(Linear) (Six-base) MAPSORT of: KIEE2476.seq Check: 705 from: 1 to: 1500 KIEE2476.orf With 182 enzymes: SgfI * June 18, 2013 11:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 591 805 1500 Size: 591 214 695 Fragments arranged by size: 695 591 214 AccI GT'mk_AC Cuts at: 0 977 1500 Size: 977 523 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 561 949 1078 1480 1500 Size: 561 388 129 402 20 Fragments arranged by size: 561 402 388 129 20 AflIII A'CryG_T Cuts at: 0 258 1500 Size: 258 1242 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 728 760 1500 Size: 728 32 740 Fragments arranged by size: 740 728 32 ApoI r'AATT_y Cuts at: 0 787 1149 1500 Size: 787 362 351 Fragments arranged by size: 787 362 351 AvaI C'yCGr_G Cuts at: 0 37 1206 1287 1500 Size: 37 1169 81 213 Fragments arranged by size: 1169 213 81 37 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 653 686 1500 Size: 653 33 814 Fragments arranged by size: 814 653 33 BanII G_rGCy'C Cuts at: 0 1213 1500 Size: 1213 287 BbsI GAAGACnn'nnnn_ Cuts at: 0 317 404 1066 1396 1406 1500 Size: 317 87 662 330 10 94 Fragments arranged by size: 662 330 317 94 87 10 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1268 1302 1500 Size: 1268 34 198 Fragments arranged by size: 1268 198 34 BclI T'GATC_A Cuts at: 0 891 1500 Size: 891 609 BglI GCCn_nnn'nGGC Cuts at: 0 97 1500 Size: 97 1403 Bme1580I G_kGCm'C Cuts at: 0 28 1500 Size: 28 1472 BsaWI w'CCGG_w Cuts at: 0 1180 1500 Size: 1180 320 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 728 758 1186 1216 1500 Size: 728 30 428 30 284 Fragments arranged by size: 728 428 284 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 568 1409 1500 Size: 568 841 91 Fragments arranged by size: 841 568 91 BseYI C'CCAG_C Cuts at: 0 763 1500 Size: 763 737 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 619 1500 Size: 34 585 881 Fragments arranged by size: 881 585 34 BsiHKAI G_wGCw'C Cuts at: 0 38 288 1500 Size: 38 250 1212 Fragments arranged by size: 1212 250 38 BsmI GAATG_Cn' Cuts at: 0 1412 1500 Size: 1412 88 Bsp1286I G_dGCh'C Cuts at: 0 28 38 288 1213 1500 Size: 28 10 250 925 287 Fragments arranged by size: 925 287 250 28 10 BspHI T'CATG_A Cuts at: 0 783 1500 Size: 783 717 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 591 805 1500 Size: 591 214 695 Fragments arranged by size: 695 591 214 BsrDI GCAATG_nn' Cuts at: 0 566 775 1500 Size: 566 209 725 Fragments arranged by size: 725 566 209 BsrFI r'CCGG_y Cuts at: 0 100 1500 Size: 100 1400 BstBI TT'CG_AA Cuts at: 0 198 1500 Size: 198 1302 BstXI CCAn_nnnn'nTGG Cuts at: 0 504 1500 Size: 504 996 BstYI r'GATC_y Cuts at: 0 321 1500 Size: 321 1179 Bsu36I CC'TnA_GG Cuts at: 0 419 1500 Size: 419 1081 BtgI C'CryG_G Cuts at: 0 626 690 1432 1500 Size: 626 64 742 68 Fragments arranged by size: 742 626 68 64 DraIII CAC_nnn'GTG Cuts at: 0 720 1500 Size: 720 780 EarI CTCTTCn'nnn_ Cuts at: 0 568 1500 Size: 568 932 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 561 949 1078 1480 1500 Size: 561 388 129 402 20 Fragments arranged by size: 561 402 388 129 20 EcoO109I rG'GnC_Cy Cuts at: 0 558 1351 1500 Size: 558 793 149 Fragments arranged by size: 793 558 149 EcoRI G'AATT_C Cuts at: 0 787 1500 Size: 787 713 FseI GG_CCGG'CC Cuts at: 0 104 1500 Size: 104 1396 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 391 423 1235 1267 1500 Size: 391 32 812 32 233 Fragments arranged by size: 812 391 233 32 32 HincII GTy'rAC Cuts at: 0 256 978 1173 1500 Size: 256 722 195 327 Fragments arranged by size: 722 327 256 195 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1178 1500 Size: 1178 322 MslI CAynn'nnrTG Cuts at: 0 152 220 293 1336 1500 Size: 152 68 73 1043 164 Fragments arranged by size: 1043 164 152 73 68 MspA1I CmG'CkG Cuts at: 0 763 1500 Size: 763 737 NaeI GCC'GGC Cuts at: 0 102 1500 Size: 102 1398 NgoMIV G'CCGG_C Cuts at: 0 100 1500 Size: 100 1400 NspI r_CATG'y Cuts at: 0 1235 1500 Size: 1235 265 PflMI CCAn_nnn'nTGG Cuts at: 0 592 1500 Size: 592 908 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 728 760 1500 Size: 728 32 740 Fragments arranged by size: 740 728 32 PpuMI rG'GwC_Cy Cuts at: 0 1351 1500 Size: 1351 149 PstI C_TGCA'G Cuts at: 0 111 1500 Size: 111 1389 PvuII CAG'CTG Cuts at: 0 763 1500 Size: 763 737 SalI G'TCGA_C Cuts at: 0 976 1500 Size: 976 524 SfcI C'TryA_G Cuts at: 0 107 235 1017 1500 Size: 107 128 782 483 Fragments arranged by size: 782 483 128 107 StyI C'CwwG_G Cuts at: 0 1256 1500 Size: 1256 244 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1190 1500 Size: 1190 310 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1360 1392 1500 Size: 1360 32 108 Fragments arranged by size: 1360 108 32 Tth111I GACn'n_nGTC Cuts at: 0 1264 1500 Size: 1264 236 Enzymes that do cut: AarI AccI AcuI AflIII AloI ApoI AvaI BaeI BanII BbsI BcgI BclI BglI Bme1580I BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BstBI BstXI BstYI Bsu36I BtgI DraIII EarI Eco57MI EcoO109I EcoRI FseI Hin4I HincII MmeI MslI MspA1I NaeI NgoMIV NspI PflMI PpiI PpuMI PstI PvuII SalI SfcI StyI TaqII TstI Tth111I Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BamHI BanI BbeI BbvCI BciVI BfrBI BglII BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BspEI BsrBI BsrGI BssHII BssSI BstAPI BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EciI EcoICRI EcoNI EcoRV FalI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MscI NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI XbaI XcmI XhoI XmaI XmnI ZraI