(Circular) (Six-base) MAPSORT of: pFN21AE9536.seq Check: 3152 from: 1 to: 8350 pFN21AE9536 8350 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4763 4763 Size: 8350 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6160 278 Size: 53 83 186 936 4624 2468 Fragments arranged by size: 4624 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3318 5693 1937 Size: 1381 2375 4594 Fragments arranged by size: 4594 2375 1381 Acc65I G'GTAC_C Cuts at: 2472 5671 2472 Size: 3199 5151 AclI AA'CG_TT Cuts at: 1563 6477 6850 1563 Size: 4914 373 3063 Fragments arranged by size: 4914 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2306 2521 2918 3902 4374 5049 5232 6413 Size: 674 215 397 984 472 675 183 1181 Cuts at: 6413 7797 8065 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1181 984 675 674 472 397 268 215 183 AfeI AGC'GCT Cuts at: 4648 4648 Size: 8350 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8153 AflIII A'CryG_T Cuts at: 1203 7249 8226 1203 Size: 6046 977 1327 Fragments arranged by size: 6046 1327 977 AgeI A'CCGG_T Cuts at: 7181 7181 Size: 8350 AhdI GACnn_n'nnGTC Cuts at: 5052 7079 5052 Size: 2027 6323 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8350 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8350 AlwNI CAG_nnn'CTG Cuts at: 2482 3758 6093 7665 2482 Size: 1276 2335 1572 3167 Fragments arranged by size: 3167 2335 1572 1276 ApaI G_GGCC'C Cuts at: 2733 2733 Size: 8350 ApaLI G'TGCA_C Cuts at: 6407 7563 6407 Size: 1156 7194 ApoI r'AATT_y Cuts at: 1493 2900 3555 3655 3761 4540 4843 5659 5885 Size: 1407 655 100 106 779 303 816 226 Cuts at: 5885 6037 8146 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1407 816 779 655 303 226 152 106 100 AseI AT'TA_AT Cuts at: 160 1032 6904 160 Size: 872 5872 1606 Fragments arranged by size: 5872 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8350 AvaI C'yCGr_G Cuts at: 1870 1943 2109 2520 2733 2840 5675 1870 Size: 73 166 411 213 107 2835 4545 Fragments arranged by size: 4545 2835 411 213 166 107 73 AvrII C'CTAG_G Cuts at: 3031 4252 5615 3031 Size: 1221 1363 5766 Fragments arranged by size: 5766 1363 1221 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4084 4117 4084 Size: 33 8317 BamHI G'GATC_C Cuts at: 4082 4132 5680 6047 4082 Size: 50 1548 367 6385 Fragments arranged by size: 6385 1548 367 50 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2072 2204 2236 2472 4331 Size: 325 217 627 285 132 32 236 1859 Cuts at: 4331 4621 4680 4982 5671 7126 618 Size: 290 59 302 689 1455 1842 Fragments arranged by size: 1859 1842 1455 689 627 325 302 290 285 236 217 132 59 32 BanII G_rGCy'C Cuts at: 1391 2511 2733 2772 3466 3834 3867 5669 1391 Size: 1120 222 39 694 368 33 1802 4072 Fragments arranged by size: 4072 1802 1120 694 368 222 39 33 BbeI G_GCGC'C Cuts at: 2076 4335 2076 Size: 2259 6091 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2566 3461 4380 4429 4599 4745 928 Size: 971 667 895 919 49 170 146 4533 Fragments arranged by size: 4533 971 919 895 667 170 146 49 BbvCI CC'TCA_GC Cuts at: 4658 5169 4658 Size: 511 7839 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2135 2169 2280 2314 6541 6575 1903 Size: 34 198 34 111 34 4227 34 3678 Fragments arranged by size: 4227 3678 198 111 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6242 7452 6242 Size: 1210 7140 BclI T'GATC_A Cuts at: 1548 4203 1548 Size: 2655 5695 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2845 6961 136 Size: 107 122 71 631 605 1173 4116 1525 Fragments