(Circular) (Six-base) MAPSORT of: pFN21AE9431.seq Check: 7202 from: 1 to: 6532 pFN21AE9431 6532 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2599 2599 Size: 6532 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4342 278 Size: 53 83 186 936 2806 2468 Fragments arranged by size: 2806 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3875 1937 Size: 1938 4594 Acc65I G'GTAC_C Cuts at: 3853 3853 Size: 6532 AclI AA'CG_TT Cuts at: 1563 4659 5032 1563 Size: 3096 373 3063 Fragments arranged by size: 3096 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2785 4595 5979 6247 1632 Size: 1153 1810 1384 268 1917 Fragments arranged by size: 1917 1810 1384 1153 268 AfeI AGC'GCT Cuts at: 2468 3029 2468 Size: 561 5971 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6335 AflIII A'CryG_T Cuts at: 1203 3207 5431 6408 1203 Size: 2004 2224 977 1327 Fragments arranged by size: 2224 2004 1327 977 AgeI A'CCGG_T Cuts at: 5363 5363 Size: 6532 AhdI GACnn_n'nnGTC Cuts at: 2971 5261 2971 Size: 2290 4242 AleI CACnn'nnGTG Cuts at: 1166 2654 1166 Size: 1488 5044 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6532 AlwNI CAG_nnn'CTG Cuts at: 2599 2875 3547 4275 5847 2599 Size: 276 672 728 1572 3284 Fragments arranged by size: 3284 1572 728 672 276 ApaI G_GGCC'C Cuts at: 3303 3471 3567 3303 Size: 168 96 6268 Fragments arranged by size: 6268 168 96 ApaLI G'TGCA_C Cuts at: 2387 4589 5745 2387 Size: 2202 1156 3174 Fragments arranged by size: 3174 2202 1156 ApoI r'AATT_y Cuts at: 1493 3841 4067 4219 6328 1493 Size: 2348 226 152 2109 1697 Fragments arranged by size: 2348 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5086 160 Size: 872 4054 1606 Fragments arranged by size: 4054 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6532 AvaI C'yCGr_G Cuts at: 1870 1943 3302 3464 3560 3719 3787 3857 1870 Size: 73 1359 162 96 159 68 70 4545 Fragments arranged by size: 4545 1359 162 159 96 73 70 68 BamHI G'GATC_C Cuts at: 3862 4229 3862 Size: 367 6165 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2102 2616 3309 3853 5308 Size: 325 217 627 315 514 693 544 1455 Cuts at: 5308 618 Size: 1842 Fragments arranged by size: 1842 1455 693 627 544 514 325 315 217 BanII G_rGCy'C Cuts at: 1391 2709 3254 3303 3430 3471 3567 3647 3720 Size: 1318 545 49 127 41 96 80 73 Cuts at: 3720 3824 3851 1391 Size: 104 27 4072 Fragments arranged by size: 4072 1318 545 127 104 96 80 73 49 41 27 BbeI G_GCGC'C Cuts at: 2106 2620 3313 2106 Size: 514 693 5325 Fragments arranged by size: 5325 693 514 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2251 2746 928 Size: 971 352 495 4714 Fragments arranged by size: 4714 971 495 352 BbvCI CC'TCA_GC Cuts at: 2093 2824 2093 Size: 731 5801 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2242 2276 2563 2597 4723 4757 1903 Size: 34 305 34 287 34 2126 34 3678 Fragments arranged by size: 3678 2126 305 287 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4424 5634 4424 Size: 1210 5322 BclI T'GATC_A Cuts at: 1548 1548 Size: 6532 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2775 2957 3181 Size: 107 122 71 631 605 1103 182 224 Cuts at: 3181 3275 3298 3307 3724 5143 136 Size: 94 23 9 417 1419 1525 Fragments arranged by size: 1525 1419 1103 631 605 417 224 182 122 107 94 71 23 9 BglII A'GATC_T Cuts at: 3090 6527 3090 Size: 3437 3095 BlpI GC'TnA_GC Cuts at: 2363 3949 2363 Size: 1586 4946 Bme1580I G_kGCm'C Cuts at: 1790 2184 2391 2649 3303 3471 3567 4593 5749 Size: 394 207 258 654 168 96 1026 1156 Cuts at: 5749 1790 Size: 2573 Fragments arranged by size: 2573 1156 1026 654 394 258 207 168 96 BmgBI CAC'GTC Cuts at: 1318 3208 1318 Size: 1890 4642 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4263 5221 442 Size: 416 53 83 399 24 2846 958 1753 Fragments arranged by size: 2846 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6532 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3122 5192 1239 Size: 1883 2070 2579 Fragments arranged by size: 2579 2070 1883 Bpu10I CC'TnA_GC Cuts at: 2093 2824 3698 2093 Size: 731 874 4927 Fragments arranged by size: 4927 874 731 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4657 5522 5820 4657 Size: 865 298 5369 Fragments arranged by size: 5369 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2252 2978 5195 882 Size: 617 753 726 2217 2219 Fragments arranged by size: 2219 2217 753 726 617 BsaAI yAC'GTr Cuts at: 493 1204 4323 493 Size: 711 3119 2702 Fragments arranged by size: 3119 2702 711 BsaBI GATnn'nnATC Cuts at: 6526 6526 Size: 6532 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2103 2523 2617 2820 Size: 53 83 186 936 570 420 94 203 Cuts at: 2820 3310 3507 3620 4339 4721 5356 275 Size: 490 197 113 719 382 635 1451 Fragments arranged by size: 1451 936 719 635 570 490 420 382 203 197 186 113 94 83 53 BsaWI w'CCGG_w Cuts at: 4965 5363 5637 5784 6195 4965 Size: 398 274 147 411 