(Circular) (Six-base) MAPSORT of: pFN21AB5860.seq Check: 1443 from: 1 to: 6175 pFN21AB5860 6175 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2114 2114 Size: 6175 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2266 3985 278 Size: 53 83 186 936 730 1719 2468 Fragments arranged by size: 2468 1719 936 730 186 83 53 AccI GT'mk_AC Cuts at: 1937 3518 1937 Size: 1581 4594 Acc65I G'GTAC_C Cuts at: 3496 3496 Size: 6175 AclI AA'CG_TT Cuts at: 1563 4302 4675 1563 Size: 2739 373 3063 Fragments arranged by size: 3063 2739 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2432 4238 5622 5890 1632 Size: 800 1806 1384 268 1917 Fragments arranged by size: 1917 1806 1384 800 268 AflII C'TTAA_G Cuts at: 820 1017 2872 820 Size: 197 1855 4123 Fragments arranged by size: 4123 1855 197 AflIII A'CryG_T Cuts at: 1203 5074 6051 1203 Size: 3871 977 1327 Fragments arranged by size: 3871 1327 977 AgeI A'CCGG_T Cuts at: 5006 5006 Size: 6175 AhdI GACnn_n'nnGTC Cuts at: 2866 4904 2866 Size: 2038 4137 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6175 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6175 AlwNI CAG_nnn'CTG Cuts at: 2351 3918 5490 2351 Size: 1567 1572 3036 Fragments arranged by size: 3036 1572 1567 ApaLI G'TGCA_C Cuts at: 4232 5388 4232 Size: 1156 5019 ApoI r'AATT_y Cuts at: 1493 2533 2936 3182 3484 3710 3862 5971 1493 Size: 1040 403 246 302 226 152 2109 1697 Fragments arranged by size: 2109 1697 1040 403 302 246 226 152 AseI AT'TA_AT Cuts at: 160 1032 4729 160 Size: 872 3697 1606 Fragments arranged by size: 3697 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 AvaI C'yCGr_G Cuts at: 1870 1943 3500 1870 Size: 73 1557 4545 Fragments arranged by size: 4545 1557 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2874 2907 2874 Size: 33 6142 BamHI G'GATC_C Cuts at: 3505 3872 3505 Size: 367 5808 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3496 4951 618 Size: 325 217 627 1709 1455 1842 Fragments arranged by size: 1842 1709 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3494 1391 Size: 2103 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2466 2526 3406 928 Size: 971 567 60 880 3697 Fragments arranged by size: 3697 971 880 567 60 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4366 4400 1903 Size: 34 2429 34 3678 Fragments arranged by size: 3678 2429 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4067 5277 4067 Size: 1210 4965 BclI T'GATC_A Cuts at: 1548 2203 1548 Size: 655 5520 BfrBI ATG'CAT Cuts at: 2499 2499 Size: 6175 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4786 136 Size: 107 122 71 631 605 3114 1525 Fragments arranged by size: 3114 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2239 6170 2239 Size: 3931 2244 BlpI GC'TnA_GC Cuts at: 3592 3592 Size: 6175 Bme1580I G_kGCm'C Cuts at: 1790 4236 5392 1790 Size: 2446 1156 2573 Fragments arranged by size: 2573 2446 1156 BmgBI CAC'GTC Cuts at: 1318 3033 1318 Size: 1715 4460 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2267 3906 4864 Size: 416 53 83 399 24 850 1639 958 Cuts at: 4864 442 Size: 1753 Fragments arranged by size: 1753 1639 958 850 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6175 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2917 3241 2917 Size: 324 5851 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2876 4835 1239 Size: 1637 1959 2579 Fragments arranged by size: 2579 1959 1637 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2641 3001 3211 4300 5165 5463 2641 Size: 360 210 1089 865 298 3353 Fragments arranged by size: 3353 1089 865 360 298 210 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3212 4838 882 Size: 617 1713 1626 2219 Fragments arranged by size: 2219 1713 1626 617 BsaAI yAC'GTr Cuts at: 493 1204 3966 493 Size: 711 2762 2702 Fragments arranged by size: 2762 2702 711 BsaBI GATnn'nnATC Cuts at: 6169 6169 Size: 6175 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2263 3982 4364 4999 Size: 53 83 186 936 730 1719 382 635 Cuts at: 4999 275 Size: 1451 Fragments arranged by size: 1719 1451 936 730 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4608 5006 5280 5427 5838 4608 Size: 398 274 147 411 4945 Fragments arranged by size: 4945 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5950 Fragments arranged by size: 5950 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2591 1186 Size: 1405 4770 BseYI C'CCAG_C Cuts at: 3413 5378 5874 3413 Size: 1965 496 3714 Fragments arranged by size: 3714 1965 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2442 1163 Size: 573 706 4896 Fragments arranged by size: 4896 706 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3625 4386 5414 1540 Size: 275 186 1624 761 1028 2301 Fragments arranged by size: 2301 1624 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4236 4321 5392 4236 Size: 85 1071 5019 Fragments arranged by size: 5019 1071 85 BsmI GAATG_Cn' Cuts at: 2933 3686 3779 2933 Size: 753 93 5329 Fragments arranged by size: 5329 753 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3333 3333 Size: 6175 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3494 4236 4321 5392 1391 Size: 399 1704 742 85 1071 2174 Fragments arranged by size: 2174 1704 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5838 5838 Size: 6175 BspHI T'CATG_A Cuts at: 4064 5794 4064 Size: 1730 4445 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2114 3530 844 Size: 1270 1416 3489 Fragments arranged by size: 3489 1416 1270 BsrBI CCG'CTC Cuts at: 1394 1823 4062 1394 Size: 429 2239 3507 Fragments arranged by size: 3507 2239 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4670 4844 65 Size: 1183 3422 174 1396 Fragments arranged by size: 3422 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4819 5006 1700 Size: 110 3009 187 2869 Fragments arranged by size: 3009 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6175 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6175 BssSI C'ACGA_G Cuts at: 3336 4235 5247 3336 Size: 899 1012 4264 Fragments arranged by size: 4264 1012 899 BstAPI GCAn_nnn'nTGC Cuts at: 794 2126 794 Size: 1332 4843 BstBI TT'CG_AA Cuts at: 4979 4979 Size: 6175 BstEII G'GTnAC_C Cuts at: 1184 2896 1184 Size: 1712 4463 BstYI r'GATC_y Cuts at: 1972 2058 2239 3505 3872 4257 4274 5726 5812 Size: 86 181 1266 367 385 17 1452 86 Cuts at: 5812 5824 6170 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1266 385 367 346 181 86 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5623 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5436 Fragments arranged by size: 5436 683 56 BtsI GCAGTG_nn' Cuts at: 2334 2474 2924 3042 3691 4485 4505 2334 Size: 140 450 118 649 794 20 4004 Fragments arranged by size: 4004 794 649 450 140 118 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6175 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6140 DraI TTT'AAA Cuts at: 3479 3479 Size: 6175 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5182 809 Size: 507 3866 1802 Fragments arranged by size: 3866 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3622 4511 6140 Size: 54 1425 50 275 18 1792 889 1629 Cuts at: 6140 8 Size: 43 Fragments arranged by size: 1792 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3622 1537 Size: 275 1810 4090 Fragments arranged by size: 4090 1810 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4105 1353 Size: 2752 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4762 5136 5282 387 Size: 1064 138 3173 374 146 1280 Fragments arranged by size: 3173 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2432 2876 4238 4835 5622 5890 1239 Size: 393 800 444 1362 597 787 268 1524 Fragments arranged by size: 1524 1362 800 787 597 444 393 268 EcoRI G'AATT_C Cuts at: 3182 3484 3862 3182 Size: 302 378 5495 Fragments arranged by size: 5495 378 302 EcoRV GAT'ATC Cuts at: 2467 3040 2467 Size: 573 5602 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2454 2454 Size: 6175 FspI TGC'GCA Cuts at: 2130 4681 2130 Size: 2551 3624 HaeII r_GCGC'y Cuts at: 1678 5322 1678 Size: 3644 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2350 2382 2537 2569 2723 Size: 32 135 32 778 32 155 32 154 Cuts at: 2723 2755 2852 2884 3355 3387 4816 4848 4890 Size: 32 97 32 471 32 1429 32 42 Cuts at: 4890 4922 1373 Size: 32 2626 Fragments arranged by size: 2626 1429 778 471 155 154 135 97 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3015 3519 3765 669 Size: 988 281 1077 504 246 3079 Fragments arranged by size: 3079 1077 988 504 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6175 HpaI GTT'AAC Cuts at: 3015 3765 3015 Size: 750 5425 KpnI G_GTAC'C Cuts at: 3500 3500 Size: 6175 MfeI C'AATT_G Cuts at: 3774 3774 Size: 6175 MluI A'CGCG_T Cuts at: 6051 6051 Size: 6175 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2737 5289 5473 1305 Size: 1432 2552 184 2007 Fragments arranged by size: 2552 2007 1432 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4353 Fragments arranged by size: 4353 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2186 4133 4492 4651 518 Size: 648 1020 1947 359 159 2042 Fragments arranged by size: 2042 1947 1020 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3451 3591 4268 5416 5661 1606 Size: 1845 140 677 1148 245 2120 Fragments arranged by size: 2120 1845 1148 677 245 140 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5623 NdeI CA'TA_TG Cuts at: 387 2220 387 Size: 1833 4342 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6175 NotI GC'GGCC_GC Cuts at: 3622 3622 Size: 6175 NsiI A_TGCA'T Cuts at: 2501 2501 Size: 6175 NspI r_CATG'y Cuts at: 1132 1229 2120 3533 5078 1132 Size: 97 891 1413 1545 2229 Fragments arranged by size: 2229 1545 1413 891 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6175 PciI A'CATG_T Cuts at: 5074 5074 Size: 6175 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6175 PfoI T'CCnGG_A Cuts at: 2065 2065 Size: 6175 PmeI GTTT'AAAC Cuts at: 3479 3479 Size: 6175 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4244 4276 5757 5789 4244 Size: 32 1481 32 4630 Fragments arranged by size: 4630 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6175 PsiI TTA'TAA Cuts at: 3745 3745 Size: 6175 PstI C_TGCA'G Cuts at: 830 3527 830 Size: 2697 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6175 PvuII CAG'CTG Cuts at: 3451 3451 Size: 6175 SalI G'TCGA_C Cuts at: 1936 3517 1936 Size: 1581 4594 SbfI CC_TGCA'GG Cuts at: 3527 3527 Size: 6175 ScaI AGT'ACT Cuts at: 4423 6135 4423 Size: 1712 4463 SfcI C'TryA_G Cuts at: 826 1045 2343 2454 2706 3523 4658 5339 5530 Size: 219 1298 111 252 817 1135 681 191 Cuts at: 5530 826 Size: 1471 Fragments arranged by size: 1471 1298 1135 817 681 252 219 191 111 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6175 SmaI CCC'GGG Cuts at: 1872 3502 1872 Size: 1630 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2620 2872 2980 3226 4279 5180 Size: 197 926 677 252 108 246 1053 901 Cuts at: 5180 5442 820 Size: 262 1553 Fragments arranged by size: 1553 1053 926 901 677 262 252 246 197 108 SnaBI TAC'GTA Cuts at: 493 3966 493 Size: 3473 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6175 SphI G_CATG'C Cuts at: 1132 2120 3533 1132 Size: 988 1413 3774 Fragments arranged by size: 3774 1413 988 SspI AAT'ATT Cuts at: 5 52 4099 5 Size: 47 4047 2081 Fragments arranged by size: 4047 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6175 StyI C'CwwG_G Cuts at: 513 1065 2566 2600 3614 513 Size: 552 1501 34 1014 3074 Fragments arranged by size: 3074 1501 1014 552 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4202 4219 4372 4557 5002 325 Size: 1255 2622 17 153 185 445 1498 Fragments arranged by size: 2622 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2178 2921 4421 Size: 274 80 33 51 1443 201 743 1500 Cuts at: 4421 6133 96 Size: 1712 138 Fragments arranged by size: 1712 1500 1443 743 274 201 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2800 2832 6135 6167 2800 Size: 32 3303 32 2808 Fragments arranged by size: 3303 2808 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6175 XbaI T'CTAG_A Cuts at: 2507 3511 2507 Size: 1004 5171 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3229 3229 Size: 6175 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6175 XmaI C'CCGG_G Cuts at: 1870 3500 1870 Size: 1630 4545 XmnI GAAnn'nnTTC Cuts at: 3100 4304 3100 Size: 1204 4971 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2264 3983 276 Size: 53 83 186 936 730 1719 2468 Fragments arranged by size: 2468 1719 936 730 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI