(Linear) (Six-base) MAPSORT of: KIEE9544.seq Check: 3993 from: 1 to: 1398 KIEE9544.orf With 182 enzymes: SgfI * July 3, 2012 19:02 .. AccI GT'mk_AC Cuts at: 0 716 1398 Size: 716 682 Acc65I G'GTAC_C Cuts at: 0 122 257 833 1398 Size: 122 135 576 565 Fragments arranged by size: 576 565 135 122 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 152 1398 Size: 152 1246 AfeI AGC'GCT Cuts at: 0 112 1398 Size: 112 1286 AflIII A'CryG_T Cuts at: 0 692 1398 Size: 692 706 AlwNI CAG_nnn'CTG Cuts at: 0 27 1360 1398 Size: 27 1333 38 Fragments arranged by size: 1333 38 27 ApaI G_GGCC'C Cuts at: 0 798 1290 1398 Size: 798 492 108 Fragments arranged by size: 798 492 108 AseI AT'TA_AT Cuts at: 0 926 1398 Size: 926 472 AvaI C'yCGr_G Cuts at: 0 1094 1308 1341 1398 Size: 1094 214 33 57 Fragments arranged by size: 1094 214 57 33 BanI G'GyrC_C Cuts at: 0 122 257 269 682 833 1038 1099 1220 Size: 122 135 12 413 151 205 61 121 Cuts at: 1220 1398 Size: 178 Fragments arranged by size: 413 205 178 151 135 122 121 61 12 BanII G_rGCy'C Cuts at: 0 798 1290 1398 Size: 798 492 108 Fragments arranged by size: 798 492 108 BbeI G_GCGC'C Cuts at: 0 1103 1398 Size: 1103 295 BbvCI CC'TCA_GC Cuts at: 0 973 1009 1138 1398 Size: 973 36 129 260 Fragments arranged by size: 973 260 129 36 BglI GCCn_nnn'nGGC Cuts at: 0 308 1285 1398 Size: 308 977 113 Fragments arranged by size: 977 308 113 BglII A'GATC_T Cuts at: 0 1130 1398 Size: 1130 268 Bme1580I G_kGCm'C Cuts at: 0 798 1043 1290 1398 Size: 798 245 247 108 Fragments arranged by size: 798 247 245 108 BmgBI CAC'GTC Cuts at: 0 533 1398 Size: 533 865 BmrI ACTGGGnnnn_n' Cuts at: 0 663 1398 Size: 663 735 BmtI G_CTAG'C Cuts at: 0 463 1398 Size: 463 935 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 93 1034 1398 Size: 93 941 364 Fragments arranged by size: 941 364 93 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 43 1398 Size: 43 1355 Bpu10I CC'TnA_GC Cuts at: 0 973 1009 1138 1398 Size: 973 36 129 260 Fragments arranged by size: 973 260 129 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 495 1398 Size: 495 903 BsaI GGTCTCn'nnnn_ Cuts at: 0 136 332 563 1398 Size: 136 196 231 835 Fragments arranged by size: 835 231 196 136 BsaAI yAC'GTr Cuts at: 0 695 1398 Size: 695 703 BsaHI Gr'CG_yC Cuts at: 0 713 1100 1398 Size: 713 387 298 Fragments arranged by size: 713 387 298 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 24 1183 1213 1250 1280 1398 Size: 24 1159 30 37 30 118 Fragments arranged by size: 1159 118 37 30 30 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 769 772 1267 1398 Size: 769 3 495 131 Fragments arranged by size: 769 495 131 3 BseYI C'CCAG_C Cuts at: 0 113 209 788 857 1047 1398 Size: 113 96 579 69 190 351 Fragments arranged by size: 579 351 190 113 96 69 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 206 706 1216 1398 Size: 206 500 510 182 Fragments arranged by size: 510 500 206 182 BsiHKAI G_wGCw'C Cuts at: 0 740 1398 Size: 740 658 BsmI GAATG_Cn' Cuts at: 0 947 956 1398 Size: 947 9 442 Fragments arranged by size: 947 442 9 Bsp1286I G_dGCh'C Cuts at: 0 740 798 1043 1290 1398 Size: 740 58 245 247 108 Fragments arranged by size: 740 247 245 108 58 BspHI T'CATG_A Cuts at: 0 911 1398 Size: 911 487 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 269 277 402 728 785 1398 Size: 269 8 125 326 57 613 Fragments arranged by size: 613 326 269 125 57 8 BsrBI CCG'CTC Cuts at: 0 405 1398 Size: 405 993 BsrDI GCAATG_nn' Cuts at: 0 495 1398 Size: 495 903 BsrFI r'CCGG_y Cuts at: 0 1004 1398 Size: 1004 394 BsrGI T'GTAC_A Cuts at: 0 419 1398 Size: 419 979 BssHII G'CGCG_C Cuts at: 0 1275 1398 Size: 1275 123 BssSI C'ACGA_G Cuts at: 0 739 1057 1398 Size: 739 318 341 Fragments arranged by size: 739 341 318 BstAPI GCAn_nnn'nTGC Cuts at: 0 27 134 229 1398 Size: 27 107 95 1169 Fragments arranged by size: 1169 107 95 27 BstYI r'GATC_y Cuts at: 0 1130 1205 1398 Size: 1130 75 193 Fragments arranged by size: 1130 193 75 BtgI C'CryG_G Cuts at: 0 155 1212 1329 1398 Size: 155 1057 117 69 Fragments arranged by size: 1057 155 117 69 BtsI GCAGTG_nn' Cuts at: 0 233 318 451 859 1224 1398 Size: 233 85 133 408 365 174 Fragments arranged by size: 408 365 233 174 133 85 DraIII CAC_nnn'GTG Cuts at: 0 279 352 854 1062 1398 Size: 279 73 502 208 336 Fragments arranged by size: 502 336 279 208 73 EaeI y'GGCC_r Cuts at: 0 318 554 1398 Size: 318 236 844 Fragments arranged by size: 844 318 236 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 82 289 1198 1398 Size: 82 207 909 200 Fragments arranged by size: 909 207 200 82 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 43 152 1398 Size: 43 109 1246 Fragments arranged by size: 1246 109 43 EcoNI CCTnn'n_nnAGG Cuts at: 0 1249 1398 Size: 1249 149 EcoO109I rG'GnC_Cy Cuts at: 0 188 612 633 794 795 1287 1317 1346 Size: 188 424 21 161 1 492 30 29 Cuts at: 1346 1357 1398 Size: 11 41 Fragments arranged by size: 492 424 188 161 41 30 29 21 11 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1140 1398 Size: 1140 258 HaeII r_GCGC'y Cuts at: 0 114 1103 1398 Size: 114 989 295 Fragments arranged by size: 989 295 114 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 26 1183 1215 1398 Size: 26 1157 32 183 Fragments arranged by size: 1157 183 32 26 KasI G'GCGC_C Cuts at: 0 1099 1398 Size: 1099 299 KpnI G_GTAC'C Cuts at: 0 126 261 837 1398 Size: 126 135 576 561 Fragments arranged by size: 576 561 135 126 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1181 1364 1398 Size: 1181 183 34 Fragments arranged by size: 1181 183 34 MscI TGG'CCA Cuts at: 0 320 1398 Size: 320 1078 MslI CAynn'nnrTG Cuts at: 0 275 910 1398 Size: 275 635 488 Fragments arranged by size: 635 488 275 MspA1I CmG'CkG Cuts at: 0 24 157 1331 1398 Size: 24 133 1174 67 Fragments arranged by size: 1174 133 67 24 NarI GG'CG_CC Cuts at: 0 1100 1398 Size: 1100 298 NheI G'CTAG_C Cuts at: 0 459 1398 Size: 459 939 PasI CC'CwG_GG Cuts at: 0 818 1354 1398 Size: 818 536 44 Fragments arranged by size: 818 536 44 PflMI CCAn_nnn'nTGG Cuts at: 0 279 887 1062 1360 1398 Size: 279 608 175 298 38 Fragments arranged by size: 608 298 279 175 38 PfoI T'CCnGG_A Cuts at: 0 1268 1398 Size: 1268 130 PmlI CAC'GTG Cuts at: 0 695 1398 Size: 695 703 PpuMI rG'GwC_Cy Cuts at: 0 188 1317 1346 1398 Size: 188 1129 29 52 Fragments arranged by size: 1129 188 52 29 PspOMI G'GGCC_C Cuts at: 0 794 1286 1398 Size: 794 492 112 Fragments arranged by size: 794 492 112 PstI C_TGCA'G Cuts at: 0 23 288 1398 Size: 23 265 1110 Fragments arranged by size: 1110 265 23 PvuII CAG'CTG Cuts at: 0 24 1398 Size: 24 1374 SacII CC_GC'GG Cuts at: 0 158 1332 1398 Size: 158 1174 66 Fragments arranged by size: 1174 158 66 SanDI GG'GwC_CC Cuts at: 0 1346 1398 Size: 1346 52 ScaI AGT'ACT Cuts at: 0 673 1398 Size: 673 725 SfcI C'TryA_G Cuts at: 0 19 284 1398 Size: 19 265 1114 Fragments arranged by size: 1114 265 19 SfoI GGC'GCC Cuts at: 0 1101 1398 Size: 1101 297 SmaI CCC'GGG Cuts at: 0 1096 1310 1398 Size: 1096 214 88 Fragments arranged by size: 1096 214 88 SmlI C'TyrA_G Cuts at: 0 474 1398 Size: 474 924 SrfI GCCC'GGGC Cuts at: 0 1310 1398 Size: 1310 88 StuI AGG'CCT Cuts at: 0 173 376 1398 Size: 173 203 1022 Fragments arranged by size: 1022 203 173 StyI C'CwwG_G Cuts at: 0 146 600 1398 Size: 146 454 798 Fragments arranged by size: 798 454 146 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 240 670 810 1039 1398 Size: 240 430 140 229 359 Fragments arranged by size: 430 359 240 229 140 TatI w'GTAC_w Cuts at: 0 419 671 1398 Size: 419 252 727 Fragments arranged by size: 727 419 252 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1032 1380 1398 Size: 1032 348 18 Fragments arranged by size: 1032 348 18 XmaI C'CCGG_G Cuts at: 0 1094 1308 1398 Size: 1094 214 90 Fragments arranged by size: 1094 214 90 Enzymes that do cut: AccI Acc65I AcuI AfeI AflIII AlwNI ApaI AseI AvaI BanI BanII BbeI BbvCI BglI BglII Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstYI BtgI BtsI DraIII EaeI EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NarI NheI PasI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacII SanDI ScaI SfcI SfoI SmaI SmlI SrfI StuI StyI TaqII TatI XcmI XmaI Enzymes that do not cut: AarI AatII AclI AflII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI BlpI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EarI EcoICRI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI MfeI MluI NaeI NcoI NdeI NgoMIV NotI NruI NsiI NspI PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SapI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SspI SwaI TstI Tth111I XbaI XhoI XmnI ZraI