(Circular) (Six-base) MAPSORT of: pFN21AB6210.seq Check: 6121 from: 1 to: 6871 pFN21AB6210 6871 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2238 2322 2556 2238 Size: 84 234 6553 Fragments arranged by size: 6553 234 84 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4681 278 Size: 53 83 186 936 3145 2468 Fragments arranged by size: 3145 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4214 1937 Size: 2277 4594 Acc65I G'GTAC_C Cuts at: 4192 4192 Size: 6871 AclI AA'CG_TT Cuts at: 1563 4998 5371 1563 Size: 3435 373 3063 Fragments arranged by size: 3435 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3492 4934 6318 6586 1632 Size: 1860 1442 1384 268 1917 Fragments arranged by size: 1917 1860 1442 1384 268 AfeI AGC'GCT Cuts at: 2198 3084 2198 Size: 886 5985 AflII C'TTAA_G Cuts at: 820 1017 2954 820 Size: 197 1937 4737 Fragments arranged by size: 4737 1937 197 AflIII A'CryG_T Cuts at: 1203 4050 5770 6747 1203 Size: 2847 1720 977 1327 Fragments arranged by size: 2847 1720 1327 977 AgeI A'CCGG_T Cuts at: 5702 5702 Size: 6871 AhdI GACnn_n'nnGTC Cuts at: 5600 5600 Size: 6871 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6871 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6871 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2157 2189 2933 2965 2157 Size: 32 744 32 6063 Fragments arranged by size: 6063 744 32 32 AlwNI CAG_nnn'CTG Cuts at: 2789 4614 6186 2789 Size: 1825 1572 3474 Fragments arranged by size: 3474 1825 1572 ApaI G_GGCC'C Cuts at: 2993 3080 3328 3412 2993 Size: 87 248 84 6452 Fragments arranged by size: 6452 248 87 84 ApaLI G'TGCA_C Cuts at: 2972 3560 4004 4928 6084 2972 Size: 588 444 924 1156 3759 Fragments arranged by size: 3759 1156 924 588 444 ApoI r'AATT_y Cuts at: 1493 4180 4406 4558 6667 1493 Size: 2687 226 152 2109 1697 Fragments arranged by size: 2687 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5425 160 Size: 872 4393 1606 Fragments arranged by size: 4393 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6871 AvaI C'yCGr_G Cuts at: 1870 1943 2029 2123 2330 2648 2798 3004 3130 Size: 73 86 94 207 318 150 206 126 Cuts at: 3130 3252 3327 3370 3677 4068 4196 1870 Size: 122 75 43 307 391 128 4545 Fragments arranged by size: 4545 391 318 307 207 206 150 128 126 122 94 86 75 73 43 BamHI G'GATC_C Cuts at: 4201 4568 4201 Size: 367 6504 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3264 3485 3608 3773 3932 Size: 325 217 627 1477 221 123 165 159 Cuts at: 3932 4192 5647 618 Size: 260 1455 1842 Fragments arranged by size: 1842 1477 1455 627 325 260 221 217 165 159 123 BanII G_rGCy'C Cuts at: 1391 2015 2251 2299 2416 2647 2760 2808 2946 Size: 624 236 48 117 231 113 48 138 Cuts at: 2946 2993 3080 3129 3198 3259 3328 3347 3412 Size: 47 87 49 69 61 69 19 65 Cuts at: 3412 3976 3984 4124 4190 1391 Size: 564 8 140 66 4072 Fragments arranged by size: 4072 624 564 236 231 140 138 117 113 87 69 69 66 65 61 49 48 48 47 19 8 BbeI G_GCGC'C Cuts at: 3489 3777 3489 Size: 288 6583 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5900 BbvCI CC'TCA_GC Cuts at: 2688 2694 2700 2688 Size: 6 6 6859 Fragments arranged by size: 6859 6 6 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5062 5096 1903 Size: 34 3125 34 3678 Fragments arranged by size: 3678 3125 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4763 5973 4763 Size: 1210 5661 BclI T'GATC_A Cuts at: 1548 1548 Size: 6871 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2642 3141 5482 Size: 107 122 71 631 605 970 499 2341 Cuts at: 5482 136 Size: 1525 Fragments arranged by size: 2341 1525 970 631 605 499 122 107 71 BglII A'GATC_T Cuts at: 6866 6866 Size: 6871 BlpI GC'TnA_GC Cuts at: 3192 3529 4288 3192 Size: 337 759 5775 Fragments arranged by size: 5775 759 337 Bme1580I G_kGCm'C Cuts at: 1790 2267 2976 2993 3004 3080 3328 3390 3412 Size: 477 709 17 11 76 248 62 22 Cuts at: 3412 3564 3611 3937 4008 4932 6088 1790 Size: 152 47 326 71 924 1156 2573 Fragments arranged by size: 2573 1156 924 709 477 326 248 152 76 71 62 47 22 17 11 BmgBI CAC'GTC Cuts at: 1318 2018 1318 Size: 700 6171 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2414 3607 4089 Size: 416 53 83 399 24 997 1193 482 Cuts at: 4089 4602 5560 442 Size: 513 958 1753 Fragments arranged by size: 1753 1193 997 958 513 482 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6871 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3793 4138 3793 Size: 345 6526 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2387 2581 3753 3990 5531 1239 Size: 1148 194 1172 237 1541 2579 Fragments arranged by size: 2579 1541 1172 1148 237 194 Bpu10I CC'TnA_GC Cuts at: 2422 2688 2694 2700 3149 2422 Size: 266 6 6 449 6144 Fragments arranged by size: 6144 449 266 6 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2855 3611 3763 4996 5861 6159 2855 Size: 756 152 1233 865 298 3567 Fragments arranged by size: 3567 1233 865 756 298 152 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4079 5534 882 Size: 617 2580 1455 2219 Fragments arranged by size: 2580 2219 1455 617 BsaAI yAC'GTr Cuts at: 493 1204 2342 4662 493 Size: 711 1138 2320 2702 Fragments arranged by size: 2702 2320 1138 711 BsaBI GATnn'nnATC Cuts at: 6865 6865 Size: 6871 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3486 3774 4678 5060 Size: 53 83 186 936 1953 288 904 382 Cuts at: 5060 5695 275 Size: 635 1451 Fragments arranged by size: 1953 1451 936 904 635 382 288 186 83 53 BsaWI w'CCGG_w Cuts at: 2818 2917 5304 5702 5976 6123 6534 2818 Size: 99 2387 398 274 147 411 3155 Fragments arranged by size: 3155 2387 411 398 274 147 99 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2770 2800 2882 2912 2933 Size: 30 165 30 1367 30 82 30 21 Cuts at: 2933 2963 1178 Size: 30 5086 Fragments arranged by size: 5086 1367 165 82 30 30 30 30 30 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2621 2624 2816 3077 3290 3308 3326 3676 Size: 1435 3 192 261 213 18 18 350 Cuts at: 3676 4130 1186 Size: 454 3927 Fragments arranged by size: 3927 1435 454 350 261 213 192 18 18 3 BseYI C'CCAG_C Cuts at: 2712 2736 2809 2986 3036 3079 3340 3404 3708 Size: 24 73 177 50 43 261 64 304 Cuts at: 3708 3806 4153 6074 6570 2712 Size: 98 347 1921 496 3013 Fragments arranged by size: 3013 1921 496 347 304 261 177 98 73 64 50 43 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2178 2337 2534 2603 2605 3344 1163 Size: 573 442 159 197 69 2 739 4690 Fragments arranged by size: 4690 739 573 442 197 159 69 2 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2929 3114 3208 3426 4321 5082 Size: 275 186 928 185 94 218 895 761 Cuts at: 5082 6110 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 928 895 761 275 218 186 185 94 BsiHKAI G_wGCw'C Cuts at: 2976 3564 3984 4008 4932 5017 6088 2976 Size: 588 420 24 924 85 1071 3759 Fragments arranged by size: 3759 1071 924 588 420 85 24 BsmI GAATG_Cn' Cuts at: 4382 4475 4382 Size: 93 6778 BsmBI CGTCTCn'nnnn_ Cuts at: 3750 3750 Size: 6871 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2251 2267 2299 2416 2647 2760 Size: 399 225 236 16 32 117 231 113 Cuts at: 2760 2808 2946 2976 2993 3004 3080 3129 3198 Size: 48 138 30 17 11 76 49 69 Cuts at: 3198 3259 3328 3347 3390 3412 3564 3611 3937 Size: 61 69 19 43 22 152 47 326 Cuts at: 3937 3976 3984 4008 4124 4190 4932 5017 6088 Size: 39 8 24 116 66 742 85 1071 Cuts at: 6088 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 326 236 231 225 152 138 117 116 113 85 76 69 69 66 61 49 48 47 43 39 32 30 24 22 19 17 16 11 8 BspEI T'CCGG_A Cuts at: 2818 6534 2818 Size: 3716 3155 BspHI T'CATG_A Cuts at: 4760 6490 4760 Size: 1730 5141 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2238 2322 2556 3324 4226 844 Size: 1394 84 234 768 902 3489 Fragments arranged by size: 3489 1394 902 768 234 84 BsrBI CCG'CTC Cuts at: 1394 1823 2034 2135 2795 3528 3574 3666 4758 Size: 429 211 101 660 733 46 92 1092 Cuts at: 4758 1394 Size: 3507 Fragments arranged by size: 3507 1092 733 660 429 211 101 92 46 BsrDI GCAATG_nn' Cuts at: 65 1248 5366 5540 65 Size: 1183 4118 174 1396 Fragments arranged by size: 4118 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2056 3171 3261 3491 3671 4032 5515 Size: 110 246 1115 90 230 180 361 1483 Cuts at: 5515 5702 1700 Size: 187 2869 Fragments arranged by size: 2869 1483 1115 361 246 230 187 180 110 90 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6871 BssHII G'CGCG_C Cuts at: 1925 2380 2382 1925 Size: 455 2 6414 Fragments arranged by size: 6414 455 2 BssSI C'ACGA_G Cuts at: 4931 5943 4931 Size: 1012 5859 BstAPI GCAn_nnn'nTGC Cuts at: 794 2557 794 Size: 1763 5108 BstBI TT'CG_AA Cuts at: 5675 5675 Size: 6871 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6871 BstXI CCAn_nnnn'nTGG Cuts at: 2986 2986 Size: 6871 BstYI r'GATC_y Cuts at: 1972 2077 4201 4568 4953 4970 6422 6508 6520 Size: 105 2124 367 385 17 1452 86 12 Cuts at: 6520 6866 1972 Size: 346 1977 Fragments arranged by size: 2124 1977 1452 385 367 346 105 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3282 3282 Size: 6871 BtgI C'CryG_G Cuts at: 513 1065 2004 2638 513 Size: 552 939 634 4746 Fragments arranged by size: 4746 939 634 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6132 Fragments arranged by size: 6132 683 56 BtsI GCAGTG_nn' Cuts at: 2269 2993 3592 4387 5181 5201 2269 Size: 724 599 795 794 20 3939 Fragments arranged by size: 3939 795 794 724 599 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6871 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6836 DraI TTT'AAA Cuts at: 4175 4175 Size: 6871 DraIII CAC_nnn'GTG Cuts at: 2999 2999 Size: 6871 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5878 809 Size: 507 4562 1802 Fragments arranged by size: 4562 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2035 2275 2664 Size: 54 1425 50 275 18 205 240 389 Cuts at: 2664 2842 2926 3111 3205 4034 4318 5207 6836 Size: 178 84 185 94 829 284 889 1629 Cuts at: 6836 8 Size: 43 Fragments arranged by size: 1629 1425 889 829 389 284 275 240 205 185 178 94 84 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2926 3111 3205 4318 1537 Size: 275 1114 185 94 1113 4090 Fragments arranged by size: 4090 1114 1113 275 185 94 EarI CTCTTCn'nnn_ Cuts at: 1353 2370 3285 3432 3956 4801 1353 Size: 1017 915 147 524 845 3423 Fragments arranged by size: 3423 1017 915 845 524 147 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2476 2546 2673 3215 5458 5832 Size: 1064 138 887 70 127 542 2243 374 Cuts at: 5832 5978 387 Size: 146 1280 Fragments arranged by size: 2243 1280 1064 887 542 374 146 138 127 70 EcoICRI GAG'CTC Cuts at: 3982 3982 Size: 6871 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2387 2581 3492 3753 3990 4934 5531 Size: 393 755 194 911 261 237 944 597 Cuts at: 5531 6318 6586 1239 Size: 787 268 1524 Fragments arranged by size: 1524 944 911 787 755 597 393 268 261 237 194 EcoO109I rG'GnC_Cy Cuts at: 2565 3008 3408 3462 3816 3846 2565 Size: 443 400 54 354 30 5590 Fragments arranged by size: 5590 443 400 354 54 30 EcoRI G'AATT_C Cuts at: 4180 4558 4180 Size: 378 6493 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2917 2917 Size: 6871 FseI GG_CCGG'CC Cuts at: 3175 3675 3175 Size: 500 6371 FspI TGC'GCA Cuts at: 2318 3854 5377 2318 Size: 1536 1523 3812 Fragments arranged by size: 3812 1536 1523 HaeII r_GCGC'y Cuts at: 1678 2048 2173 2200 2338 2816 3086 3121 3489 Size: 370 125 27 138 478 270 35 368 Cuts at: 3489 3646 3777 6018 1678 Size: 157 131 2241 2531 Fragments arranged by size: 2531 2241 478 370 368 270 157 138 131 125 35 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2770 2802 5512 5544 5586 Size: 32 135 32 1198 32 2710 32 42 Cuts at: 5586 5618 1373 Size: 32 2626 Fragments arranged by size: 2710 2626 1198 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4215 4461 669 Size: 988 281 2277 246 3079 Fragments arranged by size: 3079 2277 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6871 HpaI GTT'AAC Cuts at: 4461 4461 Size: 6871 KasI G'GCGC_C Cuts at: 3485 3773 3485 Size: 288 6583 KpnI G_GTAC'C Cuts at: 4196 4196 Size: 6871 MfeI C'AATT_G Cuts at: 4470 4470 Size: 6871 MluI A'CGCG_T Cuts at: 4050 6747 4050 Size: 2697 4174 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2118 2352 3436 3803 5985 6169 1305 Size: 813 234 1084 367 2182 184 2007 Fragments arranged by size: 2182 2007 1084 813 367 234 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5049 Fragments arranged by size: 5049 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2969 4829 5188 5347 518 Size: 648 1803 1860 359 159 2042 Fragments arranged by size: 2042 1860 1803 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2293 2675 2829 2931 3232 3712 3945 4287 Size: 687 382 154 102 301 480 233 342 Cuts at: 4287 4964 6112 6357 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 687 677 480 382 342 301 245 233 154 102 NaeI GCC'GGC Cuts at: 2058 3173 3263 3673 4034 2058 Size: 1115 90 410 361 4895 Fragments arranged by size: 4895 1115 410 361 90 NarI GG'CG_CC Cuts at: 3486 3774 3486 Size: 288 6583 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5380 Fragments arranged by size: 5380 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6871 NgoMIV G'CCGG_C Cuts at: 2056 3171 3261 3671 4032 2056 Size: 1115 90 410 361 4895 Fragments arranged by size: 4895 1115 410 361 90 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6871 NotI GC'GGCC_GC Cuts at: 2926 4318 2926 Size: 1392 5479 NspI r_CATG'y Cuts at: 1132 1229 2434 2884 4229 5774 1132 Size: 97 1205 450 1345 1545 2229 Fragments arranged by size: 2229 1545 1345 1205 450 97 PasI CC'CwG_GG Cuts at: 1348 3839 1348 Size: 2491 4380 PciI A'CATG_T Cuts at: 5770 5770 Size: 6871 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6871 PmeI GTTT'AAAC Cuts at: 4175 4175 Size: 6871 