(Circular) (Six-base) MAPSORT of: pFN21AE9565.seq Check: 14 from: 1 to: 5884 pFN21AE9565 5884 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3694 278 Size: 53 83 186 936 2158 2468 Fragments arranged by size: 2468 2158 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3227 1937 Size: 1290 4594 Acc65I G'GTAC_C Cuts at: 3205 3205 Size: 5884 AclI AA'CG_TT Cuts at: 1563 4011 4384 1563 Size: 2448 373 3063 Fragments arranged by size: 3063 2448 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2098 3947 5331 5599 1632 Size: 466 1849 1384 268 1917 Fragments arranged by size: 1917 1849 1384 466 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5687 AflIII A'CryG_T Cuts at: 1203 4783 5760 1203 Size: 3580 977 1327 Fragments arranged by size: 3580 1327 977 AgeI A'CCGG_T Cuts at: 4715 4715 Size: 5884 AhdI GACnn_n'nnGTC Cuts at: 4613 4613 Size: 5884 AleI CACnn'nnGTG Cuts at: 1166 2918 1166 Size: 1752 4132 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2846 1184 Size: 1662 4222 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2768 2800 2768 Size: 32 5852 AlwNI CAG_nnn'CTG Cuts at: 2543 3627 5199 2543 Size: 1084 1572 3228 Fragments arranged by size: 3228 1572 1084 ApaI G_GGCC'C Cuts at: 2735 2735 Size: 5884 ApaLI G'TGCA_C Cuts at: 2413 3941 5097 2413 Size: 1528 1156 3200 Fragments arranged by size: 3200 1528 1156 ApoI r'AATT_y Cuts at: 1493 2723 3193 3419 3571 5680 1493 Size: 1230 470 226 152 2109 1697 Fragments arranged by size: 2109 1697 1230 470 226 152 AseI AT'TA_AT Cuts at: 160 1032 4438 160 Size: 872 3406 1606 Fragments arranged by size: 3406 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5884 AvaI C'yCGr_G Cuts at: 1870 1943 2772 3209 1870 Size: 73 829 437 4545 Fragments arranged by size: 4545 829 437 73 BamHI G'GATC_C Cuts at: 3214 3581 3214 Size: 367 5517 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2896 3205 4660 618 Size: 325 217 627 1109 309 1455 1842 Fragments arranged by size: 1842 1455 1109 627 325 309 217 BanII G_rGCy'C Cuts at: 1391 2089 2735 2781 3203 1391 Size: 698 646 46 422 4072 Fragments arranged by size: 4072 698 646 422 46 BbeI G_GCGC'C Cuts at: 2900 2900 Size: 5884 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2852 928 Size: 971 953 3960 Fragments arranged by size: 3960 971 953 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2707 2741 4075 4109 1903 Size: 34 770 34 1334 34 3678 Fragments arranged by size: 3678 1334 770 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3776 4986 3776 Size: 1210 4674 BclI T'GATC_A Cuts at: 1548 3012 1548 Size: 1464 4420 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2315 2471 4495 Size: 107 122 71 631 605 643 156 2024 Cuts at: 4495 136 Size: 1525 Fragments arranged by size: 2024 1525 643 631 605 156 122 107 71 BglII A'GATC_T Cuts at: 2646 2661 5879 2646 Size: 15 3218 2651 Fragments arranged by size: 3218 2651 15 BlpI GC'TnA_GC Cuts at: 3301 3301 Size: 5884 Bme1580I G_kGCm'C Cuts at: 1790 2417 2735 3945 5101 1790 Size: 627 318 1210 1156 2573 Fragments arranged by size: 2573 1210 1156 627 318 BmgBI CAC'GTC Cuts at: 1318 2407 1318 Size: 1089 4795 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3615 4573 442 Size: 416 53 83 399 24 2198 958 1753 Fragments arranged by size: 2198 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5884 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4544 1239 Size: 3305 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3116 4009 4874 5172 3116 Size: 893 865 298 3828 Fragments arranged by size: 3828 893 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2003 2509 4547 882 Size: 617 504 506 2038 2219 Fragments arranged by size: 2219 2038 617 506 504 BsaAI yAC'GTr Cuts at: 493 1204 2884 3675 493 Size: 711 1680 791 2702 Fragments arranged by size: 2702 1680 791 711 BsaBI GATnn'nnATC Cuts at: 2585 5878 2585 Size: 3293 2591 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2897 3691 4073 4708 Size: 53 83 186 936 1364 794 382 635 Cuts at: 4708 275 Size: 1451 Fragments arranged by size: 1451 1364 936 794 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4317 4715 4989 5136 5547 4317 Size: 398 274 147 411 4654 Fragments arranged by size: 4654 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2768 2798 1178 Size: 30 165 30 1365 30 4264 Fragments arranged by size: 4264 1365 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5884 BseYI C'CCAG_C Cuts at: 2892 5087 5583 2892 Size: 2195 496 3193 Fragments arranged by size: 3193 2195 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3138 1163 Size: 573 1402 3909 Fragments arranged by size: 3909 1402 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3334 4095 5123 1540 Size: 275 186 1333 761 1028 2301 Fragments arranged by size: 2301 1333 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2073 2400 2417 2781 2862 3945 4030 5101 2073 Size: 327 17 364 81 1083 85 1071 2856 Fragments arranged by size: 2856 1083 1071 364 327 85 81 17 BsmI GAATG_Cn' Cuts at: 3395 3488 3395 Size: 93 5791 BsmBI CGTCTCn'nnnn_ Cuts at: 2606 2606 Size: 5884 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2089 2400 2417 2735 2781 2862 Size: 399 283 16 311 17 318 46 81 Cuts at: 2862 3203 3945 4030 5101 1391 Size: 341 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 341 318 311 283 85 81 46 17 16 BspEI T'CCGG_A Cuts at: 5547 5547 Size: 5884 BspHI T'CATG_A Cuts at: 3773 5503 3773 Size: 1730 4154 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2307 2439 3239 844 Size: 1463 132 800 3489 Fragments arranged by size: 3489 1463 800 132 BsrBI CCG'CTC Cuts at: 1394 1823 3771 1394 Size: 429 1948 3507 Fragments arranged by size: 3507 1948 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4379 4553 65 Size: 1183 3131 174 1396 Fragments arranged by size: 3131 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4528 4715 1700 Size: 110 2718 187 2869 Fragments arranged by size: 2869 2718 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5884 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5884 BssSI C'ACGA_G Cuts at: 3944 4956 3944 Size: 1012 4872 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5884 BstBI TT'CG_AA Cuts at: 4688 4688 Size: 5884 BstEII G'GTnAC_C Cuts at: 1184 2740 1184 Size: 1556 4328 BstYI r'GATC_y Cuts at: 1972 2646 2661 2766 2817 3214 3581 3966 3983 Size: 674 15 105 51 397 367 385 17 Cuts at: 3983 5435 5521 5533 5879 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 674 397 385 367 346 105 86 51 17 15 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 2519 2736 513 Size: 552 939 12 503 217 3661 Fragments arranged by size: 3661 939 552 503 217 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2290 2461 2977 487 Size: 683 56 1064 171 516 3394 Fragments arranged by size: 3394 1064 683 516 171 56 BtsI GCAGTG_nn' Cuts at: 2967 3400 4194 4214 2967 Size: 433 794 20 4637 Fragments arranged by size: 4637 794 433 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5884 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5849 DraI TTT'AAA Cuts at: 3188 3188 Size: 5884 DraIII CAC_nnn'GTG Cuts at: 3024 3024 Size: 5884 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4891 809 Size: 507 3575 1802 Fragments arranged by size: 3575 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2316 2337 2401 Size: 54 1425 50 275 18 486 21 64 Cuts at: 2401 2472 2656 3331 4220 5849 8 Size: 71 184 675 889 1629 43 Fragments arranged by size: 1629 1425 889 675 486 275 184 71 64 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 3331 1537 Size: 275 1519 4090 Fragments arranged by size: 4090 1519 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3153 3814 1353 Size: 1800 661 3423 Fragments arranged by size: 3423 1800 661 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2584 4471 4845 4991 387 Size: 1064 138 995 1887 374 146 1280 Fragments arranged by size: 1887 1280 1064 995 374 146 138 EcoICRI GAG'CTC Cuts at: 2779 2779 Size: 5884 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2098 3947 4544 5331 5599 1239 Size: 393 466 1849 597 787 268 1524 Fragments arranged by size: 1849 1524 787 597 466 393 268 EcoO109I rG'GnC_Cy Cuts at: 2732 2732 Size: 5884 EcoRI G'AATT_C Cuts at: 2723 3193 3571 2723 Size: 470 378 5036 Fragments arranged by size: 5036 470 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2716 2716 Size: 5884 FspI TGC'GCA Cuts at: 2285 2435 4390 2285 Size: 150 1955 3779 Fragments arranged by size: 3779 1955 150 HaeII r_GCGC'y Cuts at: 1678 2900 5031 1678 Size: 1222 2131 2531 Fragments arranged by size: 2531 2131 1222 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3004 3036 4525 4557 4599 Size: 32 135 32 1432 32 1489 32 42 Cuts at: 4599 4631 1373 Size: 32 2626 Fragments arranged by size: 2626 1489 1432 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2425 3228 3474 669 Size: 988 281 487 803 246 3079 Fragments arranged by size: 3079 988 803 487 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5884 HpaI GTT'AAC Cuts at: 3474 3474 Size: 5884 KasI G'GCGC_C Cuts at: 2896 2896 Size: 5884 KpnI G_GTAC'C Cuts at: 3209 3209 Size: 5884 MfeI C'AATT_G Cuts at: 3138 3483 3138 Size: 345 5539 MluI A'CGCG_T Cuts at: 5760 5760 Size: 5884 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4998 5182 1305 Size: 3693 184 2007 Fragments arranged by size: 3693 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2318 2339 2474 10 Size: 54 1425 343 486 21 135 3420 Fragments arranged by size: 3420 1425 486 343 135 54 21 MslI CAynn'nnrTG Cuts at: 518 1166 2201 2918 2996 3842 4201 4360 518 Size: 648 1035 717 78 846 359 159 2042 Fragments arranged by size: 2042 1035 846 717 648 359 159 78 MspA1I CmG'CkG Cuts at: 1606 2512 3300 3977 5125 5370 1606 Size: 906 788 677 1148 245 2120 Fragments arranged by size: 2120 1148 906 788 677 245 NarI GG'CG_CC Cuts at: 2897 2897 Size: 5884 NcoI C'CATG_G Cuts at: 513 1065 2004 2016 513 Size: 552 939 12 4381 Fragments arranged by size: 4381 939 552 12 NdeI CA'TA_TG Cuts at: 387 387 Size: 5884 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5884 NotI GC'GGCC_GC Cuts at: 3331 3331 Size: 5884 NspI r_CATG'y Cuts at: 1132 1229 3242 4787 1132 Size: 97 2013 1545 2229 Fragments arranged by size: 2229 2013 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5884 PciI A'CATG_T Cuts at: 4783 4783 Size: 5884 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5884 PfoI T'CCnGG_A Cuts at: 2168 2168 Size: 5884 PmeI GTTT'AAAC Cuts at: 3188 3188 Size: 5884 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2768 2800 3953 3985 5466 5498 2768 Size: 32 1153 32 1481 32 3154 Fragments arranged by size: 3154 1481 1153 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5884 PsiI TTA'TAA Cuts at: 3454 3454 Size: 5884 PspOMI G'GGCC_C Cuts at: 2731 2731 Size: 5884 PstI C_TGCA'G Cuts at: 830 3236 830 Size: 2406 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5884 SacI G_AGCT'C Cuts at: 2781 2781 Size: 5884 SalI G'TCGA_C Cuts at: 1936 3226 1936 Size: 1290 4594 SbfI CC_TGCA'GG Cuts at: 3236 3236 Size: 5884 ScaI AGT'ACT Cuts at: 3059 4132 5844 3059 Size: 1073 1712 3099 Fragments arranged by size: 3099 1712 1073 SfcI C'TryA_G Cuts at: 826 1045 2119 3232 4367 5048 5239 826 Size: 219 1074 1113 1135 681 191 1471 Fragments arranged by size: 1471 1135 1113 1074 681 219 191 SfoI GGC'GCC Cuts at: 2898 2898 Size: 5884 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5884 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5884 SmaI CCC'GGG Cuts at: 1872 2774 3211 1872 Size: 902 437 4545 Fragments arranged by size: 4545 902 437 SmlI C'TyrA_G Cuts at: 820 1017 1943 3131 3988 4889 5151 820 Size: 197 926 1188 857 901 262 1553 Fragments arranged by size: 1553 1188 926 901 857 262 197 SnaBI TAC'GTA Cuts at: 493 3675 493 Size: 3182 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5884 SphI G_CATG'C Cuts at: 1132 3242 1132 Size: 2110 3774 SspI AAT'ATT Cuts at: 5 52 3808 5 Size: 47 3756 2081 Fragments arranged by size: 3756 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5884 StyI C'CwwG_G Cuts at: 513 1065 2004 2016 2556 3323 513 Size: 552 939 12 540 767 3074 Fragments arranged by size: 3074 939 767 552 540 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3911 3928 4081 4266 4711 325 Size: 1255 2331 17 153 185 445 1498 Fragments arranged by size: 2331 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3057 4130 5842 Size: 274 80 33 51 1443 1080 1073 1712 Cuts at: 5842 96 Size: 138 Fragments arranged by size: 1712 1443 1080 1073 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5844 5876 5844 Size: 32 5852 Tth111I GACn'n_nGTC Cuts at: 1316 2569 3050 1316 Size: 1253 481 4150 Fragments arranged by size: 4150 1253 481 XbaI T'CTAG_A Cuts at: 3220 3220 Size: 5884 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2023 2467 2540 3121 3160 2023 Size: 444 73 581 39 4747 Fragments arranged by size: 4747 581 444 73 39 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5884 XmaI C'CCGG_G Cuts at: 1870 2772 3209 1870 Size: 902 437 4545 Fragments arranged by size: 4545 902 437 XmnI GAAnn'nnTTC Cuts at: 4013 4013 Size: 5884 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3692 276 Size: 53 83 186 936 2158 2468 Fragments arranged by size: 2468 2158 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI