuts at: 1606 2282 2405 3690 4367 5515 5760 1606 Size: 676 123 1285 677 1148 245 2120 Fragments arranged by size: 2120 1285 1148 677 676 245 123 NaeI GCC'GGC Cuts at: 3065 3095 3065 Size: 30 6244 NcoI C'CATG_G Cuts at: 513 1065 2004 2622 3098 513 Size: 552 939 618 476 3689 Fragments arranged by size: 3689 939 618 552 476 NdeI CA'TA_TG Cuts at: 387 387 Size: 6274 NgoMIV G'CCGG_C Cuts at: 3063 3093 3063 Size: 30 6244 NheI G'CTAG_C Cuts at: 1052 2944 1052 Size: 1892 4382 NotI GC'GGCC_GC Cuts at: 3721 3721 Size: 6274 NruI TCG'CGA Cuts at: 2031 2031 Size: 6274 NspI r_CATG'y Cuts at: 1132 1229 2824 3416 3632 5177 1132 Size: 97 1595 592 216 1545 2229 Fragments arranged by size: 2229 1595 1545 592 216 97 PasI CC'CwG_GG Cuts at: 1348 2069 2650 1348 Size: 721 581 4972 Fragments arranged by size: 4972 721 581 PciI A'CATG_T Cuts at: 3412 5173 3412 Size: 1761 4513 PflMI CCAn_nnn'nTGG Cuts at: 1108 2183 2656 1108 Size: 1075 473 4726 Fragments arranged by size: 4726 1075 473 PfoI T'CCnGG_A Cuts at: 2045 2045 Size: 6274 PmeI GTTT'AAAC Cuts at: 3578 3578 Size: 6274 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4343 4375 5856 5888 4343 Size: 32 1481 32 4729 Fragments arranged by size: 4729 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6274 PsiI TTA'TAA Cuts at: 3844 3844 Size: 6274 PspOMI G'GGCC_C Cuts at: 2340 2340 Size: 6274 PspXI vC'TCGA_Gb Cuts at: 3285 3285 Size: 6274 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3531 3563 3531 Size: 32 6242 PstI C_TGCA'G Cuts at: 830 2118 2203 2643 3175 3626 830 Size: 1288 85 440 532 451 3478 Fragments arranged by size: 3478 1288 532 451 440 85 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6274 PvuII CAG'CTG Cuts at: 2282 2405 2282 Size: 123 6151 SacI G_AGCT'C Cuts at: 3220 3220 Size: 6274 SalI G'TCGA_C Cuts at: 1936 3616 1936 Size: 1680 4594 SbfI CC_TGCA'GG Cuts at: 3626 3626 Size: 6274 ScaI AGT'ACT Cuts at: 2645 4522 6234 2645 Size: 1877 1712 2685 Fragments arranged by size: 2685 1877 1712 SexAI A'CCwGG_T Cuts at: 3570 3570 Size: 6274 SfcI C'TryA_G Cuts at: 826 1045 2114 2199 2639 3171 3622 4757 5438 Size: 219 1069 85 440 532 451 1135 681 Cuts at: 5438 5629 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1069 681 532 451 440 219 191 85 SfiI GGCCn_nnn'nGGCC Cuts at: 2339 2339 Size: 6274 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6274 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6274 SmaI CCC'GGG Cuts at: 1872 3601 1872 Size: 1729 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3285 4378 5279 5541 820 Size: 197 926 1342 1093 901 262 1553 Fragments arranged by size: 1553 1342 1093 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4065 493 Size: 3572 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6274 SphI G_CATG'C Cuts at: 1132 2824 3632 1132 Size: 1692 808 3774 Fragments arranged by size: 3774 1692 808 SspI AAT'ATT Cuts at: 5 52 4198 5 Size: 47 4146 2081 Fragments arranged by size: 4146 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6274 StyI C'CwwG_G Cuts at: 513 1065 2004 2622 3098 3201 3713 513 Size: 552 939 618 476 103 512 3074 Fragments arranged by size: 3074 939 618 552 512 476 103 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2631 4301 4318 4471 4656 5101 325 Size: 1255 1051 1670 17 153 185 445 1498 Fragments arranged by size: 1670 1498 1255 1051 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2559 2643 4520 Size: 274 80 33 51 1443 582 84 1877 Cuts at: 4520 6232 96 Size: 1712 138 Fragments arranged by size: 1877 1712 1443 582 274 138 84 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6234 6266 6234 Size: 32 6242 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6274 XbaI T'CTAG_A Cuts at: 3610 3610 Size: 6274 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3208 2011 Size: 1197 5077 XhoI C'TCGA_G Cuts at: 1943 3285 1943 Size: 1342 4932 XmaI C'CCGG_G Cuts at: 1870 3599 1870 Size: 1729 4545 XmnI GAAnn'nnTTC Cuts at: 4403 4403 Size: 6274 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4082 276 Size: 53 83 186 936 2548 2468 Fragments arranged by size: 2548 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NsiI PacI PmlI PpuMI RsrII SacII SanDI SapI SfoI SrfI SwaI