################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BfaI BfrI BlnI BlpI Bpu1102I BsaBI BsaI BsiWI BsmI BspLU11I BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII CpoI DraI DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FseI HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MseI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Tru9I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccII 2274 CGCG CG^CG AccIII 2204 TCCGGA T^CCGGA AgeI 807 ACCGGT A^CCGGT AlwNI 2301 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG AspI 121 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1586 TGCGCA TGC^GCA BamHI 1616 GGATCC G^GATCC BbeI 231 GGCGCC GGCGC^C BbrPI 1999 CACGTG CAC^GTG BcgI 2351 GCATCCTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2454 GGATAC GTATCC(6/5) BclI 1394 TGATCA T^GATCA BglI 1822 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 957 CTGGAG CTGGAG(16/14) BseAI 2204 TCCGGA T^CCGGA BsmBI 81 CGTCTC CGTCTC(1/5) Bsp1407I 1739 TGTACA T^GTACA BspDI 1237 ATCGAT AT^CGAT BspEI 2204 TCCGGA T^CCGGA BspHI 2195 TCATGA T^CATGA BsrBI 2038 CCGCTC CCGCTC(-3/-3) BsrGI 1739 TGTACA T^GTACA BstAPI 2243 GCATCGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 2274 CGCG CG^CG ClaI 1237 ATCGAT AT^CGAT DrdI 1246 GACAAGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 2278 CTCTTC CTCTTC(1/4) FbaI 1394 TGATCA T^GATCA FspI 1586 TGCGCA TGC^GCA HgaI 94 GACGC GACGC(5/10) KasI 231 GGCGCC G^GCGCC Ksp632I 2278 CTCTTC CTCTTC(1/4) MroI 2204 TCCGGA T^CCGGA MvnI 2274 CGCG CG^CG NarI 231 GGCGCC GG^CGCC NcoI 1646 CCATGG C^CATGG PaeR7I 1034 CTCGAG C^TCGAG PflFI 121 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 807 ACCGGT A^CCGGT PmaCI 1999 CACGTG CAC^GTG PmlI 1999 CACGTG CAC^GTG PstI 2321 CTGCAG CTGCA^G PvuII 1387 CAGCTG CAG^CTG RcaI 2195 TCATGA T^CATGA SapI 2277 GCTCTTC GCTCTTC(1/4) ScaI 515 AGTACT AGT^ACT SfoI 231 GGCGCC GGC^GCC SphI 1760 GCATGC GCATG^C SspBI 1739 TGTACA T^GTACA StuI 578 AGGCCT AGG^CCT Tth111I 121 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1193 CCATGAGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1034 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 94 GACGCC G[GA]^CG[CT]C 231 GGCGCC Aor51HI 1012 AGCGCT AGC^GCT 1135 AGCGCT ApaI 70 GGGCCC GGGCC^C 1577 GGGCCC BbvCI 1500 CCTCAGC CCTCAGC(-5/-2) 1932 CCTCAGC BglII 1331 AGATCT A^GATCT 2474 AGATCT BsaHI 94 GACGCC G[GA]^CG[CT]C 231 GGCGCC BsaWI 807 ACCGGT [AT]^CCGG[AT] 2204 TCCGGA BsiEI 44 CGGTCG CG[GA][CT]^CG 809 CGGTCG BsmAI 82 GTCTC GTCTC(1/5) 852 GTCTC BspMI 923 ACCTGC ACCTGC(4/8) 2165 ACCTGC BsrFI 807 ACCGGT [GA]^CCGG[CT] 1958 GCCGGT Cfr10I 807 ACCGGT [GA]^CCGG[CT] 1958 GCCGGT DsaI 1646 CCATGG C^C[GA][CT]GG 2258 CCGTGG Eco47III 1012 AGCGCT AGC^GCT 1135 AGCGCT EcoT14I 1023 CCTTGG C^C[AT][AT]GG 1646 CCATGG Hin1I 94 GACGCC G[GA]^CG[CT]C 231 GGCGCC NspI 659 GCATGT [GA]CATG^[CT] 1760 GCATGC PpuMI 1148 GGGACCT [GA]G^G[AT]CC[CT] 2416 GGGTCCT SacI 529 GAGCTC GAGCT^C 1197 GAGCTC SfcI 1809 CTACAG C^T[GA][CT]AG 2321 CTGCAG SmlI 854 CTCAAG C^T[CT][GA]AG 1034 CTCGAG StyI 1023 CCTTGG C^C[AT][AT]GG 1646 CCATGG Tsp509I 555 AATT ^AATT 1714 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BmyI BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrDI BsrI BssKI BstEII BstF5I BstNI BstPI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoO65I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XhoII