(Linear) (Six-base) MAPSORT of: KIEE9444.seq Check: 1137 from: 1 to: 4407 KIEE9444.orf With 182 enzymes: SgfI * July 3, 2012 19:02 .. AatII G_ACGT'C Cuts at: 0 149 4407 Size: 149 4258 AccI GT'mk_AC Cuts at: 0 425 3002 4001 4407 Size: 425 2577 999 406 Fragments arranged by size: 2577 999 425 406 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1855 1921 3799 4407 Size: 1855 66 1878 608 Fragments arranged by size: 1878 1855 608 66 AfeI AGC'GCT Cuts at: 0 1885 3829 4407 Size: 1885 1944 578 Fragments arranged by size: 1944 1885 578 AflIII A'CryG_T Cuts at: 0 2586 4407 Size: 2586 1821 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2624 3736 4407 Size: 2624 1112 671 Fragments arranged by size: 2624 1112 671 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 4319 4351 4407 Size: 4319 32 56 Fragments arranged by size: 4319 56 32 AlwNI CAG_nnn'CTG Cuts at: 0 307 1646 4407 Size: 307 1339 2761 Fragments arranged by size: 2761 1339 307 ApoI r'AATT_y Cuts at: 0 191 953 1613 2140 2436 2565 2651 4407 Size: 191 762 660 527 296 129 86 1756 Fragments arranged by size: 1756 762 660 527 296 191 129 86 AseI AT'TA_AT Cuts at: 0 3290 4407 Size: 3290 1117 AvaI C'yCGr_G Cuts at: 0 1508 4363 4407 Size: 1508 2855 44 Fragments arranged by size: 2855 1508 44 AvrII C'CTAG_G Cuts at: 0 4260 4407 Size: 4260 147 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 3626 3659 4407 Size: 3626 33 748 Fragments arranged by size: 3626 748 33 BamHI G'GATC_C Cuts at: 0 1620 4046 4407 Size: 1620 2426 361 Fragments arranged by size: 2426 1620 361 BanII G_rGCy'C Cuts at: 0 1351 2714 4407 Size: 1351 1363 1693 Fragments arranged by size: 1693 1363 1351 BbsI GAAGACnn'nnnn_ Cuts at: 0 588 2062 2138 3554 4407 Size: 588 1474 76 1416 853 Fragments arranged by size: 1474 1416 853 588 76 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3404 3438 4407 Size: 3404 34 969 Fragments arranged by size: 3404 969 34 BciVI GTATCCnnnnn_n' Cuts at: 0 897 3627 3642 4407 Size: 897 2730 15 765 Fragments arranged by size: 2730 897 765 15 BclI T'GATC_A Cuts at: 0 1980 2636 4407 Size: 1980 656 1771 Fragments arranged by size: 1980 1771 656 BfrBI ATG'CAT Cuts at: 0 1957 4407 Size: 1957 2450 BglII A'GATC_T Cuts at: 0 1635 4104 4407 Size: 1635 2469 303 Fragments arranged by size: 2469 1635 303 Bme1580I G_kGCm'C Cuts at: 0 69 4407 Size: 69 4338 BmgBI CAC'GTC Cuts at: 0 1149 4278 4407 Size: 1149 3129 129 Fragments arranged by size: 3129 1149 129 BmrI ACTGGGnnnn_n' Cuts at: 0 279 4407 Size: 279 4128 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 4147 4407 Size: 4147 260 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1397 2698 2899 3596 3816 4093 4251 4407 Size: 1397 1301 201 697 220 277 158 156 Fragments arranged by size: 1397 1301 697 277 220 201 158 156 Bpu10I CC'TnA_GC Cuts at: 0 3709 4407 Size: 3709 698 BsaI GGTCTCn'nnnn_ Cuts at: 0 1958 3748 4407 Size: 1958 1790 659 Fragments arranged by size: 1958 1790 659 BsaAI yAC'GTr Cuts at: 0 2642 2935 4407 Size: 2642 293 1472 Fragments arranged by size: 2642 1472 293 BsaBI GATnn'nnATC Cuts at: 0 2683 2788 4407 Size: 2683 105 1619 Fragments arranged by size: 2683 1619 105 BsaHI Gr'CG_yC Cuts at: 0 146 4407 Size: 146 4261 BsaWI w'CCGG_w Cuts at: 0 1391 4407 Size: 1391 3016 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1386 1395 1416 1425 4249 4279 4407 Size: 1386 9 21 9 2824 30 128 Fragments arranged by size: 2824 1386 128 30 21 9 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 3952 4407 Size: 3952 455 BseYI C'CCAG_C Cuts at: 0 1473 1753 4407 Size: 1473 280 2654 Fragments arranged by size: 2654 1473 280 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 311 376 2908 2957 4407 Size: 311 65 2532 49 1450 Fragments arranged by size: 2532 1450 311 65 49 BsiHKAI G_wGCw'C Cuts at: 0 2116 4097 4407 Size: 2116 1981 310 Fragments arranged by size: 2116 1981 310 BsmI GAATG_Cn' Cuts at: 0 1755 1959 2280 4407 Size: 1755 204 321 2127 Fragments arranged by size: 2127 1755 321 204 Bsp1286I G_dGCh'C Cuts at: 0 69 1351 2116 2714 4097 4407 Size: 69 1282 765 598 1383 310 Fragments arranged by size: 1383 1282 765 598 310 69 BspHI T'CATG_A Cuts at: 0 26 152 3963 4407 Size: 26 126 3811 444 Fragments arranged by size: 3811 444 126 26 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 292 4407 Size: 292 4115 BsrDI GCAATG_nn' Cuts at: 0 572 1088 1709 1916 3420 3689 4407 Size: 572 516 621 207 1504 269 718 Fragments arranged by size: 1504 718 621 572 516 269 207 BsrFI r'CCGG_y Cuts at: 0 4209 4383 4407 Size: 4209 174 24 Fragments arranged by size: 4209 174 24 BsrGI T'GTAC_A Cuts at: 0 1809 4407 Size: 1809 2598 BssSI C'ACGA_G Cuts at: 0 3934 4407 Size: 3934 473 BstBI TT'CG_AA Cuts at: 0 3546 4407 Size: 3546 861 BstEII G'GTnAC_C Cuts at: 0 3667 4407 Size: 3667 740 BstXI CCAn_nnnn'nTGG Cuts at: 0 463 3909 4407 Size: 463 3446 498 Fragments arranged by size: 3446 498 463 BstYI r'GATC_y Cuts at: 0 1620 1635 4046 4104 4407 Size: 1620 15 2411 58 303 Fragments arranged by size: 2411 1620 303 58 15 BstZ17I GTA'TAC Cuts at: 0 426 4002 4407 Size: 426 3576 405 Fragments arranged by size: 3576 426 405 BtgI C'CryG_G Cuts at: 0 1781 2160 2267 2706 3672 3735 4407 Size: 1781 379 107 439 966 63 672 Fragments arranged by size: 1781 966 672 439 379 107 63 BtsI GCAGTG_nn' Cuts at: 0 299 3888 4326 4407 Size: 299 3589 438 81 Fragments arranged by size: 3589 438 299 81 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 65 100 1931 1966 4407 Size: 65 35 1831 35 2441 Fragments arranged by size: 2441 1831 65 35 35 DraIII CAC_nnn'GTG Cuts at: 0 1152 4407 Size: 1152 3255 EaeI y'GGCC_r Cuts at: 0 4 1994 2205 4163 4211 4407 Size: 4 1990 211 1958 48 196 Fragments arranged by size: 1990 1958 211 196 48 4 EarI CTCTTCn'nnn_ Cuts at: 0 1478 2782 3248 3325 4407 Size: 1478 1304 466 77 1082 Fragments arranged by size: 1478 1304 1082 466 77 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1220 4059 4407 Size: 1220 2839 348 Fragments arranged by size: 2839 1220 348 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1397 1855 1921 2698 2899 3596 3799 3816 Size: 1397 458 66 777 201 697 203 17 Cuts at: 3816 4093 4251 4407 Size: 277 158 156 Fragments arranged by size: 1397 777 697 458 277 203 201 158 156 66 17 EcoNI CCTnn'n_nnAGG Cuts at: 0 3167 4407 Size: 3167 1240 EcoO109I rG'GnC_Cy Cuts at: 0 304 1291 2254 4355 4407 Size: 304 987 963 2101 52 Fragments arranged by size: 2101 987 963 304 52 EcoRI G'AATT_C Cuts at: 0 953 4407 Size: 953 3454 EcoRV GAT'ATC Cuts at: 0 1278 4407 Size: 1278 3129 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 415 585 944 4407 Size: 415 170 359 3463 Fragments arranged by size: 3463 415 359 170 HaeII r_GCGC'y Cuts at: 0 1887 3831 4407 Size: 1887 1944 576 Fragments arranged by size: 1944 1887 576 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 246 278 445 477 541 573 710 742 Size: 246 32 167 32 64 32 137 32 Cuts at: 742 907 939 1174 1206 1725 1757 1886 1918 Size: 165 32 235 32 519 32 129 32 Cuts at: 1918 3197 3229 3907 3939 4096 4128 4407 Size: 1279 32 678 32 157 32 279 Fragments arranged by size: 1279 678 519 279 246 235 167 165 157 137 129 64 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1926 2498 4407 Size: 1926 572 1909 Fragments arranged by size: 1926 1909 572 HindIII A'AGCT_T Cuts at: 0 1867 1946 2300 4407 Size: 1867 79 354 2107 Fragments arranged by size: 2107 1867 354 79 HpaI GTT'AAC Cuts at: 0 1926 4407 Size: 1926 2481 MfeI C'AATT_G Cuts at: 0 865 4407 Size: 865 3542 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 476 2628 3066 3818 4407 Size: 476 2152 438 752 589 Fragments arranged by size: 2152 752 589 476 438 MscI TGG'CCA Cuts at: 0 6 4407 Size: 6 4401 MslI CAynn'nnrTG Cuts at: 0 1748 2606 2869 2905 4407 Size: 1748 858 263 36 1502 Fragments arranged by size: 1748 1502 858 263 36 MspA1I CmG'CkG Cuts at: 0 1096 1417 1702 1791 1986 2178 2708 2995 Size: 1096 321 285 89 195 192 530 287 Cuts at: 2995 3698 4287 4407 Size: 703 589 120 Fragments arranged by size: 1096 703 589 530 321 287 285 195 192 120 89 NcoI C'CATG_G Cuts at: 0 1781 2160 3672 4407 Size: 1781 379 1512 735 Fragments arranged by size: 1781 1512 735 379 NdeI CA'TA_TG Cuts at: 0 912 4407 Size: 912 3495 NsiI A_TGCA'T Cuts at: 0 1959 4407 Size: 1959 2448 NspI r_CATG'y Cuts at: 0 2546 2590 4146 4254 4407 Size: 2546 44 1556 108 153 Fragments arranged by size: 2546 1556 153 108 44 PasI CC'CwG_GG Cuts at: 0 1112 4407 Size: 1112 3295 PciI A'CATG_T Cuts at: 0 2586 4407 Size: 2586 1821 PflMI CCAn_nnn'nTGG Cuts at: 0 1911 3664 4407 Size: 1911 1753 743 Fragments arranged by size: 1911 1753 743 PfoI T'CCnGG_A Cuts at: 0 135 330 405 2876 3486 3908 4407 Size: 135 195 75 2471 610 422 499 Fragments arranged by size: 2471 610 499 422 195 135 75 PmlI CAC'GTG Cuts at: 0 2642 4407 Size: 2642 1765 PpuMI rG'GwC_Cy Cuts at: 0 304 1291 2254 4355 4407 Size: 304 987 963 2101 52 Fragments arranged by size: 2101 987 963 304 52 PshAI GACnn'nnGTC Cuts at: 0 326 4407 Size: 326 4081 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 888 920 3432 3464 4407 Size: 888 32 2512 32 943 Fragments arranged by size: 2512 943 888 32 32 PstI C_TGCA'G Cuts at: 0 491 1569 3000 4407 Size: 491 1078 1431 1407 Fragments arranged by size: 1431 1407 1078 491 PvuII CAG'CTG Cuts at: 0 1096 1417 1702 1791 1986 2178 2995 4287 Size: 1096 321 285 89 195 192 817 1292 Cuts at: 4287 4407 Size: 120 Fragments arranged by size: 1292 1096 817 321 285 195 192 120 89 SacII CC_GC'GG Cuts at: 0 2709 4407 Size: 2709 1698 SanDI GG'GwC_CC Cuts at: 0 4355 4407 Size: 4355 52 SapI GCTCTTCn'nnn_ Cuts at: 0 2782 3248 4407 Size: 2782 466 1159 Fragments arranged by size: 2782 1159 466 SbfI CC_TGCA'GG Cuts at: 0 1569 4407 Size: 1569 2838 ScaI AGT'ACT Cuts at: 0 1398 2576 4407 Size: 1398 1178 1831 Fragments arranged by size: 1831 1398 1178 SfcI C'TryA_G Cuts at: 0 119 487 528 1565 1574 2996 3006 3340 Size: 119 368 41 1037 9 1422 10 334 Cuts at: 3340 3376 3426 4407 Size: 36 50 981 Fragments arranged by size: 1422 1037 981 368 334 119 50 41 36 10 9 SpeI A'CTAG_T Cuts at: 0 1399 4399 4407 Size: 1399 3000 8 Fragments arranged by size: 3000 1399 8 SphI G_CATG'C Cuts at: 0 4146 4254 4407 Size: 4146 108 153 Fragments arranged by size: 4146 153 108 SspI AAT'ATT Cuts at: 0 1159 2088 4407 Size: 1159 929 2319 Fragments arranged by size: 2319 1159 929 StuI AGG'CCT Cuts at: 0 1799 4407 Size: 1799 2608 StyI C'CwwG_G Cuts at: 0 560 1626 1657 1781 2160 3114 3672 3876 Size: 560 1066 31 124 379 954 558 204 Cuts at: 3876 4260 4407 Size: 384 147 Fragments arranged by size: 1066 954 560 558 384 379 204 147 124 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 4207 4213 4407 Size: 4207 6 194 Fragments arranged by size: 4207 194 6 TatI w'GTAC_w Cuts at: 0 1085 1396 1809 2574 3439 4407 Size: 1085 311 413 765 865 968 Fragments arranged by size: 1085 968 865 765 413 311 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 620 652 699 731 1434 1466 4407 Size: 620 32 47 32 703 32 2941 Fragments arranged by size: 2941 703 620 47 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2065 4407 Size: 2065 2342 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1788 2286 4407 Size: 1788 498 2121 Fragments arranged by size: 2121 1788 498 XmnI GAAnn'nnTTC Cuts at: 0 3959 4407 Size: 3959 448 ZraI GAC'GTC Cuts at: 0 147 4407 Size: 147 4260 Enzymes that do cut: AatII AccI AcuI AfeI AflIII AlfI AloI AlwNI ApoI AseI AvaI AvrII BaeI BamHI BanII BbsI BcgI BciVI BclI BfrBI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI BstBI BstEII BstXI BstYI BstZ17I BtgI BtsI CspCI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI PshAI PsrI PstI PvuII SacII SanDI SapI SbfI ScaI SfcI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XcmI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AflII AgeI AhdI AleI ApaI ApaLI AscI AsiSI BanI BbeI BbvCI BglI BlpI BmtI BpuEI BsiEI BsiWI BsmBI BspEI BsrBI BssHII BstAPI Bsu36I BtgZI ClaI DraI DrdI EagI EcoICRI FseI FspI FspAI KasI KpnI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PmeI PpiI PsiI PspOMI PspXI PvuI RsrII SacI SalI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SrfI SwaI XbaI XhoI XmaI