Restriction map for
pFN21AE9536
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# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp700I AspI AviII BciVI BfrI
BsiWI Bsp1407I BspLU11I BsrDI BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
CpoI DraIII DrdI EcoO65I EcoRV
EcoT22I FseI FspI HpaI MfeI
MluI MunI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 467 GGTACC G^GTACC
AccI 1312 GTCTAC GT^[AC][GT]AC
AccIII 1119 TCCGGA T^CCGGA
AhdI 3042 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3154 CCTAAGG CC^T[ACGT]AGG
Aor51HI 2641 AGCGCT AGC^GCT
ApaI 724 GGGCCC GGGCC^C
Asp718I 467 GGTACC G^GTACC
AspEI 3042 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 2467 CACGTG CAC^GTG
BclI 2198 TGATCA T^GATCA
BglI 834 GCCCGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3623 AGATCT A^GATCT
BlpI 43 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 43 GCTCAGC GC^T[ACGT]AGC
BsaBI 2072 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1119 TCCGGA [AT]^CCGG[AT]
BseAI 1119 TCCGGA T^CCGGA
BsgI 3323 GTGCAG GTGCAG(16/14)
BsmBI 2265 GAGACG CGTCTC(1/5)
BsmI 1816 GCATTC GAATGC(1/-1)
BspDI 2688 ATCGAT AT^CGAT
BspEI 1119 TCCGGA T^CCGGA
BsrBI 186 CCGCTC CCGCTC(-3/-3)
BssHII 350 GCGCGC G^CGCGC
BstAPI 441 GCAGAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 236 CCATGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3154 CCTAAGG CC^T[ACGT]AGG
CelII 43 GCTCAGC GC^T[ACGT]AGC
ClaI 2688 ATCGAT AT^CGAT
DraI 3637 TTTAAA TTT^AAA
Eam1105I 3042 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2641 AGCGCT AGC^GCT
Eco81I 3154 CCTAAGG CC^T[ACGT]AGG
EcoNI 489 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2198 TGATCA T^GATCA
HgaI 2267 GACGC GACGC(5/10)
HindIII 3256 AAGCTT A^AGCTT
KpnI 467 GGTACC GGTAC^C
KspI 708 CCGCGG CCGC^GG
MamI 2072 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1119 TCCGGA T^CCGGA
NaeI 2297 GCCGGC GCC^GGC
NdeI 2045 CATATG CA^TATG
NgoMIV 2297 GCCGGC G^CCGGC
NspI 1301 GCATGC [GA]CATG^[CT]
PaeR7I 104 CTCGAG C^TCGAG
PmaCI 2467 CACGTG CAC^GTG
PmlI 2467 CACGTG CAC^GTG
PpuMI 112 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1910 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 418 CTGCAG CTGCA^G
SacI 1858 GAGCTC GAGCT^C
SacII 708 CCGCGG CCGC^GG
SexAI 884 ACCTGGT A^CC[AT]GGT
SphI 1301 GCATGC GCATG^C
XcmI 641 CCAACGCCACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 104 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 67 GGCGCC G[GA]^CG[CT]C
2326 GGCGCC
AlwNI 472 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1748 CAGTTGCTG
BamHI 2077 GGATCC G^GATCC
2127 GGATCC
BbeI 67 GGCGCC GGCGC^C
2326 GGCGCC
BbvCI 2652 CCTCAGC CCTCAGC(-5/-2)
3163 GCTGAGG
BcgI 286 CGAGCCAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
141 GCAGCCGGGTCG
BsaAI 2467 CACGTG [CT]AC^GT[GA]
3082 CACGTA
BsaHI 67 GGCGCC G[GA]^CG[CT]C
2326 GGCGCC
BsiEI 91 CGGCCG CG[GA][CT]^CG
300 CGGCCG
BspMI 3570 ACCTGC ACCTGC(4/8)
2767 GCAGGT
BsrFI 555 ACCGGC [GA]^CCGG[CT]
2297 GCCGGC
Cfr10I 555 ACCGGC [GA]^CCGG[CT]
2297 GCCGGC
EagI 91 CGGCCG C^GGCCG
300 CGGCCG
EclXI 91 CGGCCG C^GGCCG
300 CGGCCG
Eco52I 91 CGGCCG C^GGCCG
300 CGGCCG
EcoRI 895 GAATTC G^AATTC
1550 GAATTC
Hin1I 67 GGCGCC G[GA]^CG[CT]C
2326 GGCGCC
HincII 916 GTTGAC GT[CT]^[GA]AC
2941 GTCAAC
HindII 916 GTTGAC GT[CT]^[GA]AC
2941 GTCAAC
KasI 67 GGCGCC G^GCGCC
2326 GGCGCC
NarI 67 GGCGCC GG^CGCC
2326 GGCGCC
SfoI 67 GGCGCC GGC^GCC
2326 GGCGCC
StuI 1023 AGGCCT AGG^CCT
1272 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
AvrII BalI BanI BanII BbsI
BbvI BcnI BfaI BlnI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI