(Linear) (Six-base) MAPSORT of: KIEE3310.seq Check: 2807 from: 1 to: 2457 KIEE3310.orf With 182 enzymes: SgfI * July 3, 2012 17:37 .. AatII G_ACGT'C Cuts at: 0 1676 2457 Size: 1676 781 AccI GT'mk_AC Cuts at: 0 2358 2457 Size: 2358 99 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1933 1975 2457 Size: 1933 42 482 Fragments arranged by size: 1933 482 42 AflII C'TTAA_G Cuts at: 0 1792 2457 Size: 1792 665 AhdI GACnn_n'nnGTC Cuts at: 0 1389 1575 2457 Size: 1389 186 882 Fragments arranged by size: 1389 882 186 AlwNI CAG_nnn'CTG Cuts at: 0 124 205 949 1537 2457 Size: 124 81 744 588 920 Fragments arranged by size: 920 744 588 124 81 ApoI r'AATT_y Cuts at: 0 1459 2457 Size: 1459 998 AseI AT'TA_AT Cuts at: 0 2041 2457 Size: 2041 416 AvaI C'yCGr_G Cuts at: 0 890 2457 Size: 890 1567 BamHI G'GATC_C Cuts at: 0 837 2457 Size: 837 1620 BanI G'GyrC_C Cuts at: 0 575 692 770 1648 2457 Size: 575 117 78 878 809 Fragments arranged by size: 878 809 575 117 78 BanII G_rGCy'C Cuts at: 0 221 491 2457 Size: 221 270 1966 Fragments arranged by size: 1966 270 221 BbsI GAAGACnn'nnnn_ Cuts at: 0 1306 1724 2457 Size: 1306 418 733 Fragments arranged by size: 1306 733 418 BbvCI CC'TCA_GC Cuts at: 0 1712 2457 Size: 1712 745 BciVI GTATCCnnnnn_n' Cuts at: 0 1014 2457 Size: 1014 1443 BglII A'GATC_T Cuts at: 0 1482 2121 2407 2457 Size: 1482 639 286 50 Fragments arranged by size: 1482 639 286 50 Bme1580I G_kGCm'C Cuts at: 0 2250 2457 Size: 2250 207 BmrI ACTGGGnnnn_n' Cuts at: 0 46 2457 Size: 46 2411 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 126 2457 Size: 126 2331 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 475 2249 2457 Size: 475 1774 208 Fragments arranged by size: 1774 475 208 Bpu10I CC'TnA_GC Cuts at: 0 1712 2457 Size: 1712 745 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1876 2457 Size: 1876 581 BsaI GGTCTCn'nnnn_ Cuts at: 0 364 1942 2177 2457 Size: 364 1578 235 280 Fragments arranged by size: 1578 364 280 235 BsaBI GATnn'nnATC Cuts at: 0 338 2457 Size: 338 2119 BsaHI Gr'CG_yC Cuts at: 0 1673 2457 Size: 1673 784 BsaWI w'CCGG_w Cuts at: 0 112 2457 Size: 112 2345 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1371 1401 1999 2029 2457 Size: 1371 30 598 30 428 Fragments arranged by size: 1371 598 428 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 268 499 616 655 733 889 1111 1168 Size: 268 231 117 39 78 156 222 57 Cuts at: 1168 1171 1918 2224 2457 Size: 3 747 306 233 Fragments arranged by size: 747 306 268 233 231 222 156 117 78 57 39 3 BseYI C'CCAG_C Cuts at: 0 1330 2457 Size: 1330 1127 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 320 1871 2396 2457 Size: 320 1551 525 61 Fragments arranged by size: 1551 525 320 61 BsiHKAI G_wGCw'C Cuts at: 0 491 2457 Size: 491 1966 Bsp1286I G_dGCh'C Cuts at: 0 221 491 2250 2457 Size: 221 270 1759 207 Fragments arranged by size: 1759 270 221 207 BspEI T'CCGG_A Cuts at: 0 112 2457 Size: 112 2345 BsrDI GCAATG_nn' Cuts at: 0 1404 2457 Size: 1404 1053 BstAPI GCAn_nnn'nTGC Cuts at: 0 1929 2457 Size: 1929 528 BstYI r'GATC_y Cuts at: 0 837 1368 1482 1895 1916 2121 2232 2407 Size: 837 531 114 413 21 205 111 175 Cuts at: 2407 2457 Size: 50 Fragments arranged by size: 837 531 413 205 175 114 111 50 21 Bsu36I CC'TnA_GG Cuts at: 0 656 2457 Size: 656 1801 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1655 2457 Size: 1655 802 BtsI GCAGTG_nn' Cuts at: 0 1985 2426 2457 Size: 1985 441 31 Fragments arranged by size: 1985 441 31 DraIII CAC_nnn'GTG Cuts at: 0 2421 2457 Size: 2421 36 DrdI GACnn_nn'nnGTC Cuts at: 0 2287 2457 Size: 2287 170 EarI CTCTTCn'nnn_ Cuts at: 0 362 490 791 1165 1202 1222 1355 1442 Size: 362 128 301 374 37 20 133 87 Cuts at: 1442 2175 2457 Size: 733 282 Fragments arranged by size: 733 374 362 301 282 133 128 87 37 20 EcoICRI GAG'CTC Cuts at: 0 489 2457 Size: 489 1968 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 475 1933 1975 2249 2457 Size: 475 1458 42 274 208 Fragments arranged by size: 1458 475 274 208 42 EcoNI CCTnn'n_nnAGG Cuts at: 0 2191 2457 Size: 2191 266 EcoO109I rG'GnC_Cy Cuts at: 0 759 916 928 1803 2259 2457 Size: 759 157 12 875 456 198 Fragments arranged by size: 875 759 456 198 157 12 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1429 2457 Size: 1429 1028 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 61 93 265 271 297 303 943 975 Size: 61 32 172 6 26 6 640 32 Cuts at: 975 1201 1233 1258 1290 1375 1407 1561 1593 Size: 226 32 25 32 85 32 154 32 Cuts at: 1593 1999 2031 2457 Size: 406 32 426 Fragments arranged by size: 640 426 406 226 172 154 85 61 32 32 32 32 32 32 32 26 25 6 6 HincII GTy'rAC Cuts at: 0 2359 2457 Size: 2359 98 MslI CAynn'nnrTG Cuts at: 0 428 2457 Size: 428 2029 MspA1I CmG'CkG Cuts at: 0 20 2457 Size: 20 2437 NdeI CA'TA_TG Cuts at: 0 1061 1565 2457 Size: 1061 504 892 Fragments arranged by size: 1061 892 504 NspI r_CATG'y Cuts at: 0 427 2457 Size: 427 2030 PfoI T'CCnGG_A Cuts at: 0 1898 2235 2457 Size: 1898 337 222 Fragments arranged by size: 1898 337 222 PpuMI rG'GwC_Cy Cuts at: 0 759 1803 2259 2457 Size: 759 1044 456 198 Fragments arranged by size: 1044 759 456 198 SacI G_AGCT'C Cuts at: 0 491 2457 Size: 491 1966 SalI G'TCGA_C Cuts at: 0 2357 2457 Size: 2357 100 SanDI GG'GwC_CC Cuts at: 0 2259 2457 Size: 2259 198 SapI GCTCTTCn'nnn_ Cuts at: 0 791 2457 Size: 791 1666 SexAI A'CCwGG_T Cuts at: 0 384 2457 Size: 384 2073 SfcI C'TryA_G Cuts at: 0 1778 2457 Size: 1778 679 SmlI C'TyrA_G Cuts at: 0 1792 1891 2457 Size: 1792 99 566 Fragments arranged by size: 1792 566 99 SpeI A'CTAG_T Cuts at: 0 157 2457 Size: 157 2300 SphI G_CATG'C Cuts at: 0 427 2457 Size: 427 2030 SspI AAT'ATT Cuts at: 0 13 2457 Size: 13 2444 StuI AGG'CCT Cuts at: 0 2061 2457 Size: 2061 396 StyI C'CwwG_G Cuts at: 0 571 688 844 2003 2457 Size: 571 117 156 1159 454 Fragments arranged by size: 1159 571 454 156 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2190 2457 Size: 2190 267 TatI w'GTAC_w Cuts at: 0 54 2457 Size: 54 2403 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 179 211 1371 1403 1880 1912 1964 1996 Size: 179 32 1160 32 477 32 52 32 Cuts at: 1996 2457 Size: 461 Fragments arranged by size: 1160 477 461 179 52 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1672 2457 Size: 1672 785 XbaI T'CTAG_A Cuts at: 0 2124 2457 Size: 2124 333 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 34 2184 2304 2457 Size: 34 2150 120 153 Fragments arranged by size: 2150 153 120 34 XmnI GAAnn'nnTTC Cuts at: 0 800 2457 Size: 800 1657 ZraI GAC'GTC Cuts at: 0 1674 2457 Size: 1674 783 Enzymes that do cut: AatII AccI AcuI AflII AhdI AlwNI ApoI AseI AvaI BamHI BanI BanII BbsI BbvCI BciVI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BsrDI BstAPI BstYI Bsu36I BtgZI BtsI DraIII DrdI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII MslI MspA1I NdeI NspI PfoI PpuMI SacI SalI SanDI SapI SexAI SfcI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflIII AgeI AleI AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI BbeI BcgI BclI BfrBI BglI BlpI BmgBI BmtI BsaAI BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I BtgI ClaI CspCI DraI EaeI EagI EciI EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SwaI XhoI XmaI