(Linear) (Six-base) MAPSORT of: KIBB6286.seq Check: 4037 from: 1 to: 2352 KIBB6286.orf With 182 enzymes: SgfI * July 3, 2012 16:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1593 1787 2154 2352 Size: 1593 194 367 198 Fragments arranged by size: 1593 367 198 194 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 129 819 1596 2352 Size: 129 690 777 756 Fragments arranged by size: 777 756 690 129 AfeI AGC'GCT Cuts at: 0 1579 2352 Size: 1579 773 AflIII A'CryG_T Cuts at: 0 1640 2352 Size: 1640 712 AleI CACnn'nnGTG Cuts at: 0 1457 2352 Size: 1457 895 AlwNI CAG_nnn'CTG Cuts at: 0 510 886 1023 1186 1212 1257 2352 Size: 510 376 137 163 26 45 1095 Fragments arranged by size: 1095 510 376 163 137 45 26 ApaI G_GGCC'C Cuts at: 0 255 2107 2352 Size: 255 1852 245 Fragments arranged by size: 1852 255 245 ApaLI G'TGCA_C Cuts at: 0 169 782 1450 1606 2035 2352 Size: 169 613 668 156 429 317 Fragments arranged by size: 668 613 429 317 169 156 AvaI C'yCGr_G Cuts at: 0 486 2146 2216 2352 Size: 486 1660 70 136 Fragments arranged by size: 1660 486 136 70 BanI G'GyrC_C Cuts at: 0 138 312 710 794 985 1168 1290 1775 Size: 138 174 398 84 191 183 122 485 Cuts at: 1775 1984 2352 Size: 209 368 Fragments arranged by size: 485 398 368 209 191 183 174 138 122 84 BanII G_rGCy'C Cuts at: 0 255 341 518 700 1058 1076 1681 2107 Size: 255 86 177 182 358 18 605 426 Cuts at: 2107 2352 Size: 245 Fragments arranged by size: 605 426 358 255 245 182 177 86 18 BbeI G_GCGC'C Cuts at: 0 142 2352 Size: 142 2210 BbsI GAAGACnn'nnnn_ Cuts at: 0 1874 2352 Size: 1874 478 BbvCI CC'TCA_GC Cuts at: 0 449 1284 2352 Size: 449 835 1068 Fragments arranged by size: 1068 835 449 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 34 1921 1955 2352 Size: 34 1887 34 397 Fragments arranged by size: 1887 397 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 379 1279 2218 2352 Size: 379 900 939 134 Fragments arranged by size: 939 900 379 134 Bme1580I G_kGCm'C Cuts at: 0 173 255 425 786 988 1173 1295 1454 Size: 173 82 170 361 202 185 122 159 Cuts at: 1454 1610 1987 2039 2107 2203 2352 Size: 156 377 52 68 96 149 Fragments arranged by size: 377 361 202 185 173 170 159 156 149 122 96 82 68 52 BmgBI CAC'GTC Cuts at: 0 852 1008 2352 Size: 852 156 1344 Fragments arranged by size: 1344 852 156 BmtI G_CTAG'C Cuts at: 0 2048 2352 Size: 2048 304 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 390 1695 2352 Size: 390 1305 657 Fragments arranged by size: 1305 657 390 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 908 914 1068 1195 1279 1369 1645 1825 Size: 908 6 154 127 84 90 276 180 Cuts at: 1825 2305 2350 2352 Size: 480 45 2 Fragments arranged by size: 908 480 276 180 154 127 90 84 45 6 2 Bpu10I CC'TnA_GC Cuts at: 0 449 1284 2352 Size: 449 835 1068 Fragments arranged by size: 1068 835 449 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 604 784 1165 1417 2352 Size: 604 180 381 252 935 Fragments arranged by size: 935 604 381 252 180 BsaI GGTCTCn'nnnn_ Cuts at: 0 1318 1948 2352 Size: 1318 630 404 Fragments arranged by size: 1318 630 404 BsaAI yAC'GTr Cuts at: 0 877 1455 1641 1695 2352 Size: 877 578 186 54 657 Fragments arranged by size: 877 657 578 186 54 BsaHI Gr'CG_yC Cuts at: 0 139 2352 Size: 139 2213 BsaWI w'CCGG_w Cuts at: 0 258 2352 Size: 258 2094 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 733 763 1882 1912 2352 Size: 733 30 1119 30 440 Fragments arranged by size: 1119 733 440 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 349 1177 1642 1828 2104 2352 Size: 349 828 465 186 276 248 Fragments arranged by size: 828 465 349 276 248 186 BseYI C'CCAG_C Cuts at: 0 115 297 459 510 1156 1205 2100 2352 Size: 115 182 162 51 646 49 895 252 Fragments arranged by size: 895 646 252 182 162 115 51 49 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 104 801 899 1096 1270 1338 2044 2089 Size: 104 697 98 197 174 68 706 45 Cuts at: 2089 2352 Size: 263 Fragments arranged by size: 706 697 263 197 174 104 98 68 45 BsiEI CG_ry'CG Cuts at: 0 32 183 1743 2352 Size: 32 151 1560 609 Fragments arranged by size: 1560 609 151 32 BsiHKAI G_wGCw'C Cuts at: 0 173 751 786 1076 1454 1610 1760 2039 Size: 173 578 35 290 378 156 150 279 Cuts at: 2039 2352 Size: 313 Fragments arranged by size: 578 378 313 290 279 173 156 150 35 BsmBI CGTCTCn'nnnn_ Cuts at: 0 458 1068 2086 2352 Size: 458 610 1018 266 Fragments arranged by size: 1018 610 458 266 Bsp1286I G_dGCh'C Cuts at: 0 173 255 341 425 518 700 751 786 Size: 173 82 86 84 93 182 51 35 Cuts at: 786 988 1058 1076 1173 1295 1454 1610 1681 Size: 202 70 18 97 122 159 156 71 Cuts at: 1681 1760 1987 2039 2107 2203 2352 Size: 79 227 52 68 96 149 Fragments arranged by size: 227 202 182 173 159 156 149 122 97 96 93 86 84 82 79 71 70 68 52 51 35 18 BspEI T'CCGG_A Cuts at: 0 258 2352 Size: 258 2094 BspHI T'CATG_A Cuts at: 0 2012 2352 Size: 2012 340 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 871 1115 1436 1593 1787 2154 2352 Size: 871 244 321 157 194 367 198 Fragments arranged by size: 871 367 321 244 198 194 157 BsrBI CCG'CTC Cuts at: 0 34 1548 2352 Size: 34 1514 804 Fragments arranged by size: 1514 804 34 BsrDI GCAATG_nn' Cuts at: 0 1505 2135 2352 Size: 1505 630 217 Fragments arranged by size: 1505 630 217 BsrFI r'CCGG_y Cuts at: 0 315 716 901 1236 1526 1772 2050 2352 Size: 315 401 185 335 290 246 278 302 Fragments arranged by size: 401 335 315 302 290 278 246 185 BssHII G'CGCG_C Cuts at: 0 1149 1394 2352 Size: 1149 245 958 Fragments arranged by size: 1149 958 245 BssSI C'ACGA_G Cuts at: 0 658 2352 Size: 658 1694 BstAPI GCAn_nnn'nTGC Cuts at: 0 2034 2352 Size: 2034 318 BstXI CCAn_nnnn'nTGG Cuts at: 0 348 1519 2178 2352 Size: 348 1171 659 174 Fragments arranged by size: 1171 659 348 174 BstYI r'GATC_y Cuts at: 0 2279 2352 Size: 2279 73 BtgI C'CryG_G Cuts at: 0 1659 1743 1841 1901 1967 2081 2255 2352 Size: 1659 84 98 60 66 114 174 97 Fragments arranged by size: 1659 174 114 98 97 84 66 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 25 2292 2352 Size: 25 2267 60 Fragments arranged by size: 2267 60 25 BtsI GCAGTG_nn' Cuts at: 0 23 1223 1326 1405 1937 2352 Size: 23 1200 103 79 532 415 Fragments arranged by size: 1200 532 415 103 79 23 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 654 689 2352 Size: 654 35 1663 Fragments arranged by size: 1663 654 35 DraIII CAC_nnn'GTG Cuts at: 0 360 969 1881 2352 Size: 360 609 912 471 Fragments arranged by size: 912 609 471 360 DrdI GACnn_nn'nnGTC Cuts at: 0 856 2259 2352 Size: 856 1403 93 Fragments arranged by size: 1403 856 93 EaeI y'GGCC_r Cuts at: 0 29 180 476 567 1271 1521 1740 2052 Size: 29 151 296 91 704 250 219 312 Cuts at: 2052 2168 2186 2352 Size: 116 18 166 Fragments arranged by size: 704 312 296 250 219 166 151 116 91 29 18 EagI C'GGCC_G Cuts at: 0 29 180 1740 2352 Size: 29 151 1560 612 Fragments arranged by size: 1560 612 151 29 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 469 2352 Size: 469 1883 EcoICRI GAG'CTC Cuts at: 0 1074 2352 Size: 1074 1278 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 129 819 908 914 1068 1195 1279 1369 Size: 129 690 89 6 154 127 84 90 Cuts at: 1369 1596 1645 1825 2305 2350 2352 Size: 227 49 180 480 45 2 Fragments arranged by size: 690 480 227 180 154 129 127 90 89 84 49 45 6 2 EcoNI CCTnn'n_nnAGG Cuts at: 0 1804 2352 Size: 1804 548 EcoO109I rG'GnC_Cy Cuts at: 0 252 264 483 677 1298 2352 Size: 252 12 219 194 621 1054 Fragments arranged by size: 1054 621 252 219 194 12 FspI TGC'GCA Cuts at: 0 1783 2352 Size: 1783 569 HaeII r_GCGC'y Cuts at: 0 142 738 1467 1581 2051 2101 2352 Size: 142 596 729 114 470 50 251 Fragments arranged by size: 729 596 470 251 142 114 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 18 50 1882 1914 2352 Size: 18 32 1832 32 438 Fragments arranged by size: 1832 438 32 32 18 KasI G'GCGC_C Cuts at: 0 138 2352 Size: 138 2214 MscI TGG'CCA Cuts at: 0 1273 1523 2170 2352 Size: 1273 250 647 182 Fragments arranged by size: 1273 647 250 182 MslI CAynn'nnrTG Cuts at: 0 346 361 728 1457 1517 2017 2352 Size: 346 15 367 729 60 500 335 Fragments arranged by size: 729 500 367 346 335 60 15 MspA1I CmG'CkG Cuts at: 0 119 185 249 1233 1257 1288 1475 1843 Size: 119 66 64 984 24 31 187 368 Cuts at: 1843 2328 2352 Size: 485 24 Fragments arranged by size: 984 485 368 187 119 66 64 31 24 24 NaeI GCC'GGC Cuts at: 0 317 903 2052 2352 Size: 317 586 1149 300 Fragments arranged by size: 1149 586 317 300 NarI GG'CG_CC Cuts at: 0 139 2352 Size: 139 2213 NcoI C'CATG_G Cuts at: 0 2081 2255 2352 Size: 2081 174 97 Fragments arranged by size: 2081 174 97 NgoMIV G'CCGG_C Cuts at: 0 315 901 2050 2352 Size: 315 586 1149 302 Fragments arranged by size: 1149 586 315 302 NheI G'CTAG_C Cuts at: 0 2044 2352 Size: 2044 308 NotI GC'GGCC_GC Cuts at: 0 180 2352 Size: 180 2172 NspI r_CATG'y Cuts at: 0 2293 2352 Size: 2293 59 PasI CC'CwG_GG Cuts at: 0 1029 2352 Size: 1029 1323 PflMI CCAn_nnn'nTGG Cuts at: 0 309 360 1212 2352 Size: 309 51 852 1140 Fragments arranged by size: 1140 852 309 51 PfoI T'CCnGG_A Cuts at: 0 770 2282 2352 Size: 770 1512 70 Fragments arranged by size: 1512 770 70 PmlI CAC'GTG Cuts at: 0 877 1455 1641 1695 2352 Size: 877 578 186 54 657 Fragments arranged by size: 877 657 578 186 54 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1705 1737 2352 Size: 1705 32 615 Fragments arranged by size: 1705 615 32 PpuMI rG'GwC_Cy Cuts at: 0 1298 2352 Size: 1298 1054 PspOMI G'GGCC_C Cuts at: 0 251 2103 2352 Size: 251 1852 249 Fragments arranged by size: 1852 251 249 PstI C_TGCA'G Cuts at: 0 1657 2352 Size: 1657 695 PvuII CAG'CTG Cuts at: 0 1233 2328 2352 Size: 1233 1095 24 Fragments arranged by size: 1233 1095 24 SacI G_AGCT'C Cuts at: 0 1076 2352 Size: 1076 1276 SacII CC_GC'GG Cuts at: 0 1844 2352 Size: 1844 508 SexAI A'CCwGG_T Cuts at: 0 965 2352 Size: 965 1387 SfcI C'TryA_G Cuts at: 0 941 1653 2352 Size: 941 712 699 Fragments arranged by size: 941 712 699 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1279 2352 Size: 1279 1073 SfoI GGC'GCC Cuts at: 0 140 2352 Size: 140 2212 SgrAI Cr'CCGG_yG Cuts at: 0 716 1526 2352 Size: 716 810 826 Fragments arranged by size: 826 810 716 SmaI CCC'GGG Cuts at: 0 488 2218 2352 Size: 488 1730 134 Fragments arranged by size: 1730 488 134 SmlI C'TyrA_G Cuts at: 0 619 799 1144 1432 2352 Size: 619 180 345 288 920 Fragments arranged by size: 920 619 345 288 180 SphI G_CATG'C Cuts at: 0 2293 2352 Size: 2293 59 SrfI GCCC'GGGC Cuts at: 0 2218 2352 Size: 2218 134 StuI AGG'CCT Cuts at: 0 241 1282 1411 1423 2352 Size: 241 1041 129 12 929 Fragments arranged by size: 1041 929 241 129 12 StyI C'CwwG_G Cuts at: 0 107 206 692 980 1418 2081 2255 2352 Size: 107 99 486 288 438 663 174 97 Fragments arranged by size: 663 486 438 288 174 107 99 97 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1319 1930 2002 2352 Size: 1319 611 72 350 Fragments arranged by size: 1319 611 350 72 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 646 678 2352 Size: 646 32 1674 Fragments arranged by size: 1674 646 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 294 2253 2352 Size: 294 1959 99 Fragments arranged by size: 1959 294 99 XmaI C'CCGG_G Cuts at: 0 486 2216 2352 Size: 486 1730 136 Fragments arranged by size: 1730 486 136 XmnI GAAnn'nnTTC Cuts at: 0 1720 2352 Size: 1720 632 Enzymes that do cut: AarI AcuI AfeI AflIII AleI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI Bme1580I BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NotI NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SacII SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SphI SrfI StuI StyI TaqII TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BglII BlpI BmrI BsaBI BsiWI BsmI BsrGI BstBI BstEII BstZ17I Bsu36I ClaI DraI EarI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI NruI NsiI PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SgfI SnaBI SpeI SspI SwaI TatI Tth111I XbaI XhoI ZraI