(Circular) (Six-base) MAPSORT of: pFN21AE9210.seq Check: 5230 from: 1 to: 7114 pFN21AE9210 7114 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2376 2835 3471 4212 2376 Size: 459 636 741 5278 Fragments arranged by size: 5278 741 636 459 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4924 278 Size: 53 83 186 936 3388 2468 Fragments arranged by size: 3388 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2334 4457 1937 Size: 397 2123 4594 Fragments arranged by size: 4594 2123 397 Acc65I G'GTAC_C Cuts at: 4435 4435 Size: 7114 AclI AA'CG_TT Cuts at: 1563 5241 5614 1563 Size: 3678 373 3063 Fragments arranged by size: 3678 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3092 3641 5177 6561 6829 1632 Size: 1460 549 1536 1384 268 1917 Fragments arranged by size: 1917 1536 1460 1384 549 268 AfeI AGC'GCT Cuts at: 3359 3359 Size: 7114 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6917 AflIII A'CryG_T Cuts at: 1203 6013 6990 1203 Size: 4810 977 1327 Fragments arranged by size: 4810 1327 977 AgeI A'CCGG_T Cuts at: 5945 5945 Size: 7114 AhdI GACnn_n'nnGTC Cuts at: 5843 5843 Size: 7114 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7114 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7114 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2681 2713 2681 Size: 32 7082 AlwNI CAG_nnn'CTG Cuts at: 2668 2818 2959 3094 3250 3997 4857 6429 2668 Size: 150 141 135 156 747 860 1572 3353 Fragments arranged by size: 3353 1572 860 747 156 150 141 135 ApaI G_GGCC'C Cuts at: 2320 2395 2864 3113 3271 3752 2320 Size: 75 469 249 158 481 5682 Fragments arranged by size: 5682 481 469 249 158 75 ApaLI G'TGCA_C Cuts at: 2180 2822 5171 6327 2180 Size: 642 2349 1156 2967 Fragments arranged by size: 2967 2349 1156 642 ApoI r'AATT_y Cuts at: 1493 2558 4423 4649 4801 6910 1493 Size: 1065 1865 226 152 2109 1697 Fragments arranged by size: 2109 1865 1697 1065 226 152 AseI AT'TA_AT Cuts at: 160 1032 5668 160 Size: 872 4636 1606 Fragments arranged by size: 4636 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7114 AvaI C'yCGr_G Cuts at: 1870 1943 2394 4298 4439 1870 Size: 73 451 1904 141 4545 Fragments arranged by size: 4545 1904 451 141 73 BamHI G'GATC_C Cuts at: 4444 4811 4444 Size: 367 6747 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3368 3697 4200 4236 4273 Size: 325 217 627 1581 329 503 36 37 Cuts at: 4273 4435 5890 618 Size: 162 1455 1842 Fragments arranged by size: 1842 1581 1455 627 503 329 325 217 162 37 36 BanII G_rGCy'C Cuts at: 1391 2118 2320 2395 2756 2864 3113 3271 3752 Size: 727 202 75 361 108 249 158 481 Cuts at: 3752 4046 4286 4389 4433 1391 Size: 294 240 103 44 4072 Fragments arranged by size: 4072 727 481 361 294 249 240 202 158 108 103 75 44 BbeI G_GCGC'C Cuts at: 3372 3701 3372 Size: 329 6785 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2150 3824 4054 4109 928 Size: 971 251 1674 230 55 3933 Fragments arranged by size: 3933 1674 971 251 230 55 BbvCI CC'TCA_GC Cuts at: 2237 3806 2237 Size: 1569 5545 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5305 5339 1903 Size: 34 3368 34 3678 Fragments arranged by size: 3678 3368 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5006 6216 5006 Size: 1210 5904 BclI T'GATC_A Cuts at: 1548 1548 Size: 7114 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2585 3569 4368 Size: 107 122 71 631 605 913 984 799 Cuts at: 4368 5725 136 Size: 1357 1525 Fragments arranged by size: 1525 1357 984 913 799 631 605 122 107 71 BglII A'GATC_T Cuts at: 7109 7109 Size: 7114 BlpI GC'TnA_GC Cuts at: 3275 3284 