(Circular) (Six-base) MAPSORT of: pFN21AE0662.seq Check: 8434 from: 1 to: 5866 pFN21AE0662 5866 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2163 3676 278 Size: 53 83 186 936 627 1513 2468 Fragments arranged by size: 2468 1513 936 627 186 83 53 AccI GT'mk_AC Cuts at: 1937 3209 1937 Size: 1272 4594 Acc65I G'GTAC_C Cuts at: 3187 3187 Size: 5866 AclI AA'CG_TT Cuts at: 1563 3993 4366 1563 Size: 2430 373 3063 Fragments arranged by size: 3063 2430 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2164 2240 2294 2477 2884 2931 3929 5313 Size: 532 76 54 183 407 47 998 1384 Cuts at: 5313 5581 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 998 532 407 268 183 76 54 47 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5669 AflIII A'CryG_T Cuts at: 1203 2640 4765 5742 1203 Size: 1437 2125 977 1327 Fragments arranged by size: 2125 1437 1327 977 AgeI A'CCGG_T Cuts at: 2165 4697 2165 Size: 2532 3334 AhdI GACnn_n'nnGTC Cuts at: 4595 4595 Size: 5866 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5866 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5866 AlwNI CAG_nnn'CTG Cuts at: 2713 3609 5181 2713 Size: 896 1572 3398 Fragments arranged by size: 3398 1572 896 ApaLI G'TGCA_C Cuts at: 2444 3923 5079 2444 Size: 1479 1156 3231 Fragments arranged by size: 3231 1479 1156 ApoI r'AATT_y Cuts at: 1493 3175 3401 3553 5662 1493 Size: 1682 226 152 2109 1697 Fragments arranged by size: 2109 1697 1682 226 152 AseI AT'TA_AT Cuts at: 160 1032 4420 160 Size: 872 3388 1606 Fragments arranged by size: 3388 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 AvaI C'yCGr_G Cuts at: 1870 1943 2010 2462 2617 2759 2907 2991 3191 Size: 73 67 452 155 142 148 84 200 Cuts at: 3191 1870 Size: 4545 Fragments arranged by size: 4545 452 200 155 148 142 84 73 67 BamHI G'GATC_C Cuts at: 3196 3563 3196 Size: 367 5499 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2253 2697 3187 4642 618 Size: 325 217 627 466 444 490 1455 1842 Fragments arranged by size: 1842 1455 627 490 466 444 325 217 BanII G_rGCy'C Cuts at: 1391 2070 2340 2404 3185 1391 Size: 679 270 64 781 4072 Fragments arranged by size: 4072 781 679 270 64 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4895 BbvCI CC'TCA_GC Cuts at: 2720 2720 Size: 5866 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2538 2572 4057 4091 1903 Size: 34 601 34 1485 34 3678 Fragments arranged by size: 3678 1485 601 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3758 4968 3758 Size: 1210 4656 BclI T'GATC_A Cuts at: 1548 2727 1548 Size: 1179 4687 BfrBI ATG'CAT Cuts at: 2115 2115 Size: 5866 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2695 4477 136 Size: 107 122 71 631 605 1023 1782 1525 Fragments arranged by size: 1782 1525 1023 631 605 122 107 71 BglII A'GATC_T Cuts at: 5861 5861 Size: 5866 BlpI GC'TnA_GC Cuts at: 3283 3283 Size: 5866 Bme1580I G_kGCm'C Cuts at: 1790 2256 2448 3927 5083 1790 Size: 466 192 1479 1156 2573 Fragments arranged by size: 2573 1479 1156 466 192 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5866 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2562 3085 3597 Size: 416 53 83 399 24 1145 523 512 Cuts at: 3597 4555 442 Size: 958 1753 Fragments arranged by size: 1753 1145 958 523 512 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5866 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2354 2354 Size: 5866 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2429 2549 4526 1239 Size: 1190 120 1977 2579 Fragments arranged by size: 2579 1977 1190 120 Bpu10I CC'TnA_GC Cuts at: 2720 2720 Size: 5866 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2389 3991 4856 5154 2389 Size: 1602 865 298 3101 Fragments arranged by size: 3101 1602 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2251 2282 3003 4529 882 Size: 617 752 31 721 1526 2219 Fragments arranged by size: 2219 1526 752 721 617 31 BsaAI yAC'GTr Cuts at: 493 1204 3657 493 Size: 711 2453 2702 Fragments arranged by size: 2702 2453 711 BsaBI GATnn'nnATC Cuts at: 3002 5860 3002 Size: 2858 3008 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2160 2655 3048 3673 Size: 53 83 186 936 627 495 393 625 Cuts at: 3673 4055 4690 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 635 627 625 495 393 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2165 2700 4299 4697 4971 5118 5529 2165 Size: 535 1599 398 274 147 411 2502 Fragments arranged by size: 2502 1599 535 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5641 Fragments arranged by size: 5641 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2204 3131 3170 1186 Size: 1018 927 39 3882 Fragments arranged by size: 3882 1018 927 39 BseYI C'CCAG_C Cuts at: 5069 5565 5069 Size: 496 5370 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2025 2404 1163 Size: 573 289 379 4625 Fragments arranged by size: 4625 573 379 289 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3316 4077 5105 1540 Size: 275 186 1315 761 1028 2301 Fragments arranged by size: 2301 1315 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2340 2404 2448 2600 2651 3141 3927 4012 5083 Size: 64 44 152 51 490 786 85 1071 Cuts at: 5083 2340 Size: 3123 Fragments arranged by size: 3123 1071 786 490 152 85 64 51 44 BsmI GAATG_Cn' Cuts at: 3377 3470 3377 Size: 93 5773 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2256 2340 2404 2448 2600 2651 Size: 399 280 186 84 64 44 152 51 Cuts at: 2651 3141 3185 3927 4012 5083 1391 Size: 490 44 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 490 399 280 186 152 85 84 64 51 44 44 BspEI T'CCGG_A Cuts at: 5529 5529 Size: 5866 BspHI T'CATG_A Cuts at: 2730 3755 5485 2730 Size: 1025 1730 3111 Fragments arranged by size: 3111 1730 1025 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3221 844 Size: 2377 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2736 3753 1394 Size: 429 913 1017 3507 Fragments arranged by size: 3507 1017 913 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4361 4535 65 Size: 1183 3113 174 1396 Fragments arranged by size: 3113 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2165 2678 3036 4510 4697 1700 Size: 110 355 513 358 1474 187 2869 Fragments arranged by size: 2869 1474 513 358 355 187 110 BsrGI T'GTAC_A Cuts at: 96 2664 96 Size: 2568 3298 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5866 BssSI C'ACGA_G Cuts at: 2128 3926 4938 2128 Size: 1798 1012 3056 Fragments arranged by size: 3056 1798 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5866 BstBI TT'CG_AA Cuts at: 4670 4670 Size: 5866 BstEII G'GTnAC_C Cuts at: 1184 2362 1184 Size: 1178 4688 BstXI CCAn_nnnn'nTGG Cuts at: 2575 2575 Size: 5866 BstYI r'GATC_y Cuts at: 1972 2076 2955 3196 3563 3948 3965 5417 5503 Size: 104 879 241 367 385 17 1452 86 Cuts at: 5503 5515 5861 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 879 385 367 346 241 104 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5314 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2125 487 Size: 683 56 899 4228 Fragments arranged by size: 4228 899 683 56 BtsI GCAGTG_nn' Cuts at: 2021 2104 3382 4176 4196 2021 Size: 83 1278 794 20 3691 Fragments arranged by size: 3691 1278 794 83 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5866 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5831 DraI TTT'AAA Cuts at: 3170 3170 Size: 5866 DraIII CAC_nnn'GTG Cuts at: 3079 3079 Size: 5866 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4873 809 Size: 507 3557 1802 Fragments arranged by size: 3557 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2676 3070 3150 Size: 54 1425 50 275 18 846 394 80 Cuts at: 3150 3313 4202 5831 8 Size: 163 889 1629 43 Fragments arranged by size: 1629 1425 889 846 394 275 163 80 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3313 1537 Size: 275 1501 4090 Fragments arranged by size: 4090 1501 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2321 2838 3796 1353 Size: 968 517 958 3423 Fragments arranged by size: 3423 968 958 517 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4453 4827 4973 387 Size: 1064 138 2864 374 146 1280 Fragments arranged by size: 2864 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2338 2402 2338 Size: 64 5802 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2164 2240 2294 2429 2477 2549 2884 Size: 393 532 76 54 135 48 72 335 Cuts at: 2884 2931 3929 4526 5313 5581 1239 Size: 47 998 597 787 268 1524 Fragments arranged by size: 1524 998 787 597 532 393 335 268 135 76 72 54 48 47 EcoNI CCTnn'n_nnAGG Cuts at: 2240 3152 2240 Size: 912 4954 EcoO109I rG'GnC_Cy Cuts at: 2452 2562 2791 2452 Size: 110 229 5527 Fragments arranged by size: 5527 229 110 EcoRI G'AATT_C Cuts at: 3175 3553 3175 Size: 378 5488 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2383 2383 Size: 5866 FspI TGC'GCA Cuts at: 2240 4372 2240 Size: 2132 3734 HaeII r_GCGC'y Cuts at: 1678 5013 1678 Size: 3335 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2759 2791 4507 4539 4581 Size: 32 135 32 1187 32 1716 32 42 Cuts at: 4581 4613 1373 Size: 32 2626 Fragments arranged by size: 2626 1716 1187 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3210 3456 669 Size: 988 281 1272 246 3079 Fragments arranged by size: 3079 1272 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5866 HpaI GTT'AAC Cuts at: 3456 3456 Size: 5866 KpnI G_GTAC'C Cuts at: 3191 3191 Size: 5866 MfeI C'AATT_G Cuts at: 3465 3465 Size: 5866 MluI A'CGCG_T Cuts at: 5742 5742 Size: 5866 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4980 5164 1305 Size: 3675 184 2007 Fragments arranged by size: 3675 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3072 10 Size: 54 1425 343 1240 2804 Fragments arranged by size: 2804 1425 1240 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3824 4183 4342 518 Size: 648 2658 359 159 2042 Fragments arranged by size: 2658 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2272 2527 2840 3282 3959 5107 5352 1606 Size: 666 255 313 442 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 666 442 313 255 245 NaeI GCC'GGC Cuts at: 2680 3038 2680 Size: 358 5508 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5314 NdeI CA'TA_TG Cuts at: 387 387 Size: 5866 NgoMIV G'CCGG_C Cuts at: 2678 3036 2678 Size: 358 5508 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5866 NotI GC'GGCC_GC Cuts at: 3313 3313 Size: 5866 NsiI A_TGCA'T Cuts at: 2117 2117 Size: 5866 NspI r_CATG'y Cuts at: 1132 1229 2644 3224 4769 1132 Size: 97 1415 580 1545 2229 Fragments arranged by size: 2229 1545 1415 580 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5866 PciI A'CATG_T Cuts at: 2640 4765 2640 Size: 2125 3741 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5866 PmeI GTTT'AAAC Cuts at: 3170 3170 Size: 5866 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3935 3967 5448 5480 3935 Size: 32 1481 32 4321 Fragments arranged by size: 4321 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2452 2562 2791 2452 Size: 110 229 5527 Fragments arranged by size: 5527 229 110 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5866 PsiI TTA'TAA Cuts at: 3436 3436 Size: 5866 PstI C_TGCA'G Cuts at: 830 3218 830 Size: 2388 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5866 PvuII CAG'CTG Cuts at: 2272 2527 2272 Size: 255 5611 SacI G_AGCT'C Cuts at: 2340 2404 2340 Size: 64 5802 SalI G'TCGA_C Cuts at: 1936 3208 1936 Size: 1272 4594 SanDI GG'GwC_CC Cuts at: 2562 2562 Size: 5866 SbfI CC_TGCA'GG Cuts at: 3218 3218 Size: 5866 ScaI AGT'ACT Cuts at: 2876 4114 5826 2876 Size: 1238 1712 2916 Fragments arranged by size: 2916 1712 1238 SexAI A'CCwGG_T Cuts at: 2793 2793 Size: 5866 SfcI C'TryA_G Cuts at: 826 1045 3214 4349 5030 5221 826 Size: 219 2169 1135 681 191 1471 Fragments arranged by size: 2169 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 SgrAI Cr'CCGG_yG Cuts at: 1700 2165 1700 Size: 465 5401 SmaI CCC'GGG Cuts at: 1872 2012 2619 2909 2993 3193 1872 Size: 140 607 290 84 200 4545 Fragments arranged by size: 4545 607 290 200 140 84 SmlI C'TyrA_G Cuts at: 820 1017 1943 2368 3970 4871 5133 820 Size: 197 926 425 1602 901 262 1553 Fragments arranged by size: 1602 1553 926 901 425 262 197 SnaBI TAC'GTA Cuts at: 493 3657 493 Size: 3164 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5866 SphI G_CATG'C Cuts at: 1132 3224 1132 Size: 2092 3774 SspI AAT'ATT Cuts at: 5 52 3790 5 Size: 47 3738 2081 Fragments arranged by size: 3738 2081 47 StuI AGG'CCT Cuts at: 1517 2849 1517 Size: 1332 4534 StyI C'CwwG_G Cuts at: 513 1065 2850 3129 3305 513 Size: 552 1785 279 176 3074 Fragments arranged by size: 3074 1785 552 279 176 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3067 3142 3893 3910 4063 4248 4693 Size: 1255 1487 75 751 17 153 185 445 Cuts at: 4693 325 Size: 1498 Fragments arranged by size: 1498 1487 1255 751 445 185 153 75 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2664 2874 4112 Size: 274 80 33 51 1443 687 210 1238 Cuts at: 4112 5824 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1238 687 274 210 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5826 5858 5826 Size: 32 5834 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5866 XbaI T'CTAG_A Cuts at: 3202 3202 Size: 5866 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2077 2077 Size: 5866 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5866 XmaI C'CCGG_G Cuts at: 1870 2010 2617 2907 2991 3191 1870 Size: 140 607 290 84 200 4545 Fragments arranged by size: 4545 607 290 200 140 84 XmnI GAAnn'nnTTC Cuts at: 3995 3995 Size: 5866 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2161 3674 276 Size: 53 83 186 936 627 1513 2468 Fragments arranged by size: 2468 1513 936 627 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI