(Circular) (Six-base) MAPSORT of: pFN21AE9629.seq Check: 3456 from: 1 to: 7708 pFN21AE9629 7708 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2509 2509 Size: 7708 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5518 278 Size: 53 83 186 936 3982 2468 Fragments arranged by size: 3982 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2385 5051 1937 Size: 448 2666 4594 Fragments arranged by size: 4594 2666 448 Acc65I G'GTAC_C Cuts at: 5029 5029 Size: 7708 AclI AA'CG_TT Cuts at: 1563 5835 6208 1563 Size: 4272 373 3063 Fragments arranged by size: 4272 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2250 2666 3800 5771 7155 7423 1632 Size: 618 416 1134 1971 1384 268 1917 Fragments arranged by size: 1971 1917 1384 1134 618 416 268 AflII C'TTAA_G Cuts at: 820 1017 4004 820 Size: 197 2987 4524 Fragments arranged by size: 4524 2987 197 AflIII A'CryG_T Cuts at: 1203 2490 4766 6607 7584 1203 Size: 1287 2276 1841 977 1327 Fragments arranged by size: 2276 1841 1327 1287 977 AgeI A'CCGG_T Cuts at: 6539 6539 Size: 7708 AhdI GACnn_n'nnGTC Cuts at: 2161 4927 6437 2161 Size: 2766 1510 3432 Fragments arranged by size: 3432 2766 1510 AleI CACnn'nnGTG Cuts at: 1166 3462 4647 4767 1166 Size: 2296 1185 120 4107 Fragments arranged by size: 4107 2296 1185 120 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7708 AlwNI CAG_nnn'CTG Cuts at: 2273 2431 2632 2674 3301 4318 5451 7023 2273 Size: 158 201 42 627 1017 1133 1572 2958 Fragments arranged by size: 2958 1572 1133 1017 627 201 158 42 ApaI G_GGCC'C Cuts at: 4271 4490 4271 Size: 219 7489 ApaLI G'TGCA_C Cuts at: 5765 6921 5765 Size: 1156 6552 ApoI r'AATT_y Cuts at: 1493 2201 2219 2377 2849 3809 5017 5243 5395 Size: 708 18 158 472 960 1208 226 152 Cuts at: 5395 7504 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1208 960 708 472 226 158 152 18 AseI AT'TA_AT Cuts at: 160 1032 6262 160 Size: 872 5230 1606 Fragments arranged by size: 5230 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7708 AvaI C'yCGr_G Cuts at: 1870 1943 4905 5033 1870 Size: 73 2962 128 4545 Fragments arranged by size: 4545 2962 128 73 AvrII C'CTAG_G Cuts at: 4645 4645 Size: 7708 BamHI G'GATC_C Cuts at: 2920 3494 4638 5038 5405 2920 Size: 574 1144 400 367 5223 Fragments arranged by size: 5223 1144 574 400 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3690 4607 4752 5029 6484 Size: 325 217 627 1903 917 145 277 1455 Cuts at: 6484 618 Size: 1842 Fragments arranged by size: 1903 1842 1455 917 627 325 277 217 145 BanII G_rGCy'C Cuts at: 1391 2533 3966 4271 4490 4846 5027 1391 Size: 1142 1433 305 219 356 181 4072 Fragments arranged by size: 4072 1433 1142 356 305 219 181 BbeI G_GCGC'C Cuts at: 4611 4756 4611 Size: 145 7563 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2061 928 Size: 971 162 6575 Fragments arranged by size: 6575 971 162 BbvCI CC'TCA_GC Cuts at: 4034 4034 Size: 7708 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2042 2076 3557 3591 5899 5933 1903 Size: 34 105 34 1481 34 2308 34 3678 Fragments arranged by size: 3678 2308 1481 105 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3456 5600 6810 3456 Size: 2144 1210 4354 Fragments arranged by size: 4354 2144 1210 BclI T'GATC_A Cuts at: 1548 2593 3454 1548 Size: 1045 861 5802 Fragments arranged by size: 5802 1045 861 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2783 4629 6319 Size: 107 122 71 631 605 1111 1846 1690 Cuts at: 6319 136 Size: 1525 Fragments arranged by size: 1846 1690 1525 1111 631 605 122 107 71 BglII A'GATC_T Cuts at: 2211 2948 7703 2211 Size: 737 4755 2216 Fragments arranged by size: 4755 2216 737 BlpI GC'TnA_GC Cuts at: 4063 5125 4063 Size: 1062 6646 Bme1580I G_kGCm'C Cuts at: 1790 3693 3980 4271 4490 5769 6925 1790 Size: 1903 287 291 219 1279 1156 2573 Fragments arranged by size: 2573 1903 1279 1156 291 287 219 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7708 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2759 3895 4380 Size: 416 53 83 399 24 1342 1136 485 Cuts at: 4380 5439 6397 442 Size: 1059 958 1753 Fragments arranged by size: 1753 1342 1136 1059 958 485 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4284 1056 Size: 3228 4480 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4078 4078 Size: 7708 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3481 4112 4217 4812 4860 4898 6368 1239 Size: 2242 631 105 595 48 38 1470 2579 Fragments arranged by size: 2579 2242 1470 631 595 105 48 38 Bpu10I CC'TnA_GC Cuts at: 3351 3387 4034 3351 Size: 36 647 7025 Fragments arranged by size: 7025 647 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2961 3020 3057 5833 6698 6996 2961 Size: 59 37 2776 865 298 3673 Fragments arranged by size: 3673 2776 865 298 59 37 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3356 3530 3626 6371 882 Size: 617 1857 174 96 2745 2219 Fragments arranged by size: 2745 2219 1857 617 174 96 BsaAI yAC'GTr Cuts at: 493 1204 2491 3655 5499 493 Size: 711 1287 1164 1844 2702 Fragments arranged by size: 2702 1844 1287 1164 711 BsaBI GATnn'nnATC Cuts at: 2229 3380 3444 7702 2229 Size: 1151 64 4258 2235 Fragments arranged by size: 4258 2235 1151 64 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4608 4753 5515 5897 Size: 53 83 186 936 3075 145 762 382 Cuts at: 5897 6532 275 Size: 635 1451 Fragments arranged by size: 3075 1451 936 762 635 382 186 145 83 53 BsaWI w'CCGG_w Cuts at: 2034 6141 6539 6813 6960 7371 2034 Size: 4107 398 274 147 411 2371 Fragments arranged by size: 4107 2371 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7483 Fragments arranged by size: 7483 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7708 BseYI C'CCAG_C Cuts at: 3168 3509 3930 3949 4347 6911 7407 3168 Size: 341 421 19 398 2564 496 3469 Fragments arranged by size: 3469 2564 496 421 398 341 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3854 4748 1163 Size: 573 2118 894 4123 Fragments arranged by size: 4123 2118 894 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2173 5158 5919 6947 1540 Size: 275 186 172 2985 761 1028 2301 Fragments arranged by size: 2985 2301 1028 761 275 186 172 BsiHKAI G_wGCw'C Cuts at: 3627 5769 5854 6925 3627 Size: 2142 85 1071 4410 Fragments arranged by size: 4410 2142 1071 85 BsmI GAATG_Cn' Cuts at: 3189 5219 5312 3189 Size: 2030 93 5585 Fragments arranged by size: 5585 2030 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2533 3627 3693 3966 3980 4271 4490 Size: 399 743 1094 66 273 14 291 219 Cuts at: 4490 4846 5027 5769 5854 6925 1391 Size: 356 181 742 85 1071 2174 Fragments arranged by size: 2174 1094 1071 743 742 399 356 291 273 219 181 85 66 14 BspEI T'CCGG_A Cuts at: 7371 7371 Size: 7708 BspHI T'CATG_A Cuts at: 2290 2824 3414 5597 7327 2290 Size: 534 590 2183 1730 2671 Fragments arranged by size: 2671 2183 1730 590 534 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2166 2509 3838 4448 5063 844 Size: 1322 343 1329 610 615 3489 Fragments arranged by size: 3489 1329 1322 615 610 343 BsrBI CCG'CTC Cuts at: 1394 1823 2767 3199 4826 5595 1394 Size: 429 944 432 1627 769 3507 Fragments arranged by size: 3507 1627 944 769 432 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3226 3573 6203 6377 65 Size: 1183 1978 347 2630 174 1396 Fragments arranged by size: 2630 1978 1396 1183 347 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4500 6352 6539 1700 Size: 110 2690 1852 187 2869 Fragments arranged by size: 2869 2690 1852 187 110 BsrGI T'GTAC_A Cuts at: 96 4143 96 Size: 4047 3661 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7708 BssSI C'ACGA_G Cuts at: 5768 6780 5768 Size: 1012 6696 BstAPI GCAn_nnn'nTGC Cuts at: 794 2524 3832 4692 4962 794 Size: 1730 1308 860 270 3540 Fragments arranged by size: 3540 1730 1308 860 270 BstBI TT'CG_AA Cuts at: 2311 6512 2311 Size: 4201 3507 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7708 BstXI CCAn_nnnn'nTGG Cuts at: 2086 2837 3517 3908 4195 4292 4649 4973 2086 Size: 751 680 391 287 97 357 324 4821 Fragments arranged by size: 4821 751 680 391 357 324 287 97 BstYI r'GATC_y Cuts at: 1972 2211 2920 2948 3471 3494 4242 4638 5038 Size: 239 709 28 523 23 748 396 400 Cuts at: 5038 5405 5790 5807 7259 7345 7357 7703 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 748 709 523 400 396 385 367 346 239 86 28 23 17 12 Bsu36I CC'TnA_GG Cuts at: 2639 2697 4235 2639 Size: 58 1538 6112 Fragments arranged by size: 6112 1538 58 BtgI C'CryG_G Cuts at: 513 1065 2079 2253 2925 3406 3516 3726 4410 Size: 552 1014 174 672 481 110 210 684 Cuts at: 4410 513 Size: 3811 Fragments arranged by size: 3811 1014 684 672 552 481 210 174 110 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2622 3367 487 Size: 683 56 1396 745 4828 Fragments arranged by size: 4828 1396 745 683 56 BtsI GCAGTG_nn' Cuts at: 4117 4681 4796 5224 6018 6038 4117 Size: 564 115 428 794 20 5787 Fragments arranged by size: 5787 794 564 428 115 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7708 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2870 2905 529 Size: 35 2306 35 5332 Fragments arranged by size: 5332 2306 35 35 DraI TTT'AAA Cuts at: 5012 5012 Size: 7708 DraIII CAC_nnn'GTG Cuts at: 4141 4141 Size: 7708 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4983 6715 809 Size: 507 3667 1732 1802 Fragments arranged by size: 3667 1802 1732 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 2139 2239 Size: 54 1425 50 275 18 291 18 100 Cuts at: 2239 4294 4450 5155 6044 7673 8 Size: 2055 156 705 889 1629 43 Fragments arranged by size: 2055 1629 1425 889 705 291 275 156 100 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 5155 1537 Size: 275 3343 4090 Fragments arranged by size: 4090 3343 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5638 1353 Size: 4285 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2197 4233 6295 6669 6815 387 Size: 1064 138 608 2036 2062 374 146 1280 Fragments arranged by size: 2062 2036 1280 1064 608 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2250 2666 3481 3800 4112 4217 4812 Size: 393 618 416 815 319 312 105 595 Cuts at: 4812 4860 4898 5771 6368 7155 7423 1239 Size: 48 38 873 597 787 268 1524 Fragments arranged by size: 1524 873 815 787 618 597 595 416 393 319 312 268 105 48 38 EcoNI CCTnn'n_nnAGG Cuts at: 2659 2659 Size: 7708 EcoO109I rG'GnC_Cy Cuts at: 3174 4267 4268 4374 4486 4487 4577 4902 3174 Size: 1093 1 106 112 1 90 325 5980 Fragments arranged by size: 5980 1093 325 112 106 90 1 1 EcoRI G'AATT_C Cuts at: 2849 5017 5395 2849 Size: 2168 378 5162 Fragments arranged by size: 5162 2168 378 EcoRV GAT'ATC Cuts at: 3943 3943 Size: 7708 FspI TGC'GCA Cuts at: 6214 6214 Size: 7708 HaeII r_GCGC'y Cuts at: 1678 4611 4756 6855 1678 Size: 2933 145 2099 2531 Fragments arranged by size: 2933 2531 2099 145 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2147 2179 2933 2965 3145 Size: 32 135 32 575 32 754 32 180 Cuts at: 3145 3177 3479 3511 4177 4209 4913 4945 6349 Size: 32 302 32 666 32 704 32 1404 Cuts at: 6349 6381 6423 6455 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1404 754 704 666 575 302 180 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5052 5298 669 Size: 988 281 3114 246 3079 Fragments arranged by size: 3114 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7708 HpaI GTT'AAC Cuts at: 5298 5298 Size: 7708 KasI G'GCGC_C Cuts at: 4607 4752 4607 Size: 145 7563 KpnI G_GTAC'C Cuts at: 5033 5033 Size: 7708 MfeI C'AATT_G Cuts at: 5307 5307 Size: 7708 MluI A'CGCG_T Cuts at: 7584 7584 Size: 7708 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4863 6822 7006 1305 Size: 3558 1959 184 2007 Fragments arranged by size: 3558 2007 1959 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2241 4452 10 Size: 54 1425 343 409 2211 3266 Fragments arranged by size: 3266 2211 1425 409 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2084 2235 2258 2835 3462 4647 4767 Size: 648 918 151 23 577 627 1185 120 Cuts at: 4767 5666 6025 6184 518 Size: 899 359 159 2042 Fragments arranged by size: 2042 1185 918 899 648 627 577 359 159 151 120 23 MspA1I CmG'CkG Cuts at: 1606 2733 4038 4067 5124 5801 6949 7194 1606 Size: 1127 1305 29 1057 677 1148 245 2120 Fragments arranged by size: 2120 1305 1148 1127 1057 677 245 29 NaeI GCC'GGC Cuts at: 4502 4502 Size: 7708 NarI GG'CG_CC Cuts at: 4608 4753 4608 Size: 145 7563 NcoI C'CATG_G Cuts at: 513 1065 2079 2253 2925 3406 3726 513 Size: 552 1014 174 672 481 320 4495 Fragments arranged by size: 4495 1014 672 552 481 320 174 NdeI CA'TA_TG Cuts at: 387 3451 387 Size: 3064 4644 NgoMIV G'CCGG_C Cuts at: 4500 4500 Size: 7708 NheI G'CTAG_C Cuts at: 1052 4280 1052 Size: 3228 4480 NotI GC'GGCC_GC Cuts at: 5155 5155 Size: 7708 NspI r_CATG'y Cuts at: 1132 1229 4770 5066 6611 1132 Size: 97 3541 296 1545 2229 Fragments arranged by size: 3541 2229 1545 296 97 PasI CC'CwG_GG Cuts at: 1348 3686 4275 4603 4615 4634 1348 Size: 2338 589 328 12 19 4422 Fragments arranged by size: 4422 2338 589 328 19 12 PciI A'CATG_T Cuts at: 4766 6607 4766 Size: 1841 5867 PflMI CCAn_nnn'nTGG Cuts at: 1108 2431 3221 3328 1108 Size: 1323 790 107 5488 Fragments arranged by size: 5488 1323 790 107 PfoI T'CCnGG_A Cuts at: 4245 4564 4850 4245 Size: 319 286 7103 Fragments arranged by size: 7103 319 286 PmeI GTTT'AAAC Cuts at: 5012 5012 Size: 7708 PmlI CAC'GTG Cuts at: 2491 2491 Size: 7708 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5777 5809 7290 7322 5777 Size: 32 1481 