(Circular) (Six-base) MAPSORT of: pFN21AE9444.seq Check: 5986 from: 1 to: 9112 pFN21AE9444 9112 bp With 182 enzymes: SgfI * July 6, 2012 17:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2154 6922 278 Size: 53 83 186 936 618 4768 2468 Fragments arranged by size: 4768 2468 936 618 186 83 53 AccI GT'mk_AC Cuts at: 1937 2430 5007 6006 6455 1937 Size: 493 2577 999 449 4594 Fragments arranged by size: 4594 2577 999 493 449 Acc65I G'GTAC_C Cuts at: 6433 6433 Size: 9112 AclI AA'CG_TT Cuts at: 1563 7239 7612 1563 Size: 5676 373 3063 Fragments arranged by size: 5676 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3860 3926 5804 7175 8559 8827 1632 Size: 2228 66 1878 1371 1384 268 1917 Fragments arranged by size: 2228 1917 1878 1384 1371 268 66 AfeI AGC'GCT Cuts at: 3890 5834 3890 Size: 1944 7168 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8915 AflIII A'CryG_T Cuts at: 1203 4591 8011 8988 1203 Size: 3388 3420 977 1327 Fragments arranged by size: 3420 3388 1327 977 AgeI A'CCGG_T Cuts at: 7943 7943 Size: 9112 AhdI GACnn_n'nnGTC Cuts at: 7841 7841 Size: 9112 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9112 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4629 5741 1184 Size: 3445 1112 4555 Fragments arranged by size: 4555 3445 1112 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6324 6356 6324 Size: 32 9080 AlwNI CAG_nnn'CTG Cuts at: 2312 3651 6855 8427 2312 Size: 1339 3204 1572 2997 Fragments arranged by size: 3204 2997 1572 1339 ApaLI G'TGCA_C Cuts at: 7169 8325 7169 Size: 1156 7956 ApoI r'AATT_y Cuts at: 1493 2196 2958 3618 4145 4441 4570 4656 6421 Size: 703 762 660 527 296 129 86 1765 Cuts at: 6421 6647 6799 8908 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1765 1697 762 703 660 527 296 226 152 129 86 AseI AT'TA_AT Cuts at: 160 1032 5295 7666 160 Size: 872 4263 2371 1606 Fragments arranged by size: 4263 2371 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9112 AvaI C'yCGr_G Cuts at: 1870 1943 3513 6368 6437 1870 Size: 73 1570 2855 69 4545 Fragments arranged by size: 4545 2855 1570 73 69 AvrII C'CTAG_G Cuts at: 6265 6265 Size: 9112 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5631 5664 5631 Size: 33 9079 BamHI G'GATC_C Cuts at: 3625 6051 6442 6809 3625 Size: 2426 391 367 5928 Fragments arranged by size: 5928 2426 391 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 6433 7888 618 Size: 325 217 627 4646 1455 1842 Fragments arranged by size: 4646 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3356 4719 6431 1391 Size: 1965 1363 1712 4072 Fragments arranged by size: 4072 1965 1712 1363 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2593 4067 4143 5559 928 Size: 971 694 1474 76 1416 4481 Fragments arranged by size: 4481 1474 1416 971 694 76 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5409 5443 7303 7337 1903 Size: 34 3472 34 1860 34 3678 Fragments arranged by size: 3678 3472 1860 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2902 5632 5647 7004 8214 2902 Size: 2730 15 1357 1210 3800 Fragments arranged by size: 3800 2730 1357 1210 15 BclI T'GATC_A Cuts at: 1548 3985 4641 1548 Size: 2437 656 6019 Fragments arranged by size: 6019 2437 656 BfrBI ATG'CAT Cuts at: 3962 3962 Size: 9112 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7723 136 Size: 107 122 71 631 605 6051 1525 Fragments arranged by size: 6051 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3640 6109 9107 3640 Size: 2469 2998 3645 Fragments arranged by size: 3645 2998 2469 BlpI GC'TnA_GC Cuts at: 6529 6529 Size: 9112 Bme1580I G_kGCm'C Cuts at: 1790 2074 7173 8329 1790 Size: 284 5099 1156 2573 Fragments arranged by size: 5099 2573 1156 284 BmgBI CAC'GTC Cuts at: 1318 3154 6283 1318 Size: 1836 3129 4147 Fragments arranged by size: 4147 3129 1836 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2284 6843 7801 Size: 416 53 83 399 24 867 4559 958 Cuts at: 7801 442 Size: 1753 Fragments arranged by size: 4559 1753 958 867 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9112 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6152 6152 Size: 9112 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3402 4703 4904 5601 5821 6098 6256 7772 Size: 2163 1301 201 697 220 277 158 1516 Cuts at: 7772 1239 Size: 2579 Fragments arranged by size: 2579 2163 1516 1301 697 277 220 201 158 Bpu10I CC'TnA_GC Cuts at: 5714 5714 Size: 9112 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 7237 8102 8400 7237 Size: 865 298 7949 Fragments arranged by size: 7949 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3963 5753 7775 882 Size: 617 2464 1790 2022 2219 Fragments arranged by size: 2464 2219 2022 1790 617 BsaAI yAC'GTr Cuts at: 493 1204 4647 4940 6903 493 Size: 711 3443 293 1963 2702 Fragments arranged by size: 3443 2702 1963 711 293 BsaBI GATnn'nnATC Cuts at: 4688 4793 9106 4688 Size: 105 4313 4694 Fragments arranged by size: 4694 4313 105 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2151 6919 7301 7936 Size: 53 83 186 936 618 4768 382 635 Cuts at: 7936 275 Size: 1451 Fragments arranged by size: 4768 1451 936 635 618 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3396 7545 7943 8217 8364 8775 3396 Size: 4149 398 274 147 411 3733 Fragments arranged by size: 4149 3733 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3391 3400 3421 3430 6254 Size: 30 165 30 1988 9 21 9 2824 Cuts at: 6254 6284 1178 Size: 30 4006 Fragments arranged by size: 4006 2824 1988 165 30 30 30 21 9 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 5957 1186 Size: 4771 4341 BseYI C'CCAG_C Cuts at: 3478 3758 8315 8811 3478 Size: 280 4557 496 3779 Fragments arranged by size: 4557 3779 496 280 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2316 2381 4913 4962 1163 Size: 573 580 65 2532 49 5313 Fragments arranged by size: 5313 2532 580 573 65 49 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6562 7323 8351 1540 Size: 275 186 4561 761 1028 2301 Fragments arranged by size: 4561 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4121 6102 7173 7258 8329 4121 Size: 1981 1071 85 1071 4904 Fragments arranged by size: 4904 1981 1071 1071 85 BsmI GAATG_Cn' Cuts at: 3760 3964 4285 6623 6716 3760 Size: 204 321 2338 93 6156 Fragments arranged by size: 6156 2338 321 204 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2074 3356 4121 4719 6102 6431 7173 Size: 399 284 1282 765 598 1383 329 742 Cuts at: 7173 7258 8329 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1383 1282 1071 765 742 598 399 329 284 85 BspEI T'CCGG_A Cuts at: 8775 8775 Size: 9112 BspHI T'CATG_A Cuts at: 2031 2157 5968 7001 8731 2031 Size: 126 3811 1033 1730 2412 Fragments arranged by size: 3811 2412 1730 1033 126 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2297 6467 844 Size: 1453 4170 3489 Fragments arranged by size: 4170 3489 1453 BsrBI CCG'CTC Cuts at: 1394 1823 6999 1394 Size: 429 5176 3507 Fragments arranged by size: 5176 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2577 3093 3714 3921 5425 5694 7607 Size: 1183 1329 516 621 207 1504 269 1913 Cuts at: 7607 7781 65 Size: 174 1396 Fragments arranged by size: 1913 1504 1396 1329 1183 621 516 269 207 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6214 6388 7756 7943 1700 Size: 110 4404 174 1368 187 2869 Fragments arranged by size: 4404 2869 1368 187 174 110 BsrGI T'GTAC_A Cuts at: 96 3814 96 Size: 3718 5394 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9112 BssSI C'ACGA_G Cuts at: 5939 7172 8184 5939 Size: 1233 1012 6867 Fragments arranged by size: 6867 1233 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9112 BstBI TT'CG_AA Cuts at: 5551 7916 5551 Size: 2365 6747 BstEII G'GTnAC_C Cuts at: 1184 5672 1184 Size: 4488 4624 BstXI CCAn_nnnn'nTGG Cuts at: 2468 5914 2468 Size: 3446 5666 BstYI r'GATC_y Cuts at: 1972 3625 3640 6051 6109 6442 6809 7194 7211 Size: 1653 15 2411 58 333 367 385 17 Cuts at: 7211 8663 8749 8761 9107 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2411 1977 1653 1452 385 367 346 333 86 58 17 15 12 BstZ17I GTA'TAC Cuts at: 2431 6007 2431 Size: 3576 5536 BtgI C'CryG_G Cuts at: 513 1065 3786 4165 4272 4711 5677 5740 513 Size: 552 2721 379 107 439 966 63 3885 Fragments arranged by size: 3885 2721 966 552 439 379 107 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 1998 487 Size: 683 56 772 7601 Fragments arranged by size: 7601 772 683 56 BtsI GCAGTG_nn' Cuts at: 2304 5893 6331 6628 7422 7442 2304 Size: 3589 438 297 794 20 3974 Fragments arranged by size: 3974 3589 794 438 297 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9112 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2070 2105 3936 3971 529 Size: 35 1506 35 1831 35 5670 Fragments arranged by size: 5670 1831 1506 35 35 35 DraI TTT'AAA Cuts at: 6416 6416 Size: 9112 DraIII CAC_nnn'GTG Cuts at: 3157 3157 Size: 9112 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8119 809 Size: 507 6803 1802 Fragments arranged by size: 6803 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2009 3999 4210 Size: 54 1425 50 275 18 179 1990 211 Cuts at: 4210 6168 6216 6559 7448 9077 8 Size: 1958 48 343 889 1629 43 Fragments arranged by size: 1990 1958 1629 1425 889 343 275 211 179 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6559 1537 Size: 275 4747 4090 Fragments arranged by size: 4747 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3483 4787 5253 5330 7042 1353 Size: 2130 1304 466 77 1712 3423 Fragments arranged by size: 3423 2130 1712 1304 466 77 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3225 6064 7699 8073 8219 387 Size: 1064 138 1636 2839 1635 374 146 1280 Fragments arranged by size: 2839 1636 1635 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3402 3860 3926 4703 4904 5601 5804 Size: 393 1770 458 66 777 201 697 203 Cuts at: 5804 5821 6098 6256 7175 7772 8559 8827 1239 Size: 17 277 158 919 597 787 268 1524 Fragments arranged by size: 1770 1524 919 787 777 697 597 458 393 277 268 203 201 158 66 17 EcoNI CCTnn'n_nnAGG Cuts at: 5172 5172 Size: 9112 EcoO109I rG'GnC_Cy Cuts at: 2309 3296 4259 6360 2309 Size: 987 963 2101 5061 Fragments arranged by size: 5061 2101 987 963 EcoRI G'AATT_C Cuts at: 2958 6421 6799 2958 Size: 3463 378 5271 Fragments arranged by size: 5271 3463 378 EcoRV GAT'ATC Cuts at: 3283 3283 Size: 9112 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2420 