################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI ApaLI ApoI AscI Asp700I AspEI AspI AviII AvrII BamHI BbsI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspLU11I Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI Ksp632I MamI MfeI MluI MroI MunI NaeI NcoI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmeI PshAI Psp1406I PvuI RsrII SacI SalI SapI SbfI SexAI SfaNI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 715 GTCTAC GT^[AC][GT]AC AflIII 692 ACACGT A^C[GA][CT]GT Aor51HI 110 AGCGCT AGC^GCT AseI 925 ATTAAT AT^TAAT AsnI 925 ATTAAT AT^TAAT AspHI 736 GTGCTC G[AT]GC[AT]^C BalI 318 TGGCCA TGG^CCA BbeI 1099 GGCGCC GGCGC^C BbrPI 693 CACGTG CAC^GTG BglII 1130 AGATCT A^GATCT BpmI 58 CTCCAG CTGGAG(16/14) BsaAI 693 CACGTG [CT]AC^GT[GA] BsiHKAI 736 GTGCTC G[AT]GC[AT]^C Bsp1407I 419 TGTACA T^GTACA BspHI 911 TCATGA T^CATGA BsrBI 403 GAGCGG CCGCTC(-3/-3) BsrDI 496 CATTGC GCAATG(2/0) BsrFI 1004 ACCGGC [GA]^CCGG[CT] BsrGI 419 TGTACA T^GTACA BssHII 1275 GCGCGC G^CGCGC BstF5I 906 CATCC GGATG(2/0) Cfr10I 1004 ACCGGC [GA]^CCGG[CT] Eco47III 110 AGCGCT AGC^GCT EcoNI 1245 CCTGGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FokI 906 CATCC GGATG(9/13) HphI 822 GGTGA GGTGA(8/7) KasI 1099 GGCGCC G^GCGCC MluNI 318 TGGCCA TGG^CCA MscI 318 TGGCCA TGG^CCA MseI 926 TTAA T^TAA NarI 1099 GGCGCC GG^CGCC NheI 459 GCTAGC G^CTAGC PmaCI 693 CACGTG CAC^GTG PmlI 693 CACGTG CAC^GTG PshBI 925 ATTAAT AT^TAAT PvuII 22 CAGCTG CAG^CTG RcaI 911 TCATGA T^CATGA ScaI 671 AGTACT AGT^ACT SfoI 1099 GGCGCC GGC^GCC SmlI 474 CTTGAG C^T[CT][GA]AG SspBI 419 TGTACA T^GTACA Tru9I 926 TTAA T^TAA Tsp509I 928 AATT ^AATT ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AcyI 712 GGCGTC G[GA]^CG[CT]C 1099 GGCGCC AlwI 15 GATCC GGATC(4/5) 1206 GATCC AlwNI 22 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 1355 CAGGGCCTG ApaI 794 GGGCCC GGGCC^C 1286 GGGCCC BanII 794 GGGCCC G[GA]GC[CT]^C 1286 GGGCCC BfaI 460 CTAG C^TAG 503 CTAG BglI 302 GCCAGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1279 GCCTTCCGGGC BsaHI 712 GGCGTC G[GA]^CG[CT]C 1099 GGCGCC BsmI 947 GCATTC GAATGC(1/-1) 956 GCATTC BsrI 303 CCAGT ACTGG(1/-1) 669 CCAGT BssSI 1057 CACGAG CACGAG(-5/-1) 739 CTCGTG BstYI 1130 AGATCT [GA]^GATC[CT] 1205 AGATCC EaeI 318 TGGCCA [CT]^GGCC[GA] 554 CGGCCA EcoT14I 146 CCTTGG C^C[AT][AT]GG 600 CCTTGG HaeII 110 AGCGCT [GA]GCGC^[CT] 1099 GGCGCC Hin1I 712 GGCGTC G[GA]^CG[CT]C 1099 GGCGCC KspI 155 CCGCGG CCGC^GG 1329 CCGCGG MaeI 460 CTAG C^TAG 503 CTAG MboII 366 GAAGA GAAGA(8/7) 1133 TCTTC MflI 1130 AGATCT [GA]^GATC[CT] 1205 AGATCC MslI 271 CACCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 906 CATCCTCATG PstI 19 CTGCAG CTGCA^G 284 CTGCAG SacII 155 CCGCGG CCGC^GG 1329 CCGCGG SfcI 19 CTGCAG C^T[GA][CT]AG 284 CTGCAG SmaI 1094 CCCGGG CCC^GGG 1308 CCCGGG StuI 171 AGGCCT AGG^CCT 374 AGGCCT StyI 146 CCTTGG C^C[AT][AT]GG 600 CCTTGG TaqI 194 TCGA T^CGA 585 TCGA Tsp45I 664 GTGAC ^GT[GC]AC 1078 GTGAC TthHB8I 194 TCGA T^CGA 585 TCGA XcmI 1025 CCACGCTGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1373 CCAGCCGGGAGTTGG XhoII 1130 AGATCT [GA]^GATC[CT] 1205 AGATCC XmaCI 1094 CCCGGG C^CCGGG 1308 CCCGGG XmaI 1094 CCCGGG C^CCGGG 1308 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### Acc65I AccII AciI AfaI AluI Asp718I AvaI AvaII BanI BbvCI BbvI BcnI BmyI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BssKI BstAPI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HgaI HhaI HinP1I HpaII ItaI KpnI MaeII MaeIII MboI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PpuMI RsaI Sau3AI Sau96I ScrFI TseI TspRI Van91I