################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AflII AgeI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspI AviII AvrII BamHI BanI BbeI BcgI BciVI BclI BfaI BfrI BlnI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CfoI Cfr10I ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MfeI MluI MroI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PflMI PinAI PmeI PpuMI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Tth111I Van91I XbaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 1507 GTCTAC GT^[AC][GT]AC AcyI 6 GACGCC G[GA]^CG[CT]C AflIII 110 ACATGT A^C[GA][CT]GT AhdI 51 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 1365 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 51 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 1475 TGGCCA TGG^CCA BbrPI 925 CACGTG CAC^GTG BbvCI 294 CCTCAGC CCTCAGC(-5/-2) BglII 1367 AGATCT A^GATCT BsaAI 925 CACGTG [CT]AC^GT[GA] BsaBI 1176 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 6 GACGCC G[GA]^CG[CT]C BsaWI 1415 ACCGGA [AT]^CCGG[AT] BsgI 159 GTGCAG GTGCAG(16/14) BsiEI 616 CGACCG CG[GA][CT]^CG BspLU11I 110 ACATGT A^CATGT BsrBI 1402 GAGCGG CCGCTC(-3/-3) BsrDI 185 GCAATG GCAATG(2/0) BstXI 11 CCAGGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 153 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 51 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 872 CCTTTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 776 CCTTGG C^C[AT][AT]GG EcoT22I 565 ATGCAT ATGCA^T Hin1I 6 GACGCC G[GA]^CG[CT]C MaeII 926 ACGT A^CGT MamI 1176 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1475 TGGCCA TGG^CCA MscI 1475 TGGCCA TGG^CCA MslI 1059 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NsiI 565 ATGCAT ATGCA^T PaeR7I 1446 CTCGAG C^TCGAG PmaCI 925 CACGTG CAC^GTG PmlI 925 CACGTG CAC^GTG PshAI 466 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 308 CTGCAG CTGCA^G StyI 776 CCTTGG C^C[AT][AT]GG TfiI 957 GAATC G^A[AT]TC XhoI 1446 CTCGAG C^TCGAG XmnI 1365 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AfaI 114 GTAC GT^AC 1284 GTAC AlwI 416 GATCC GGATC(4/5) 921 GATCC AocI 53 CCTCAGG CC^T[ACGT]AGG 715 CCTGAGG AspHI 1426 GTGCTC G[AT]GC[AT]^C 1587 GAGCAC BlpI 583 GCTGAGC GC^T[ACGT]AGC 1143 GCTGAGC Bpu1102I 583 GCTGAGC GC^T[ACGT]AGC 1143 GCTGAGC BsaI 768 GAGACC GGTCTC(1/5) 831 GGTCTC BseRI 37 CTCCTC GAGGAG(10/8) 1599 CTCCTC BsiHKAI 1426 GTGCTC G[AT]GC[AT]^C 1587 GAGCAC BstYI 920 AGATCC [GA]^GATC[CT] 1367 AGATCT Bsu36I 53 CCTCAGG CC^T[ACGT]AGG 715 CCTGAGG CelII 583 GCTGAGC GC^T[ACGT]AGC 1143 GCTGAGC EaeI 449 TGGCCG [CT]^GGCC[GA] 1475 TGGCCA EarI 484 CTCTTC CTCTTC(1/4) 1602 CTCTTC Eco81I 53 CCTCAGG CC^T[ACGT]AGG 715 CCTGAGG HgaI 6 GACGC GACGC(5/10) 249 GACGC Ksp632I 484 CTCTTC CTCTTC(1/4) 1602 CTCTTC MflI 920 AGATCC [GA]^GATC[CT] 1367 AGATCT MspA1I 519 CAGCGG C[AC]G^C[GT]G 1391 CAGCGG NspI 110 ACATGT [GA]CATG^[CT] 567 GCATGT PleI 178 GACTC GAGTC(4/5) 480 GACTC RsaI 114 GTAC GT^AC 1284 GTAC SfcI 308 CTGCAG C^T[GA][CT]AG 1388 CTACAG SmaI 887 CCCGGG CCC^GGG 1019 CCCGGG SmlI 1446 CTCGAG C^T[CT][GA]AG 1640 CTCAAG TaqI 414 TCGA T^CGA 1447 TCGA TthHB8I 414 TCGA T^CGA 1447 TCGA XcmI 816 CCAGGCGGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 836 CCATGATCTTCCTGG XhoII 920 AGATCC [GA]^GATC[CT] 1367 AGATCT XmaCI 887 CCCGGG C^CCGGG 1019 CCCGGG XmaI 887 CCCGGG C^CCGGG 1019 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AluI ApaI AvaI AvaII BanII BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I TspRI