4247 4531 3275 Size: 9 963 284 5858 Fragments arranged by size: 5858 963 284 9 Bme1580I G_kGCm'C Cuts at: 1790 2156 2184 2320 2395 2826 2864 3113 3271 Size: 366 28 136 75 431 38 249 158 Cuts at: 3271 3308 3752 4278 5175 6331 1790 Size: 37 444 526 897 1156 2573 Fragments arranged by size: 2573 1156 897 526 444 431 366 249 158 136 75 38 37 28 BmgBI CAC'GTC Cuts at: 1318 2920 3910 1318 Size: 1602 990 4522 Fragments arranged by size: 4522 1602 990 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2620 4845 5803 Size: 416 53 83 399 24 1203 2225 958 Cuts at: 5803 442 Size: 1753 Fragments arranged by size: 2225 1753 1203 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7114 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3057 3057 Size: 7114 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2444 2785 2786 3014 3251 4342 4421 5774 Size: 1205 341 1 228 237 1091 79 1353 Cuts at: 5774 1239 Size: 2579 Fragments arranged by size: 2579 1353 1205 1091 341 237 228 79 1 Bpu10I CC'TnA_GC Cuts at: 2237 3806 2237 Size: 1569 5545 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2921 4199 5239 6104 6402 2921 Size: 1278 1040 865 298 3633 Fragments arranged by size: 3633 1278 1040 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2501 3008 5777 882 Size: 617 1002 507 2769 2219 Fragments arranged by size: 2769 2219 1002 617 507 BsaAI yAC'GTr Cuts at: 493 1204 3148 4905 493 Size: 711 1944 1757 2702 Fragments arranged by size: 2702 1944 1757 711 BsaBI GATnn'nnATC Cuts at: 7108 7108 Size: 7114 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3102 3369 3483 3698 Size: 53 83 186 936 1569 267 114 215 Cuts at: 3698 3821 3936 4059 4921 5303 5938 275 Size: 123 115 123 862 382 635 1451 Fragments arranged by size: 1569 1451 936 862 635 382 267 215 186 123 123 115 114 83 53 BsaWI w'CCGG_w Cuts at: 3030 4031 5547 5945 6219 6366 6777 3030 Size: 1001 1516 398 274 147 411 3367 Fragments arranged by size: 3367 1516 1001 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2576 2606 2681 2711 2738 Size: 30 165 30 1173 30 75 30 27 Cuts at: 2738 2768 3128 3158 1178 Size: 30 360 30 5134 Fragments arranged by size: 5134 1173 360 165 75 30 30 30 30 30 30 27 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2183 2360 2702 2903 3149 3668 3754 1186 Size: 997 177 342 201 246 519 86 4546 Fragments arranged by size: 4546 997 519 342 246 201 177 86 BseYI C'CCAG_C Cuts at: 2848 3161 3372 3824 4039 4185 4207 6317 6813 Size: 313 211 452 215 146 22 2110 496 Cuts at: 6813 2848 Size: 3149 Fragments arranged by size: 3149 2110 496 452 313 215 211 146 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2372 2699 3080 3185 1163 Size: 573 636 327 381 105 5092 Fragments arranged by size: 5092 636 573 381 327 105 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2517 4368 4564 5325 6353 1540 Size: 275 186 516 1851 196 761 1028 2301 Fragments arranged by size: 2301 1851 1028 761 516 275 196 186 BsiHKAI G_wGCw'C Cuts at: 2184 2826 3194 5175 5260 6331 2184 Size: 642 368 1981 85 1071 2967 Fragments arranged by size: 2967 1981 1071 642 368 85 BsmI GAATG_Cn' Cuts at: 4625 4718 4625 Size: 93 7021 BsmBI CGTCTCn'nnnn_ Cuts at: 2543 2780 2543 Size: 237 6877 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2156 2184 2320 2395 2756 2826 Size: 399 328 38 28 136 75 361 70 Cuts at: 2826 2864 3113 3194 3271 3308 3752 4046 4278 Size: 38 249 81 77 37 444 294 232 Cuts at: 4278 4286 4389 4433 5175 5260 6331 1391 Size: 8 103 44 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 444 399 361 328 294 249 232 136 103 85 81 