################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspHI AspI AvaI AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BglI BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiHKAI BsiWI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I FauI FseI HaeII HapII HgaI HindIII HpaII KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 257 GACGTC GACGT^C AciI 94 CCGC CCGC(-3/-1) AcyI 257 GACGTC G[GA]^CG[CT]C AflII 867 CTTAAG C^TTAAG AhdI 856 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 341 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 1091 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 856 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 558 GGACC G^G[AT]CC AviII 123 TGCGCA TGC^GCA BclI 198 TGATCA T^GATCA BfrI 867 CTTAAG C^TTAAG BglII 234 AGATCT A^GATCT BpmI 850 CTGGAG CTGGAG(16/14) BsaHI 257 GACGTC G[GA]^CG[CT]C BsaI 1213 GAGACC GGTCTC(1/5) BseRI 571 GAGGAG GAGGAG(10/8) BsgI 416 GTGCAG GTGCAG(16/14) BsiYI 1330 CCACGAGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 1330 CCACGAGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmBI 1334 GAGACG CGTCTC(1/5) BsmI 922 GAATGC GAATGC(1/-1) BspMI 118 GCAGGT ACCTGC(4/8) BsrI 252 ACTGG ACTGG(1/-1) BssSI 1331 CACGAG CACGAG(-5/-1) BstAPI 115 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 891 GGTTACC G^GT[ACGT]ACC BstPI 891 GGTTACC G^GT[ACGT]ACC CfoI 124 GCGC GCG^C Eam1105I 856 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 891 GGTTACC G^GT[ACGT]ACC EcoRI 1177 GAATTC G^AATTC EcoT22I 492 ATGCAT ATGCA^T FbaI 198 TGATCA T^GATCA FspI 123 TGCGCA TGC^GCA HhaI 124 GCGC GCG^C Hin1I 257 GACGTC G[GA]^CG[CT]C HinP1I 124 GCGC G^CGC HincII 1008 GTTAAC GT[CT]^[GA]AC HindII 1008 GTTAAC GT[CT]^[GA]AC HpaI 1008 GTTAAC GTT^AAC MslI 177 CATAATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1444 CAGCTG C[AC]G^C[GT]G NdeI 214 CATATG CA^TATG NlaIV 1101 GGCTCC GG[ACGT]^[ACGT]CC NsiI 492 ATGCAT ATGCA^T NspI 111 GCATGC [GA]CATG^[CT] PvuII 1444 CAGCTG CAG^CTG SphI 111 GCATGC GCATG^C TaqI 1086 TCGA T^CGA TthHB8I 1086 TCGA T^CGA XbaI 502 TCTAGA T^CTAGA XcmI 1217 CCATCTCAAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1091 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### BsaJI 561 CCTTGG C^C[ACGT][ACGT]GG 595 CCTTGG BsmAI 1213 GAGAC GTCTC(1/5) 1334 GAGAC BsmFI 255 GGGAC GGGAC(10/14) 1075 GTCCC BssKI 61 CCTGG ^CC[ACGT]GG 849 CCTGG BstNI 61 CCTGG CC^[AT]GG 849 CCTGG BstYI 53 GGATCT [GA]^GATC[CT] 234 AGATCT EcoRII 61 CCTGG ^CC[AT]GG 849 CCTGG EcoRV 460 GATATC GAT^ATC 1033 GATATC EcoT14I 561 CCTTGG C^C[AT][AT]GG 595 CCTTGG MaeII 258 ACGT A^CGT 1027 ACGT MflI 53 GGATCT [GA]^GATC[CT] 234 AGATCT MvaI 61 CCTGG CC^[AT]GG 849 CCTGG ScrFI 61 CCTGG CC^[ACGT]GG 849 CCTGG SfaNI 343 GATGC GCATC(5/9) 1123 GATGC StyI 561 CCTTGG C^C[AT][AT]GG 595 CCTTGG XhoII 53 GGATCT [GA]^GATC[CT] 234 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BbsI BbvI BfaI BpuAI BstF5I Cac8I Cfr13I DdeI DpnII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MwoI NdeII NlaIII PleI RsaI Sau3AI Sau96I SfcI SmlI TfiI Tru9I TseI Tsp45I Tsp509I TspRI