(Linear) (Six-base) MAPSORT of: KIEE9536.seq Check: 8755 from: 1 to: 3645 KIEE9536.orf With 182 enzymes: SgfI * July 3, 2012 19:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2758 3645 Size: 2758 887 AccI GT'mk_AC Cuts at: 0 1313 3645 Size: 1313 2332 Acc65I G'GTAC_C Cuts at: 0 467 3645 Size: 467 3178 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 301 516 913 1897 2369 3044 3227 3645 Size: 301 215 397 984 472 675 183 418 Fragments arranged by size: 984 675 472 418 397 301 215 183 AfeI AGC'GCT Cuts at: 0 2643 3645 Size: 2643 1002 AhdI GACnn_n'nnGTC Cuts at: 0 3047 3645 Size: 3047 598 AlwNI CAG_nnn'CTG Cuts at: 0 477 1753 3645 Size: 477 1276 1892 Fragments arranged by size: 1892 1276 477 ApaI G_GGCC'C Cuts at: 0 728 3645 Size: 728 2917 ApoI r'AATT_y Cuts at: 0 895 1550 1650 1756 2535 2838 3645 Size: 895 655 100 106 779 303 807 Fragments arranged by size: 895 807 779 655 303 106 100 AvaI C'yCGr_G Cuts at: 0 104 515 728 835 3645 Size: 104 411 213 107 2810 Fragments arranged by size: 2810 411 213 107 104 AvrII C'CTAG_G Cuts at: 0 1026 2247 3610 3645 Size: 1026 1221 1363 35 Fragments arranged by size: 1363 1221 1026 35 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2079 2112 3645 Size: 2079 33 1533 Fragments arranged by size: 2079 1533 33 BamHI G'GATC_C Cuts at: 0 2077 2127 3645 Size: 2077 50 1518 Fragments arranged by size: 2077 1518 50 BanI G'GyrC_C Cuts at: 0 67 199 231 467 2326 2616 2675 2977 Size: 67 132 32 236 1859 290 59 302 Cuts at: 2977 3645 Size: 668 Fragments arranged by size: 1859 668 302 290 236 132 67 59 32 BanII G_rGCy'C Cuts at: 0 506 728 767 1461 1829 1862 3645 Size: 506 222 39 694 368 33 1783 Fragments arranged by size: 1783 694 506 368 222 39 33 BbeI G_GCGC'C Cuts at: 0 71 2330 3645 Size: 71 2259 1315 Fragments arranged by size: 2259 1315 71 BbsI GAAGACnn'nnnn_ Cuts at: 0 561 1456 2375 2424 2594 2740 3645 Size: 561 895 919 49 170 146 905 Fragments arranged by size: 919 905 895 561 170 146 49 BbvCI CC'TCA_GC Cuts at: 0 2653 3164 3645 Size: 2653 511 481 Fragments arranged by size: 2653 511 481 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 130 164 275 309 3645 Size: 130 34 111 34 3336 Fragments arranged by size: 3336 130 111 34 34 BclI T'GATC_A Cuts at: 0 2198 3645 Size: 2198 1447 BglI GCCn_nnn'nGGC Cuts at: 0 840 3645 Size: 840 2805 BglII A'GATC_T Cuts at: 0 3623 3645 Size: 3623 22 BlpI GC'TnA_GC Cuts at: 0 44 3645 Size: 44 3601 Bme1580I G_kGCm'C Cuts at: 0 236 728 2678 3645 Size: 236 492 1950 967 Fragments arranged by size: 1950 967 492 236 BmgBI CAC'GTC Cuts at: 0 243 3645 Size: 243 3402 BmrI ACTGGGnnnn_n' Cuts at: 0 2681 3645 Size: 2681 964 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 201 514 559 673 829 1103 2130 2203 Size: 201 313 45 114 156 274 1027 73 Cuts at: 2203 3260 3645 Size: 1057 385 Fragments arranged by size: 1057 1027 385 313 274 201 156 114 73 45 Bpu10I CC'TnA_GC Cuts at: 0 2653 3164 3645 Size: 2653 511 481 Fragments arranged by size: 2653 511 481 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 939 1890 2425 3453 3645 Size: 939 951 535 1028 192 Fragments arranged by size: 1028 951 939 535 192 BsaI GGTCTCn'nnnn_ Cuts at: 0 649 800 2407 2468 3523 3645 Size: 649 151 1607 61 1055 122 Fragments arranged by size: 1607 1055 649 151 122 61 BsaAI yAC'GTr Cuts at: 0 2469 3084 3645 Size: 2469 615 561 Fragments arranged by size: 2469 615 561 BsaBI GATnn'nnATC Cuts at: 0 2076 3645 Size: 2076 1569 BsaHI Gr'CG_yC Cuts at: 0 68 2327 3645 Size: 68 2259 1318 Fragments arranged by size: 2259 1318 68 BsaWI w'CCGG_w Cuts at: 0 1119 3645 Size: 1119 2526 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 604 634 646 676 1101 1131 3645 Size: 604 30 12 30 425 30 2514 Fragments arranged by size: 2514 604 425 30 30 30 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 16 159 241 604 612 2425 2533 2536 Size: 16 143 82 363 8 1813 108 3 Cuts at: 2536 2908 3316 3457 3645 Size: 372 408 141 188 Fragments arranged by size: 1813 408 372 363 188 143 141 108 82 16 8 3 BseYI C'CCAG_C Cuts at: 0 48 137 505 1730 2749 3340 3534 3645 Size: 48 89 368 1225 1019 591 194 111 Fragments arranged by size: 1225 1019 591 368 194 111 89 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3344 3645 Size: 3344 301 BsiEI CG_ry'CG Cuts at: 0 94 303 3645 Size: 94 209 3342 Fragments arranged by size: 3342 209 94 BsiHKAI G_wGCw'C Cuts at: 0 1862 2120 2236 2308 3645 Size: 1862 258 116 72 1337 Fragments arranged by size: 1862 1337 258 116 72 BsmI GAATG_Cn' Cuts at: 0 1816 3645 Size: 1816 1829 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2259 3645 Size: 2259 1386 Bsp1286I G_dGCh'C Cuts at: 0 236 506 728 767 1461 1829 1862 2120 Size: 236 270 222 39 694 368 33 258 Cuts at: 2120 2236 2308 2678 3645 Size: 116 72 370 967 Fragments arranged by size: 967 694 370 368 270 258 236 222 116 72 39 33 BspEI T'CCGG_A Cuts at: 0 1119 3645 Size: 1119 2526 BspHI T'CATG_A Cuts at: 0 908 1577 2261 3645 Size: 908 669 684 1384 Fragments arranged by size: 1384 908 684 669 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2758 3579 3645 Size: 2758 821 66 Fragments arranged by size: 2758 821 66 BsrBI CCG'CTC Cuts at: 0 188 3645 Size: 188 3457 BsrFI r'CCGG_y Cuts at: 0 555 2297 3645 Size: 555 1742 1348 Fragments arranged by size: 1742 1348 555 BssHII G'CGCG_C Cuts at: 0 350 3645 Size: 350 3295 BssSI C'ACGA_G Cuts at: 0 2021 3320 3500 3645 Size: 2021 1299 180 145 Fragments arranged by size: 2021 1299 180 145 BstAPI GCAn_nnn'nTGC Cuts at: 0 447 3645 Size: 447 3198 BstXI CCAn_nnnn'nTGG Cuts at: 0 243 3645 Size: 243 3402 BstYI r'GATC_y Cuts at: 0 1381 2077 2127 2279 2862 3623 3645 Size: 1381 696 50 152 583 761 22 Fragments arranged by size: 1381 761 696 583 152 50 22 Bsu36I CC'TnA_GG Cuts at: 0 3155 3645 Size: 3155 490 BtgI C'CryG_G Cuts at: 0 8 236 669 708 2334 2606 3645 Size: 8 228 433 39 1626 272 1039 Fragments arranged by size: 1626 1039 433 272 228 39 8 BtsI GCAGTG_nn' Cuts at: 0 1802 2308 2351 2951 3645 Size: 1802 506 43 600 694 Fragments arranged by size: 1802 694 600 506 43 ClaI AT'CG_AT Cuts at: 0 2689 3645 Size: 2689 956 DraI TTT'AAA Cuts at: 0 3639 3645 Size: 3639 6 EaeI y'GGCC_r Cuts at: 0 91 300 334 344 521 638 1677 3645 Size: 91 209 34 10 177 117 1039 1968 Fragments arranged by size: 1968 1039 209 177 117 91 34 10 EagI C'GGCC_G Cuts at: 0 91 300 3645 Size: 91 209 3345 Fragments arranged by size: 3345 209 91 EarI CTCTTCn'nnn_ Cuts at: 0 2480 3582 3614 3645 Size: 2480 1102 32 31 Fragments arranged by size: 2480 1102 32 31 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 788 1583 3645 Size: 788 795 2062 Fragments arranged by size: 2062 795 788 EcoICRI GAG'CTC Cuts at: 0 1860 3645 Size: 1860 1785 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 201 301 514 516 559 673 829 913 Size: 201 100 213 2 43 114 156 84 Cuts at: 913 1103 1897 2130 2203 2369 3044 3227 3260 Size: 190 794 233 73 166 675 183 33 Cuts at: 3260 3645 Size: 385 Fragments arranged by size: 794 675 385 233 213 201 190 183 166 156 114 100 84 73 43 33 2 EcoNI CCTnn'n_nnAGG Cuts at: 0 493 3645 Size: 493 3152 EcoO109I rG'GnC_Cy Cuts at: 0 113 692 724 725 732 2244 3607 3645 Size: 113 579 32 1 7 1512 1363 38 Fragments arranged by size: 1512 1363 579 113 38 32 7 1 EcoRI G'AATT_C Cuts at: 0 895 1550 3645 Size: 895 655 2095 Fragments arranged by size: 2095 895 655 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 801 3015 3232 3581 3645 Size: 801 2214 217 349 64 Fragments arranged by size: 2214 801 349 217 64 HaeII r_GCGC'y Cuts at: 0 71 376 478 2330 2645 2726 3645 Size: 71 305 102 1852 315 81 919 Fragments arranged by size: 1852 919 315 305 102 81 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 232 264 532 564 604 636 937 969 Size: 232 32 268 32 40 32 301 32 Cuts at: 969 1101 1133 1241 1273 1373 1405 2587 2619 Size: 132 32 108 32 100 32 1182 32 Cuts at: 2619 2847 2879 3033 3065 3522 3554 3645 Size: 228 32 154 32 457 32 91 Fragments arranged by size: 1182 457 301 268 232 228 154 132 108 100 91 40 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 918 2943 3645 Size: 918 2025 702 Fragments arranged by size: 2025 918 702 HindIII A'AGCT_T Cuts at: 0 3256 3645 Size: 3256 389 KasI G'GCGC_C Cuts at: 0 67 2326 3645 Size: 67 2259 1319 Fragments arranged by size: 2259 1319 67 KpnI G_GTAC'C Cuts at: 0 471 3645 Size: 471 3174 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1343 3645 Size: 1343 2302 MscI TGG'CCA Cuts at: 0 336 346 640 1679 3645 Size: 336 10 294 1039 1966 Fragments arranged by size: 1966 1039 336 294 10 MslI CAynn'nnrTG Cuts at: 0 241 1306 1582 1641 2986 3645 Size: 241 1065 276 59 1345 659 Fragments arranged by size: 1345 1065 659 276 241 59 MspA1I CmG'CkG Cuts at: 0 48 279 710 746 1730 3163 3645 Size: 48 231 431 36 984 1433 482 Fragments arranged by size: 1433 984 482 431 231 48 36 NaeI GCC'GGC Cuts at: 0 2299 3645 Size: 2299 1346 NarI GG'CG_CC Cuts at: 0 68 2327 3645 Size: 68 2259 1318 Fragments arranged by size: 2259 1318 68 NcoI C'CATG_G Cuts at: 0 8 236 2334 3645 Size: 8 228 2098 1311 Fragments arranged by size: 2098 1311 228 8 NdeI CA'TA_TG Cuts at: 0 2046 3645 Size: 2046 1599 NgoMIV G'CCGG_C Cuts at: 0 2297 3645 Size: 2297 1348 NspI r_CATG'y Cuts at: 0 1305 3645 Size: 1305 2340 PflMI CCAn_nnn'nTGG Cuts at: 0 477 1653 3550 3645 Size: 477 1176 1897 95 Fragments arranged by size: 1897 1176 477 95 PfoI T'CCnGG_A Cuts at: 0 890 1080 1229 3645 Size: 890 190 149 2416 Fragments arranged by size: 2416 890 190 149 PmlI CAC'GTG Cuts at: 0 2469 3645 Size: 2469 1176 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1482 1514 3645 Size: 1482 32 2131 Fragments arranged by size: 2131 1482 32 PpuMI rG'GwC_Cy Cuts at: 0 113 3645 Size: 113 3532 PshAI GACnn'nnGTC Cuts at: 0 1914 3645 Size: 1914 1731 PspOMI G'GGCC_C Cuts at: 0 724 3645 Size: 724 2921 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 811 843 3645 Size: 811 32 2802 Fragments arranged by size: 2802 811 32 PstI C_TGCA'G Cuts at: 0 422 3645 Size: 422 3223 PvuII CAG'CTG Cuts at: 0 48 279 746 3163 3645 Size: 48 231 467 2417 482 Fragments arranged by size: 2417 482 467 231 48 SacI G_AGCT'C Cuts at: 0 1862 3645 Size: 1862 1783 SacII CC_GC'GG Cuts at: 0 711 3645 Size: 711 2934 SexAI A'CCwGG_T Cuts at: 0 884 3645 Size: 884 2761 SfcI C'TryA_G Cuts at: 0 418 2036 3371 3645 Size: 418 1618 1335 274 Fragments arranged by size: 1618 1335 418 274 SfoI GGC'GCC Cuts at: 0 69 2328 3645 Size: 69 2259 1317 Fragments arranged by size: 2259 1317 69 SmaI CCC'GGG Cuts at: 0 517 730 837 3645 Size: 517 213 107 2808 Fragments arranged by size: 2808 517 213 107 SmlI C'TyrA_G Cuts at: 0 104 954 1905 2440 3468 3645 Size: 104 850 951 535 1028 177 Fragments arranged by size: 1028 951 850 535 177 104 SphI G_CATG'C Cuts at: 0 1305 3645 Size: 1305 2340 StuI AGG'CCT Cuts at: 0 1025 1274 3645 Size: 1025 249 2371 Fragments arranged by size: 2371 1025 249 StyI C'CwwG_G Cuts at: 0 8 84 236 1026 1269 2247 2334 2405 Size: 8 76 152 790 243 978 87 71 Cuts at: 2405 2726 3022 3228 3517 3610 3645 Size: 321 296 206 289 93 35 Fragments arranged by size: 978 790 321 296 289 243 206 152 93 87 76 71 35 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 673 2693 2998 3645 Size: 673 2020 305 647 Fragments arranged by size: 2020 673 647 305 TatI w'GTAC_w Cuts at: 0 1141 1415 3645 Size: 1141 274 2230 Fragments arranged by size: 2230 1141 274 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 554 586 646 678 3645 Size: 554 32 60 32 2967 Fragments arranged by size: 2967 554 60 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 648 3645 Size: 648 2997 XhoI C'TCGA_G Cuts at: 0 104 3645 Size: 104 3541 XmaI C'CCGG_G Cuts at: 0 515 728 835 3645 Size: 515 213 107 2810 Fragments arranged by size: 2810 515 213 107 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AhdI AlwNI ApaI ApoI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI ClaI DraI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PsrI PstI PvuII SacI SacII SexAI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI XcmI XhoI XmaI Enzymes that do not cut: AatII AclI AflII AflIII AgeI AleI AlfI AloI ApaLI AscI AseI AsiSI BciVI BfrBI BmtI BplI BsiWI BsrDI BsrGI BstBI BstEII BstZ17I BtgZI CspCI DraIII DrdI EcoRV FseI FspI FspAI HpaI MfeI MluI NheI NotI NruI NsiI PacI PasI PciI PmeI PsiI PspXI PvuI RsrII SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XmnI ZraI