(Linear) (Six-base) MAPSORT of: KIEE3258.seq Check: 9080 from: 1 to: 1851 KIEE3258.orf With 182 enzymes: SgfI * July 3, 2012 17:32 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 26 91 95 247 1619 1851 Size: 26 65 4 152 1372 232 Fragments arranged by size: 1372 232 152 65 26 4 AflII C'TTAA_G Cuts at: 0 481 1851 Size: 481 1370 AflIII A'CryG_T Cuts at: 0 1455 1851 Size: 1455 396 AleI CACnn'nnGTG Cuts at: 0 462 1851 Size: 462 1389 AlwNI CAG_nnn'CTG Cuts at: 0 1189 1851 Size: 1189 662 ApaI G_GGCC'C Cuts at: 0 1718 1851 Size: 1718 133 ApoI r'AATT_y Cuts at: 0 411 516 1068 1851 Size: 411 105 552 783 Fragments arranged by size: 783 552 411 105 AseI AT'TA_AT Cuts at: 0 1556 1851 Size: 1556 295 AvaI C'yCGr_G Cuts at: 0 298 1851 Size: 298 1553 BanII G_rGCy'C Cuts at: 0 1292 1718 1851 Size: 1292 426 133 Fragments arranged by size: 1292 426 133 BbsI GAAGACnn'nnnn_ Cuts at: 0 329 594 749 874 1094 1851 Size: 329 265 155 125 220 757 Fragments arranged by size: 757 329 265 220 155 125 BclI T'GATC_A Cuts at: 0 164 1851 Size: 164 1687 BglII A'GATC_T Cuts at: 0 1414 1761 1851 Size: 1414 347 90 Fragments arranged by size: 1414 347 90 Bme1580I G_kGCm'C Cuts at: 0 1718 1851 Size: 1718 133 BmrI ACTGGGnnnn_n' Cuts at: 0 146 748 1121 1761 1851 Size: 146 602 373 640 90 Fragments arranged by size: 640 602 373 146 90 Bpu10I CC'TnA_GC Cuts at: 0 852 1851 Size: 852 999 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1047 1851 Size: 1047 804 BsaBI GATnn'nnATC Cuts at: 0 233 1057 1419 1760 1851 Size: 233 824 362 341 91 Fragments arranged by size: 824 362 341 233 91 BsaWI w'CCGG_w Cuts at: 0 1140 1851 Size: 1140 711 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 563 676 775 1851 Size: 563 113 99 1076 Fragments arranged by size: 1076 563 113 99 BseYI C'CCAG_C Cuts at: 0 612 1264 1851 Size: 612 652 587 Fragments arranged by size: 652 612 587 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 365 1352 1851 Size: 365 987 499 Fragments arranged by size: 987 499 365 Bsp1286I G_dGCh'C Cuts at: 0 1292 1718 1851 Size: 1292 426 133 Fragments arranged by size: 1292 426 133 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1616 1851 Size: 1616 235 BsrBI CCG'CTC Cuts at: 0 1146 1851 Size: 1146 705 BsrDI GCAATG_nn' Cuts at: 0 713 1844 1851 Size: 713 1131 7 Fragments arranged by size: 1131 713 7 BstEII G'GTnAC_C Cuts at: 0 1603 1851 Size: 1603 248 BstXI CCAn_nnnn'nTGG Cuts at: 0 25 1851 Size: 25 1826 BstYI r'GATC_y Cuts at: 0 1414 1761 1851 Size: 1414 347 90 Fragments arranged by size: 1414 347 90 Bsu36I CC'TnA_GG Cuts at: 0 124 1851 Size: 124 1727 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 296 1851 Size: 296 1555 BtsI GCAGTG_nn' Cuts at: 0 469 1851 Size: 469 1382 DraI TTT'AAA Cuts at: 0 537 1310 1851 Size: 537 773 541 Fragments arranged by size: 773 541 537 DraIII CAC_nnn'GTG Cuts at: 0 316 1258 1851 Size: 316 942 593 Fragments arranged by size: 942 593 316 EaeI y'GGCC_r Cuts at: 0 466 1523 1851 Size: 466 1057 328 Fragments arranged by size: 1057 466 328 EarI CTCTTCn'nnn_ Cuts at: 0 506 782 1610 1851 Size: 506 276 828 241 Fragments arranged by size: 828 506 276 241 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 26 91 95 247 1619 1851 Size: 26 65 4 152 1372 232 Fragments arranged by size: 1372 232 152 65 26 4 EcoNI CCTnn'n_nnAGG Cuts at: 0 1586 1851 Size: 1586 265 EcoO109I rG'GnC_Cy Cuts at: 0 1275 1714 1715 1851 Size: 1275 439 1 136 Fragments arranged by size: 1275 439 136 1 EcoRI G'AATT_C Cuts at: 0 411 516 1851 Size: 411 105 1335 Fragments arranged by size: 1335 411 105 EcoRV GAT'ATC Cuts at: 0 696 1851 Size: 696 1155 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 898 1415 1851 Size: 898 517 436 Fragments arranged by size: 898 517 436 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 361 393 892 904 924 936 1021 1053 Size: 361 32 499 12 20 12 85 32 Cuts at: 1053 1165 1197 1851 Size: 112 32 654 Fragments arranged by size: 654 499 361 112 85 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 0 1167 1851 Size: 1167 684 HindIII A'AGCT_T Cuts at: 0 347 478 1851 Size: 347 131 1373 Fragments arranged by size: 1373 347 131 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 110 219 1851 Size: 110 109 1632 Fragments arranged by size: 1632 110 109 MscI TGG'CCA Cuts at: 0 468 1525 1851 Size: 468 1057 326 Fragments arranged by size: 1057 468 326 MslI CAynn'nnrTG Cuts at: 0 462 1851 Size: 462 1389 MspA1I CmG'CkG Cuts at: 0 102 1050 1851 Size: 102 948 801 Fragments arranged by size: 948 801 102 NdeI CA'TA_TG Cuts at: 0 1479 1851 Size: 1479 372 NspI r_CATG'y Cuts at: 0 492 1459 1851 Size: 492 967 392 Fragments arranged by size: 967 492 392 PciI A'CATG_T Cuts at: 0 1455 1851 Size: 1455 396 PflMI CCAn_nnn'nTGG Cuts at: 0 612 1851 Size: 612 1239 PfoI T'CCnGG_A Cuts at: 0 869 1851 Size: 869 982 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 107 139 1851 Size: 107 32 1712 Fragments arranged by size: 1712 107 32 PsiI TTA'TAA Cuts at: 0 233 1851 Size: 233 1618 PspOMI G'GGCC_C Cuts at: 0 1714 1851 Size: 1714 137 PstI C_TGCA'G Cuts at: 0 963 1613 1851 Size: 963 650 238 Fragments arranged by size: 963 650 238 PvuII CAG'CTG Cuts at: 0 102 1050 1851 Size: 102 948 801 Fragments arranged by size: 948 801 102 SbfI CC_TGCA'GG Cuts at: 0 1613 1851 Size: 1613 238 ScaI AGT'ACT Cuts at: 0 1519 1750 1851 Size: 1519 231 101 Fragments arranged by size: 1519 231 101 SexAI A'CCwGG_T Cuts at: 0 1254 1851 Size: 1254 597 SfcI C'TryA_G Cuts at: 0 959 1609 1851 Size: 959 650 242 Fragments arranged by size: 959 650 242 SmlI C'TyrA_G Cuts at: 0 481 1062 1851 Size: 481 581 789 Fragments arranged by size: 789 581 481 StyI C'CwwG_G Cuts at: 0 243 1390 1851 Size: 243 1147 461 Fragments arranged by size: 1147 461 243 TatI w'GTAC_w Cuts at: 0 179 1517 1748 1851 Size: 179 1338 231 103 Fragments arranged by size: 1338 231 179 103 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1314 1346 1851 Size: 1314 32 505 Fragments arranged by size: 1314 505 32 XbaI T'CTAG_A Cuts at: 0 1417 1851 Size: 1417 434 XmnI GAAnn'nnTTC Cuts at: 0 881 1851 Size: 881 970 Enzymes that do cut: AcuI AflII AflIII AleI AlwNI ApaI ApoI AseI AvaI BanII BbsI BclI BglII Bme1580I BmrI Bpu10I BpuEI BsaBI BsaWI BseRI BseYI BsgI Bsp1286I BspMI BsrBI BsrDI BstEII BstXI BstYI Bsu36I BtgZI BtsI DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII HindIII MmeI MscI MslI MspA1I NdeI NspI PciI PflMI PfoI PpiI PsiI PspOMI PstI PvuII SbfI ScaI SexAI SfcI SmlI StyI TatI TstI XbaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AgeI AhdI AlfI AloI ApaLI AscI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BfrBI BglI BlpI BmgBI BmtI BplI BpmI BsaI BsaAI BsaHI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgI ClaI CspCI DrdI EagI EciI EcoICRI FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PmlI PpuMI PshAI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I XcmI XhoI XmaI ZraI