################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbrPI BcgI BciVI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrFI BssHII Bst1107I BstBI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI MamI MfeI MluI MroI MseI MslI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 248 GGTACC G^GTACC Asp718I 248 GGTACC G^GTACC AspHI 1111 GAGCAC G[AT]GC[AT]^C BamHI 914 GGATCC G^GATCC BbeI 1253 GGCGCC GGCGC^C BbsI 931 GTCTTC GAAGAC(2/6) BclI 947 TGATCA T^GATCA BfaI 1409 CTAG C^TAG BpuAI 931 GTCTTC GAAGAC(2/6) BsaI 1384 GAGACC GGTCTC(1/5) BsiHKAI 1111 GAGCAC G[AT]GC[AT]^C BsmAI 1384 GAGAC GTCTC(1/5) BssSI 985 CTCGTG CACGAG(-5/-1) BstAPI 747 GCACAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 118 GGTGACC G^GT[ACGT]ACC BstPI 118 GGTGACC G^GT[ACGT]ACC BstYI 914 GGATCC [GA]^GATC[CT] DrdI 625 GACCTCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1206 CCTCAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 118 GGTGACC G^GT[ACGT]ACC FbaI 947 TGATCA T^GATCA HaeII 1253 GGCGCC [GA]GCGC^[CT] KasI 1253 GGCGCC G^GCGCC KpnI 248 GGTACC GGTAC^C KspI 680 CCGCGG CCGC^GG MaeI 1409 CTAG C^TAG MaeII 872 ACGT A^CGT MflI 914 GGATCC [GA]^GATC[CT] NarI 1253 GGCGCC GG^CGCC PflMI 1080 CCACGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 121 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1342 CTGCAG CTGCA^G PvuII 979 CAGCTG CAG^CTG SacII 680 CCGCGG CCGC^GG SapI 1143 GCTCTTC GCTCTTC(1/4) SfoI 1253 GGCGCC GGC^GCC SmaI 329 CCCGGG CCC^GGG Tsp509I 615 AATT ^AATT Van91I 1080 CCACGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 563 CCAGCTCCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 914 GGATCC [GA]^GATC[CT] XmaCI 329 CCCGGG C^CCGGG XmaI 329 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 531 CGCG CG^CG 681 CGCG AcyI 1075 GGCGTC G[GA]^CG[CT]C 1253 GGCGCC AlwI 915 GATCC GGATC(4/5) 914 GGATC AlwNI 636 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG 1174 CAGGGCCTG ApaI 301 GGGCCC GGGCC^C 461 GGGCCC AvaI 329 CCCGGG C^[CT]CG[GA]G 775 CCCGAG BbvCI 495 CCTCAGC CCTCAGC(-5/-2) 1161 CCTCAGC BsaHI 1075 GGCGTC G[GA]^CG[CT]C 1253 GGCGCC BsoBI 329 CCCGGG C^[CT]CG[GA]G 775 CCCGAG Bsp1407I 410 TGTACA T^GTACA 431 TGTACA BsrDI 518 GCAATG GCAATG(2/0) 890 GCAATG BsrGI 410 TGTACA T^GTACA 431 TGTACA BstUI 531 CGCG CG^CG 681 CGCG CfoI 1131 GCGC GCG^C 1254 GCGC DpnII 915 GATC ^GATC 948 GATC DraIII 265 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG 1081 CACGAAGTG EarI 1144 CTCTTC CTCTTC(1/4) 1156 CTCTTC HgaI 544 GCGTC GACGC(5/10) 1076 GCGTC HhaI 1131 GCGC GCG^C 1254 GCGC Hin1I 1075 GGCGTC G[GA]^CG[CT]C 1253 GGCGCC HinP1I 1131 GCGC G^CGC 1254 GCGC HinfI 534 GAATC G^A[ACGT]TC 1372 GATTC Ksp632I 1144 CTCTTC CTCTTC(1/4) 1156 CTCTTC MboI 915 GATC ^GATC 948 GATC MvnI 531 CGCG CG^CG 681 CGCG NdeII 915 GATC ^GATC 948 GATC PpuMI 65 AGGTCCC [GA]G^G[AT]CC[CT] 595 AGGACCC Sau3AI 915 GATC ^GATC 948 GATC SspBI 410 TGTACA T^GTACA 431 TGTACA TfiI 534 GAATC G^A[AT]TC 1372 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AfaI AluI AvaII BalI BanI BanII BbvI BcnI BmyI BsaJI BseRI BsgI BsiYI BslI BsmFI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeIII MboII MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I