(Linear) (Six-base) MAPSORT of: KIEE9210.seq Check: 6865 from: 1 to: 2409 KIEE9210.orf With 182 enzymes: SgfI * July 3, 2012 19:01 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 371 830 1466 2207 2409 Size: 371 459 636 741 202 Fragments arranged by size: 741 636 459 371 202 AccI GT'mk_AC Cuts at: 0 329 2409 Size: 329 2080 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1087 1636 2409 Size: 1087 549 773 Fragments arranged by size: 1087 773 549 AfeI AGC'GCT Cuts at: 0 1354 2409 Size: 1354 1055 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 676 708 2409 Size: 676 32 1701 Fragments arranged by size: 1701 676 32 AlwNI CAG_nnn'CTG Cuts at: 0 663 813 954 1089 1245 1992 2409 Size: 663 150 141 135 156 747 417 Fragments arranged by size: 747 663 417 156 150 141 135 ApaI G_GGCC'C Cuts at: 0 315 390 859 1108 1266 1747 2409 Size: 315 75 469 249 158 481 662 Fragments arranged by size: 662 481 469 315 249 158 75 ApaLI G'TGCA_C Cuts at: 0 175 817 2409 Size: 175 642 1592 Fragments arranged by size: 1592 642 175 ApoI r'AATT_y Cuts at: 0 553 2409 Size: 553 1856 AvaI C'yCGr_G Cuts at: 0 389 2293 2409 Size: 389 1904 116 Fragments arranged by size: 1904 389 116 BanI G'GyrC_C Cuts at: 0 1363 1692 2195 2231 2268 2409 Size: 1363 329 503 36 37 141 Fragments arranged by size: 1363 503 329 141 37 36 BanII G_rGCy'C Cuts at: 0 113 315 390 751 859 1108 1266 1747 Size: 113 202 75 361 108 249 158 481 Cuts at: 1747 2041 2281 2384 2409 Size: 294 240 103 25 Fragments arranged by size: 481 361 294 249 240 202 158 113 108 103 75 25 BbeI G_GCGC'C Cuts at: 0 1367 1696 2409 Size: 1367 329 713 Fragments arranged by size: 1367 713 329 BbsI GAAGACnn'nnnn_ Cuts at: 0 145 1819 2049 2104 2409 Size: 145 1674 230 55 305 Fragments arranged by size: 1674 305 230 145 55 BbvCI CC'TCA_GC Cuts at: 0 232 1801 2409 Size: 232 1569 608 Fragments arranged by size: 1569 608 232 BglI GCCn_nnn'nGGC Cuts at: 0 580 1564 2363 2409 Size: 580 984 799 46 Fragments arranged by size: 984 799 580 46 BlpI GC'TnA_GC Cuts at: 0 1270 1279 2242 2409 Size: 1270 9 963 167 Fragments arranged by size: 1270 963 167 9 Bme1580I G_kGCm'C Cuts at: 0 151 179 315 390 821 859 1108 1266 Size: 151 28 136 75 431 38 249 158 Cuts at: 1266 1303 1747 2273 2409 Size: 37 444 526 136 Fragments arranged by size: 526 444 431 249 158 151 136 136 75 38 37 28 BmgBI CAC'GTC Cuts at: 0 915 1905 2409 Size: 915 990 504 Fragments arranged by size: 990 915 504 BmrI ACTGGGnnnn_n' Cuts at: 0 615 2409 Size: 615 1794 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1052 2409 Size: 1052 1357 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 439 780 781 1009 1246 2337 2409 Size: 439 341 1 228 237 1091 72 Fragments arranged by size: 1091 439 341 237 228 72 1 Bpu10I CC'TnA_GC Cuts at: 0 232 1801 2409 Size: 232 1569 608 Fragments arranged by size: 1569 608 232 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 916 2194 2409 Size: 916 1278 215 Fragments arranged by size: 1278 916 215 BsaI GGTCTCn'nnnn_ Cuts at: 0 496 1003 2409 Size: 496 507 1406 Fragments arranged by size: 1406 507 496 BsaAI yAC'GTr Cuts at: 0 1143 2409 Size: 1143 1266 BsaHI Gr'CG_yC Cuts at: 0 1097 1364 1478 1693 1816 1931 2054 2409 Size: 1097 267 114 215 123 115 123 355 Fragments arranged by size: 1097 355 267 215 123 123 115 114 BsaWI w'CCGG_w Cuts at: 0 1025 2026 2409 Size: 1025 1001 383 Fragments arranged by size: 1025 1001 383 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 571 601 676 706 733 763 1123 1153 Size: 571 30 75 30 27 30 360 30 Cuts at: 1153 2409 Size: 1256 Fragments arranged by size: 1256 571 360 75 30 30 30 30 27 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 178 355 697 898 1144 1663 1749 2409 Size: 178 177 342 201 246 519 86 660 Fragments arranged by size: 660 519 342 246 201 178 177 86 BseYI C'CCAG_C Cuts at: 0 843 1156 1367 1819 2034 2180 2202 2409 Size: 843 313 211 452 215 146 22 207 Fragments arranged by size: 843 452 313 215 211 207 146 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 367 694 1075 1180 2409 Size: 367 327 381 105 1229 Fragments arranged by size: 1229 381 367 327 105 BsiEI CG_ry'CG Cuts at: 0 512 2363 2409 Size: 512 1851 46 Fragments arranged by size: 1851 512 46 BsiHKAI G_wGCw'C Cuts at: 0 179 821 1189 2409 Size: 179 642 368 1220 Fragments arranged by size: 1220 642 368 179 BsmBI CGTCTCn'nnnn_ Cuts at: 0 538 775 2409 Size: 538 237 1634 Fragments arranged by size: 1634 538 237 Bsp1286I G_dGCh'C Cuts at: 0 113 151 179 315 390 751 821 859 Size: 113 38 28 136 75 361 70 38 Cuts at: 859 1108 1189 1266 1303 1747 2041 2273 2281 Size: 249 81 77 37 444 294 232 8 Cuts at: 2281 2384 2409 Size: 103 25 Fragments arranged by size: 444 361 294 249 232 136 113 103 81 77 75 70 38 38 37 28 25 8 BspEI T'CCGG_A Cuts at: 0 1025 2409 Size: 1025 1384 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 371 830 935 983 1097 1466 1676 1902 Size: 371 459 105 48 114 369 210 226 Cuts at: 1902 1964 2207 2409 Size: 62 243 202 Fragments arranged by size: 459 371 369 243 226 210 202 114 105 62 48 BsrBI CCG'CTC Cuts at: 0 1286 2246 2409 Size: 1286 960 163 Fragments arranged by size: 1286 960 163 BsrDI GCAATG_nn' Cuts at: 0 456 2409 Size: 456 1953 BsrFI r'CCGG_y Cuts at: 0 398 722 1545 1933 2192 2362 2409 Size: 398 324 823 388 259 170 47 Fragments arranged by size: 823 398 388 324 259 170 47 BsrGI T'GTAC_A Cuts at: 0 20 2409 Size: 20 2389 BssHII G'CGCG_C Cuts at: 0 1235 1248 1274 1314 2409 Size: 1235 13 26 40 1095 Fragments arranged by size: 1235 1095 40 26 13 BssSI C'ACGA_G Cuts at: 0 832 1372 1636 2274 2409 Size: 832 540 264 638 135 Fragments arranged by size: 832 638 540 264 135 BstAPI GCAn_nnn'nTGC Cuts at: 0 1089 1245 1908 2409 Size: 1089 156 663 501 Fragments arranged by size: 1089 663 501 156 BstEII G'GTnAC_C Cuts at: 0 2133 2409 Size: 2133 276 BstXI CCAn_nnnn'nTGG Cuts at: 0 432 1164 1638 2409 Size: 432 732 474 771 Fragments arranged by size: 771 732 474 432 BstYI r'GATC_y Cuts at: 0 356 429 2409 Size: 356 73 1980 Fragments arranged by size: 1980 356 73 Bsu36I CC'TnA_GG Cuts at: 0 293 2409 Size: 293 2116 BtgI C'CryG_G Cuts at: 0 575 1052 1307 2061 2409 Size: 575 477 255 754 348 Fragments arranged by size: 754 575 477 348 255 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1374 2409 Size: 1374 1035 BtsI GCAGTG_nn' Cuts at: 0 1966 2409 Size: 1966 443 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1034 1069 2409 Size: 1034 35 1340 Fragments arranged by size: 1340 1034 35 DraIII CAC_nnn'GTG Cuts at: 0 134 201 902 2409 Size: 134 67 701 1507 Fragments arranged by size: 1507 701 134 67 DrdI GACnn_nn'nnGTC Cuts at: 0 469 2409 Size: 469 1940 EaeI y'GGCC_r Cuts at: 0 2 379 917 956 1073 1508 2360 2409 Size: 2 377 538 39 117 435 852 49 Fragments arranged by size: 852 538 435 377 117 49 39 2 EagI C'GGCC_G Cuts at: 0 2360 2409 Size: 2360 49 EarI CTCTTCn'nnn_ Cuts at: 0 943 985 1339 1456 1723 2409 Size: 943 42 354 117 267 686 Fragments arranged by size: 943 686 354 267 117 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1315 2409 Size: 1315 1094 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 439 780 781 1009 1087 1246 1636 2337 Size: 439 341 1 228 78 159 390 701 Cuts at: 2337 2409 Size: 72 Fragments arranged by size: 701 439 390 341 228 159 78 72 1 EcoNI CCTnn'n_nnAGG Cuts at: 0 322 946 2338 2409 Size: 322 624 1392 71 Fragments arranged by size: 1392 624 322 71 EcoO109I rG'GnC_Cy Cuts at: 0 386 609 855 1105 1262 1263 1743 1744 Size: 386 223 246 250 157 1 480 1 Cuts at: 1744 1797 1940 2331 2409 Size: 53 143 391 78 Fragments arranged by size: 480 391 386 250 246 223 157 143 78 53 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1794 2409 Size: 1794 615 HaeII r_GCGC'y Cuts at: 0 586 1356 1367 1470 1696 1838 2409 Size: 586 770 11 103 226 142 571 Fragments arranged by size: 770 586 571 226 142 103 11 HincII GTy'rAC Cuts at: 0 330 2409 Size: 330 2079 KasI G'GCGC_C Cuts at: 0 1363 1692 2409 Size: 1363 329 717 Fragments arranged by size: 1363 717 329 MscI TGG'CCA Cuts at: 0 4 381 919 958 1510 2409 Size: 4 377 538 39 552 899 Fragments arranged by size: 899 552 538 377 39 4 MslI CAynn'nnrTG Cuts at: 0 1376 2409 Size: 1376 1033 MspA1I CmG'CkG Cuts at: 0 305 759 867 987 1581 1614 1674 1867 Size: 305 454 108 120 594 33 60 193 Cuts at: 1867 2409 Size: 542 Fragments arranged by size: 594 542 454 305 193 120 108 60 33 NaeI GCC'GGC Cuts at: 0 400 724 1547 1935 2364 2409 Size: 400 324 823 388 429 45 Fragments arranged by size: 823 429 400 388 324 45 NarI GG'CG_CC Cuts at: 0 1364 1693 2409 Size: 1364 329 716 Fragments arranged by size: 1364 716 329 NcoI C'CATG_G Cuts at: 0 575 2409 Size: 575 1834 NgoMIV G'CCGG_C Cuts at: 0 398 722 1545 1933 2362 2409 Size: 398 324 823 388 429 47 Fragments arranged by size: 823 429 398 388 324 47 NruI TCG'CGA Cuts at: 0 1810 2409 Size: 1810 599 NspI r_CATG'y Cuts at: 0 852 2409 Size: 852 1557 PasI CC'CwG_GG Cuts at: 0 859 2409 Size: 859 1550 PflMI CCAn_nnn'nTGG Cuts at: 0 134 201 813 915 1527 1605 2222 2409 Size: 134 67 612 102 612 78 617 187 Fragments arranged by size: 617 612 612 187 134 102 78 67 PfoI T'CCnGG_A Cuts at: 0 968 2393 2409 Size: 968 1425 16 Fragments arranged by size: 1425 968 16 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 676 708 2409 Size: 676 32 1701 Fragments arranged by size: 1701 676 32 PpuMI rG'GwC_Cy Cuts at: 0 609 2331 2409 Size: 609 1722 78 Fragments arranged by size: 1722 609 78 PshAI GACnn'nnGTC Cuts at: 0 325 2409 Size: 325 2084 PspOMI G'GGCC_C Cuts at: 0 311 386 855 1104 1262 1743 2409 Size: 311 75 469 249 158 481 666 Fragments arranged by size: 666 481 469 311 249 158 75 PstI C_TGCA'G Cuts at: 0 566 704 1586 1673 1961 2144 2330 2405 Size: 566 138 882 87 288 183 186 75 Cuts at: 2405 2409 Size: 4 Fragments arranged by size: 882 566 288 186 183 138 87 75 4 PvuI CG_AT'CG Cuts at: 0 512 2409 Size: 512 1897 PvuII CAG'CTG Cuts at: 0 759 867 987 1614 2409 Size: 759 108 120 627 795 Fragments arranged by size: 795 759 627 120 108 SalI G'TCGA_C Cuts at: 0 328 2409 Size: 328 2081 SapI GCTCTTCn'nnn_ Cuts at: 0 943 985 1339 1456 2409 Size: 943 42 354 117 953 Fragments arranged by size: 953 943 354 117 42 SbfI CC_TGCA'GG Cuts at: 0 2144 2409 Size: 2144 265 SfcI C'TryA_G Cuts at: 0 562 700 1582 1669 1957 2140 2326 2401 Size: 562 138 882 87 288 183 186 75 Cuts at: 2401 2409 Size: 8 Fragments arranged by size: 882 562 288 186 183 138 87 75 8 SfoI GGC'GCC Cuts at: 0 1365 1694 2409 Size: 1365 329 715 Fragments arranged by size: 1365 715 329 SmaI CCC'GGG Cuts at: 0 391 2409 Size: 391 2018 SmlI C'TyrA_G Cuts at: 0 931 2173 2409 Size: 931 1242 236 Fragments arranged by size: 1242 931 236 SphI G_CATG'C Cuts at: 0 852 2409 Size: 852 1557 SrfI GCCC'GGGC Cuts at: 0 391 2409 Size: 391 2018 StuI AGG'CCT Cuts at: 0 598 2409 Size: 598 1811 StyI C'CwwG_G Cuts at: 0 56 575 959 1511 1559 1733 2409 Size: 56 519 384 552 48 174 676 Fragments arranged by size: 676 552 519 384 174 56 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1225 2409 Size: 1225 1184 TatI w'GTAC_w Cuts at: 0 20 2409 Size: 20 2389 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 571 603 1168 1200 1706 1738 2409 Size: 571 32 565 32 506 32 671 Fragments arranged by size: 671 571 565 506 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 613 2124 2409 Size: 613 1511 285 Fragments arranged by size: 1511 613 285 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 966 2391 2409 Size: 966 1425 18 Fragments arranged by size: 1425 966 18 XmaI C'CCGG_G Cuts at: 0 389 2409 Size: 389 2020 XmnI GAAnn'nnTTC Cuts at: 0 1621 2409 Size: 1621 788 Enzymes that do cut: AarI AccI AcuI AfeI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII HincII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI PflMI PfoI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII SalI SapI SbfI SfcI SfoI SmaI SmlI SphI SrfI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglII BmtI BsaBI BsiWI BsmI BspHI BstBI BstZ17I ClaI DraI EcoICRI EcoRI EcoRV FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NotI NsiI PacI PciI PmeI PmlI PsiI PspXI PsrI RsrII SacI SacII SanDI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SspI SwaI XbaI XhoI ZraI