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2933 2965 4940 4972 6453 6485 2933 Size: 32 1975 32 1481 32 3319 Fragments arranged by size: 3319 1975 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3462 3816 3846 3462 Size: 354 30 6487 Fragments arranged by size: 6487 354 30 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6871 PsiI TTA'TAA Cuts at: 4441 4441 Size: 6871 PspOMI G'GGCC_C Cuts at: 2989 3076 3324 3408 2989 Size: 87 248 84 6452 Fragments arranged by size: 6452 248 87 84 PstI C_TGCA'G Cuts at: 830 2085 4223 830 Size: 1255 2138 3478 Fragments arranged by size: 3478 2138 1255 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6871 SacI G_AGCT'C Cuts at: 3984 3984 Size: 6871 SalI G'TCGA_C Cuts at: 1936 4213 1936 Size: 2277 4594 SanDI GG'GwC_CC Cuts at: 3462 3816 3462 Size: 354 6517 SapI GCTCTTCn'nnn_ Cuts at: 2370 2370 Size: 6871 SbfI CC_TGCA'GG Cuts at: 4223 4223 Size: 6871 ScaI AGT'ACT Cuts at: 5119 6831 5119 Size: 1712 5159 SfcI C'TryA_G Cuts at: 826 1045 2081 3638 4219 5354 6035 6226 826 Size: 219 1036 1557 581 1135 681 191 1471 Fragments arranged by size: 1557 1471 1135 1036 681 581 219 191 SfoI GGC'GCC Cuts at: 3487 3775 3487 Size: 288 6583 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6871 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6871 SmaI CCC'GGG Cuts at: 1872 3006 3254 3329 3372 3679 4198 1872 Size: 1134 248 75 43 307 519 4545 Fragments arranged by size: 4545 1134 519 307 248 75 43 SmlI C'TyrA_G Cuts at: 820 1017 1943 2870 2954 3626 3778 4975 5876 Size: 197 926 927 84 672 152 1197 901 Cuts at: 5876 6138 820 Size: 262 1553 Fragments arranged by size: 1553 1197 927 926 901 672 262 197 152 84 SnaBI TAC'GTA Cuts at: 493 4662 493 Size: 4169 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6871 SphI G_CATG'C Cuts at: 1132 2434 4229 1132 Size: 1302 1795 3774 Fragments arranged by size: 3774 1795 1302 SrfI GCCC'GGGC Cuts at: 3254 3254 Size: 6871 SspI AAT'ATT Cuts at: 5 52 4795 5 Size: 47 4743 2081 Fragments arranged by size: 4743 2081 47 StuI AGG'CCT Cuts at: 1517 2255 2897 1517 Size: 738 642 5491 Fragments arranged by size: 5491 738 642 StyI C'CwwG_G Cuts at: 513 1065 2004 4310 513 Size: 552 939 2306 3074 Fragments arranged by size: 3074 2306 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2256 3626 3754 4898 4915 5068 5253 Size: 1255 676 1370 128 1144 17 153 185 Cuts at: 5253 5698 325 Size: 445 1498 Fragments arranged by size: 1498 1370 1255 1144 676 445 185 153 128 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5117 6829 96 Size: 274 80 33 51 1443 3140 1712 138 Fragments arranged by size: 3140 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2882 2914 6831 6863 2882 Size: 32 3917 32 2890 Fragments arranged by size: 3917 2890 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6871 XbaI T'CTAG_A Cuts at: 4207 4207 Size: 6871 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2455 2455 Size: 6871 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6871 XmaI C'CCGG_G Cuts at: 1870 3004 3252 3327 3370 3677 4196 1870 Size: 1134 248 75 43 307 519 4545 Fragments arranged by size: 4545 1134 519 307 248 75 43 XmnI GAAnn'nnTTC Cuts at: 2198 5000 2198 Size: 2802 4069 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4679 276 Size: 53 83 186 936 3145 2468 Fragments arranged by size: 3145 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoNI EcoRV FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SexAI SfiI SwaI