################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AocI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvrII BamHI BbrPI BciVI BclI BfrI BlnI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspEI BspHI BspLU11I BsrGI BsrI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CpoI DraI DrdI Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI MamI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI ScaI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AfaI 140 GTAC GT^AC AflIII 1202 ACGTGT A^C[GA][CT]GT AhdI 961 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 382 GTGCAC G^TGCAC AlwI 1597 GATCC GGATC(4/5) ApaLI 382 GTGCAC G^TGCAC AspEI 961 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1659 TGCGCA TGC^GCA BfaI 1063 CTAG C^TAG BglII 1085 AGATCT A^GATCT BlpI 357 GCTGAGC GC^T[ACGT]AGC BpmI 1096 CTGGAG CTGGAG(16/14) Bpu1102I 357 GCTGAGC GC^T[ACGT]AGC BspDI 688 ATCGAT AT^CGAT BsrDI 362 GCAATG GCAATG(2/0) BssSI 733 CTCGTG CACGAG(-5/-1) CelII 357 GCTGAGC GC^T[ACGT]AGC ClaI 688 ATCGAT AT^CGAT DraIII 1276 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1367 CGGCCG C^GGCCG Eam1105I 961 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 583 CTCTTC CTCTTC(1/4) EclXI 1367 CGGCCG C^GGCCG Eco52I 1367 CGGCCG C^GGCCG FspI 1659 TGCGCA TGC^GCA HincII 346 GTCAAC GT[CT]^[GA]AC HindII 346 GTCAAC GT[CT]^[GA]AC Ksp632I 583 CTCTTC CTCTTC(1/4) KspI 1414 CCGCGG CCGC^GG MaeI 1063 CTAG C^TAG MaeII 1202 ACGT A^CGT NaeI 1672 GCCGGC GCC^GGC NgoMIV 1672 GCCGGC G^CCGGC PvuII 202 CAGCTG CAG^CTG RsaI 140 GTAC GT^AC SacII 1414 CCGCGG CCGC^GG SbfI 1158 CCTGCAGG CCTGCA^GG SfiI 945 GGCCCTCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 1158 CCTGCAGG CCTGCA^GG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Aor51HI 461 AGCGCT AGC^GCT 1022 AGCGCT AspHI 382 GTGCAC G[AT]GC[AT]^C 852 GTGCTC BbsI 253 GTCTTC GAAGAC(2/6) 748 GTCTTC BbvCI 87 CCTCAGC CCTCAGC(-5/-2) 818 CCTCAGC BcgI 248 GCAAGGTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 569 GCACAGCCATCG BpuAI 253 GTCTTC GAAGAC(2/6) 748 GTCTTC BsaI 979 GAGACC GGTCTC(1/5) 241 GGTCTC BseRI 681 CTCCTC GAGGAG(10/8) 964 GAGGAG BsiEI 900 CGACCG CG[GA][CT]^CG 1367 CGGCCG BsiHKAI 382 GTGCAC G[AT]GC[AT]^C 852 GTGCTC BsmAI 979 GAGAC GTCTC(1/5) 242 GTCTC BsrFI 353 ACCGGC [GA]^CCGG[CT] 1672 GCCGGC BstYI 1085 AGATCT [GA]^GATC[CT] 1596 AGATCC Cfr10I 353 ACCGGC [GA]^CCGG[CT] 1672 GCCGGC Eco47III 461 AGCGCT AGC^GCT 1022 AGCGCT MaeIII 301 GTCAC ^GT[ACGT]AC 736 GTGAC MflI 1085 AGATCT [GA]^GATC[CT] 1596 AGATCC NcoI 474 CCATGG C^CATGG 554 CCATGG NspI 158 ACATGC [GA]CATG^[CT] 323 GCATGT PflMI 71 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 588 CCAGACGCTGG PpuMI 1163 AGGTCCC [GA]G^G[AT]CC[CT] 1256 AGGACCT PstI 718 CTGCAG CTGCA^G 1159 CTGCAG SexAI 1010 ACCTGGT A^CC[AT]GGT 1277 ACCTGGT SfcI 718 CTGCAG C^T[GA][CT]AG 1159 CTGCAG Tsp45I 301 GTCAC ^GT[GC]AC 736 GTGAC Van91I 71 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 588 CCAGACGCTGG XcmI 185 CCACCTACTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 644 CCACCATCGTGGTGG XhoII 1085 AGATCT [GA]^GATC[CT] 1596 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AccII AciI AcyI AluI AlwNI ApaI AvaI AvaII BalI BanI BanII BbeI BbvI BcnI BglI BmyI BsaHI BsaJI BsgI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrBI BssHII BssKI BstAPI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SfoI SmaI StyI TaqI TseI TspRI TthHB8I XmaCI XmaI