################################## # NO CUT ENZYMES ( 221 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DpnII DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI Fnu4HI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI ItaI KasI KpnI KspI MaeI MaeII MaeIII MamI MboI MfeI MflI MluI MluNI MscI MslI MspA1I MunI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI Sau3AI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TseI Tsp45I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AccIII 84 TCCGGA T^CCGGA AciI 60 CCGC CCGC(-3/-1) AcsI 119 AAATTC [GA]^AATT[CT] ApoI 119 AAATTC [GA]^AATT[CT] BsaWI 84 TCCGGA [AT]^CCGG[AT] BseAI 84 TCCGGA T^CCGGA BspEI 84 TCCGGA T^CCGGA BssKI 105 CCAGG ^CC[ACGT]GG BstF5I 111 GGATG GGATG(2/0) BstNI 105 CCAGG CC^[AT]GG DraI 70 TTTAAA TTT^AAA EarI 20 CTCTTC CTCTTC(1/4) EcoRII 105 CCAGG ^CC[AT]GG FauI 59 CCCGC CCCGC(4/6) FokI 111 GGATG GGATG(9/13) HapII 85 CCGG C^CGG HpaII 85 CCGG C^CGG Ksp632I 20 CTCTTC CTCTTC(1/4) MboII 21 TCTTC GAAGA(8/7) MroI 84 TCCGGA T^CCGGA MseI 71 TTAA T^TAA MspI 85 CCGG C^CGG MvaI 105 CCAGG CC^[AT]GG NdeI 200 CATATG CA^TATG NlaIII 174 CATG CATG^ SapI 19 GCTCTTC GCTCTTC(1/4) ScrFI 105 CCAGG CC^[ACGT]GG SfaNI 151 GATGC GCATC(5/9) Tru9I 71 TTAA T^TAA Tsp509I 120 AATT ^AATT XcmI 206 CCATATATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 4 enzymes) # ################################### AluI 147 AGCT AG^CT 215 AGCT HinfI 9 GAATC G^A[ACGT]TC 177 GATTC TfiI 9 GAATC G^A[AT]TC 177 GATTC TspRI 51 CACTG CA[GC]TG[ACGT][ACGT]^ 125 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### Cac8I MnlI