################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BanI BanII BbeI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiWI BsmBI BspDI BspEI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DsaI Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRV FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MluI MluNI MroI MscI MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI PacI PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I Van91I XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AciI 1905 CCGC CCGC(-3/-1) AlwNI 97 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 574 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1568 GAGCAC G[AT]GC[AT]^C AvaII 1054 GGTCC G^G[AT]CC BamHI 207 GGATCC G^GATCC BbrPI 526 CACGTG CAC^GTG BpmI 1508 CTCCAG CTGGAG(16/14) BsaAI 526 CACGTG [CT]AC^GT[GA] BsiEI 1902 CGGCCG CG[GA][CT]^CG BsiHKAI 1568 GAGCAC G[AT]GC[AT]^C Bsp1407I 295 TGTACA T^GTACA BspHI 396 TCATGA T^CATGA BsrGI 295 TGTACA T^GTACA BssKI 1689 CCAGG ^CC[ACGT]GG BstAPI 898 GCAAGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 375 TTCGAA TT^CGAA BstNI 1689 CCAGG CC^[AT]GG Cfr13I 1054 GGTCC G^G[ACGT]CC DraI 1528 TTTAAA TTT^AAA DrdI 1022 GACAATTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 1902 CGGCCG [CT]^GGCC[GA] EagI 1902 CGGCCG C^GGCCG EarI 1801 GAAGAG CTCTTC(1/4) EclXI 1902 CGGCCG C^GGCCG Eco52I 1902 CGGCCG C^GGCCG EcoRI 1409 GAATTC G^AATTC EcoRII 1689 CCAGG ^CC[AT]GG EcoT22I 251 ATGCAT ATGCA^T HphI 1997 TCACC GGTGA(8/7) Ksp632I 1801 GAAGAG CTCTTC(1/4) MslI 1594 CATGTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 460 CAGCTG C[AC]G^C[GT]G MvaI 1689 CCAGG CC^[AT]GG NlaIV 207 GGATCC GG[ACGT]^[ACGT]CC NsiI 251 ATGCAT ATGCA^T NspV 375 TTCGAA TT^CGAA PaeR7I 794 CTCGAG C^TCGAG PmaCI 526 CACGTG CAC^GTG PmlI 526 CACGTG CAC^GTG PvuII 460 CAGCTG CAG^CTG RcaI 396 TCATGA T^CATGA Sau96I 1054 GGTCC G^G[ACGT]CC ScrFI 1689 CCAGG CC^[ACGT]GG SfuI 375 TTCGAA TT^CGAA SspBI 295 TGTACA T^GTACA XbaI 1113 TCTAGA T^CTAGA XhoI 794 CTCGAG C^TCGAG XmnI 574 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 349 GTAGAC GT^[AC][GT]AC 557 GTCTAC AvaI 794 CTCGAG C^[CT]CG[GA]G 1538 CCCGAG BbsI 289 GAAGAC GAAGAC(2/6) 884 GTCTTC BmyI 721 GTGCCC G[AGT]GC[ACT]^C 1568 GAGCAC BpuAI 289 GAAGAC GAAGAC(2/6) 884 GTCTTC BsiYI 1750 CCCCAAGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 2085 CCAATGTGGGG BslI 1750 CCCCAAGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 2085 CCAATGTGGGG BsmAI 595 GAGAC GTCTC(1/5) 969 GAGAC BsoBI 794 CTCGAG C^[CT]CG[GA]G 1538 CCCGAG Bsp1286I 721 GTGCCC G[AGT]GC[ACT]^C 1568 GAGCAC BspMI 587 ACCTGC ACCTGC(4/8) 1785 GCAGGT BsrDI 154 GCAATG GCAATG(2/0) 704 GCAATG BsrI 943 ACTGG ACTGG(1/-1) 982 ACTGG BstYI 207 GGATCC [GA]^GATC[CT] 1434 AGATCC HaeIII 791 GGCC GG^CC 1903 GGCC MaeII 527 ACGT A^CGT 566 ACGT MfeI 1024 CAATTG C^AATTG 1727 CAATTG MflI 207 GGATCC [GA]^GATC[CT] 1434 AGATCC MunI 1024 CAATTG C^AATTG 1727 CAATTG PstI 94 CTGCAG CTGCA^G 457 CTGCAG XhoII 207 GGATCC [GA]^GATC[CT] 1434 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BbvI BfaI BsaJI BsmFI BsmI BstF5I DdeI DpnII EcoT14I Fnu4HI FokI HinfI ItaI MaeI MaeIII MboI MboII MnlI MseI MwoI NdeII NlaIII NspI PleI RsaI Sau3AI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I