################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BbeI BbrPI BbvCI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BseAI BseRI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AciI 474 GCGG CCGC(-3/-1) AgeI 172 ACCGGT A^CCGGT ApaI 708 GGGCCC GGGCC^C AspHI 592 GAGCAC G[AT]GC[AT]^C BamHI 253 GGATCC G^GATCC BanI 492 GGTGCC G^G[CT][GA]CC BcgI 353 GCAGCAGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 533 AGATCT A^GATCT BsaI 677 GGTCTC GGTCTC(1/5) BsaWI 172 ACCGGT [AT]^CCGG[AT] BsgI 413 GTGCAG GTGCAG(16/14) BsiHKAI 592 GAGCAC G[AT]GC[AT]^C BsrFI 172 ACCGGT [GA]^CCGG[CT] Cfr10I 172 ACCGGT [GA]^CCGG[CT] DdeI 447 CTTAG C^T[ACGT]AG EcoRI 178 GAATTC G^AATTC FauI 474 GCGGG CCCGC(4/6) HapII 173 CCGG C^CGG HgaI 39 GACGC GACGC(5/10) HincII 115 GTCAAC GT[CT]^[GA]AC HindII 115 GTCAAC GT[CT]^[GA]AC HpaII 173 CCGG C^CGG MaeIII 217 GTAAC ^GT[ACGT]AC MseI 343 TTAA T^TAA MslI 519 CATCCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 173 CCGG C^CGG PinAI 172 ACCGGT A^CCGGT PleI 359 GAGTC GAGTC(4/5) PstI 668 CTGCAG CTGCA^G SapI 501 GCTCTTC GCTCTTC(1/4) SfcI 668 CTGCAG C^T[GA][CT]AG TfiI 549 GAATC G^A[AT]TC Tru9I 343 TTAA T^TAA XcmI 392 CCAGACTTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcsI 178 GAATTC [GA]^AATT[CT] 283 AAATTT AfaI 99 GTAC GT^AC 444 GTAC ApoI 178 GAATTC [GA]^AATT[CT] 283 AAATTT AvaII 170 GGACC G^G[AT]CC 435 GGTCC BanII 479 GGGCTC G[GA]GC[CT]^C 708 GGGCCC BbsI 187 GAAGAC GAAGAC(2/6) 542 GTCTTC BpuAI 187 GAAGAC GAAGAC(2/6) 542 GTCTTC BsiYI 235 CCATTTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 471 CCTGCGGGGGG BslI 235 CCATTTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 471 CCTGCGGGGGG BsmAI 154 GAGAC GTCTC(1/5) 678 GTCTC BsmFI 436 GTCCC GGGAC(10/14) 719 GTCCC BspMI 470 ACCTGC ACCTGC(4/8) 415 GCAGGT BsrI 674 ACTGG ACTGG(1/-1) 90 CCAGT BssKI 54 CCTGG ^CC[ACGT]GG 261 CCTGG BstNI 54 CCTGG CC^[AT]GG 261 CCTGG BstXI 392 CCAGACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 531 CCAGATCTTTGG BstYI 253 GGATCC [GA]^GATC[CT] 533 AGATCT DraII 298 GGGGCCT [GA]G^G[ACGT]CC[CT] 707 GGGGCCC EarI 502 CTCTTC CTCTTC(1/4) 680 CTCTTC EcoO109I 298 GGGGCCT [GA]G^G[ACGT]CC[CT] 707 GGGGCCC EcoRII 54 CCTGG ^CC[AT]GG 261 CCTGG HinfI 359 GAGTC G^A[ACGT]TC 549 GAATC Ksp632I 502 CTCTTC CTCTTC(1/4) 680 CTCTTC MflI 253 GGATCC [GA]^GATC[CT] 533 AGATCT MvaI 54 CCTGG CC^[AT]GG 261 CCTGG RsaI 99 GTAC GT^AC 444 GTAC ScrFI 54 CCTGG CC^[ACGT]GG 261 CCTGG SfaNI 350 GATGC GCATC(5/9) 518 GCATC TspRI 128 CACTG CA[GC]TG[ACGT][ACGT]^ 510 CACTG XhoII 253 GGATCC [GA]^GATC[CT] 533 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AluI AlwI AvaI BbvI BmyI BsaJI BsoBI Bsp1286I BstF5I Cac8I Cfr13I DpnII EcoT14I Fnu4HI FokI HaeIII HphI ItaI MaeII MboI MboII MnlI NdeII NlaIII NlaIV Sau3AI Sau96I StyI TseI Tsp509I