################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MflI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 791 GGTACC G^GTACC AccI 765 GTATAC GT^[AC][GT]AC AciI 106 CCGC CCGC(-3/-1) AseI 930 ATTAAT AT^TAAT AsnI 930 ATTAAT AT^TAAT Asp718I 791 GGTACC G^GTACC AvaII 540 GGACC G^G[AT]CC BanI 791 GGTACC G^G[CT][GA]CC BbsI 89 GTCTTC GAAGAC(2/6) BciVI 863 GTATCC GTATCC(6/5) BfaI 925 CTAG C^TAG BpmI 338 CTGGAG CTGGAG(16/14) BpuAI 89 GTCTTC GAAGAC(2/6) BseRI 128 GAGGAG GAGGAG(10/8) BsiEI 760 CGGCCG CG[GA][CT]^CG BsiYI 211 CCTCGCTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 211 CCTCGCTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 612 GAGAC GTCTC(1/5) BsmFI 555 GGGAC GGGAC(10/14) Bst1107I 765 GTATAC GTA^TAC BstZ17I 765 GTATAC GTA^TAC EaeI 760 CGGCCG [CT]^GGCC[GA] EagI 760 CGGCCG C^GGCCG EarI 877 CTCTTC CTCTTC(1/4) EclXI 760 CGGCCG C^GGCCG Eco52I 760 CGGCCG C^GGCCG EcoRV 274 GATATC GAT^ATC EcoT14I 867 CCTTGG C^C[AT][AT]GG HapII 426 CCGG C^CGG HpaII 426 CCGG C^CGG HphI 220 GGTGA GGTGA(8/7) KpnI 791 GGTACC GGTAC^C Ksp632I 877 CTCTTC CTCTTC(1/4) MaeI 925 CTAG C^TAG MaeII 366 ACGT A^CGT MslI 580 CATTTTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 106 CCGCTG C[AC]G^C[GT]G MspI 426 CCGG C^CGG MwoI 111 GCCCCCTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 791 GGTACC GG[ACGT]^[ACGT]CC PleI 147 GAGTC GAGTC(4/5) PshBI 930 ATTAAT AT^TAAT SfaNI 484 GATGC GCATC(5/9) SspI 40 AATATT AAT^ATT StuI 193 AGGCCT AGG^CCT StyI 867 CCTTGG C^C[AT][AT]GG TfiI 565 GAATC G^A[AT]TC Tsp45I 469 GTGAC ^GT[GC]AC TspRI 336 CACTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 268 AAATTT [GA]^AATT[CT] 419 AAATTC ApoI 268 AAATTT [GA]^AATT[CT] 419 AAATTC Asp700I 38 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 385 GAAGGCTTTC BsaJI 323 CCAGGG C^C[ACGT][ACGT]GG 867 CCTTGG BssKI 124 CCAGG ^CC[ACGT]GG 323 CCAGG BstNI 124 CCAGG CC^[AT]GG 323 CCAGG Cfr13I 208 GGGCC G^G[ACGT]CC 540 GGACC DdeI 116 CTGAG C^T[ACGT]AG 747 CTCAG DpnII 136 GATC ^GATC 547 GATC EcoRII 124 CCAGG ^CC[AT]GG 323 CCAGG HinfI 147 GAGTC G^A[ACGT]TC 565 GAATC MaeIII 177 GTTAC ^GT[ACGT]AC 469 GTGAC MboI 136 GATC ^GATC 547 GATC MvaI 124 CCAGG CC^[AT]GG 323 CCAGG NdeII 136 GATC ^GATC 547 GATC Sau3AI 136 GATC ^GATC 547 GATC Sau96I 208 GGGCC G^G[ACGT]CC 540 GGACC ScrFI 124 CCAGG CC^[ACGT]GG 323 CCAGG TaqI 408 TCGA T^CGA 735 TCGA TthHB8I 408 TCGA T^CGA 735 TCGA XmnI 38 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 385 GAAGGCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AfaI AluI BbvI BsrI Fnu4HI HaeIII ItaI MboII MnlI MseI NlaIII RsaI Tru9I TseI Tsp509I