(Linear) (Six-base) MAPSORT of: KIEE9516.seq Check: 8356 from: 1 to: 2226 KIEE9516.orf With 182 enzymes: SgfI * July 3, 2012 19:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1300 1478 2226 Size: 1300 178 748 Fragments arranged by size: 1300 748 178 AleI CACnn'nnGTG Cuts at: 0 1375 2226 Size: 1375 851 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 961 993 2226 Size: 961 32 1233 Fragments arranged by size: 1233 961 32 AlwNI CAG_nnn'CTG Cuts at: 0 74 573 1197 1266 1668 1769 2226 Size: 74 499 624 69 402 101 457 Fragments arranged by size: 624 499 457 402 101 74 69 ApaI G_GGCC'C Cuts at: 0 2202 2226 Size: 2202 24 ApoI r'AATT_y Cuts at: 0 232 2155 2226 Size: 232 1923 71 Fragments arranged by size: 1923 232 71 AvaI C'yCGr_G Cuts at: 0 282 2195 2226 Size: 282 1913 31 Fragments arranged by size: 1913 282 31 BamHI G'GATC_C Cuts at: 0 737 897 2226 Size: 737 160 1329 Fragments arranged by size: 1329 737 160 BanI G'GyrC_C Cuts at: 0 86 675 689 2056 2226 Size: 86 589 14 1367 170 Fragments arranged by size: 1367 589 170 86 14 BanII G_rGCy'C Cuts at: 0 1453 1501 1631 2202 2226 Size: 1453 48 130 571 24 Fragments arranged by size: 1453 571 130 48 24 BbeI G_GCGC'C Cuts at: 0 679 2060 2226 Size: 679 1381 166 Fragments arranged by size: 1381 679 166 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 194 228 2226 Size: 194 34 1998 Fragments arranged by size: 1998 194 34 BclI T'GATC_A Cuts at: 0 1367 2226 Size: 1367 859 BglI GCCn_nnn'nGGC Cuts at: 0 437 1619 1694 2054 2206 2226 Size: 437 1182 75 360 152 20 Fragments arranged by size: 1182 437 360 152 75 20 BlpI GC'TnA_GC Cuts at: 0 597 2226 Size: 597 1629 Bme1580I G_kGCm'C Cuts at: 0 81 91 1241 1709 2202 2226 Size: 81 10 1150 468 493 24 Fragments arranged by size: 1150 493 468 81 24 10 BmgBI CAC'GTC Cuts at: 0 1173 1344 2171 2226 Size: 1173 171 827 55 Fragments arranged by size: 1173 827 171 55 BmrI ACTGGGnnnn_n' Cuts at: 0 1251 2226 Size: 1251 975 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 378 2226 Size: 378 1848 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 51 545 677 908 1018 1645 1821 2226 Size: 51 494 132 231 110 627 176 405 Fragments arranged by size: 627 494 405 231 176 132 110 51 Bpu10I CC'TnA_GC Cuts at: 0 1309 2226 Size: 1309 917 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1147 2226 Size: 1147 1079 BsaI GGTCTCn'nnnn_ Cuts at: 0 1148 2226 Size: 1148 1078 BsaHI Gr'CG_yC Cuts at: 0 676 1041 1724 2057 2226 Size: 676 365 683 333 169 Fragments arranged by size: 683 676 365 333 169 BsaWI w'CCGG_w Cuts at: 0 2111 2226 Size: 2111 115 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 26 2226 Size: 26 2200 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 90 1015 2226 Size: 90 925 1211 Fragments arranged by size: 1211 925 90 BseYI C'CCAG_C Cuts at: 0 704 1266 2134 2226 Size: 704 562 868 92 Fragments arranged by size: 868 704 562 92 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 297 378 1456 1561 1956 2059 2226 Size: 297 81 1078 105 395 103 167 Fragments arranged by size: 1078 395 297 167 105 103 81 BsiEI CG_ry'CG Cuts at: 0 1758 2007 2226 Size: 1758 249 219 Fragments arranged by size: 1758 249 219 BsiHKAI G_wGCw'C Cuts at: 0 1631 2226 Size: 1631 595 BsmI GAATG_Cn' Cuts at: 0 22 975 2226 Size: 22 953 1251 Fragments arranged by size: 1251 953 22 BsmBI CGTCTCn'nnnn_ Cuts at: 0 653 1731 2226 Size: 653 1078 495 Fragments arranged by size: 1078 653 495 Bsp1286I G_dGCh'C Cuts at: 0 81 91 1241 1453 1501 1631 1709 2202 Size: 81 10 1150 212 48 130 78 493 Cuts at: 2202 2226 Size: 24 Fragments arranged by size: 1150 493 212 130 81 78 48 24 10 BspHI T'CATG_A Cuts at: 0 731 2226 Size: 731 1495 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 980 1300 1478 2078 2226 Size: 980 320 178 600 148 Fragments arranged by size: 980 600 320 178 148 BsrBI CCG'CTC Cuts at: 0 819 2226 Size: 819 1407 BsrDI GCAATG_nn' Cuts at: 0 946 1845 2226 Size: 946 899 381 Fragments arranged by size: 946 899 381 BsrFI r'CCGG_y Cuts at: 0 1516 2226 Size: 1516 710 BssSI C'ACGA_G Cuts at: 0 102 2226 Size: 102 2124 BstAPI GCAn_nnn'nTGC Cuts at: 0 1197 1292 2082 2226 Size: 1197 95 790 144 Fragments arranged by size: 1197 790 144 95 BstBI TT'CG_AA Cuts at: 0 787 2226 Size: 787 1439 BstEII G'GTnAC_C Cuts at: 0 606 2226 Size: 606 1620 BstXI CCAn_nnnn'nTGG Cuts at: 0 1512 2226 Size: 1512 714 BstYI r'GATC_y Cuts at: 0 737 897 2226 Size: 737 160 1329 Fragments arranged by size: 1329 737 160 Bsu36I CC'TnA_GG Cuts at: 0 2203 2226 Size: 2203 23 BtgI C'CryG_G Cuts at: 0 407 911 1023 1186 2226 Size: 407 504 112 163 1040 Fragments arranged by size: 1040 504 407 163 112 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1932 2226 Size: 1932 294 BtsI GCAGTG_nn' Cuts at: 0 2076 2226 Size: 2076 150 DrdI GACnn_nn'nnGTC Cuts at: 0 832 2226 Size: 832 1394 EaeI y'GGCC_r Cuts at: 0 743 953 1514 1536 1565 1592 1755 1943 Size: 743 210 561 22 29 27 163 188 Cuts at: 1943 2181 2226 Size: 238 45 Fragments arranged by size: 743 561 238 210 188 163 45 29 27 22 EagI C'GGCC_G Cuts at: 0 1755 2226 Size: 1755 471 EarI CTCTTCn'nnn_ Cuts at: 0 1146 1210 1402 1495 1963 2226 Size: 1146 64 192 93 468 263 Fragments arranged by size: 1146 468 263 192 93 64 EcoICRI GAG'CTC Cuts at: 0 1629 2226 Size: 1629 597 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 51 545 677 908 1018 1645 1821 2226 Size: 51 494 132 231 110 627 176 405 Fragments arranged by size: 627 494 405 231 176 132 110 51 EcoO109I rG'GnC_Cy Cuts at: 0 570 669 763 904 1665 1813 2199 2226 Size: 570 99 94 141 761 148 386 27 Fragments arranged by size: 761 570 386 148 141 99 94 27 EcoRI G'AATT_C Cuts at: 0 2155 2226 Size: 2155 71 EcoRV GAT'ATC Cuts at: 0 729 2226 Size: 729 1497 HaeII r_GCGC'y Cuts at: 0 679 1390 1933 2060 2226 Size: 679 711 543 127 166 Fragments arranged by size: 711 679 543 166 127 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 28 443 475 636 668 2226 Size: 28 415 32 161 32 1558 Fragments arranged by size: 1558 415 161 32 32 28 HincII GTy'rAC Cuts at: 0 1056 2226 Size: 1056 1170 KasI G'GCGC_C Cuts at: 0 675 2056 2226 Size: 675 1381 170 Fragments arranged by size: 1381 675 170 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1056 1757 2226 Size: 1056 701 469 Fragments arranged by size: 1056 701 469 MscI TGG'CCA Cuts at: 0 745 955 2183 2226 Size: 745 210 1228 43 Fragments arranged by size: 1228 745 210 43 MslI CAynn'nnrTG Cuts at: 0 377 1375 1852 2226 Size: 377 998 477 374 Fragments arranged by size: 998 477 377 374 MspA1I CmG'CkG Cuts at: 0 422 428 708 886 2226 Size: 422 6 280 178 1340 Fragments arranged by size: 1340 422 280 178 6 NaeI GCC'GGC Cuts at: 0 1518 2226 Size: 1518 708 NarI GG'CG_CC Cuts at: 0 676 2057 2226 Size: 676 1381 169 Fragments arranged by size: 1381 676 169 NgoMIV G'CCGG_C Cuts at: 0 1516 2226 Size: 1516 710 PflMI CCAn_nnn'nTGG Cuts at: 0 573 1266 2226 Size: 573 693 960 Fragments arranged by size: 960 693 573 PfoI T'CCnGG_A Cuts at: 0 566 654 812 2226 Size: 566 88 158 1414 Fragments arranged by size: 1414 566 158 88 PpuMI rG'GwC_Cy Cuts at: 0 570 1813 2226 Size: 570 1243 413 Fragments arranged by size: 1243 570 413 PshAI GACnn'nnGTC Cuts at: 0 1861 2226 Size: 1861 365 PspOMI G'GGCC_C Cuts at: 0 2198 2226 Size: 2198 28 PstI C_TGCA'G Cuts at: 0 2054 2226 Size: 2054 172 PvuI CG_AT'CG Cuts at: 0 2007 2226 Size: 2007 219 PvuII CAG'CTG Cuts at: 0 422 708 886 2226 Size: 422 286 178 1340 Fragments arranged by size: 1340 422 286 178 SacI G_AGCT'C Cuts at: 0 1631 2226 Size: 1631 595 SapI GCTCTTCn'nnn_ Cuts at: 0 1963 2226 Size: 1963 263 SbfI CC_TGCA'GG Cuts at: 0 2054 2226 Size: 2054 172 ScaI AGT'ACT Cuts at: 0 2149 2226 Size: 2149 77 SfcI C'TryA_G Cuts at: 0 1740 2050 2226 Size: 1740 310 176 Fragments arranged by size: 1740 310 176 SfoI GGC'GCC Cuts at: 0 677 2058 2226 Size: 677 1381 168 Fragments arranged by size: 1381 677 168 SmaI CCC'GGG Cuts at: 0 284 2197 2226 Size: 284 1913 29 Fragments arranged by size: 1913 284 29 SmlI C'TyrA_G Cuts at: 0 1126 2226 Size: 1126 1100 StuI AGG'CCT Cuts at: 0 1622 2226 Size: 1622 604 StyI C'CwwG_G Cuts at: 0 1085 1166 1656 2226 Size: 1085 81 490 570 Fragments arranged by size: 1085 570 490 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 718 2226 Size: 718 1508 TatI w'GTAC_w Cuts at: 0 2147 2226 Size: 2147 79 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1114 1146 2226 Size: 1114 32 1080 Fragments arranged by size: 1114 1080 32 Tth111I GACn'n_nGTC Cuts at: 0 481 562 2226 Size: 481 81 1664 Fragments arranged by size: 1664 481 81 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1380 1509 2226 Size: 1380 129 717 Fragments arranged by size: 1380 717 129 XmaI C'CCGG_G Cuts at: 0 282 2195 2226 Size: 282 1913 31 Fragments arranged by size: 1913 282 31 Enzymes that do cut: AarI AleI AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BcgI BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV PflMI PfoI PpuMI PshAI PspOMI PstI PvuI PvuII SacI SapI SbfI ScaI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AlfI ApaLI AscI AseI AsiSI AvrII BaeI BbsI BbvCI BciVI BfrBI BglII BmtI BsaAI BsaBI BsiWI BspEI BsrGI BssHII BstZ17I ClaI CspCI DraI DraIII EciI EcoNI FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PsiI PspXI PsrI RsrII SacII SalI SanDI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI XmnI ZraI