################################## # NO CUT ENZYMES ( 185 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI AluI AlwNI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI Cac8I CelII CfoI Cfr10I CpoI DraI DraII DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MboII MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI TseI Tsp45I Tsp509I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 37 GTATAC GT^[AC][GT]AC Alw44I 48 GTGCAC G^TGCAC AocI 42 CCTGAGG CC^T[ACGT]AGG ApaLI 48 GTGCAC G^TGCAC AspHI 48 GTGCAC G[AT]GC[AT]^C BclI 140 TGATCA T^GATCA BmyI 48 GTGCAC G[AGT]GC[ACT]^C BsaWI 52 ACCGGA [AT]^CCGG[AT] BsiHKAI 48 GTGCAC G[AT]GC[AT]^C BsmAI 170 GAGAC GTCTC(1/5) BsmFI 301 GGGAC GGGAC(10/14) BsmI 382 GAATGC GAATGC(1/-1) Bsp1286I 48 GTGCAC G[AGT]GC[ACT]^C BspDI 355 ATCGAT AT^CGAT BspHI 345 TCATGA T^CATGA BsrDI 234 CATTGC GCAATG(2/0) Bst1107I 37 GTATAC GTA^TAC BstF5I 340 CATCC GGATG(2/0) BstYI 293 AGATCC [GA]^GATC[CT] BstZ17I 37 GTATAC GTA^TAC Bsu36I 42 CCTGAGG CC^T[ACGT]AGG ClaI 355 ATCGAT AT^CGAT DrdI 303 GACTGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 333 TGGCCG [CT]^GGCC[GA] Eco81I 42 CCTGAGG CC^T[ACGT]AGG FbaI 140 TGATCA T^GATCA FokI 340 CATCC GGATG(9/13) HapII 53 CCGG C^CGG HincII 229 GTCAAC GT[CT]^[GA]AC HindII 229 GTCAAC GT[CT]^[GA]AC HpaII 53 CCGG C^CGG HphI 58 GGTGA GGTGA(8/7) MflI 293 AGATCC [GA]^GATC[CT] MslI 340 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 53 CCGG C^CGG MwoI 70 GCCACTTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIII 346 CATG CATG^ PflMI 309 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 345 TCATGA T^CATGA SfaNI 246 GATGC GCATC(5/9) SmlI 154 CTCAAG C^T[CT][GA]AG Van91I 309 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 71 CCACTTCTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 293 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### AlwI 257 GATCC GGATC(4/5) 294 GATCC BsiYI 88 CCCAAGATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 309 CCAGTCAGTGG BslI 88 CCCAAGATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 309 CCAGTCAGTGG BsrI 309 CCAGT ACTGG(1/-1) 392 CCAGT Cfr13I 84 GGCCC G^G[ACGT]CC 97 GGCCC DdeI 43 CTGAG C^T[ACGT]AG 261 CTTAG MseI 194 TTAA T^TAA 287 TTAA Sau96I 84 GGCCC G^G[ACGT]CC 97 GGCCC Tru9I 194 TTAA T^TAA 287 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AciI BanI BsaJI BssKI BstNI DpnII EcoRII Fnu4HI HaeIII ItaI MboI MnlI MvaI NdeII NlaIV Sau3AI ScrFI TaqI TspRI TthHB8I