################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AhdI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaAI BsaI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeI MaeII MfeI MluI MluNI MroI MscI MseI MunI NarI NcoI NdeI NheI NotI NsiI NspI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tru9I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccII 134 CGCG CG^CG AcyI 67 GGCGTC G[GA]^CG[CT]C AfaI 341 GTAC GT^AC AgeI 154 ACCGGT A^CCGGT Alw44I 127 GTGCAC G^TGCAC AlwNI 238 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 228 CCTGAGG CC^T[ACGT]AGG ApaLI 127 GTGCAC G^TGCAC AspHI 127 GTGCAC G[AT]GC[AT]^C BanII 97 GAGCCC G[GA]GC[CT]^C BbvCI 303 CCTCAGC CCTCAGC(-5/-2) BglI 4 GCCCGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 78 GCTCAGC GC^T[ACGT]AGC BpmI 181 CTGGAG CTGGAG(16/14) Bpu1102I 78 GCTCAGC GC^T[ACGT]AGC BsaBI 452 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 67 GGCGTC G[GA]^CG[CT]C BsaWI 154 ACCGGT [AT]^CCGG[AT] BsiHKAI 127 GTGCAC G[AT]GC[AT]^C BspHI 255 TCATGA T^CATGA BsrBI 76 CCGCTC CCGCTC(-3/-3) BstUI 134 CGCG CG^CG Bsu36I 228 CCTGAGG CC^T[ACGT]AGG CelII 78 GCTCAGC GC^T[ACGT]AGC CfoI 20 GCGC GCG^C DraII 427 GGGACCC [GA]G^G[ACGT]CC[CT] Eco81I 228 CCTGAGG CC^T[ACGT]AGG EcoNI 224 CCTCCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 427 GGGACCC [GA]G^G[ACGT]CC[CT] HaeII 19 GGCGCT [GA]GCGC^[CT] HgaI 68 GCGTC GACGC(5/10) HhaI 20 GCGC GCG^C Hin1I 67 GGCGTC G[GA]^CG[CT]C HinP1I 20 GCGC G^CGC MaeIII 158 GTCAC ^GT[ACGT]AC MamI 452 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 446 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 134 CGCG CG^CG NaeI 64 GCCGGC GCC^GGC NgoMIV 64 GCCGGC G^CCGGC NruI 133 TCGCGA TCG^CGA PinAI 154 ACCGGT A^CCGGT PpuMI 427 GGGACCC [GA]G^G[AT]CC[CT] RcaI 255 TCATGA T^CATGA RsaI 341 GTAC GT^AC SapI 45 GCTCTTC GCTCTTC(1/4) TfiI 269 GAATC G^A[AT]TC Tsp45I 158 GTCAC ^GT[GC]AC XcmI 327 CCAGGAGGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwI 120 GATCC GGATC(4/5) 87 GGATC AvaII 234 GGACC G^G[AT]CC 428 GGACC BcnI 5 CCCGG CC^[GC]GG 93 CCCGG BsaJI 408 CCCAGG C^C[ACGT][ACGT]GG 409 CCAGGG BsrFI 64 GCCGGC [GA]^CCGG[CT] 154 ACCGGT BstNI 327 CCAGG CC^[AT]GG 409 CCAGG BstYI 87 GGATCT [GA]^GATC[CT] 119 AGATCC Cfr10I 64 GCCGGC [GA]^CCGG[CT] 154 ACCGGT EarI 46 CTCTTC CTCTTC(1/4) 174 GAAGAG EcoRII 327 CCAGG ^CC[AT]GG 409 CCAGG Ksp632I 46 CTCTTC CTCTTC(1/4) 174 GAAGAG MboII 174 GAAGA GAAGA(8/7) 47 TCTTC MflI 87 GGATCT [GA]^GATC[CT] 119 AGATCC MspA1I 81 CAGCTG C[AC]G^C[GT]G 247 CAGCTG MvaI 327 CCAGG CC^[AT]GG 409 CCAGG NciI 5 CCCGG CC^[GC]GG 93 CCCGG NlaIII 165 CATG CATG^ 256 CATG PleI 177 GAGTC GAGTC(4/5) 252 GAGTC PstI 244 CTGCAG CTGCA^G 350 CTGCAG PvuII 81 CAGCTG CAG^CTG 247 CAGCTG SfcI 244 CTGCAG C^T[GA][CT]AG 350 CTGCAG XhoII 87 GGATCT [GA]^GATC[CT] 119 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AluI BbvI BmyI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I Cac8I Cfr13I DdeI DpnII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MboI MnlI MspI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI TseI