Restriction map for
pFN21AE3473
(Circular) (Six-base) MAPSORT of: pFN21AE3473.seq Check: 3608 from: 1 to: 9316
pFN21AE3473 9316 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4029 4029
Size: 9316
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7126 278
Size: 53 83 186 936 5590 2468
Fragments arranged by size:
5590 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2061 2082 2346 6258 6659 1937
Size: 124 21 264 3912 401 4594
Fragments arranged by size:
4594 3912 401 264 124 21
Acc65I G'GTAC_C
Cuts at: 6637 6637
Size: 9316
AclI AA'CG_TT
Cuts at: 1563 6615 7443 7816 1563
Size: 5052 828 373 3063
Fragments arranged by size:
5052 3063 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2051 2783 2818 2921 3902 5474 5980 6161
Size: 419 732 35 103 981 1572 506 181
Cuts at: 6161 7379 8763 9031 1632
Size: 1218 1384 268 1917
Fragments arranged by size:
1917 1572 1384 1218 981 732 506 419
268 181 103 35
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9119
AflIII A'CryG_T
Cuts at: 1203 6531 8215 9192 1203
Size: 5328 1684 977 1327
Fragments arranged by size:
5328 1684 1327 977
AgeI A'CCGG_T
Cuts at: 6387 8147 6387
Size: 1760 7556
AhdI GACnn_n'nnGTC
Cuts at: 4009 4735 8045 4009
Size: 726 3310 5280
Fragments arranged by size:
5280 3310 726
AleI CACnn'nnGTG
Cuts at: 1166 4023 1166
Size: 2857 6459
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2891 4776 1184
Size: 1707 1885 5724
Fragments arranged by size:
5724 1885 1707
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2591 2623 2591
Size: 32 9284
AlwNI CAG_nnn'CTG
Cuts at: 3490 3499 3544 3580 3609 3736 3880 4219 5114
Size: 9 45 36 29 127 144 339 895
Cuts at: 5114 5416 5576 6493 7059 8631 3490
Size: 302 160 917 566 1572 4175
Fragments arranged by size:
4175 1572 917 895 566 339 302 160
144 127 45 36 29 9
ApaI G_GGCC'C
Cuts at: 3083 5060 6029 6201 6404 3083
Size: 1977 969 172 203 5995
Fragments arranged by size:
5995 1977 969 203 172
ApaLI G'TGCA_C
Cuts at: 6527 7373 8529 6527
Size: 846 1156 7314
Fragments arranged by size:
7314 1156 846
ApoI r'AATT_y
Cuts at: 1493 6625 6851 7003 9112 1493
Size: 5132 226 152 2109 1697
Fragments arranged by size:
5132 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7870 160
Size: 872 6838 1606
Fragments arranged by size:
6838 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9316
AvaI C'yCGr_G
Cuts at: 1870 1943 2369 2402 3269 5066 5926 6029 6243
Size: 73 426 33 867 1797 860 103 214
Cuts at: 6243 6641 1870
Size: 398 4545
Fragments arranged by size:
4545 1797 867 860 426 398 214 103
73 33
BamHI G'GATC_C
Cuts at: 6646 7013 6646
Size: 367 8949
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2276 2867 3030 3240 3436
Size: 325 217 627 489 591 163 210 196
Cuts at: 3436 4034 4246 4353 4375 5930 6637 8092 618
Size: 598 212 107 22 1555 707 1455 1842
Fragments arranged by size:
1842 1555 1455 707 627 598 591 489
325 217 212 210 196 163 107 22
BanII G_rGCy'C
Cuts at: 1391 2055 2394 2892 2909 3083 3972 4130 4137
Size: 664 339 498 17 174 889 158 7
Cuts at: 4137 4170 4454 4484 4587 5060 5332 5462 5589
Size: 33 284 30 103 473 272 130 127
Cuts at: 5589 5850 6029 6050 6089 6201 6404 6635 1391
Size: 261 179 21 39 112 203 231 4072
Fragments arranged by size:
4072 889 664 498 473 339 284 272
261 231 203 179 174 158 130 127
112 103 39 33 30 21 17 7
BbeI G_GCGC'C
Cuts at: 3034 3244 3440 4038 3034
Size: 210 196 598 8312
Fragments arranged by size:
8312 598 210 196
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 8345
BbvCI CC'TCA_GC
Cuts at: 3132 3602 4346 5804 6320 3132
Size: 470 744 1458 516 6128
Fragments arranged by size:
6128 1458 744 516 470
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5234 5268 7507 7541 1903
Size: 34 3297 34 2239 34 3678
Fragments arranged by size:
3678 3297 2239 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7208 8418 7208
Size: 1210 8106
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9316
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3087 4269 4384
Size: 107 122 71 631 605 1415 1182 115
Cuts at: 4384 4691 4889 5771 5867 6239 6344 7927 136
Size: 307 198 882 96 372 105 1583 1525
Fragments arranged by size:
1583 1525 1415 1182 882 631 605 372
307 198 122 115 107 105 96 71
BglII A'GATC_T
Cuts at: 3714 3891 9311 3714
Size: 177 5420 3719
Fragments arranged by size:
5420 3719 177
BlpI GC'TnA_GC
Cuts at: 2049 3591 4181 4416 4478 4616 6056 6221 6733
Size: 1542 590 235 62 138 1440 165 512
Cuts at: 6733 2049
Size: 4632
Fragments arranged by size:
4632 1542 1440 590 512 235 165 138
62
Bme1580I G_kGCm'C
Cuts at: 1790 2279 3083 4251 4380 5060 5472 5539 5933
Size: 489 804 1168 129 680 412 67 394
Cuts at: 5933 6029 6201 6404 6531 7377 8533 1790
Size: 96 172 203 127 846 1156 2573
Fragments arranged by size:
2573 1168 1156 846 804 680 489 412
394 203 172 129 127 96 67
BmgBI CAC'GTC
Cuts at: 1318 4267 1318
Size: 2949 6367
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3514 3844 4207
Size: 416 53 83 399 24 2097 330 363
Cuts at: 4207 4701 7047 8005 442
Size: 494 2346 958 1753
Fragments arranged by size:
2346 2097 1753 958 494 416 399 363
330 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9316
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2413 5590 5866 2413
Size: 3177 276 5863
Fragments arranged by size:
5863 3177 276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2039 2501 3779 3872 3938 4073 4100 4304
Size: 800 462 1278 93 66 135 27 204
Cuts at: 4304 4548 4894 4899 5011 5170 5438 5861 7976
Size: 244 346 5 112 159 268 423 2115
Cuts at: 7976 1239
Size: 2579
Fragments arranged by size:
2579 2115 1278 800 462 423 346 268
244 204 159 135 112 93 66 27
5
Bpu10I CC'TnA_GC
Cuts at: 3132 3602 4346 4358 5804 6044 6107 6320 3132
Size: 470 744 12 1446 240 63 213 6128
Fragments arranged by size:
6128 1446 744 470 240 213 63 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2141 2875 3788 3965 4135 5320 5486 6248 7441
Size: 734 913 177 170 1185 166 762 1193
Cuts at: 7441 8306 8604 2141
Size: 865 298 2853
Fragments arranged by size:
2853 1193 1185 913 865 762 734 298
177 170 166
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2114 2411 2585 5076 5376 5438 7979
Size: 617 615 297 174 2491 300 62 2541
Cuts at: 7979 882
Size: 2219
Fragments arranged by size:
2541 2491 2219 617 615 300 297 174
62
BsaAI yAC'GTr
Cuts at: 493 1204 6532 7107 493
Size: 711 5328 575 2702
Fragments arranged by size:
5328 2702 711 575
BsaBI GATnn'nnATC
Cuts at: 2091 9310 2091
Size: 7219 2097
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2556 3031 3111 3241
Size: 53 83 186 936 1023 475 80 130
Cuts at: 3241 3437 4005 4035 4062 5291 6093 6347 6368
Size: 196 568 30 27 1229 802 254 21
Cuts at: 6368 7123 7505 8140 275
Size: 755 382 635 1451
Fragments arranged by size:
1451 1229 1023 936 802 755 635 568
475 382 254 196 186 130 83 80
53 30 27 21
BsaWI w'CCGG_w
Cuts at: 6387 7749 8147 8421 8568 8979 6387
Size: 1362 398 274 147 411 6724
Fragments arranged by size:
6724 1362 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3845 3875 4277 4307 5006
Size: 30 165 30 2442 30 402 30 699
Cuts at: 5006 5036 5075 5105 5372 5402 6446 6476 6600
Size: 30 39 30 267 30 1044 30 124
Cuts at: 6600 6630 1178
Size: 30 3864
Fragments arranged by size:
3864 2442 1044 699 402 267 165 124
39 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3334 3590 3641 3833 4711 5014 5146 5579
Size: 2148 256 51 192 878 303 132 433
Cuts at: 5579 5858 6308 6466 1186
Size: 279 450 158 4036
Fragments arranged by size:
4036 2148 878 450 433 303 279 256
192 158 132 51
BseYI C'CCAG_C
Cuts at: 3547 3573 3664 4454 4676 4778 5294 5588 6134
Size: 26 91 790 222 102 516 294 546
Cuts at: 6134 8519 9015 3547
Size: 2385 496 3848
Fragments arranged by size:
3848 2385 790 546 516 496 294 222
102 91 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2930 3282 3296 3425 4001 4058 4075
Size: 573 1194 352 14 129 576 57 17
Cuts at: 4075 4646 5935 1163
Size: 571 1289 4544
Fragments arranged by size:
4544 1289 1194 576 573 571 352 129
57 17 14
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2309 2959 4273 4277 5266 6039
Size: 275 186 308 650 1314 4 989 773
Cuts at: 6039 6300 6513 6766 7527 8555 1540
Size: 261 213 253 761 1028 2301
Fragments arranged by size:
2301 1314 1028 989 773 761 650 308
275 261 253 213 186 4
BsiHKAI G_wGCw'C
Cuts at: 2517 2892 3972 4137 4170 4422 4587 4703 5462
Size: 375 1080 165 33 252 165 116 759
Cuts at: 5462 5502 5599 6050 6531 7377 7462 8533 2517
Size: 40 97 451 481 846 85 1071 3300
Fragments arranged by size:
3300 1080 1071 846 759 481 451 375
252 165 165 116 97 85 40 33
BsmI GAATG_Cn'
Cuts at: 4405 4650 6827 6920 4405
Size: 245 2177 93 6801
Fragments arranged by size:
6801 2177 245 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3177 3177
Size: 9316
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2279 2394 2517 2892 2909 3083
Size: 399 265 224 115 123 375 17 174
Cuts at: 3083 3972 4130 4137 4170 4251 4380 4422 4454
Size: 889 158 7 33 81 129 42 32
Cuts at: 4454 4484 4587 4703 5060 5332 5462 5472 5502
Size: 30 103 116 357 272 130 10 30
Cuts at: 5502 5539 5589 5599 5850 5933 6029 6050 6089
Size: 37 50 10 251 83 96 21 39
Cuts at: 6089 6201 6404 6531 6635 7377 7462 8533 1391
Size: 112 203 127 104 742 85 1071 2174
Fragments arranged by size:
2174 1071 889 742 399 375 357 272
265 251 224 203 174 158 130 129
127 123 116 115 112 104 103 96
85 83 81 50 42 39 37 33
32 30 30 21 17 10 10 7
BspEI T'CCGG_A
Cuts at: 8979 8979
Size: 9316
BspHI T'CATG_A
Cuts at: 2107 7205 8935 2107
Size: 5098 1730 2488
Fragments arranged by size:
5098 2488 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2332 2952 3498 4029 4799 5094 6671 844
Size: 1488 620 546 531 770 295 1577 3489
Fragments arranged by size:
3489 1577 1488 770 620 546 531 295
BsrBI CCG'CTC
Cuts at: 1394 1823 2496 2770 3274 4615 5512 5873 6225
Size: 429 673 274 504 1341 897 361 352
Cuts at: 6225 7203 1394
Size: 978 3507
Fragments arranged by size:
3507 1341 978 897 673 504 429 361
352 274
BsrDI GCAATG_nn'
Cuts at: 65 1248 7811 7985 65
Size: 1183 6563 174 1396
Fragments arranged by size:
6563 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2077 3098 4068 4185 4272 4387 4888
Size: 110 267 1021 970 117 87 115 501
Cuts at: 4888 5265 5699 6038 6387 6470 6508 7960 8147
Size: 377 434 339 349 83 38 1452 187
Cuts at: 8147 1700
Size: 2869
Fragments arranged by size:
2869 1452 1021 970 501 434 377 349
339 267 187 117 115 110 87 83
38
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9316
BssHII G'CGCG_C
Cuts at: 1925 3774 4561 1925
Size: 1849 787 6680
Fragments arranged by size:
6680 1849 787
BssSI C'ACGA_G
Cuts at: 2707 7376 8388 2707
Size: 4669 1012 3635
Fragments arranged by size:
4669 3635 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3305 3544 4969 794
Size: 2511 239 1425 5141
Fragments arranged by size:
5141 2511 1425 239
BstBI TT'CG_AA
Cuts at: 8120 8120
Size: 9316
BstEII G'GTnAC_C
Cuts at: 1184 2359 1184
Size: 1175 8141
BstXI CCAn_nnnn'nTGG
Cuts at: 4157 4778 5531 4157
Size: 621 753 7942
Fragments arranged by size:
7942 753 621
BstYI r'GATC_y
Cuts at: 1972 2152 3365 3480 3714 3891 5110 6646 7013
Size: 180 1213 115 234 177 1219 1536 367
Cuts at: 7013 7398 7415 8867 8953 8965 9311 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1536 1452 1219 1213 385 367 346
234 180 177 115 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2745 4485 2745
Size: 1740 7576
BtgI C'CryG_G
Cuts at: 513 1065 2004 2040 2066 2595 3117 3781 4493
Size: 552 939 36 26 529 522 664 712
Cuts at: 4493 4771 4935 5218 5239 5391 5973 6157 6201
Size: 278 164 283 21 152 582 184 44
Cuts at: 6201 6465 6570 513
Size: 264 105 3259
Fragments arranged by size:
3259 939 712 664 582 552 529 522
283 278 264 184 164 152 105 44
36 26 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8577
Fragments arranged by size:
8577 683 56
BtsI GCAGTG_nn'
Cuts at: 3258 3607 3910 4546 5173 5599 6215 6832 7626
Size: 349 303 636 627 426 616 617 794
Cuts at: 7626 7646 3258
Size: 20 4928
Fragments arranged by size:
4928 794 636 627 617 616 426 349
303 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9316
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9281
DraI TTT'AAA
Cuts at: 6620 6620
Size: 9316
DraIII CAC_nnn'GTG
Cuts at: 2359 4375 2359
Size: 2016 7300
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6254 6388 8323 809
Size: 507 4938 134 1935 1802
Fragments arranged by size:
4938 1935 1802 507 134
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2069 2306 2577
Size: 54 1425 50 275 18 239 237 271
Cuts at: 2577 3063 3096 3226 3784 4147 4270 4274 4332
Size: 486 33 130 558 363 123 4 58
Cuts at: 4332 4659 4833 4890 5263 5763 5868 5970 6036
Size: 327 174 57 373 500 105 102 66
Cuts at: 6036 6271 6297 6468 6510 6763 7652 9281 8
Size: 235 26 171 42 253 889 1629 43
Fragments arranged by size:
1629 1425 889 558 500 486 373 363
327 275 271 253 239 237 235 174
171 130 123 105 102 66 58 57
54 50 43 42 33 26 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2306 4270 4274 5263 6036 6297 6510
Size: 275 494 1964 4 989 773 261 213
Cuts at: 6510 6763 1537
Size: 253 4090
Fragments arranged by size:
4090 1964 989 773 494 275 261 253
213 4
EarI CTCTTCn'nnn_
Cuts at: 1353 2676 3132 3878 3944 4529 5450 6018 6287
Size: 1323 456 746 66 585 921 568 269
Cuts at: 6287 7246 1353
Size: 959 3423
Fragments arranged by size:
3423 1323 959 921 746 585 568 456
269 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3709 5284 5788 5789 6364 7903
Size: 1064 138 2120 1575 504 1 575 1539
Cuts at: 7903 8277 8423 387
Size: 374 146 1280
Fragments arranged by size:
2120 1575 1539 1280 1064 575 504 374
146 138 1
EcoICRI GAG'CTC
Cuts at: 2890 3970 4135 4168 4585 5460 6048 2890
Size: 1080 165 33 417 875 588 6158
Fragments arranged by size:
6158 1080 875 588 417 165 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2051 2501 2783 2818 2921 3779
Size: 393 407 12 450 282 35 103 858
Cuts at: 3779 3872 3902 3938 4073 4100 4304 4548 4894
Size: 93 30 36 135 27 204 244 346
Cuts at: 4894 4899 5011 5170 5438 5474 5861 5980 6161
Size: 5 112 159 268 36 387 119 181
Cuts at: 6161 7379 7976 8763 9031 1239
Size: 1218 597 787 268 1524
Fragments arranged by size:
1524 1218 858 787 597 450 407 393
387 346 282 268 268 244 204 181
159 135 119 112 103 93 36 36
35 30 27 12 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2339 4235 4361 2339
Size: 1896 126 7294
Fragments arranged by size:
7294 1896 126
EcoO109I rG'GnC_Cy
Cuts at: 3079 3877 4489 4512 4549 4845 4941 5056 5965
Size: 798 612 23 37 296 96 115 909
Cuts at: 5965 6025 6026 6401 3079
Size: 60 1 375 5994
Fragments arranged by size:
5994 909 798 612 375 296 115 96
60 37 23 1
EcoRI G'AATT_C
Cuts at: 6625 7003 6625
Size: 378 8938
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5752 5752
Size: 9316
FseI GG_CCGG'CC
Cuts at: 3102 4276 6042 3102
Size: 1174 1766 6376
Fragments arranged by size:
6376 1766 1174
FspI TGC'GCA
Cuts at: 7822 7822
Size: 9316
HaeII r_GCGC'y
Cuts at: 1678 3034 3244 3440 3680 4038 5217 5326 8463
Size: 1356 210 196 240 358 1179 109 3137
Cuts at: 8463 1678
Size: 2531
Fragments arranged by size:
3137 2531 1356 1179 358 240 210 196
109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2660 2692 3313 3345 3995
Size: 32 135 32 1088 32 621 32 650
Cuts at: 3995 4027 4277 4309 4721 4753 4892 4924 5075
Size: 32 250 32 412 32 139 32 151
Cuts at: 5075 5107 7957 7989 8031 8063 1373
Size: 32 2850 32 42 32 2626
Fragments arranged by size:
2850 2626 1088 650 621 412 250 151
139 135 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6259 6660 6906 669
Size: 988 281 4321 401 246 3079
Fragments arranged by size:
4321 3079 988 401 281 246
HindIII A'AGCT_T
Cuts at: 748 2203 3461 748
Size: 1455 1258 6603
Fragments arranged by size:
6603 1455 1258
HpaI GTT'AAC
Cuts at: 6906 6906
Size: 9316
KasI G'GCGC_C
Cuts at: 3030 3240 3436 4034 3030
Size: 210 196 598 8312
Fragments arranged by size:
8312 598 210 196
KpnI G_GTAC'C
Cuts at: 6641 6641
Size: 9316
MfeI C'AATT_G
Cuts at: 6915 6915
Size: 9316
MluI A'CGCG_T
Cuts at: 9192 9192
Size: 9316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3729 5775 6586 8430 8614 1305
Size: 2424 2046 811 1844 184 2007
Fragments arranged by size:
2424 2046 2007 1844 811 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4661 4835 5765 6273 10
Size: 54 1425 343 2829 174 930 508 3053
Fragments arranged by size:
3053 2829 1425 930 508 343 174 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2097 2550 2873 2879 4023 7274 7633
Size: 648 931 453 323 6 1144 3251 359
Cuts at: 7633 7792 518
Size: 159 2042
Fragments arranged by size:
3251 2042 1144 931 648 453 359 323
159 6
MspA1I CmG'CkG
Cuts at: 1606 2074 2266 2761 3409 3427 3736 3757 3783
Size: 468 192 495 648 18 309 21 26
Cuts at: 3783 3799 3829 3958 4495 4578 4842 5785 5975
Size: 16 30 129 537 83 264 943 190
Cuts at: 5975 6055 6121 6159 6190 6324 6542 6732 7409
Size: 80 66 38 31 134 218 190 677
Cuts at: 7409 8557 8802 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 943 677 648 537 495 468
309 264 245 218 192 190 190 134
129 83 80 66 38 31 30 26
21 18 16
NaeI GCC'GGC
Cuts at: 3100 4070 4187 4274 4389 4890 5267 5701 6040
Size: 970 117 87 115 501 377 434 339
Cuts at: 6040 6472 6510 3100
Size: 432 38 5906
Fragments arranged by size:
5906 970 501 434 432 377 339 117
115 87 38
NarI GG'CG_CC
Cuts at: 3031 3241 3437 4035 3031
Size: 210 196 598 8312
Fragments arranged by size:
8312 598 210 196
NcoI C'CATG_G
Cuts at: 513 1065 2004 2595 3117 5391 6570 513
Size: 552 939 591 522 2274 1179 3259
Fragments arranged by size:
3259 2274 1179 939 591 552 522
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9316
NgoMIV G'CCGG_C
Cuts at: 3098 4068 4185 4272 4387 4888 5265 5699 6038
Size: 970 117 87 115 501 377 434 339
Cuts at: 6038 6470 6508 3098
Size: 432 38 5906
Fragments arranged by size:
5906 970 501 434 432 377 339 117
115 87 38
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9316
NotI GC'GGCC_GC
Cuts at: 6763 6763
Size: 9316
NspI r_CATG'y
Cuts at: 1132 1229 2218 2645 6674 8219 1132
Size: 97 989 427 4029 1545 2229
Fragments arranged by size:
4029 2229 1545 989 427 97
PasI CC'CwG_GG
Cuts at: 1348 4039 4508 5053 1348
Size: 2691 469 545 5611
Fragments arranged by size:
5611 2691 545 469
PciI A'CATG_T
Cuts at: 8215 8215
Size: 9316
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2130 3580 4219 4243 5416 1108
Size: 1022 1450 639 24 1173 5008
Fragments arranged by size:
5008 1450 1173 1022 639 24
PfoI T'CCnGG_A
Cuts at: 2148 6307 2148
Size: 4159 5157
PmeI GTTT'AAAC
Cuts at: 6620 6620
Size: 9316
PmlI CAC'GTG
Cuts at: 6532 6532
Size: 9316
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7385 7417 8898 8930 7385
Size: 32 1481 32 7771
Fragments arranged by size:
7771 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4489 4941 4489
Size: 452 8864
PshAI GACnn'nnGTC
Cuts at: 1613 2032 1613
Size: 419 8897
PsiI TTA'TAA
Cuts at: 6886 6886
Size: 9316
PspOMI G'GGCC_C
Cuts at: 3079 5056 6025 6197 6400 3079
Size: 1977 969 172 203 5995
Fragments arranged by size:
5995 1977 969 203 172
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3479 3511 3479
Size: 32 9284
PstI C_TGCA'G
Cuts at: 830 2340 3459 3495 3540 3828 3927 3978 4722
Size: 1510 1119 36 45 288 99 51 744
Cuts at: 4722 5790 6450 6668 830
Size: 1068 660 218 3478
Fragments arranged by size:
3478 1510 1119 1068 744 660 288 218
99 51 45 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9316
PvuII CAG'CTG
Cuts at: 2266 2761 3736 3757 3799 3829 6121 2266
Size: 495 975 21 42 30 2292 5461
Fragments arranged by size:
5461 2292 975 495 42 30 21
SacI G_AGCT'C
Cuts at: 2892 3972 4137 4170 4587 5462 6050 2892
Size: 1080 165 33 417 875 588 6158
Fragments arranged by size:
6158 1080 875 588 417 165 33
SacII CC_GC'GG
Cuts at: 3784 4496 5976 6160 3784
Size: 712 1480 184 6940
Fragments arranged by size:
6940 1480 712 184
SalI G'TCGA_C
Cuts at: 1936 6257 6658 1936
Size: 4321 401 4594
Fragments arranged by size:
4594 4321 401
SanDI GG'GwC_CC
Cuts at: 4941 4941
Size: 9316
SapI GCTCTTCn'nnn_
Cuts at: 3878 3944 4529 5450 3878
Size: 66 585 921 7744
Fragments arranged by size:
7744 921 585 66
SbfI CC_TGCA'GG
Cuts at: 3540 6450 6668 3540
Size: 2910 218 6188
Fragments arranged by size:
6188 2910 218
ScaI AGT'ACT
Cuts at: 7564 9276 7564
Size: 1712 7604
SexAI A'CCwGG_T
Cuts at: 2430 2430
Size: 9316
SfcI C'TryA_G
Cuts at: 826 1045 2336 3361 3455 3491 3536 3824 3923
Size: 219 1291 1025 94 36 45 288 99
Cuts at: 3923 3974 4718 5786 6446 6485 6664 7799 8480
Size: 51 744 1068 660 39 179 1135 681
Cuts at: 8480 8671 826
Size: 191 1471
Fragments arranged by size:
1471 1291 1135 1068 1025 744 681 660
288 219 191 179 99 94 51 45
39 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 6239 6239
Size: 9316
SfoI GGC'GCC
Cuts at: 3032 3242 3438 4036 3032
Size: 210 196 598 8312
Fragments arranged by size:
8312 598 210 196
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9316
SgrAI Cr'CCGG_yG
Cuts at: 1700 5699 1700
Size: 3999 5317
SmaI CCC'GGG
Cuts at: 1872 5928 6245 6643 1872
Size: 4056 317 398 4545
Fragments arranged by size:
4545 4056 398 317
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2156 2854 3803 3980 4114 5299
Size: 197 926 213 698 949 177 134 1185
Cuts at: 5299 5501 6263 7420 8321 8583 820
Size: 202 762 1157 901 262 1553
Fragments arranged by size:
1553 1185 1157 949 926 901 762 698
262 213 202 197 177 134
SnaBI TAC'GTA
Cuts at: 493 7107 493
Size: 6614 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9316
SphI G_CATG'C
Cuts at: 1132 2218 2645 6674 1132
Size: 1086 427 4029 3774
Fragments arranged by size:
4029 3774 1086 427
SrfI GCCC'GGGC
Cuts at: 5928 5928
Size: 9316
SspI AAT'ATT
Cuts at: 5 52 7240 5
Size: 47 7188 2081
Fragments arranged by size:
7188 2081 47
StuI AGG'CCT
Cuts at: 1517 6522 1517
Size: 5005 4311
StyI C'CwwG_G
Cuts at: 513 1065 2004 2595 3117 4425 4848 4896 5391
Size: 552 939 591 522 1308 423 48 495
Cuts at: 5391 5655 6237 6570 6755 513
Size: 264 582 333 185 3074
Fragments arranged by size:
3074 1308 939 591 582 552 522 495
423 333 264 185 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2378 2480 3572 3656 7343 7360 7513
Size: 1255 798 102 1092 84 3687 17 153
Cuts at: 7513 7698 8143 325
Size: 185 445 1498
Fragments arranged by size:
3687 1498 1255 1092 798 445 185 153
102 84 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2235 3522 7562
Size: 274 80 33 51 1443 258 1287 4040
Cuts at: 7562 9274 96
Size: 1712 138
Fragments arranged by size:
4040 1712 1443 1287 274 258 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4693 4725 5006 5038 6521 6553 9276 9308 4693
Size: 32 281 32 1483 32 2723 32 4701
Fragments arranged by size:
4701 2723 1483 281 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3325 1316
Size: 2009 7307
XbaI T'CTAG_A
Cuts at: 3466 6652 3466
Size: 3186 6130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2878 3427 3847 2878
Size: 549 420 8347
Fragments arranged by size:
8347 549 420
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9316
XmaI C'CCGG_G
Cuts at: 1870 5926 6243 6641 1870
Size: 4056 317 398 4545
Fragments arranged by size:
4545 4056 398 317
XmnI GAAnn'nnTTC
Cuts at: 7445 7445
Size: 9316
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7124 276
Size: 53 83 186 936 5590 2468
Fragments arranged by size:
5590 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV
FspAI NruI NsiI PacI PspXI RsrII SwaI