################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII Acc65I AcyI AgeI Aor51HI ApaI AscI Asp718I AspI AviII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BglI BsaHI BsiWI BsmBI Bsp1407I BspDI BsrGI BssHII BssSI BstAPI BstEII BstPI BstXI ClaI CpoI DraIII Eco47III EcoO65I EcoRI EcoT22I FseI FspI HaeII HgaI Hin1I HpaI KasI KpnI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PvuI PvuII RsrII SacI SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AccI 3117 GTATAC GT^[AC][GT]AC AclI 1463 AACGTT AA^CGTT AflII 1309 CTTAAG C^TTAAG AflIII 2226 ACATGT A^C[GA][CT]GT AhdI 1098 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3050 GTGCAC G^TGCAC AlwNI 881 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 582 CCTCAGG CC^T[ACGT]AGG ApaLI 3050 GTGCAC G^TGCAC AseI 1554 ATTAAT AT^TAAT AsnI 1554 ATTAAT AT^TAAT AspEI 1098 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 213 CCTAGG C^CTAGG BclI 2900 TGATCA T^GATCA BfrI 1309 CTTAAG C^TTAAG BglII 1560 AGATCT A^GATCT BlnI 213 CCTAGG C^CTAGG BlpI 2073 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2073 GCTCAGC GC^T[ACGT]AGC BsaAI 3061 CACGTA [CT]AC^GT[GA] BsaBI 1270 GATCACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 160 GAGACC GGTCTC(1/5) BsgI 2809 GTGCAG GTGCAG(16/14) BsiEI 8 CGGCCG CG[GA][CT]^CG BsmI 2955 GCATTC GAATGC(1/-1) BspHI 1590 TCATGA T^CATGA BspLU11I 2226 ACATGT A^CATGT BspMI 425 ACCTGC ACCTGC(4/8) BsrBI 521 CCGCTC CCGCTC(-3/-3) BsrDI 2990 CATTGC GCAATG(2/0) Bst1107I 3117 GTATAC GTA^TAC BstBI 3026 TTCGAA TT^CGAA BstZ17I 3117 GTATAC GTA^TAC Bsu36I 582 CCTCAGG CC^T[ACGT]AGG CelII 2073 GCTCAGC GC^T[ACGT]AGC DrdI 2708 GACTCATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 8 CGGCCG C^GGCCG Eam1105I 1098 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 8 CGGCCG C^GGCCG Eco52I 8 CGGCCG C^GGCCG Eco81I 582 CCTCAGG CC^T[ACGT]AGG EcoRV 2005 GATATC GAT^ATC FbaI 2900 TGATCA T^GATCA KspI 116 CCGCGG CCGC^GG MamI 1270 GATCACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 16 GCCGGC GCC^GGC NcoI 1056 CCATGG C^CATGG NgoMIV 16 GCCGGC G^CCGGC NspV 3026 TTCGAA TT^CGAA PshBI 1554 ATTAAT AT^TAAT Psp1406I 1463 AACGTT AA^CGTT RcaI 1590 TCATGA T^CATGA SacII 116 CCGCGG CCGC^GG ScaI 1344 AGTACT AGT^ACT SfuI 3026 TTCGAA TT^CGAA SmlI 1309 CTTAAG C^T[CT][GA]AG SphI 2049 GCATGC GCATG^C StuI 984 AGGCCT AGG^CCT Tsp45I 467 GTGAC ^GT[GC]AC XbaI 2959 TCTAGA T^CTAGA XcmI 529 CCAACTAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 66 CGCG CG^CG 117 CGCG AccIII 1169 TCCGGA T^CCGGA 2940 TCCGGA AspHI 2126 GAGCAC G[AT]GC[AT]^C 3050 GTGCAC AvaI 382 CTCGGG C^[CT]CG[GA]G 448 CTCGGG BalI 506 TGGCCA TGG^CCA 2255 TGGCCA BanII 75 GGGCTC G[GA]GC[CT]^C 2848 GAGCCC BbsI 728 GAAGAC GAAGAC(2/6) 997 GAAGAC BpuAI 728 GAAGAC GAAGAC(2/6) 997 GAAGAC BseAI 1169 TCCGGA T^CCGGA 2940 TCCGGA BsiHKAI 2126 GAGCAC G[AT]GC[AT]^C 3050 GTGCAC BsoBI 382 CTCGGG C^[CT]CG[GA]G 448 CTCGGG BspEI 1169 TCCGGA T^CCGGA 2940 TCCGGA BstUI 66 CGCG CG^CG 117 CGCG CfoI 65 GCGC GCG^C 2019 GCGC EcoNI 582 CCTCAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1767 CCTTCGTCAGG EcoT14I 213 CCTAGG C^C[AT][AT]GG 1056 CCATGG HhaI 65 GCGC GCG^C 2019 GCGC HinP1I 65 GCGC G^CGC 2019 GCGC HincII 516 GTTGAC GT[CT]^[GA]AC 679 GTCAAC HindII 516 GTTGAC GT[CT]^[GA]AC 679 GTCAAC MluNI 506 TGGCCA TGG^CCA 2255 TGGCCA MroI 1169 TCCGGA T^CCGGA 2940 TCCGGA MscI 506 TGGCCA TGG^CCA 2255 TGGCCA MspA1I 71 CAGCGG C[AC]G^C[GT]G 116 CCGCGG MvnI 66 CGCG CG^CG 117 CGCG NspI 2049 GCATGC [GA]CATG^[CT] 2226 ACATGT PpuMI 640 GGGTCCT [GA]G^G[AT]CC[CT] 2110 GGGTCCC PstI 1113 CTGCAG CTGCA^G 1714 CTGCAG SspI 1183 AATATT AAT^ATT 2574 AATATT StyI 213 CCTAGG C^C[AT][AT]GG 1056 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AvaII BbvI BcnI BfaI BmyI BpmI BsaJI BsaWI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII XmnI