77 75 70 44 38 38 37 28 8 BspEI T'CCGG_A Cuts at: 3030 6777 3030 Size: 3747 3367 BspHI T'CATG_A Cuts at: 5003 6733 5003 Size: 1730 5384 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2376 2835 2940 2988 3102 3471 3681 3907 Size: 1532 459 105 48 114 369 210 226 Cuts at: 3907 3969 4212 4469 844 Size: 62 243 257 3489 Fragments arranged by size: 3489 1532 459 369 257 243 226 210 114 105 62 48 BsrBI CCG'CTC Cuts at: 1394 1823 3291 4251 5001 1394 Size: 429 1468 960 750 3507 Fragments arranged by size: 3507 1468 960 750 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2461 5609 5783 65 Size: 1183 1213 3148 174 1396 Fragments arranged by size: 3148 1396 1213 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2403 2727 3550 3938 4197 4367 5758 Size: 110 593 324 823 388 259 170 1391 Cuts at: 5758 5945 1700 Size: 187 2869 Fragments arranged by size: 2869 1391 823 593 388 324 259 187 170 110 BsrGI T'GTAC_A Cuts at: 96 2025 96 Size: 1929 5185 BssHII G'CGCG_C Cuts at: 1925 3240 3253 3279 3319 1925 Size: 1315 13 26 40 5720 Fragments arranged by size: 5720 1315 40 26 13 BssSI C'ACGA_G Cuts at: 2837 3377 3641 4279 5174 6186 2837 Size: 540 264 638 895 1012 3765 Fragments arranged by size: 3765 1012 895 638 540 264 BstAPI GCAn_nnn'nTGC Cuts at: 794 3094 3250 3913 794 Size: 2300 156 663 3995 Fragments arranged by size: 3995 2300 663 156 BstBI TT'CG_AA Cuts at: 5918 5918 Size: 7114 BstEII G'GTnAC_C Cuts at: 1184 4138 1184 Size: 2954 4160 BstXI CCAn_nnnn'nTGG Cuts at: 2437 3169 3643 2437 Size: 732 474 5908 Fragments arranged by size: 5908 732 474 BstYI r'GATC_y Cuts at: 1972 2361 2434 4444 4811 5196 5213 6665 6751 Size: 389 73 2010 367 385 17 1452 86 Cuts at: 6751 6763 7109 1972 Size: 12 346 1977 Fragments arranged by size: 2010 1977 1452 389 385 367 346 86 73 17 12 Bsu36I CC'TnA_GG Cuts at: 2298 2298 Size: 7114 BtgI C'CryG_G Cuts at: 513 1065 2004 2580 3057 3312 4066 513 Size: 552 939 576 477 255 754 3561 Fragments arranged by size: 3561 939 754 576 552 477 255 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3379 487 Size: 683 56 2153 4222 Fragments arranged by size: 4222 2153 683 56 BtsI GCAGTG_nn' Cuts at: 3971 4630 5424 5444 3971 Size: 659 794 20 5641 Fragments arranged by size: 5641 794 659 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7114 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3039 3074 529 Size: 35 2475 35 4569 Fragments arranged by size: 4569 2475 35 35 DraI TTT'AAA Cuts at: 4418 4418 Size: 7114 DraIII CAC_nnn'GTG Cuts at: 2139 2206 2907 2139 Size: 67 701 6346 Fragments arranged by size: 6346 701 67 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2474 6121 809 Size: 507 1158 3647 1802 Fragments arranged by size: 3647 1802 1158 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2384 2922 Size: 54 1425 50 275 18 177 377 538 Cuts at: 2922 2961 3078 3513 4365 4561 5450 7079 8 Size: 39 117 435 852 196 889 1629 43 Fragments arranged by size: 1629 1425 889 852 538 435 377 275 196 177 117 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 4365 4561 1537 Size: 275 2553 196 4090 Fragments arranged by size: 4090 2553 275 196 EarI CTCTTCn'nnn_ Cuts at: 1353 2948 2990 3344 3461 3728 5044 1353 Size: 1595 42 354 117 267 1316 3423 Fragments arranged by size: 3423 1595 1316 354 267 117 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3320 5701 6075 6221 387 Size: 1064 138 1731 2381 374 146 1280 Fragments arranged by size: 2381 1731 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2444 2785 2786 3014 3092 3251 3641 Size: 393 812 341 1 228 78 159 390 Cuts at: 3641 4342 4421 5177 5774 6561 6829 1239 Size: 701 79 756 597 787 268 1524 Fragments arranged by size: 1524 812 787 756 701 597 393 390 341 268 228 159 79 78 1 EcoNI CCTnn'n_nnAGG Cuts at: 2327 2951 4343 2327 Size: 624 1392 5098 Fragments arranged by size: 5098 1392 624 EcoO109I rG'GnC_Cy Cuts at: 2391 2614 2860 3110 3267 3268 3748 3749 3802 Size: 223 246 250 157 1 480 1 53 Cuts at: 3802 3945 4336 2391 Size: 143 391 5169 Fragments arranged by size: 5169 480 391 250 246 223 157 143 53 1 1 EcoRI G'AATT_C Cuts at: 4423 4801 4423 Size: 378 6736 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3799 3799 Size: 7114 FspI TGC'GCA Cuts at: 5620 5620 Size: 7114 HaeII r_GCGC'y Cuts at: 1678 2591 3361 3372 3475 3701 3843 6261 1678 Size: 913 770 11 103 226 142 2418 2531 Fragments arranged by size: 2531 2418 913 770 226 142 103 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5755 5787 5829 5861 1373 Size: 32 135 32 4183 32 42 32 2626 Fragments arranged by size: 4183 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2335 4458 4704 669 Size: 988 281 397 2123 246 3079 Fragments arranged by size: 3079 2123 988 397 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7114 HpaI GTT'AAC Cuts at: 4704 4704 Size: 7114 KasI G'GCGC_C Cuts at: 3368 3697 3368 Size: 329 6785 KpnI G_GTAC'C Cuts at: 4439 4439 Size: 7114 MfeI C'AATT_G Cuts at: 4713 4713 Size: 7114 MluI A'CGCG_T Cuts at: 6990 6990 Size: 7114 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6228 6412 1305 Size: 4923 184 2007 Fragments arranged by size: 4923 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2386 2924 2963 3515 Size: 54 1425 343 177 377 538 39 552 Cuts at: 3515 10 Size: 3609 Fragments arranged by size: 3609 1425 552 538 377 343 177 54 39 MslI CAynn'nnrTG Cuts at: 518 1166 3381 5072 5431 5590 518 Size: 648 2215 1691 359 159 2042 Fragments arranged by size: 2215 2042 1691 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2310 2764 2872 2992 3586 3619 3679 3872 Size: 704 454 108 120 594 33 60 193 Cuts at: 3872 4530 5207 6355 6600 1606 Size: 658 677 1148 245 2120 Fragments arranged by size: 2120 1148 704 677 658 594 454 245 193 120 108 60 33 NaeI GCC'GGC Cuts at: 2405 2729 3552 3940 4369 2405 Size: 324 823 388 429 5150 Fragments arranged by size: 5150 823 429 388 324 NarI GG'CG_CC Cuts at: 3369 3698 3369 Size: 329 6785 NcoI C'CATG_G Cuts at: 513 1065 2004 2580 513 Size: 552 939 576 5047 Fragments arranged by size: 5047 939 576 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7114 NgoMIV G'CCGG_C Cuts at: 2403 2727 3550 3938 4367 2403 Size: 324 823 388 429 5150 Fragments arranged by size: 5150 823 429 388 324 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7114 NotI GC'GGCC_GC Cuts at: 4561 4561 Size: 7114 NruI TCG'CGA Cuts at: 3815 3815 Size: 7114 NspI r_CATG'y Cuts at: 1132 1229 2857 4472 6017 1132 Size: 97 1628 1615 1545 2229 Fragments arranged by size: 2229 1628 1615 1545 97 PasI CC'CwG_GG Cuts at: 1348 2864 1348 Size: 1516 5598 PciI A'CATG_T Cuts at: 6013 6013 Size: 7114 PflMI CCAn_nnn'nTGG Cuts at: 1108 2139 2206 2818 2920 3532 3610 4227 1108 Size: 1031 67 612 102 612 78 617 3995 Fragments arranged by size: 3995 1031 617 612 612 102 78 67 PfoI T'CCnGG_A Cuts at: 2973 4398 2973 Size: 1425 5689 PmeI GTTT'AAAC Cuts at: 4418 4418 Size: 7114 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2681 2713 5183 5215 6696 6728 2681 Size: 32 2470 32 1481 32 3067 Fragments arranged by size: 3067 2470 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2614 4336 2614 Size: 1722 5392 PshAI GACnn'nnGTC Cuts at: 1613 2330 1613 Size: 717 6397 PsiI TTA'TAA Cuts at: 4684 4684 Size: 7114 PspOMI G'GGCC_C Cuts at: 2316 2391 2860 3109 3267 3748 2316 Size: 75 469 249 158 481 5682 Fragments arranged by size: 5682 481 469 249 158 75 PstI C_TGCA'G Cuts at: 830 2571 2709 3591 3678 3966 4149 4335 4410 Size: 1741 138 882 87 288 183 186 75 Cuts at: 4410 4466 830 Size: 56 3478 Fragments arranged by size: 3478 1741 882 288 186 183 138 87 75 56 PvuI CG_AT'CG Cuts at: 2001 2517 2001 Size: 516 6598 PvuII CAG'CTG Cuts at: 2764 2872 2992 3619 2764 Size: 108 120 627 6259 Fragments arranged by size: 6259 627 120 108 SalI G'TCGA_C Cuts at: 1936 2333 4456 1936 Size: 397 2123 4594 Fragments arranged by size: 4594 2123 397 SapI GCTCTTCn'nnn_ Cuts at: 2948 2990 3344 3461 2948 Size: 42 354 117 6601 Fragments arranged by size: 6601 354 117 42 SbfI CC_TGCA'GG Cuts at: 4149 4466 4149 Size: 317 6797 ScaI AGT'ACT Cuts at: 5362 7074 5362 Size: 1712 5402 SfcI C'TryA_G Cuts at: 826 1045 2567 2705 3587 3674 3962 4145 4331 Size: 219 1522 138 882 87 288 183 186 Cuts at: 4331 4406 4462 5597 6278 6469 826 Size: 75 56 1135 681 191 1471 Fragments arranged by size: 1522 1471 1135 882 681 288 219 191 186 183 138 87 75 56 SfoI GGC'GCC Cuts at: 3370 3699 3370 Size: 329 6785 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7114 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7114 SmaI CCC'GGG Cuts at: 1872 2396 4441 1872 Size: 524 2045 4545 Fragments arranged by size: 4545 2045 524 SmlI C'TyrA_G Cuts at: 820 1017 1943 2936 4178 5218 6119 6381 820 Size: 197 926 993 1242 1040 901 262 1553 Fragments arranged by size: 1553 1242 1040 993 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4905 493 Size: 4412 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7114 SphI G_CATG'C Cuts at: 1132 2857 4472 1132 Size: 1725 1615 3774 Fragments arranged by size: 3774 1725 1615 SrfI GCCC'GGGC Cuts at: 2396 2396 Size: 7114 SspI AAT'ATT Cuts at: 5 52 5038 5 Size: 47 4986 2081 Fragments arranged by size: 4986 2081 47 StuI AGG'CCT Cuts at: 1517 2603 1517 Size: 1086 6028 StyI C'CwwG_G Cuts at: 513 1065 2004 2061 2580 2964 3516 3564 3738 Size: 552 939 57 519 384 552 48 174 Cuts at: 3738 4553 513 Size: 815 3074 Fragments arranged by size: 3074 939 815 552 552 519 384 174 57 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3230 5141 5158 5311 5496 5941 325 Size: 1255 1650 1911 17 153 185 445 1498 Fragments arranged by size: 1911 1650 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2025 5360 7072 Size: 274 80 33 51 1443 48 3335 1712 Cuts at: 7072 96 Size: 138 Fragments arranged by size: 3335 1712 1443 274 138 80 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2576 2608 3173 3205 3711 3743 7074 7106 2576 Size: 32 565 32 506 32 3331 32 2584 Fragments arranged by size: 3331 2584 565 506 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2618 4129 1316 Size: 1302 1511 4301 Fragments arranged by size: 4301 1511 1302 XbaI T'CTAG_A Cuts at: 4450 4450 Size: 7114 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2971 4396 2971 Size: 1425 5689 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7114 XmaI C'CCGG_G Cuts at: 1870 2394 4439 1870 Size: 524 2045 4545 Fragments arranged by size: 4545 2045 524 XmnI GAAnn'nnTTC Cuts at: 3626 5243 3626 Size: 1617 5497 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4922 276 Size: 53 83 186 936 3388 2468 Fragments arranged by size: 3388 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoICRI EcoRV FseI FspAI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SwaI