################################## # NO CUT ENZYMES ( 196 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HphI KasI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MseI MspA1I MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AfaI 61 GTAC GT^AC AlwI 117 GGATC GGATC(4/5) AspHI 65 GAGCTC G[AT]GC[AT]^C BanII 65 GAGCTC G[GA]GC[CT]^C BbsI 91 GAAGAC GAAGAC(2/6) BbvI 212 GCAGC GCAGC(8/12) BfaI 25 CTAG C^TAG BglI 23 GCCTAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 65 GAGCTC G[AGT]GC[ACT]^C BpmI 207 CTGGAG CTGGAG(16/14) BpuAI 91 GAAGAC GAAGAC(2/6) BsiHKAI 65 GAGCTC G[AT]GC[AT]^C Bsp1286I 65 GAGCTC G[AGT]GC[ACT]^C BspHI 69 TCATGA T^CATGA BstF5I 225 GGATG GGATG(2/0) Cfr13I 147 GGCCC G^G[ACGT]CC DpnII 118 GATC ^GATC DsaI 221 CCATGG C^C[GA][CT]GG EarI 84 GAAGAG CTCTTC(1/4) EcoT14I 221 CCATGG C^C[AT][AT]GG Fnu4HI 212 GCAGC GC^[ACGT]GC FokI 225 GGATG GGATG(9/13) HapII 139 CCGG C^CGG HpaII 139 CCGG C^CGG ItaI 212 GCAGC GC^[ACGT]GC Ksp632I 84 GAAGAG CTCTTC(1/4) MaeI 25 CTAG C^TAG MaeIII 125 GTGAC ^GT[ACGT]AC MboI 118 GATC ^GATC MspI 139 CCGG C^CGG MwoI 23 GCCTAGTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 221 CCATGG C^CATGG NdeII 118 GATC ^GATC RcaI 69 TCATGA T^CATGA RsaI 61 GTAC GT^AC SacI 65 GAGCTC GAGCT^C SapI 84 GAAGAGC GCTCTTC(1/4) Sau3AI 118 GATC ^GATC Sau96I 147 GGCCC G^G[ACGT]CC StuI 200 AGGCCT AGG^CCT StyI 221 CCATGG C^C[AT][AT]GG TaqI 236 TCGA T^CGA TseI 212 GCAGC G^C[AT]GC Tsp45I 125 GTGAC ^GT[GC]AC Tsp509I 42 AATT ^AATT TspRI 182 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 236 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AluI 66 AGCT AG^CT 108 AGCT BsaJI 11 CCAGGG C^C[ACGT][ACGT]GG 221 CCATGG BsiYI 132 CCCCTTGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 216 CCTCTCCATGG BslI 132 CCCCTTGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 216 CCTCTCCATGG BssKI 11 CCAGG ^CC[ACGT]GG 206 CCTGG BstNI 11 CCAGG CC^[AT]GG 206 CCTGG EcoRII 11 CCAGG ^CC[AT]GG 206 CCTGG HaeIII 147 GGCC GG^CC 201 GGCC MboII 84 GAAGA GAAGA(8/7) 91 GAAGA MslI 48 CACCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 70 CATGAAAGTG MvaI 11 CCAGG CC^[AT]GG 206 CCTGG ScrFI 11 CCAGG CC^[ACGT]GG 206 CCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 3 enzymes) # ######################################### Cac8I MnlI NlaIII