arranged by size: 4116 1525 1173 631 605 122 107 71 BglII A'GATC_T Cuts at: 5628 8345 5628 Size: 2717 5633 BlpI GC'TnA_GC Cuts at: 2049 5767 2049 Size: 3718 4632 Bme1580I G_kGCm'C Cuts at: 1790 2241 2733 4683 6411 7567 1790 Size: 451 492 1950 1728 1156 2573 Fragments arranged by size: 2573 1950 1728 1156 492 451 BmgBI CAC'GTC Cuts at: 1318 2248 1318 Size: 930 7420 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4686 6081 7039 Size: 416 53 83 399 24 3269 1395 958 Cuts at: 7039 442 Size: 1753 Fragments arranged by size: 3269 1753 1395 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8350 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2206 2519 2564 2678 2834 3108 4135 4208 Size: 967 313 45 114 156 274 1027 73 Cuts at: 4208 5265 7010 1239 Size: 1057 1745 2579 Fragments arranged by size: 2579 1745 1057 1027 967 313 274 156 114 73 45 Bpu10I CC'TnA_GC Cuts at: 4658 5169 4658 Size: 511 7839 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2944 3895 4430 5458 6475 7340 7638 2944 Size: 951 535 1028 1017 865 298 3656 Fragments arranged by size: 3656 1028 1017 951 865 535 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2654 2805 4412 4473 5528 7013 882 Size: 617 1155 151 1607 61 1055 1485 2219 Fragments arranged by size: 2219 1607 1485 1155 1055 617 151 61 BsaAI yAC'GTr Cuts at: 493 1204 4474 5089 6141 493 Size: 711 3270 615 1052 2702 Fragments arranged by size: 3270 2702 1052 711 615 BsaBI GATnn'nnATC Cuts at: 4081 8344 4081 Size: 4263 4087 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2073 4332 6157 6539 Size: 53 83 186 936 540 2259 1825 382 Cuts at: 6539 7174 275 Size: 635 1451 Fragments arranged by size: 2259 1825 1451 936 635 540 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3124 6783 7181 7455 7602 8013 3124 Size: 3659 398 274 147 411 3461 Fragments arranged by size: 3659 3461 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2609 2639 2651 2681 3106 Size: 30 165 30 1206 30 12 30 425 Cuts at: 3106 3136 1178 Size: 30 6392 Fragments arranged by size: 6392 1206 425 165 30 30 30 30 30 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2021 2164 2246 2609 2617 4430 4538 4541 Size: 835 143 82 363 8 1813 108 3 Cuts at: 4541 4913 5321 5462 1186 Size: 372 408 141 4074 Fragments arranged by size: 4074 1813 835 408 372 363 143 141 108 82 8 3 BseYI C'CCAG_C Cuts at: 2053 2142 2510 3735 4754 5345 5539 7553 8049 Size: 89 368 1225 1019 591 194 2014 496 Cuts at: 8049 2053 Size: 2354 Fragments arranged by size: 2354 2014 1225 1019 591 496 368 194 89 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5349 1163 Size: 573 3613 4164 Fragments arranged by size: 4164 3613 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2099 2308 5800 6561 7589 1540 Size: 275 186 98 209 3492 761 1028 2301 Fragments arranged by size: 3492 2301 1028 761 275 209 186 98 BsiHKAI G_wGCw'C Cuts at: 3867 4125 4241 4313 6411 6496 7567 3867 Size: 258 116 72 2098 85 1071 4650 Fragments arranged by size: 4650 2098 1071 258 116 85 72 BsmI GAATG_Cn' Cuts at: 3821 5861 5954 3821 Size: 2040 93 6217 Fragments arranged by size: 6217 2040 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4264 4264 Size: 8350 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2241 2511 2733 2772 3466 3834 3867 Size: 399 451 270 222 39 694 368 33 Cuts at: 3867 4125 4241 4313 4683 5669 6411 6496 7567 Size: 258 116 72 370 986 742 85 1071 Cuts at: 7567 1391 Size: 2174 Fragments arranged by size: 2174 1071 986 742 694 451 399 370 368 270 258 