################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflIII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AviII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BglI BglII BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT22I FauI FseI FspI HaeII HapII HhaI Hin1I HinP1I HpaI HpaII KasI KpnI Ksp632I KspI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcsI 283 GAATTT [GA]^AATT[CT] AflII 154 CTTAAG C^TTAAG AhdI 521 GACTCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 246 CCTGAGG CC^T[ACGT]AGG ApoI 283 GAATTT [GA]^AATT[CT] AspEI 521 GACTCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 570 GGACC G^G[AT]CC AvrII 654 CCTAGG C^CTAGG BclI 596 TGATCA T^GATCA BfrI 154 CTTAAG C^TTAAG BlnI 654 CCTAGG C^CTAGG BsaJI 654 CCTAGG C^C[ACGT][ACGT]GG BseRI 249 GAGGAG GAGGAG(10/8) BsiYI 62 CCAAAACCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 62 CCAAAACCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BspMI 617 ACCTGC ACCTGC(4/8) BsrI 374 CCAGT ACTGG(1/-1) BstYI 650 AGATCC [GA]^GATC[CT] Bsu36I 246 CCTGAGG CC^T[ACGT]AGG Cfr13I 570 GGACC G^G[ACGT]CC DraII 569 AGGACCT [GA]G^G[ACGT]CC[CT] Eam1105I 521 GACTCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 246 CCTGAGG CC^T[ACGT]AGG EcoO109I 569 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRV 159 GATATC GAT^ATC EcoT14I 654 CCTAGG C^C[AT][AT]GG FbaI 596 TGATCA T^GATCA HaeIII 295 GGCC GG^CC HgaI 354 GCGTC GACGC(5/10) HincII 533 GTCAAC GT[CT]^[GA]AC HindII 533 GTCAAC GT[CT]^[GA]AC HindIII 148 AAGCTT A^AGCTT MflI 650 AGATCC [GA]^GATC[CT] MslI 670 CACGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 592 CAGCTG C[AC]G^C[GT]G PpuMI 569 AGGACCT [GA]G^G[AT]CC[CT] PvuII 592 CAGCTG CAG^CTG Sau96I 570 GGACC G^G[ACGT]CC SmlI 154 CTTAAG C^T[CT][GA]AG SspI 476 AATATT AAT^ATT StyI 654 CCTAGG C^C[AT][AT]GG TaqI 519 TCGA T^CGA TspRI 498 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 519 TCGA T^CGA XhoII 650 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AlwI 651 GATCC GGATC(4/5) 210 GGATC AlwNI 241 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG 330 CAGGAACTG BbsI 14 GAAGAC GAAGAC(2/6) 319 GAAGAC BfaI 202 CTAG C^TAG 655 CTAG BpmI 608 CTCCAG CTGGAG(16/14) 298 CTGGAG BpuAI 14 GAAGAC GAAGAC(2/6) 319 GAAGAC BsmAI 252 GAGAC GTCTC(1/5) 401 GTCTC BssKI 68 CCAGG ^CC[ACGT]GG 297 CCTGG BstNI 68 CCAGG CC^[AT]GG 297 CCTGG Cac8I 438 GCCTGC GC[ACGT]^[ACGT]GC 669 GCACGC EcoRII 68 CCAGG ^CC[AT]GG 297 CCTGG HphI 575 TCACC GGTGA(8/7) 635 TCACC MaeI 202 CTAG C^TAG 655 CTAG MseI 155 TTAA T^TAA 176 TTAA MvaI 68 CCAGG CC^[AT]GG 297 CCTGG NlaIII 588 CATG CATG^ 625 CATG ScrFI 68 CCAGG CC^[ACGT]GG 297 CCTGG TfiI 195 GAATC G^A[AT]TC 661 GATTC Tru9I 155 TTAA T^TAA 176 TTAA Tsp509I 168 AATT ^AATT 284 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AluI BbvI BstF5I DdeI DpnII Fnu4HI FokI HinfI ItaI MboI MboII MnlI NdeII PleI PstI Sau3AI SfaNI SfcI TseI