################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI Alw44I ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AviII AvrII BanI BanII BbeI BbvCI BcgI BglI BlnI BlpI BmyI BpmI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII ClaI CpoI DraI DraIII DrdI DsaI EagI EarI EclXI Eco52I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FseI FspI HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MspA1I MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StyI SwaI Tsp45I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 94 GTAGAC GT^[AC][GT]AC AfaI 708 GTAC GT^AC AflII 75 CTTAAG C^TTAAG AflIII 85 ACGTGT A^C[GA][CT]GT AhdI 436 GACCGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 910 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 941 AGCGCT AGC^GCT AspEI 436 GACCGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 366 GGATCC G^GATCC BbrPI 84 CACGTG CAC^GTG BciVI 900 GGATAC GTATCC(6/5) BclI 830 TGATCA T^GATCA BcnI 857 CCCGG CC^[GC]GG BfaI 721 CTAG C^TAG BfrI 75 CTTAAG C^TTAAG BglII 346 AGATCT A^GATCT BsaAI 84 CACGTG [CT]AC^GT[GA] BseRI 577 GAGGAG GAGGAG(10/8) BsmAI 144 GTCTC GTCTC(1/5) BsmFI 104 GGGAC GGGAC(10/14) Bsp1407I 707 TGTACA T^GTACA BspMI 386 ACCTGC ACCTGC(4/8) BsrBI 967 GAGCGG CCGCTC(-3/-3) BsrFI 562 GCCGGC [GA]^CCGG[CT] BsrGI 707 TGTACA T^GTACA BstAPI 668 GCATAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 716 CCATCCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 942 GCGC GCG^C Cfr10I 562 GCCGGC [GA]^CCGG[CT] DraII 581 AGGCCCT [GA]G^G[ACGT]CC[CT] Eam1105I 436 GACCGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 941 AGCGCT AGC^GCT EcoO109I 581 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRI 776 GAATTC G^AATTC FbaI 830 TGATCA T^GATCA HaeII 941 AGCGCT [GA]GCGC^[CT] HhaI 942 GCGC GCG^C HinP1I 942 GCGC G^CGC MaeI 721 CTAG C^TAG MaeIII 960 GTTAC ^GT[ACGT]AC MseI 76 TTAA T^TAA MslI 717 CATCCTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 562 GCCGGC GCC^GGC NciI 857 CCCGG CC^[GC]GG NgoMIV 562 GCCGGC G^CCGGC NspI 956 GCATGT [GA]CATG^[CT] PmaCI 84 CACGTG CAC^GTG PmlI 84 CACGTG CAC^GTG RsaI 708 GTAC GT^AC SspBI 707 TGTACA T^GTACA StuI 838 AGGCCT AGG^CCT Tru9I 76 TTAA T^TAA XcmI 716 CCATCCTAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 760 GAATTT [GA]^AATT[CT] 776 GAATTC AocI 361 CCTGAGG CC^T[ACGT]AGG 841 CCTAAGG ApoI 760 GAATTT [GA]^AATT[CT] 776 GAATTC BalI 392 TGGCCA TGG^CCA 483 TGGCCA BbsI 111 GAAGAC GAAGAC(2/6) 606 GAAGAC BpuAI 111 GAAGAC GAAGAC(2/6) 606 GAAGAC BsgI 539 CTGCAC GTGCAG(16/14) 590 CTGCAC BsrI 246 CCAGT ACTGG(1/-1) 486 CCAGT Bsu36I 361 CCTGAGG CC^T[ACGT]AGG 841 CCTAAGG Eco81I 361 CCTGAGG CC^T[ACGT]AGG 841 CCTAAGG FauI 298 CCCGC CCCGC(4/6) 969 GCGGG HapII 563 CCGG C^CGG 858 CCGG HpaII 563 CCGG C^CGG 858 CCGG MaeII 85 ACGT A^CGT 98 ACGT MluNI 392 TGGCCA TGG^CCA 483 TGGCCA MscI 392 TGGCCA TGG^CCA 483 TGGCCA MspI 563 CCGG C^CGG 858 CCGG PleI 509 GACTC GAGTC(4/5) 46 GAGTC PstI 271 CTGCAG CTGCA^G 766 CTGCAG SfaNI 161 GCATC GCATC(5/9) 448 GCATC SfcI 271 CTGCAG C^T[GA][CT]AG 766 CTGCAG SmlI 75 CTTAAG C^T[CT][GA]AG 755 CTCAAG TaqI 216 TCGA T^CGA 252 TCGA TthHB8I 216 TCGA T^CGA 252 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AluI AlwI AvaII BbvI BsaJI BsiYI BslI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HinfI ItaI MboI MboII MflI MnlI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TfiI TseI Tsp509I TspRI XhoII