################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AcsI 445 AAATTT [GA]^AATT[CT] ApoI 445 AAATTT [GA]^AATT[CT] BamHI 141 GGATCC G^GATCC BanII 182 GAGCCC G[GA]GC[CT]^C BcnI 30 CCGGG CC^[GC]GG BmyI 182 GAGCCC G[AGT]GC[ACT]^C BsmFI 32 GGGAC GGGAC(10/14) Bsp1286I 182 GAGCCC G[AGT]GC[ACT]^C BspHI 591 TCATGA T^CATGA BstEII 267 GGTTACC G^GT[ACGT]ACC BstNI 659 CCAGG CC^[AT]GG BstPI 267 GGTTACC G^GT[ACGT]ACC Cac8I 510 GCAGGC GC[ACGT]^[ACGT]GC DraI 256 TTTAAA TTT^AAA DsaI 454 CCACGG C^C[GA][CT]GG EarI 429 GAAGAG CTCTTC(1/4) EcoO65I 267 GGTTACC G^GT[ACGT]ACC EcoRII 659 CCAGG ^CC[AT]GG EcoT14I 263 CCAAGG C^C[AT][AT]GG Ksp632I 429 GAAGAG CTCTTC(1/4) MvaI 659 CCAGG CC^[AT]GG NciI 30 CCGGG CC^[GC]GG PpuMI 32 GGGACCC [GA]G^G[AT]CC[CT] PvuII 629 CAGCTG CAG^CTG RcaI 591 TCATGA T^CATGA SfaNI 229 GATGC GCATC(5/9) StyI 263 CCAAGG C^C[AT][AT]GG SwaI 255 ATTTAAAT ATTT^AAAT TaqI 567 TCGA T^CGA TfiI 460 GATTC G^A[AT]TC Tsp45I 482 GTGAC ^GT[GC]AC TspRI 365 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 567 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AfaI 525 GTAC GT^AC 580 GTAC AflIII 326 ACGTGT A^C[GA][CT]GT 527 ACACGT AvaII 33 GGACC G^G[AT]CC 503 GGACC BsmAI 21 GAGAC GTCTC(1/5) 394 GAGAC BsmBI 21 GAGACG CGTCTC(1/5) 394 GAGACG BsrI 84 CCAGT ACTGG(1/-1) 621 CCAGT BssKI 30 CCGGG ^CC[ACGT]GG 659 CCAGG BstF5I 381 CATCC GGATG(2/0) 228 GGATG BstYI 141 GGATCC [GA]^GATC[CT] 557 GGATCT DdeI 114 CTCAG C^T[ACGT]AG 521 CTCAG DraII 32 GGGACCC [GA]G^G[ACGT]CC[CT] 512 AGGCCCC EcoO109I 32 GGGACCC [GA]G^G[ACGT]CC[CT] 512 AGGCCCC FokI 381 CATCC GGATG(9/13) 228 GGATG HaeIII 499 GGCC GG^CC 513 GGCC HapII 30 CCGG C^CGG 501 CCGG HpaII 30 CCGG C^CGG 501 CCGG MaeIII 268 GTTAC ^GT[ACGT]AC 482 GTGAC MboII 426 GAAGA GAAGA(8/7) 429 GAAGA MflI 141 GGATCC [GA]^GATC[CT] 557 GGATCT MseI 257 TTAA T^TAA 443 TTAA MspA1I 70 CAGCGG C[AC]G^C[GT]G 629 CAGCTG MspI 30 CCGG C^CGG 501 CCGG PleI 595 GACTC GAGTC(4/5) 155 GAGTC RsaI 525 GTAC GT^AC 580 GTAC ScrFI 30 CCGGG CC^[ACGT]GG 659 CCAGG Tru9I 257 TTAA T^TAA 443 TTAA Tsp509I 387 AATT ^AATT 446 AATT XhoII 141 GGATCC [GA]^GATC[CT] 557 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AciI AluI AlwI BbvI BsaJI BsiYI BslI Cfr13I DpnII FauI Fnu4HI HinfI ItaI MaeII MboI MnlI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I TseI