(Circular) (Six-base) MAPSORT of: pFN21AE9544.seq Check: 2641 from: 1 to: 6103 pFN21AE9544 6103 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3913 278 Size: 53 83 186 936 2377 2468 Fragments arranged by size: 2468 2377 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2721 3446 1937 Size: 784 725 4594 Fragments arranged by size: 4594 784 725 Acc65I G'GTAC_C Cuts at: 2127 2262 2838 3424 2127 Size: 135 576 586 4806 Fragments arranged by size: 4806 586 576 135 AclI AA'CG_TT Cuts at: 1563 4230 4603 1563 Size: 2667 373 3063 Fragments arranged by size: 3063 2667 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2157 4166 5550 5818 1632 Size: 525 2009 1384 268 1917 Fragments arranged by size: 2009 1917 1384 525 268 AfeI AGC'GCT Cuts at: 2117 2117 Size: 6103 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5906 AflIII A'CryG_T Cuts at: 1203 2697 5002 5979 1203 Size: 1494 2305 977 1327 Fragments arranged by size: 2305 1494 1327 977 AgeI A'CCGG_T Cuts at: 4934 4934 Size: 6103 AhdI GACnn_n'nnGTC Cuts at: 4832 4832 Size: 6103 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6103 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6103 AlwNI CAG_nnn'CTG Cuts at: 2032 3365 3846 5418 2032 Size: 1333 481 1572 2717 Fragments arranged by size: 2717 1572 1333 481 ApaI G_GGCC'C Cuts at: 2803 3295 2803 Size: 492 5611 ApaLI G'TGCA_C Cuts at: 4160 5316 4160 Size: 1156 4947 ApoI r'AATT_y Cuts at: 1493 3412 3638 3790 5899 1493 Size: 1919 226 152 2109 1697 Fragments arranged by size: 2109 1919 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2931 4657 160 Size: 872 1899 1726 1606 Fragments arranged by size: 1899 1726 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 AvaI C'yCGr_G Cuts at: 1870 1943 3099 3313 3346 3428 1870 Size: 73 1156 214 33 82 4545 Fragments arranged by size: 4545 1156 214 82 73 33 BamHI G'GATC_C Cuts at: 3433 3800 3433 Size: 367 5736 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2127 2262 2274 2687 2838 Size: 325 217 627 340 135 12 413 151 Cuts at: 2838 3043 3104 3225 3424 4879 618 Size: 205 61 121 199 1455 1842 Fragments arranged by size: 1842 1455 627 413 340 325 217 205 199 151 135 121 61 12 BanII G_rGCy'C Cuts at: 1391 2803 3295 3422 1391 Size: 1412 492 127 4072 Fragments arranged by size: 4072 1412 492 127 BbeI G_GCGC'C Cuts at: 3108 3108 Size: 6103 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5132 BbvCI CC'TCA_GC Cuts at: 2978 3014 3143 2978 Size: 36 129 5938 Fragments arranged by size: 5938 129 36 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4294 4328 1903 Size: 34 2357 34 3678 Fragments arranged by size: 3678 2357 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3995 5205 3995 Size: 1210 4893 BclI T'GATC_A Cuts at: 1548 1548 Size: 6103 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2313 3290 4714 Size: 107 122 71 631 605 641 977 1424 Cuts at: 4714 136 Size: 1525 Fragments arranged by size: 1525 1424 977 641 631 605 122 107 71 BglII A'GATC_T Cuts at: 3135 6098 3135 Size: 2963 3140 BlpI GC'TnA_GC Cuts at: 3520 3520 Size: 6103 Bme1580I G_kGCm'C Cuts at: 1790 2803 3048 3295 4164 5320 1790 Size: 1013 245 247 869 1156 2573 Fragments arranged by size: 2573 1156 1013 869 247 245 BmgBI CAC'GTC Cuts at: 1318 2538 1318 Size: 1220 4883 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2668 3834 4792 Size: 416 53 83 399 24 1251 1166 958 Cuts at: 4792 442 Size: 1753 Fragments arranged by size: 1753 1251 1166 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2468 1056 Size: 1412 4691 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2098 3039 2098 Size: 941 5162 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2048 4763 1239 Size: 809 2715 2579 Fragments arranged by size: 2715 2579 809 Bpu10I CC'TnA_GC Cuts at: 2978 3014 3143 2978 Size: 36 129 5938 Fragments arranged by size: 5938 129 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2500 4228 5093 5391 2500 Size: 1728 865 298 3212 Fragments arranged by size: 3212 1728 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2141 2337 2568 4766 882 Size: 617 642 196 231 2198 2219 Fragments arranged by size: 2219 2198 642 617 231 196 BsaAI yAC'GTr Cuts at: 493 1204 2700 3894 493 Size: 711 1496 1194 2702 Fragments arranged by size: 2702 1496 1194 711 BsaBI GATnn'nnATC Cuts at: 6097 6097 Size: 6103 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2718 3105 3910 4292 Size: 53 83 186 936 1185 387 805 382 Cuts at: 4292 4927 275 Size: 635 1451 Fragments arranged by size: 1451 1185 936 805 635 387 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4536 4934 5208 5355 5766 4536 Size: 398 274 147 411 4873 Fragments arranged by size: 4873 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1999 2029 3188 3218 3255 Size: 30 165 30 596 30 1159 30 37 Cuts at: 3255 3285 1178 Size: 30 3996 Fragments arranged by size: 3996 1159 596 165 37 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2774 2777 3272 1186 Size: 1588 3 495 4017 Fragments arranged by size: 4017 1588 495 3 BseYI C'CCAG_C Cuts at: 2118 2214 2793 2862 3052 5306 5802 2118 Size: 96 579 69 190 2254 496 2419 Fragments arranged by size: 2419 2254 579 496 190 96 69 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2211 2711 3221 1163 Size: 573 475 500 510 4045 Fragments arranged by size: 4045 573 510 500 475 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3553 4314 5342 1540 Size: 275 186 1552 761 1028 2301 Fragments arranged by size: 2301 1552 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2745 4164 4249 5320 2745 Size: 1419 85 1071 3528 Fragments arranged by size: 3528 1419 1071 85 BsmI GAATG_Cn' Cuts at: 2952 2961 3614 3707 2952 Size: 9 653 93 5348 Fragments arranged by size: 5348 653 93 9 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2745 2803 3048 3295 3422 4164 4249 Size: 399 955 58 245 247 127 742 85 Cuts at: 4249 5320 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 955 742 399 247 245 127 85 58 BspEI T'CCGG_A Cuts at: 5766 5766 Size: 6103 BspHI T'CATG_A Cuts at: 2916 3992 5722 2916 Size: 1076 1730 3297 Fragments arranged by size: 3297 1730 1076 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2274 2282 2407 2733 2790 3458 844 Size: 1430 8 125 326 57 668 3489 Fragments arranged by size: 3489 1430 668 326 125 57 8 BsrBI CCG'CTC Cuts at: 1394 1823 2410 3990 1394 Size: 429 587 1580 3507 Fragments arranged by size: 3507 1580 587 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2500 4598 4772 65 Size: 1183 1252 2098 174 1396 Fragments arranged by size: 2098 1396 1252 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3009 4747 4934 1700 Size: 110 1199 1738 187 2869 Fragments arranged by size: 2869 1738 1199 187 110 BsrGI T'GTAC_A Cuts at: 96 2424 96 Size: 2328 3775 BssHII G'CGCG_C Cuts at: 1925 3280 1925 Size: 1355 4748 BssSI C'ACGA_G Cuts at: 2744 3062 4163 5175 2744 Size: 318 1101 1012 3672 Fragments arranged by size: 3672 1101 1012 318 BstAPI GCAn_nnn'nTGC Cuts at: 794 2032 2139 2234 794 Size: 1238 107 95 4663 Fragments arranged by size: 4663 1238 107 95 BstBI TT'CG_AA Cuts at: 4907 4907 Size: 6103 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6103 BstYI r'GATC_y Cuts at: 1972 3135 3210 3433 3800 4185 4202 5654 5740 Size: 1163 75 223 367 385 17 1452 86 Cuts at: 5740 5752 6098 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1163 385 367 346 223 86 75 17 12 BtgI C'CryG_G Cuts at: 513 1065 2160 3217 3334 513 Size: 552 1095 1057 117 3282 Fragments arranged by size: 3282 1095 1057 552 117 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5364 Fragments arranged by size: 5364 683 56 BtsI GCAGTG_nn' Cuts at: 2238 2323 2456 2864 3229 3619 4413 4433 2238 Size: 85 133 408 365 390 794 20 3908 Fragments arranged by size: 3908 794 408 390 365 133 85 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6103 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6068 DraI TTT'AAA Cuts at: 3407 3407 Size: 6103 DraIII CAC_nnn'GTG Cuts at: 2284 2357 2859 3067 2284 Size: 73 502 208 5320 Fragments arranged by size: 5320 502 208 73 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5110 809 Size: 507 3794 1802 Fragments arranged by size: 3794 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2323 2559 3550 Size: 54 1425 50 275 18 493 236 991 Cuts at: 3550 4439 6068 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 991 889 493 275 236 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3550 1537 Size: 275 1738 4090 Fragments arranged by size: 4090 1738 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4033 1353 Size: 2680 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2087 2294 3203 4690 5064 5210 Size: 1064 138 498 207 909 1487 374 146 Cuts at: 5210 387 Size: 1280 Fragments arranged by size: 1487 1280 1064 909 498 374 207 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2157 4166 4763 5550 5818 1239 Size: 393 416 109 2009 597 787 268 1524 Fragments arranged by size: 2009 1524 787 597 416 393 268 109 EcoNI CCTnn'n_nnAGG Cuts at: 3254 3254 Size: 6103 EcoO109I rG'GnC_Cy Cuts at: 2193 2617 2638 2799 2800 3292 3322 3351 3362 Size: 424 21 161 1 492 30 29 11 Cuts at: 3362 2193 Size: 4934 Fragments arranged by size: 4934 492 424 161 30 29 21 11 1 EcoRI G'AATT_C Cuts at: 3412 3790 3412 Size: 378 5725 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3145 3145 Size: 6103 FspI TGC'GCA Cuts at: 4609 4609 Size: 6103 HaeII r_GCGC'y Cuts at: 1678 2119 3108 5250 1678 Size: 441 989 2142 2531 Fragments arranged by size: 2531 2142 989 441 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2031 3188 3220 4744 Size: 32 135 32 427 32 1157 32 1524 Cuts at: 4744 4776 4818 4850 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1524 1157 427 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3447 3693 669 Size: 988 281 1509 246 3079 Fragments arranged by size: 3079 1509 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6103 HpaI GTT'AAC Cuts at: 3693 3693 Size: 6103 KasI G'GCGC_C Cuts at: 3104 3104 Size: 6103 KpnI G_GTAC'C Cuts at: 2131 2266 2842 3428 2131 Size: 135 576 586 4806 Fragments arranged by size: 4806 586 576 135 MfeI C'AATT_G Cuts at: 3702 3702 Size: 6103 MluI A'CGCG_T Cuts at: 5979 5979 Size: 6103 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3186 3369 5217 5401 1305 Size: 1881 183 1848 184 2007 Fragments arranged by size: 2007 1881 1848 184 183 MscI TGG'CCA Cuts at: 10 64 1489 1832 2325 10 Size: 54 1425 343 493 3788 Fragments arranged by size: 3788 1425 493 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2280 2915 4061 4420 4579 518 Size: 648 1114 635 1146 359 159 2042 Fragments arranged by size: 2042 1146 1114 648 635 359 159 MspA1I CmG'CkG Cuts at: 1606 2029 2162 3336 3519 4196 5344 5589 1606 Size: 423 133 1174 183 677 1148 245 2120 Fragments arranged by size: 2120 1174 1148 677 423 245 183 133 NarI GG'CG_CC Cuts at: 3105 3105 Size: 6103 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5551 NdeI CA'TA_TG Cuts at: 387 387 Size: 6103 NheI G'CTAG_C Cuts at: 1052 2464 1052 Size: 1412 4691 NotI GC'GGCC_GC Cuts at: 3550 3550 Size: 6103 NspI r_CATG'y Cuts at: 1132 1229 3461 5006 1132 Size: 97 2232 1545 2229 Fragments arranged by size: 2232 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2823 3359 1348 Size: 1475 536 4092 Fragments arranged by size: 4092 1475 536 PciI A'CATG_T Cuts at: 5002 5002 Size: 6103 PflMI CCAn_nnn'nTGG Cuts at: 1108 2284 2892 3067 3365 1108 Size: 1176 608 175 298 3846 Fragments arranged by size: 3846 1176 608 298 175 PfoI T'CCnGG_A Cuts at: 3273 3273 Size: 6103 PmeI GTTT'AAAC Cuts at: 3407 3407 Size: 6103 PmlI CAC'GTG Cuts at: 2700 2700 Size: 6103 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4172 4204 5685 5717 4172 Size: 32 1481 32 4558 Fragments arranged by size: 4558 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2193 3322 3351 2193 Size: 1129 29 4945 Fragments arranged by size: 4945 1129 29 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6103 PsiI TTA'TAA Cuts at: 3673 3673 Size: 6103 PspOMI G'GGCC_C Cuts at: 2799 3291 2799 Size: 492 5611 PstI C_TGCA'G Cuts at: 830 2028 2293 3455 830 Size: 1198 265 1162 3478 Fragments arranged by size: 3478 1198 1162 265 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6103 PvuII CAG'CTG Cuts at: 2029 2029 Size: 6103 SacII CC_GC'GG Cuts at: 2163 3337 2163 Size: 1174 4929 SalI G'TCGA_C Cuts at: 1936 3445 1936 Size: 1509 4594 SanDI GG'GwC_CC Cuts at: 3351 3351 Size: 6103 SbfI CC_TGCA'GG Cuts at: 3455 3455 Size: 6103 ScaI AGT'ACT Cuts at: 2678 4351 6063 2678 Size: 1673 1712 2718 Fragments arranged by size: 2718 1712 1673 SfcI C'TryA_G Cuts at: 826 1045 2024 2289 3451 4586 5267 5458 826 Size: 219 979 265 1162 1135 681 191 1471 Fragments arranged by size: 1471 1162 1135 979 681 265 219 191 SfoI GGC'GCC Cuts at: 3106 3106 Size: 6103 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6103 SmaI CCC'GGG Cuts at: 1872 3101 3315 3430 1872 Size: 1229 214 115 4545 Fragments arranged by size: 4545 1229 214 115 SmlI C'TyrA_G Cuts at: 820 1017 1943 2479 4207 5108 5370 820 Size: 197 926 536 1728 901 262 1553 Fragments arranged by size: 1728 1553 926 901 536 262 197 SnaBI TAC'GTA Cuts at: 493 3894 493 Size: 3401 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6103 SphI G_CATG'C Cuts at: 1132 3461 1132 Size: 2329 3774 SrfI GCCC'GGGC Cuts at: 3315 3315 Size: 6103 SspI AAT'ATT Cuts at: 5 52 4027 5 Size: 47 3975 2081 Fragments arranged by size: 3975 2081 47 StuI AGG'CCT Cuts at: 1517 2178 2381 1517 Size: 661 203 5239 Fragments arranged by size: 5239 661 203 StyI C'CwwG_G Cuts at: 513 1065 2151 2605 3542 513 Size: 552 1086 454 937 3074 Fragments arranged by size: 3074 1086 937 552 454 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2245 2675 2815 3044 4130 4147 4300 Size: 1255 665 430 140 229 1086 17 153 Cuts at: 4300 4485 4930 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1086 665 445 430 229 185 153 140 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2424 2676 4349 Size: 274 80 33 51 1443 447 252 1673 Cuts at: 4349 6061 96 Size: 1712 138 Fragments arranged by size: 1712 1673 1443 447 274 252 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6063 6095 6063 Size: 32 6071 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6103 XbaI T'CTAG_A Cuts at: 3439 3439 Size: 6103 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3037 3385 3037 Size: 348 5755 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6103 XmaI C'CCGG_G Cuts at: 1870 3099 3313 3428 1870 Size: 1229 214 115 4545 Fragments arranged by size: 4545 1229 214 115 XmnI GAAnn'nnTTC Cuts at: 4232 4232 Size: 6103 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3911 276 Size: 53 83 186 936 2377 2468 Fragments arranged by size: 2468 2377 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SapI SexAI SfiI SwaI