222 116 85 72 39 33 BspEI T'CCGG_A Cuts at: 3124 8013 3124 Size: 4889 3461 BspHI T'CATG_A Cuts at: 2913 3582 4266 6239 7969 2913 Size: 669 684 1973 1730 3294 Fragments arranged by size: 3294 1973 1730 684 669 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4763 5584 5705 844 Size: 3919 821 121 3489 Fragments arranged by size: 3919 3489 821 121 BsrBI CCG'CTC Cuts at: 1394 1823 2193 6237 1394 Size: 429 370 4044 3507 Fragments arranged by size: 4044 3507 429 370 BsrDI GCAATG_nn' Cuts at: 65 1248 6845 7019 65 Size: 1183 5597 174 1396 Fragments arranged by size: 5597 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2560 4302 6994 7181 1700 Size: 110 750 1742 2692 187 2869 Fragments arranged by size: 2869 2692 1742 750 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8350 BssHII G'CGCG_C Cuts at: 1925 2355 1925 Size: 430 7920 BssSI C'ACGA_G Cuts at: 4026 5325 5505 6410 7422 4026 Size: 1299 180 905 1012 4954 Fragments arranged by size: 4954 1299 1012 905 180 BstAPI GCAn_nnn'nTGC Cuts at: 794 2452 794 Size: 1658 6692 BstBI TT'CG_AA Cuts at: 7154 7154 Size: 8350 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8350 BstXI CCAn_nnnn'nTGG Cuts at: 2248 2248 Size: 8350 BstYI r'GATC_y Cuts at: 1972 3386 4082 4132 4284 4867 5628 5680 6047 Size: 1414 696 50 152 583 761 52 367 Cuts at: 6047 6432 6449 7901 7987 7999 8345 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1414 761 696 583 385 367 346 152 86 52 50 17 12 Bsu36I CC'TnA_GG Cuts at: 5160 5160 Size: 8350 BtgI C'CryG_G Cuts at: 513 1065 2004 2013 2241 2674 2713 4339 4611 Size: 552 939 9 228 433 39 1626 272 Cuts at: 4611 513 Size: 4252 Fragments arranged by size: 4252 1626 939 552 433 272 228 39 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7611 Fragments arranged by size: 7611 683 56 BtsI GCAGTG_nn' Cuts at: 3807 4313 4356 4956 5866 6660 6680 3807 Size: 506 43 600 910 794 20 5477 Fragments arranged by size: 5477 910 794 600 506 43 20 ClaI AT'CG_AT Cuts at: 1554 4694 1554 Size: 3140 5210 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8315 DraI TTT'AAA Cuts at: 5644 5654 5644 Size: 10 8340 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7357 809 Size: 507 6041 1802 Fragments arranged by size: 6041 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2096 2305 2339 Size: 54 1425 50 275 18 266 209 34 Cuts at: 2339 2349 2526 2643 3682 5797 6686 8315 8 Size: 10 177 117 1039 2115 889 1629 43 Fragments arranged by size: 2115 1629 1425 1039 889 275 266 209 177 117 54 50 43 34 18 10 EagI C'GGCC_G Cuts at: 1537 1812 2096 2305 5797 1537 Size: 275 284 209 3492 4090 Fragments arranged by size: 4090 3492 284 275 209 EarI CTCTTCn'nnn_ Cuts at: 1353 4485 5587 5619 6280 1353 Size: 3132 1102 32 661 3423 Fragments arranged by size: 3423 3132 1102 661 32 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2793 3588 6937 7311 7457 387 Size: 1064 138 1204 795 3349 374 146 1280 Fragments arranged by size: 3349 1280 1204 1064 795 374 146 138 EcoICRI GAG'CTC Cuts at: 3865 3865 Size: 8350 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2206 2306 2519 2521 2564 2678 2834 Size: 393 574 100 213 2 43 114 156 Cuts at: 2834 2918 3108 3902 4135 4208 4374 5049 5232 Size: 84 190 794 233 73 166 675 183 Cuts at: 5232 5265 6413 7010 7797 8065 1239 Size: 33 1148 597 787 268 1524 Fragments arranged by size: 1524 1148 794 787 675 597 574 393 268 233 213 190 183 166 156 114 100 84 73 43 33 2 EcoNI CCTnn'n_nnAGG Cuts at: 2498 2498 Size: 8350 EcoO109I rG'GnC_Cy Cuts at: 2118 2697 2729 2730 2737 4249 5612 2118 Size: 579 32 1 7 1512 1363 4856 Fragments arranged by size: 4856 1512 1363 579 32 7 1 EcoRI G'AATT_C Cuts at: 2900 3555 5659 6037 2900 Size: 655 2104 378 5213 Fragments arranged by size: 5213 2104 655 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2806 5020 5237 5586 2806 Size: 2214 217 349 5570 Fragments arranged by size: 5570 2214 349 217 FspI TGC'GCA Cuts at: 6856 6856 Size: 8350 HaeII r_GCGC'y Cuts at: 1678 2076 2381 2483 4335 4650 4731 7497 1678 Size: 398 305 102 1852 315 81 2766 2531 Fragments arranged by size: 2766 2531 1852 398 315 305 102 81 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2237 2269 2537 2569 2609 Size: 32 135 32 665 32 268 32 40 Cuts at: 2609 2641 2942 2974 3106 3138 3246 3278 3378 Size: 32 301 32 132 32 108 32 100 Cuts at: 3378 3410 4592 4624 4852 4884 5038 5070 5527 Size: 32 1182 32 228 32 154 32 457 Cuts at: 5527 5559 6991 7023 7065 7097 1373 Size: 32 1432 32 42 32 2626 Fragments arranged by size: 2626 1432 1182 665 457 301 268 228 154 135 132 108 100 42 40 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2923 4948 5694 5940 669 Size: 988 281 985 2025 746 246 3079 Fragments arranged by size: 3079 2025 988 985 746 281 246 HindIII A'AGCT_T Cuts at: 748 5261 748 Size: 4513 3837 HpaI GTT'AAC Cuts at: 5940 5940 Size: 8350 KasI G'GCGC_C Cuts at: 2072 4331 2072 Size: 2259 6091 KpnI G_GTAC'C Cuts at: 2476 5675 2476 Size: 3199 5151 MfeI C'AATT_G Cuts at: 5949 5949 Size: 8350 MluI A'CGCG_T Cuts at: 8226 8226 Size: 8350 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3348 7464 7648 1305 Size: 2043 4116 184 2007 Fragments arranged by size: 4116 2043 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2341 2351 2645 3684 10 Size: 54 1425 343 509 10 294 1039 4676 Fragments arranged by size: 4676 1425 1039 509 343 294 54 10 MslI CAynn'nnrTG Cuts at: 518 1166 2246 3311 3587 3646 4991 6308 6667 Size: 648 1080 1065 276 59 1345 1317 359 Cuts at: 6667 6826 518 Size: 159 2042 Fragments arranged by size: 2042 1345 1317 1080 1065 648 359 276 159 59 MspA1I CmG'CkG Cuts at: 1606 2053 2284 2715 2751 3735 5168 5766 6443 Size: 447 231 431 36 984 1433 598 677 Cuts at: 6443 7591 7836 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1433 1148 984 677 598 447 431 245 231 36 NaeI GCC'GGC Cuts at: 4304 4304 Size: 8350 NarI GG'CG_CC Cuts at: 2073 4332 2073 Size: 2259 6091 NcoI C'CATG_G Cuts at: 513 1065 2004 2013 2241 4339 513 Size: 552 939 9 228 2098 4524 Fragments arranged by size: 4524 2098 939 552 228 9 NdeI CA'TA_TG Cuts at: 387 4051 387 Size: 3664 4686 NgoMIV G'CCGG_C Cuts at: 4302 4302 Size: 8350 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8350 NotI GC'GGCC_GC Cuts at: 5797 5797 Size: 8350 NspI r_CATG'y Cuts at: 1132 1229 3310 5708 7253 1132 Size: 97 2081 2398 1545 2229 Fragments arranged by size: 2398 2229 2081 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8350 PciI A'CATG_T Cuts at: 7249 7249 Size: 8350 PflMI CCAn_nnn'nTGG Cuts at: 1108 2482 3658 5555 1108 Size: 1374 1176 1897 3903 Fragments arranged by size: 3903 1897 1374 1176 PfoI T'CCnGG_A Cuts at: 2895 3085 3234 2895 Size: 190 149 8011 Fragments arranged by size: 8011 190 149 PmeI GTTT'AAAC Cuts at: 5654 5654 Size: 8350 PmlI CAC'GTG Cuts at: 4474 4474 Size: 8350 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3487 3519 6419 6451 7932 7964 3487 Size: 32 2900 32 1481 32 3873 Fragments arranged by size: 3873 2900 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2118 2118 Size: 8350 PshAI GACnn'nnGTC Cuts at: 1613 3919 1613 Size: 2306 6044 PsiI TTA'TAA Cuts at: 5920 5920 Size: 8350 PspOMI G'GGCC_C Cuts at: 2729 2729 Size: 8350 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2816 2848 2816 Size: 32 8318 PstI C_TGCA'G Cuts at: 830 2427 5702 830 Size: 1597 3275 3478 Fragments arranged by size: 3478 3275 1597 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8350 PvuII CAG'CTG Cuts at: 2053 2284 2751 5168 2053 Size: 231 467 2417 5235 Fragments arranged by size: 5235 2417 467 231 SacI G_AGCT'C Cuts at: 3867 3867 Size: 8350 SacII CC_GC'GG Cuts at: 2716 2716 Size: 8350 SalI G'TCGA_C Cuts at: 1936 5692 1936 Size: 3756 4594 SbfI CC_TGCA'GG Cuts at: 5702 5702 Size: 8350 ScaI AGT'ACT Cuts at: 6598 8310 6598 Size: 1712 6638 SexAI A'CCwGG_T Cuts at: 2889 2889 Size: 8350 SfcI C'TryA_G Cuts at: 826 1045 2423 4041 5376 5698 6833 7514 7705 Size: 219 1378 1618 1335 322 1135 681 191 Cuts at: 7705 826 Size: 1471 Fragments arranged by size: 1618 1471 1378 1335 1135 681 322 219 191 SfoI GGC'GCC Cuts at: 2074 4333 2074 Size: 2259 6091 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8350 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8350 SmaI CCC'GGG Cuts at: 1872 2522 2735 2842 5677 1872 Size: 650 213 107 2835 4545 Fragments arranged by size: 4545 2835 650 213 107 SmlI C'TyrA_G Cuts at: 820 1017 1943 2109 2959 3910 4445 5473 6454 Size: 197 926 166 850 951 535 1028 981 Cuts at: 6454 7355 7617 820 Size: 901 262 1553 Fragments arranged by size: 1553 1028 981 951 926 901 850 535 262 197 166 SnaBI TAC'GTA Cuts at: 493 6141 493 Size: 5648 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8350 SphI G_CATG'C Cuts at: 1132 3310 5708 1132 Size: 2178 2398 3774 Fragments arranged by size: 3774 2398 2178 SspI AAT'ATT Cuts at: 5 52 6274 5 Size: 47 6222 2081 Fragments arranged by size: 6222 2081 47 StuI AGG'CCT Cuts at: 1517 3030 3279 1517 Size: 1513 249 6588 Fragments arranged by size: 6588 1513 249 StyI C'CwwG_G Cuts at: 513 1065 2004 2013 2089 2241 3031 3274 4252 Size: 552 939 9 76 152 790 243 978 Cuts at: 4252 4339 4410 4731 5027 5233 5522 5615 5789 Size: 87 71 321 296 206 289 93 174 Cuts at: 5789 513 Size: 3074 Fragments arranged by size: 3074 978 939 790 552 321 296 289 243 206 174 152 93 87 76 71 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2678 4698 5003 6377 6394 6547 6732 Size: 1255 1098 2020 305 1374 17 153 185 Cuts at: 6732 7177 325 Size: 445 1498 Fragments arranged by size: 2020 1498 1374 1255 1098 445 305 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3146 3420 6596 Size: 274 80 33 51 1443 1169 274 3176 Cuts at: 6596 8308 96 Size: 1712 138 Fragments arranged by size: 3176 1712 1443 1169 274 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2559 2591 2651 2683 8310 8342 2559 Size: 32 60 32 5627 32 2567 Fragments arranged by size: 5627 2567 60 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8350 XbaI T'CTAG_A Cuts at: 5686 5686 Size: 8350 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2653 2011 Size: 642 7708 XhoI C'TCGA_G Cuts at: 1943 2109 1943 Size: 166 8184 XmaI C'CCGG_G Cuts at: 1870 2520 2733 2840 5675 1870 Size: 650 213 107 2835 4545 Fragments arranged by size: 4545 2835 650 213 107 XmnI GAAnn'nnTTC Cuts at: 6479 6479 Size: 8350 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6158 276 Size: 53 83 186 936 4624 2468 Fragments arranged by size: 4624 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PspXI RsrII SanDI SapI SfiI SrfI SwaI