Restriction map for
pFN21AE5190
(Circular) (Six-base) MAPSORT of: pFN21AE5190.seq Check: 3369 from: 1 to: 5101
pFN21AE5190 5101 bp
With 182 enzymes: SgfI *
January 30, 2014 17:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2911 278
Size: 53 83 186 936 1375 2468
Fragments arranged by size:
2468 1375 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2043 2444 1937
Size: 106 401 4594
Fragments arranged by size:
4594 401 106
Acc65I G'GTAC_C
Cuts at: 2422 2422
Size: 5101
AclI AA'CG_TT
Cuts at: 1563 3228 3601 1563
Size: 1665 373 3063
Fragments arranged by size:
3063 1665 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3164 4548 4816 1632
Size: 1532 1384 268 1917
Fragments arranged by size:
1917 1532 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4904
AflIII A'CryG_T
Cuts at: 1203 4000 4977 1203
Size: 2797 977 1327
Fragments arranged by size:
2797 1327 977
AgeI A'CCGG_T
Cuts at: 3932 3932
Size: 5101
AhdI GACnn_n'nnGTC
Cuts at: 3830 3830
Size: 5101
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5101
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5101
AlwNI CAG_nnn'CTG
Cuts at: 2844 4416 2844
Size: 1572 3529
ApaLI G'TGCA_C
Cuts at: 2053 3158 4314 2053
Size: 1105 1156 2840
Fragments arranged by size:
2840 1156 1105
ApoI r'AATT_y
Cuts at: 1493 2410 2636 2788 4897 1493
Size: 917 226 152 2109 1697
Fragments arranged by size:
2109 1697 917 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3655 160
Size: 872 2623 1606
Fragments arranged by size:
2623 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5101
AvaI C'yCGr_G
Cuts at: 1870 1943 2426 1870
Size: 73 483 4545
Fragments arranged by size:
4545 483 73
BamHI G'GATC_C
Cuts at: 2431 2798 2431
Size: 367 4734
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2072 2125 2256 2422 3877
Size: 325 217 627 285 53 131 166 1455
Cuts at: 3877 618
Size: 1842
Fragments arranged by size:
1842 1455 627 325 285 217 166 131
53
BanII G_rGCy'C
Cuts at: 1391 2420 1391
Size: 1029 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3292 3326 1903
Size: 34 50 34 1271 34 3678
Fragments arranged by size:
3678 1271 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2993 4203 2993
Size: 1210 3891
BclI T'GATC_A
Cuts at: 1548 2145 1548
Size: 597 4504
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3712 136
Size: 107 122 71 631 605 2040 1525
Fragments arranged by size:
2040 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5096 5096
Size: 5101
BlpI GC'TnA_GC
Cuts at: 2518 2518
Size: 5101
Bme1580I G_kGCm'C
Cuts at: 1790 2057 3162 4318 1790
Size: 267 1105 1156 2573
Fragments arranged by size:
2573 1156 1105 267
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5101
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2308 2832 3790
Size: 416 53 83 399 24 891 524 958
Cuts at: 3790 442
Size: 1753
Fragments arranged by size:
1753 958 891 524 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3761 1239
Size: 2522 2579
Bpu10I CC'TnA_GC
Cuts at: 2266 2266
Size: 5101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2144 3226 4091 4389 2144
Size: 1082 865 298 2856
Fragments arranged by size:
2856 1082 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3764 882
Size: 617 2265 2219
Fragments arranged by size:
2265 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2892 493
Size: 711 1688 2702
Fragments arranged by size:
2702 1688 711
BsaBI GATnn'nnATC
Cuts at: 5095 5095
Size: 5101
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2908 3290 3925 275
Size: 53 83 186 936 1375 382 635 1451
Fragments arranged by size:
1451 1375 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2057 3534 3932 4206 4353 4764 2057
Size: 1477 398 274 147 411 2394
Fragments arranged by size:
2394 1477 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2052 2082 1178
Size: 30 165 30 649 30 4197
Fragments arranged by size:
4197 649 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5101
BseYI C'CCAG_C
Cuts at: 2392 4304 4800 2392
Size: 1912 496 2693
Fragments arranged by size:
2693 1912 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4528
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2551 3312 4340 1540
Size: 275 186 550 761 1028 2301
Fragments arranged by size:
2301 1028 761 550 275 186
BsiHKAI G_wGCw'C
Cuts at: 2057 3162 3247 4318 2057
Size: 1105 85 1071 2840
Fragments arranged by size:
2840 1105 1071 85
BsmI GAATG_Cn'
Cuts at: 2393 2612 2705 2393
Size: 219 93 4789
Fragments arranged by size:
4789 219 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2420 3162 3247 4318 1391
Size: 399 267 363 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 363 267 85
BspEI T'CCGG_A
Cuts at: 4764 4764
Size: 5101
BspHI T'CATG_A
Cuts at: 2350 2990 4720 2350
Size: 640 1730 2731
Fragments arranged by size:
2731 1730 640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2456 844
Size: 1612 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2988 1394
Size: 429 1165 3507
Fragments arranged by size:
3507 1165 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2238 3596 3770 65
Size: 1183 990 1358 174 1396
Fragments arranged by size:
1396 1358 1183 990 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3745 3932 1700
Size: 110 1935 187 2869
Fragments arranged by size:
2869 1935 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5101
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5101
BssSI C'ACGA_G
Cuts at: 3161 4173 3161
Size: 1012 4089
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5101
BstBI TT'CG_AA
Cuts at: 3905 3905
Size: 5101
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5101
BstYI r'GATC_y
Cuts at: 1972 2298 2431 2798 3183 3200 4652 4738 4750
Size: 326 133 367 385 17 1452 86 12
Cuts at: 4750 5096 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 326 133 86
17 12
BstZ17I GTA'TAC
Cuts at: 2044 2044
Size: 5101
Bsu36I CC'TnA_GG
Cuts at: 2048 2048
Size: 5101
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4549
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4362
Fragments arranged by size:
4362 683 56
BtsI GCAGTG_nn'
Cuts at: 2617 3411 3431 2617
Size: 794 20 4287
Fragments arranged by size:
4287 794 20
ClaI AT'CG_AT
Cuts at: 1554 2361 1554
Size: 807 4294
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5066
DraI TTT'AAA
Cuts at: 2405 2405
Size: 5101
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2314 4108 809
Size: 507 998 1794 1802
Fragments arranged by size:
1802 1794 998 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2338 2548 3437
Size: 54 1425 50 275 18 508 210 889
Cuts at: 3437 5066 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 508 275 210 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2548 1537
Size: 275 736 4090
Fragments arranged by size:
4090 736 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3031 1353
Size: 1678 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3688 4062 4208 387
Size: 1064 138 2099 374 146 1280
Fragments arranged by size:
2099 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3164 3761 4548 4816 1239
Size: 393 1532 597 787 268 1524
Fragments arranged by size:
1532 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2410 2788 2410
Size: 378 4723
FspI TGC'GCA
Cuts at: 3607 3607
Size: 5101
HaeII r_GCGC'y
Cuts at: 1678 4248 1678
Size: 2570 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2052 2084 3742 3774 3816
Size: 32 135 32 480 32 1658 32 42
Cuts at: 3816 3848 1373
Size: 32 2626
Fragments arranged by size:
2626 1658 480 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2236 2445 2691 669
Size: 988 281 298 209 246 3079
Fragments arranged by size:
3079 988 298 281 246 209
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5101
HpaI GTT'AAC
Cuts at: 2691 2691
Size: 5101
KpnI G_GTAC'C
Cuts at: 2426 2426
Size: 5101
MfeI C'AATT_G
Cuts at: 2700 2700
Size: 5101
MluI A'CGCG_T
Cuts at: 4977 4977
Size: 5101
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4215 4399 1305
Size: 2910 184 2007
Fragments arranged by size:
2910 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3279
Fragments arranged by size:
3279 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2349 3059 3418 3577 518
Size: 648 1183 710 359 159 2042
Fragments arranged by size:
2042 1183 710 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2517 3194 4342 4587 1606
Size: 911 677 1148 245 2120
Fragments arranged by size:
2120 1148 911 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4549
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5101
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5101
NotI GC'GGCC_GC
Cuts at: 2548 2548
Size: 5101
NspI r_CATG'y
Cuts at: 1132 1229 2459 4004 1132
Size: 97 1230 1545 2229
Fragments arranged by size:
2229 1545 1230 97
PasI CC'CwG_GG
Cuts at: 1348 2107 1348
Size: 759 4342
PciI A'CATG_T
Cuts at: 4000 4000
Size: 5101
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2320 1108
Size: 1212 3889
PmeI GTTT'AAAC
Cuts at: 2405 2405
Size: 5101
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3170 3202 4683 4715 3170
Size: 32 1481 32 3556
Fragments arranged by size:
3556 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5101
PsiI TTA'TAA
Cuts at: 2671 2671
Size: 5101
PstI C_TGCA'G
Cuts at: 830 2453 830
Size: 1623 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5101
SalI G'TCGA_C
Cuts at: 1936 2443 1936
Size: 507 4594
SbfI CC_TGCA'GG
Cuts at: 2453 2453
Size: 5101
ScaI AGT'ACT
Cuts at: 3349 5061 3349
Size: 1712 3389
SfcI C'TryA_G
Cuts at: 826 1045 2449 3584 4265 4456 826
Size: 219 1404 1135 681 191 1471
Fragments arranged by size:
1471 1404 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5101
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5101
SmaI CCC'GGG
Cuts at: 1872 2428 1872
Size: 556 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2159 3205 4106 4368 820
Size: 197 926 216 1046 901 262 1553
Fragments arranged by size:
1553 1046 926 901 262 216 197
SnaBI TAC'GTA
Cuts at: 493 2892 493
Size: 2399 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5101
SphI G_CATG'C
Cuts at: 1132 2459 1132
Size: 1327 3774
SspI AAT'ATT
Cuts at: 5 52 3025 5
Size: 47 2973 2081
Fragments arranged by size:
2973 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5101
StyI C'CwwG_G
Cuts at: 513 1065 2540 513
Size: 552 1475 3074
Fragments arranged by size:
3074 1475 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3128 3145 3298 3483 3928 325
Size: 1255 1548 17 153 185 445 1498
Fragments arranged by size:
1548 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3347 5059 96
Size: 274 80 33 51 1443 1370 1712 138
Fragments arranged by size:
1712 1443 1370 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5061 5093 5061
Size: 32 5069
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5101
XbaI T'CTAG_A
Cuts at: 2437 2437
Size: 5101
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2083 2083
Size: 5101
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5101
XmaI C'CCGG_G
Cuts at: 1870 2426 1870
Size: 556 4545
XmnI GAAnn'nnTTC
Cuts at: 3230 3230
Size: 5101
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2909 276
Size: 53 83 186 936 1375 2468
Fragments arranged by size:
2468 1375 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI