Restriction map for
pFN21AE9565
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AocI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BamHI BbrPI
BbvCI BciVI BfaI BfrI BlnI
BlpI BpmI Bpu1102I BsaWI BseAI
BseRI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRV
EcoT22I FauI FseI HgaI HindIII
HpaI KpnI KspI MaeI MluI
MroI MseI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccII 919 CGCG CG^CG
AcsI 718 GAATTC [GA]^AATT[CT]
AcyI 891 GGCGCC G[GA]^CG[CT]C
AfaI 1053 GTAC GT^AC
Alw44I 408 GTGCAC G^TGCAC
AlwNI 533 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 726 GGGCCC GGGCC^C
ApaLI 408 GTGCAC G^TGCAC
ApoI 718 GAATTC [GA]^AATT[CT]
AvaI 767 CCCGGG C^[CT]CG[GA]G
BanI 891 GGCGCC G^G[CT][GA]CC
BbeI 891 GGCGCC GGCGC^C
BbsI 840 GAAGAC GAAGAC(2/6)
BcgI 713 GCATAGAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1007 TGATCA T^GATCA
BpuAI 840 GAAGAC GAAGAC(2/6)
BsaAI 877 TACGTG [CT]AC^GT[GA]
BsaBI 576 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 891 GGCGCC G[GA]^CG[CT]C
BsgI 1112 GTGCAG GTGCAG(16/14)
BsmBI 607 GAGACG CGTCTC(1/5)
BsmFI 790 GTCCC GGGAC(10/14)
BsoBI 767 CCCGGG C^[CT]CG[GA]G
BstEII 735 GGTCACC G^GT[ACGT]ACC
BstPI 735 GGTCACC G^GT[ACGT]ACC
BstUI 919 CGCG CG^CG
DdeI 508 CTGAG C^T[ACGT]AG
DraII 726 GGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 1014 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1153 GAAGAG CTCTTC(1/4)
EcoO109I 726 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 735 GGTCACC G^GT[ACGT]ACC
EcoRI 718 GAATTC G^AATTC
FbaI 1007 TGATCA T^GATCA
HaeII 891 GGCGCC [GA]GCGC^[CT]
HapII 768 CCGG C^CGG
Hin1I 891 GGCGCC G[GA]^CG[CT]C
HincII 418 GTCAAC GT[CT]^[GA]AC
HindII 418 GTCAAC GT[CT]^[GA]AC
HinfI 797 GATTC G^A[ACGT]TC
HpaII 768 CCGG C^CGG
KasI 891 GGCGCC G^GCGCC
Ksp632I 1153 GAAGAG CTCTTC(1/4)
MamI 576 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1133 CAATTG C^AATTG
MspA1I 505 CCGCTG C[AC]G^C[GT]G
MspI 768 CCGG C^CGG
MunI 1133 CAATTG C^AATTG
MvnI 919 CGCG CG^CG
NarI 891 GGCGCC GG^CGCC
NcoI 11 CCATGG C^CATGG
RsaI 1053 GTAC GT^AC
SacI 772 GAGCTC GAGCT^C
ScaI 1052 AGTACT AGT^ACT
SfcI 114 CTACAG C^T[GA][CT]AG
SfoI 891 GGCGCC GGC^GCC
SmaI 767 CCCGGG CCC^GGG
SmlI 1126 CTCAAG C^T[CT][GA]AG
TaqI 980 TCGA T^CGA
TfiI 797 GATTC G^A[AT]TC
TthHB8I 980 TCGA T^CGA
XmaCI 767 CCCGGG C^CCGGG
XmaI 767 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwI 813 GATCC GGATC(4/5)
761 GGATC
AspI 561 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1042 GACACCGTC
AviII 278 TGCGCA TGC^GCA
428 TGCGCA
BcnI 767 CCCGG CC^[GC]GG
768 CCGGG
BglI 304 GCCAACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
460 GCCCTGCTGGC
BglII 641 AGATCT A^GATCT
656 AGATCT
BsaI 4 GAGACC GGTCTC(1/5)
510 GAGACC
BspMI 293 ACCTGC ACCTGC(4/8)
425 ACCTGC
EcoT14I 11 CCATGG C^C[AT][AT]GG
551 CCTTGG
FspI 278 TGCGCA TGC^GCA
428 TGCGCA
MwoI 304 GCCAACCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
460 GCCCTGCTGGC
NciI 767 CCCGG CC^[GC]GG
768 CCGGG
PflFI 561 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1042 GACACCGTC
StyI 11 CCATGG C^C[AT][AT]GG
551 CCTTGG
Tth111I 561 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1042 GACACCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AluI AspHI AvaII BalI
BanII BbvI BmyI BsaJI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DpnII DsaI EaeI
EcoRII Fnu4HI FokI HaeIII HhaI
HinP1I HphI ItaI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MvaI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TseI Tsp45I Tsp509I TspRI XcmI
XhoII