2590 2949 2420 Size: 170 359 8583 Fragments arranged by size: 8583 359 170 FspI TGC'GCA Cuts at: 7618 7618 Size: 9112 HaeII r_GCGC'y Cuts at: 1678 3892 5836 8259 1678 Size: 2214 1944 2423 2531 Fragments arranged by size: 2531 2423 2214 1944 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2251 2283 2450 2482 2546 Size: 32 135 32 679 32 167 32 64 Cuts at: 2546 2578 2715 2747 2912 2944 3179 3211 3730 Size: 32 137 32 165 32 235 32 519 Cuts at: 3730 3762 3891 3923 5202 5234 5912 5944 6101 Size: 32 129 32 1279 32 678 32 157 Cuts at: 6101 6133 7753 7785 7827 7859 1373 Size: 32 1620 32 42 32 2626 Fragments arranged by size: 2626 1620 1279 679 678 519 235 167 165 157 137 135 129 64 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3931 4503 6456 6702 669 Size: 988 281 1993 572 1953 246 3079 Fragments arranged by size: 3079 1993 1953 988 572 281 246 HindIII A'AGCT_T Cuts at: 748 3872 3951 4305 748 Size: 3124 79 354 5555 Fragments arranged by size: 5555 3124 354 79 HpaI GTT'AAC Cuts at: 3931 6702 3931 Size: 2771 6341 KpnI G_GTAC'C Cuts at: 6437 6437 Size: 9112 MfeI C'AATT_G Cuts at: 2870 6711 2870 Size: 3841 5271 MluI A'CGCG_T Cuts at: 8988 8988 Size: 9112 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2481 4633 5071 5823 8226 8410 1305 Size: 1176 2152 438 752 2403 184 2007 Fragments arranged by size: 2403 2152 2007 1176 752 438 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2011 10 Size: 54 1425 343 179 7111 Fragments arranged by size: 7111 1425 343 179 54 MslI CAynn'nnrTG Cuts at: 518 1166 3753 4611 4874 4910 7070 7429 7588 Size: 648 2587 858 263 36 2160 359 159 Cuts at: 7588 518 Size: 2042 Fragments arranged by size: 2587 2160 2042 858 648 359 263 159 36 MspA1I CmG'CkG Cuts at: 1606 3101 3422 3707 3796 3991 4183 4713 5000 Size: 1495 321 285 89 195 192 530 287 Cuts at: 5000 5703 6292 6528 7205 8353 8598 1606 Size: 703 589 236 677 1148 245 2120 Fragments arranged by size: 2120 1495 1148 703 677 589 530 321 287 285 245 236 195 192 89 NcoI C'CATG_G Cuts at: 513 1065 3786 4165 5677 513 Size: 552 2721 379 1512 3948 Fragments arranged by size: 3948 2721 1512 552 379 NdeI CA'TA_TG Cuts at: 387 2917 387 Size: 2530 6582 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9112 NotI GC'GGCC_GC Cuts at: 6559 6559 Size: 9112 NsiI A_TGCA'T Cuts at: 3964 3964 Size: 9112 NspI r_CATG'y Cuts at: 1132 1229 4551 4595 6151 6259 6470 8015 1132 Size: 97 3322 44 1556 108 211 1545 2229 Fragments arranged by size: 3322 2229 1556 1545 211 108 97 44 PasI CC'CwG_GG Cuts at: 1348 3117 1348 Size: 1769 7343 PciI A'CATG_T Cuts at: 4591 8011 4591 Size: 3420 5692 PflMI CCAn_nnn'nTGG Cuts at: 1108 3916 5669 1108 Size: 2808 1753 4551 Fragments arranged by size: 4551 2808 1753 PfoI T'CCnGG_A Cuts at: 2140 2335 2410 4881 5491 5913 2140 Size: 195 75 2471 610 422 5339 Fragments arranged by size: 5339 2471 610 422 195 75 PmeI GTTT'AAAC Cuts at: 6416 6416 Size: 9112 PmlI CAC'GTG Cuts at: 4647 4647 Size: 9112 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7181 7213 8694 8726 7181 Size: 32 1481 32 7567 Fragments arranged by size: 7567 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2309 3296 4259 6360 2309 Size: 987 963 2101 5061 Fragments arranged by size: 5061 2101 987 963 PshAI GACnn'nnGTC Cuts at: 1613 2331 1613 Size: 718 8394 PsiI TTA'TAA Cuts at: 6682 6682 Size: 9112 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2893 2925 5437 5469 2893 Size: 32 2512 32 6536 Fragments arranged by size: 6536 2512 32 32 PstI C_TGCA'G Cuts at: 830 2496 3574 5005 6464 830 Size: 1666 1078 1431 1459 3478 Fragments arranged by size: 3478 1666 1459 1431 1078 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9112 PvuII CAG'CTG Cuts at: 3101 3422 3707 3796 3991 4183 5000 6292 3101 Size: 321 285 89 195 192 817 1292 5921 Fragments arranged by size: 5921 1292 817 321 285 195 192 89 SacII CC_GC'GG Cuts at: 4714 4714 Size: 9112 SalI G'TCGA_C Cuts at: 1936 6454 1936 Size: 4518 4594 SanDI GG'GwC_CC Cuts at: 6360 6360 Size: 9112 SapI GCTCTTCn'nnn_ Cuts at: 4787 5253 4787 Size: 466 8646 SbfI CC_TGCA'GG Cuts at: 3574 6464 3574 Size: 2890 6222 ScaI AGT'ACT Cuts at: 3403 4581 7360 9072 3403 Size: 1178 2779 1712 3443 Fragments arranged by size: 3443 2779 1712 1178 SfcI C'TryA_G Cuts at: 826 1045 2124 2492 2533 3570 3579 5001 5011 Size: 219 1079 368 41 1037 9 1422 10 Cuts at: 5011 5345 5381 5431 6460 7595 8276 8467 826 Size: 334 36 50 1029 1135 681 191 1471 Fragments arranged by size: 1471 1422 1135 1079 1037 1029 681 368 334 219 191 50 41 36 10 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9112 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9112 SmaI CCC'GGG Cuts at: 1872 6439 1872 Size: 4567 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 7216 8117 8379 820 Size: 197 926 5273 901 262 1553 Fragments arranged by size: 5273 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6903 493 Size: 6410 2702 SpeI A'CTAG_T Cuts at: 152 3404 6404 152 Size: 3252 3000 2860 Fragments arranged by size: 3252 3000 2860 SphI G_CATG'C Cuts at: 1132 6151 6259 6470 1132 Size: 5019 108 211 3774 Fragments arranged by size: 5019 3774 211 108 SspI AAT'ATT Cuts at: 5 52 3164 4093 7036 5 Size: 47 3112 929 2943 2081 Fragments arranged by size: 3112 2943 2081 929 47 StuI AGG'CCT Cuts at: 1517 3804 1517 Size: 2287 6825 StyI C'CwwG_G Cuts at: 513 1065 2565 3631 3662 3786 4165 5119 5677 Size: 552 1500 1066 31 124 379 954 558 Cuts at: 5677 5881 6265 6551 513 Size: 204 384 286 3074 Fragments arranged by size: 3074 1500 1066 954 558 552 384 379 286 204 124 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6212 6218 7139 7156 7309 7494 7939 Size: 1255 4632 6 921 17 153 185 445 Cuts at: 7939 325 Size: 1498 Fragments arranged by size: 4632 1498 1255 921 445 185 153 17 6 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3090 3401 3814 Size: 274 80 33 51 1443 1113 311 413 Cuts at: 3814 4579 5444 7358 9070 96 Size: 765 865 1914 1712 138 Fragments arranged by size: 1914 1712 1443 1113 865 765 413 311 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2625 2657 2704 2736 3439 3471 9072 9104 2625 Size: 32 47 32 703 32 5601 32 2633 Fragments arranged by size: 5601 2633 703 47 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4070 1316 Size: 2754 6358 XbaI T'CTAG_A Cuts at: 6448 6448 Size: 9112 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3793 4291 3793 Size: 498 8614 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9112 XmaI C'CCGG_G Cuts at: 1870 6437 1870 Size: 4567 4545 XmnI GAAnn'nnTTC Cuts at: 5964 7241 5964 Size: 1277 7835 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2152 6920 276 Size: 53 83 186 936 618 4768 2468 Fragments arranged by size: 4768 2468 936 618 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI BbeI BbvCI BsiWI BsmBI Bsu36I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI RsrII SacI SexAI SfiI SfoI SrfI SwaI