32 6163 Fragments arranged by size: 6163 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4374 4374 Size: 7708 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7708 PsiI TTA'TAA Cuts at: 5278 5278 Size: 7708 PspOMI G'GGCC_C Cuts at: 4267 4486 4267 Size: 219 7489 PstI C_TGCA'G Cuts at: 830 2520 4305 5060 830 Size: 1690 1785 755 3478 Fragments arranged by size: 3478 1785 1690 755 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7708 PvuII CAG'CTG Cuts at: 2733 4038 4067 2733 Size: 1305 29 6374 Fragments arranged by size: 6374 1305 29 SalI G'TCGA_C Cuts at: 1936 5050 1936 Size: 3114 4594 SbfI CC_TGCA'GG Cuts at: 4305 5060 4305 Size: 755 6953 ScaI AGT'ACT Cuts at: 2366 3905 5956 7668 2366 Size: 1539 2051 1712 2406 Fragments arranged by size: 2406 2051 1712 1539 SfcI C'TryA_G Cuts at: 826 1045 2516 4301 5056 6191 6872 7063 826 Size: 219 1471 1785 755 1135 681 191 1471 Fragments arranged by size: 1785 1471 1471 1135 755 681 219 191 SfoI GGC'GCC Cuts at: 4609 4754 4609 Size: 145 7563 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7708 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7708 SmaI CCC'GGG Cuts at: 1872 4907 5035 1872 Size: 3035 128 4545 Fragments arranged by size: 4545 3035 128 SmlI C'TyrA_G Cuts at: 820 1017 1943 2976 2999 3072 4004 5812 6713 Size: 197 926 1033 23 73 932 1808 901 Cuts at: 6713 6975 820 Size: 262 1553 Fragments arranged by size: 1808 1553 1033 932 926 901 262 197 73 23 SnaBI TAC'GTA Cuts at: 493 5499 493 Size: 5006 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7708 SphI G_CATG'C Cuts at: 1132 5066 1132 Size: 3934 3774 SspI AAT'ATT Cuts at: 5 52 5632 5 Size: 47 5580 2081 Fragments arranged by size: 5580 2081 47 StuI AGG'CCT Cuts at: 1517 3893 1517 Size: 2376 5332 StyI C'CwwG_G Cuts at: 513 1065 2079 2142 2253 2925 2982 3406 3726 Size: 552 1014 63 111 672 57 424 320 Cuts at: 3726 3894 4587 4645 5147 513 Size: 168 693 58 502 3074 Fragments arranged by size: 3074 1014 693 672 552 502 424 320 168 111 63 58 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2600 3562 4423 5735 5752 5905 6090 Size: 1255 1020 962 861 1312 17 153 185 Cuts at: 6090 6535 325 Size: 445 1498 Fragments arranged by size: 1498 1312 1255 1020 962 861 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2364 3903 4143 Size: 274 80 33 51 1443 387 1539 240 Cuts at: 4143 5954 7666 96 Size: 1811 1712 138 Fragments arranged by size: 1811 1712 1539 1443 387 274 240 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2434 2466 2557 2589 7668 7700 2434 Size: 32 91 32 5079 32 2442 Fragments arranged by size: 5079 2442 91 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3998 4372 1316 Size: 2682 374 4652 Fragments arranged by size: 4652 2682 374 XbaI T'CTAG_A Cuts at: 3193 5044 3193 Size: 1851 5857 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2932 3456 2932 Size: 524 7184 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7708 XmaI C'CCGG_G Cuts at: 1870 4905 5033 1870 Size: 3035 128 4545 Fragments arranged by size: 4545 3035 128 XmnI GAAnn'nnTTC Cuts at: 5837 5837 Size: 7708 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5516 276 Size: 53 83 186 936 3982 2468 Fragments arranged by size: 3982 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BfrBI BsiWI BsmBI BstZ17I EcoICRI FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI