################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AgeI AhdI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BalI BamHI BbrPI BbsI BcgI BciVI BclI BglII BlnI BpuAI BsaBI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI Eam1105I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI HincII HindII HindIII HpaI KpnI KspI MaeIII MamI MfeI MluNI MscI MunI NcoI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StyI SwaI TfiI Tsp45I Tsp509I Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccIII 813 TCCGGA T^CCGGA AfaI 2068 GTAC GT^AC AflII 949 CTTAAG C^TTAAG AflIII 2045 ACGCGT A^C[GA][CT]GT AlwI 72 GGATC GGATC(4/5) AlwNI 779 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1276 CCTGAGG CC^T[ACGT]AGG Asp700I 189 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BfrI 949 CTTAAG C^TTAAG BsaAI 335 CACGTA [CT]AC^GT[GA] BsaI 2080 GAGACC GGTCTC(1/5) BseAI 813 TCCGGA T^CCGGA BsmBI 1739 CGTCTC CGTCTC(1/5) BspEI 813 TCCGGA T^CCGGA BstAPI 546 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 974 CCAGCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 72 GGATCT [GA]^GATC[CT] Bsu36I 1276 CCTGAGG CC^T[ACGT]AGG DraIII 989 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 633 CCACGG C^C[GA][CT]GG Eco81I 1276 CCTGAGG CC^T[ACGT]AGG HinfI 147 GAGTC G^A[ACGT]TC MflI 72 GGATCT [GA]^GATC[CT] MluI 2045 ACGCGT A^CGCGT MroI 813 TCCGGA T^CCGGA MseI 950 TTAA T^TAA MslI 960 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NotI 920 GCGGCCGC GC^GGCCGC PleI 147 GAGTC GAGTC(4/5) PstI 76 CTGCAG CTGCA^G RsaI 2068 GTAC GT^AC SacI 1975 GAGCTC GAGCT^C SapI 370 GAAGAGC GCTCTTC(1/4) SfaNI 1545 GCATC GCATC(5/9) SphI 425 GCATGC GCATG^C Tru9I 950 TTAA T^TAA XcmI 443 CCACTGAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 72 GGATCT [GA]^GATC[CT] XmnI 189 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 1480 GGCGCC G[GA]^CG[CT]C 1768 GGCGCC Aor51HI 191 AGCGCT AGC^GCT 1077 AGCGCT AviII 311 TGCGCA TGC^GCA 1847 TGCGCA BbeI 1480 GGCGCC GGCGC^C 1768 GGCGCC BfaI 1793 CTAG C^TAG 2018 CTAG BglI 631 GCCCACGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1130 GCCTCTTGGGC BlpI 1186 GCTGAGC GC^T[ACGT]AGC 1523 GCTCAGC Bpu1102I 1186 GCTGAGC GC^T[ACGT]AGC 1523 GCTCAGC BsaHI 1480 GGCGCC G[GA]^CG[CT]C 1768 GGCGCC BsaWI 813 TCCGGA [AT]^CCGG[AT] 912 TCCGGT BsmAI 2080 GAGAC GTCTC(1/5) 1740 GTCTC BssHII 375 GCGCGC G^CGCGC 377 GCGCGC BstF5I 549 CATCC GGATG(2/0) 122 GGATG CelII 1186 GCTGAGC GC^T[ACGT]AGC 1523 GCTCAGC Eco47III 191 AGCGCT AGC^GCT 1077 AGCGCT FokI 549 CATCC GGATG(9/13) 122 GGATG FseI 1165 GGCCGGCC GGCCGG^CC 1665 GGCCGGCC FspI 311 TGCGCA TGC^GCA 1847 TGCGCA Hin1I 1480 GGCGCC G[GA]^CG[CT]C 1768 GGCGCC KasI 1480 GGCGCC G^GCGCC 1768 GGCGCC MaeI 1793 CTAG C^TAG 2018 CTAG NarI 1480 GGCGCC GG^CGCC 1768 GGCGCC NspI 425 GCATGC [GA]CATG^[CT] 875 ACATGC SfcI 76 CTGCAG C^T[GA][CT]AG 1633 CTACAG SfoI 1480 GGCGCC GGC^GCC 1768 GGCGCC StuI 248 AGGCCT AGG^CCT 890 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AccII AciI AluI Alw44I ApaI ApaLI AspHI AvaI AvaII BanI BanII BbvCI BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI Ksp632I MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PpuMI Sau3AI Sau96I ScrFI SmaI SmlI TaqI TseI TspRI TthHB8I XmaCI XmaI