################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BbrPI BbsI BbvCI BciVI BfrI BglII BlnI BpuAI BsaAI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII Bst1107I BstZ17I ClaI CpoI DraI DraIII Eam1105I Eco47III EcoNI EcoT22I FseI FspI HindIII HpaI KpnI KspI MamI MfeI MluI MroI MunI NcoI NdeI NheI NotI NruI NsiI NspI PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I RsrII SacII SalI SexAI SfiI SgrAI SnaBI SpeI SphI SspBI SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AocI 2202 CCTCAGG CC^T[ACGT]AGG ApaI 2198 GGGCCC GGGCC^C AspHI 1627 GAGCTC G[AT]GC[AT]^C BcgI 205 GCAACACCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1367 TGATCA T^GATCA BfaI 2128 CTAG C^TAG BlpI 596 GCTCAGC GC^T[ACGT]AGC Bpu1102I 596 GCTCAGC GC^T[ACGT]AGC BsaI 1154 GAGACC GGTCTC(1/5) BsaWI 2111 ACCGGA [AT]^CCGG[AT] BsiHKAI 1627 GAGCTC G[AT]GC[AT]^C BspHI 731 TCATGA T^CATGA BsrBI 817 GAGCGG CCGCTC(-3/-3) BsrFI 1516 GCCGGC [GA]^CCGG[CT] BssSI 102 CTCGTG CACGAG(-5/-1) BstBI 786 TTCGAA TT^CGAA BstEII 606 GGTCACC G^GT[ACGT]ACC BstPI 606 GGTCACC G^GT[ACGT]ACC BstXI 1505 CCATCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2202 CCTCAGG CC^T[ACGT]AGG CelII 596 GCTCAGC GC^T[ACGT]AGC Cfr10I 1516 GCCGGC [GA]^CCGG[CT] DrdI 826 GACAAGAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1755 CGGCCG C^GGCCG EclXI 1755 CGGCCG C^GGCCG Eco52I 1755 CGGCCG C^GGCCG Eco81I 2202 CCTCAGG CC^T[ACGT]AGG EcoO65I 606 GGTCACC G^GT[ACGT]ACC EcoRI 2155 GAATTC G^AATTC EcoRV 727 GATATC GAT^ATC FbaI 1367 TGATCA T^GATCA HincII 1054 GTCAAC GT[CT]^[GA]AC HindII 1054 GTCAAC GT[CT]^[GA]AC MaeI 2128 CTAG C^TAG MseI 1915 TTAA T^TAA NaeI 1516 GCCGGC GCC^GGC NgoMIV 1516 GCCGGC G^CCGGC NspV 786 TTCGAA TT^CGAA PshAI 1857 GACTACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 2050 CTGCAG CTGCA^G PvuI 2004 CGATCG CGAT^CG RcaI 731 TCATGA T^CATGA SacI 1627 GAGCTC GAGCT^C SapI 1956 GCTCTTC GCTCTTC(1/4) SbfI 2049 CCTGCAGG CCTGCA^GG ScaI 2147 AGTACT AGT^ACT SfuI 786 TTCGAA TT^CGAA SmlI 1126 CTTGAG C^T[CT][GA]AG Sse8387I 2049 CCTGCAGG CCTGCA^GG StuI 1620 AGGCCT AGG^CCT Tru9I 1915 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcsI 232 AAATTC [GA]^AATT[CT] 2155 GAATTC AfaI 1673 GTAC GT^AC 2148 GTAC ApoI 232 AAATTC [GA]^AATT[CT] 2155 GAATTC AspI 478 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 559 GACAAAGTC AvaI 282 CCCGGG C^[CT]CG[GA]G 2195 CCCGGG BamHI 737 GGATCC G^GATCC 897 GGATCC BbeI 675 GGCGCC GGCGC^C 2056 GGCGCC BseRI 99 CTCCTC GAGGAG(10/8) 1000 GAGGAG BsiEI 1755 CGGCCG CG[GA][CT]^CG 2004 CGATCG BsmBI 1725 CGTCTC CGTCTC(1/5) 659 GAGACG BsmI 22 GCATTC GAATGC(1/-1) 975 GCATTC BsoBI 282 CCCGGG C^[CT]CG[GA]G 2195 CCCGGG BsrDI 947 CATTGC GCAATG(2/0) 1838 GCAATG BstYI 737 GGATCC [GA]^GATC[CT] 897 GGATCC KasI 675 GGCGCC G^GCGCC 2056 GGCGCC MflI 737 GGATCC [GA]^GATC[CT] 897 GGATCC NarI 675 GGCGCC GG^CGCC 2056 GGCGCC PflFI 478 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 559 GACAAAGTC PflMI 567 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1260 CCAGGTGCTGG PpuMI 569 AGGACCT [GA]G^G[AT]CC[CT] 1812 GGGACCT RsaI 1673 GTAC GT^AC 2148 GTAC SfcI 1740 CTACAG C^T[GA][CT]AG 2050 CTGCAG SfoI 675 GGCGCC GGC^GCC 2056 GGCGCC SmaI 282 CCCGGG CCC^GGG 2195 CCCGGG TfiI 458 GAATC G^A[AT]TC 551 GATTC Tth111I 478 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 559 GACAAAGTC Van91I 567 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1260 CCAGGTGCTGG XcmI 1373 CCAGGGTGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1502 CCACCATCTTCCTGG XhoII 737 GGATCC [GA]^GATC[CT] 897 GGATCC XmaCI 282 CCCGGG C^CCGGG 2195 CCCGGG XmaI 282 CCCGGG C^CCGGG 2195 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AlwNI AvaII BalI BanI BanII BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PvuII Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I