################################## # NO CUT ENZYMES ( 195 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BsiEI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AciI 28 GCGG CCGC(-3/-1) AlwI 489 GATCC GGATC(4/5) AseI 448 ATTAAT AT^TAAT AsnI 448 ATTAAT AT^TAAT AvaII 442 GGACC G^G[AT]CC BanII 212 GAGCTC G[GA]GC[CT]^C BciVI 41 GTATCC GTATCC(6/5) BpmI 163 CTGGAG CTGGAG(16/14) BseRI 209 GAGGAG GAGGAG(10/8) BsgI 245 GTGCAG GTGCAG(16/14) BsmAI 481 GAGAC GTCTC(1/5) BsmFI 441 GGGAC GGGAC(10/14) BsrDI 226 GCAATG GCAATG(2/0) BstF5I 415 GGATG GGATG(2/0) DraI 51 TTTAAA TTT^AAA DraII 441 GGGACCC [GA]G^G[ACGT]CC[CT] EaeI 95 CGGCCA [CT]^GGCC[GA] EcoO109I 441 GGGACCC [GA]G^G[ACGT]CC[CT] FokI 415 GGATG GGATG(9/13) HphI 202 GGTGA GGTGA(8/7) NlaIII 73 CATG CATG^ PpuMI 441 GGGACCC [GA]G^G[AT]CC[CT] PshBI 448 ATTAAT AT^TAAT SacI 212 GAGCTC GAGCT^C SapI 264 GAAGAGC GCTCTTC(1/4) SspI 451 AATATT AAT^ATT TfiI 510 GAATC G^A[AT]TC TspRI 432 CACTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AspHI 10 GTGCTC G[AT]GC[AT]^C 212 GAGCTC BbvI 465 GCAGC GCAGC(8/12) 334 GCTGC BmyI 10 GTGCTC G[AGT]GC[ACT]^C 212 GAGCTC BsiHKAI 10 GTGCTC G[AT]GC[AT]^C 212 GAGCTC Bsp1286I 10 GTGCTC G[AGT]GC[ACT]^C 212 GAGCTC BssKI 45 CCTGG ^CC[ACGT]GG 492 CCTGG BstNI 45 CCTGG CC^[AT]GG 492 CCTGG Cfr13I 3 GGCCC G^G[ACGT]CC 442 GGACC DpnII 400 GATC ^GATC 489 GATC EarI 264 GAAGAG CTCTTC(1/4) 436 GAAGAG EcoRII 45 CCTGG ^CC[AT]GG 492 CCTGG Fnu4HI 334 GCTGC GC^[ACGT]GC 465 GCAGC HaeIII 3 GGCC GG^CC 96 GGCC ItaI 334 GCTGC GC^[ACGT]GC 465 GCAGC Ksp632I 264 GAAGAG CTCTTC(1/4) 436 GAAGAG MboI 400 GATC ^GATC 489 GATC MboII 264 GAAGA GAAGA(8/7) 436 GAAGA MvaI 45 CCTGG CC^[AT]GG 492 CCTGG NdeII 400 GATC ^GATC 489 GATC PleI 22 GACTC GAGTC(4/5) 195 GACTC Sau3AI 400 GATC ^GATC 489 GATC Sau96I 3 GGCCC G^G[ACGT]CC 442 GGACC ScrFI 45 CCTGG CC^[ACGT]GG 492 CCTGG TaqI 35 TCGA T^CGA 60 TCGA TseI 334 GCTGC G^C[AT]GC 465 GCAGC TthHB8I 35 TCGA T^CGA 60 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### AluI DdeI HinfI MnlI MseI NlaIV SfaNI Tru9I Tsp509I