5302 Fragments arranged by size: 5302 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6307 Fragments arranged by size: 6307 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2677 2984 1186 Size: 1491 307 4734 Fragments arranged by size: 4734 1491 307 BseYI C'CCAG_C Cuts at: 3117 3246 3636 3817 5735 6231 3117 Size: 129 390 181 1918 496 3418 Fragments arranged by size: 3418 1918 496 390 181 129 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2055 2225 2557 2627 3788 1163 Size: 573 319 170 332 70 1161 3907 Fragments arranged by size: 3907 1161 573 332 319 170 70 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2908 3375 3982 4743 5771 1540 Size: 275 186 907 467 607 761 1028 2301 Fragments arranged by size: 2301 1028 907 761 607 467 275 186 BsiHKAI G_wGCw'C Cuts at: 2391 2861 4593 4678 5749 2391 Size: 470 1732 85 1071 3174 Fragments arranged by size: 3174 1732 1071 470 85 BsmI GAATG_Cn' Cuts at: 4043 4136 4043 Size: 93 6439 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2184 2391 2649 2709 2861 3254 3303 Size: 399 394 207 258 60 152 393 49 Cuts at: 3303 3430 3471 3567 3647 3720 3824 3851 4593 Size: 127 41 96 80 73 104 27 742 Cuts at: 4593 4678 5749 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 394 393 258 207 152 127 104 96 85 80 73 60 49 41 27 BspEI T'CCGG_A Cuts at: 6195 6195 Size: 6532 BspHI T'CATG_A Cuts at: 4421 6151 4421 Size: 1730 4802 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2599 3157 3171 3887 844 Size: 1755 558 14 716 3489 Fragments arranged by size: 3489 1755 716 558 14 BsrBI CCG'CTC Cuts at: 1394 1823 2017 3307 3369 3480 4419 1394 Size: 429 194 1290 62 111 939 3507 Fragments arranged by size: 3507 1290 939 429 194 111 62 BsrDI GCAATG_nn' Cuts at: 65 1248 2374 5027 5201 65 Size: 1183 1126 2653 174 1396 Fragments arranged by size: 2653 1396 1183 1126 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2358 3677 5176 5363 1700 Size: 110 548 1319 1499 187 2869 Fragments arranged by size: 2869 1499 1319 548 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6532 BssHII G'CGCG_C Cuts at: 1925 3357 3359 3730 1925 Size: 1432 2 371 4727 Fragments arranged by size: 4727 1432 371 2 BssSI C'ACGA_G Cuts at: 2738 4592 5604 2738 Size: 1854 1012 3666 Fragments arranged by size: 3666 1854 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2386 3163 3286 794 Size: 1592 777 123 4040 Fragments arranged by size: 4040 1592 777 123 BstBI TT'CG_AA Cuts at: 5336 5336 Size: 6532 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6532 BstXI CCAn_nnnn'nTGG Cuts at: 2656 2659 3325 2656 Size: 3 666 5863 Fragments arranged by size: 5863 666 3 BstYI r'GATC_y Cuts at: 1972 3090 3601 3862 4229 4614 4631 6083 6169 Size: 1118 511 261 367 385 17 1452 86 Cuts at: 6169 6181 6527 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1118 511 385 367 346 261 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2199 2479 2559 2730 2862 3419 513 Size: 552 1134 280 80 171 132 557 3626 Fragments arranged by size: 3626 1134 557 552 280 171 132 80 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2170 2566 487 Size: 683 56 944 396 4453 Fragments arranged by size: 4453 944 683 396 56 BtsI GCAGTG_nn' Cuts at: 2038 2043 3128 4048 4842 4862 2038 Size: 5 1085 920 794 20 3708 Fragments arranged by size: 3708 1085 920 794 20 5 ClaI AT'CG_AT Cuts at: 1554 2694 1554 Size: 1140 5392 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6497 DraI TTT'AAA Cuts at: 3836 3836 Size: 6532 DraIII CAC_nnn'GTG Cuts at: 3286 3286 Size: 6532 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5539 809 Size: 507 4223 1802 Fragments arranged by size: 4223 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2154 2175 Size: 54 1425 50 275 18 243 81 21 Cuts at: 2175 2289 2556 2601 2958 3078 3267 3372 3979 Size: 114 267 45 357 120 189 105 607 Cuts at: 3979 4868 6497 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 607 357 275 267 243 189 120 114 105 81 54 50 45 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 3372 3979 1537 Size: 275 1560 607 4090 Fragments arranged by size: 4090 1560 607 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2594 4462 1353 Size: 1241 1868 3423 Fragments arranged by size: 3423 1868 1241 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3085 3472 5119 5493 5639 387 Size: 1064 138 1496 387 1647 374 146 1280 Fragments arranged by size: 1647 1496 1280 1064 387 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2785 3122 4595 5192 5979 6247 1239 Size: 393 1153 337 1473 597 787 268 1524 Fragments arranged by size: 1524 1473 1153 787 597 393 337 268 EcoO109I rG'GnC_Cy Cuts at: 2059 2872 3109 3169 3262 3544 3564 3613 2059 Size: 813 237 60 93 282 20 49 4978 Fragments arranged by size: 4978 813 282 237 93 60 49 20 EcoRI G'AATT_C Cuts at: 3841 4219 3841 Size: 378 6154 FspI TGC'GCA Cuts at: 3666 5038 3666 Size: 1372 5160 HaeII r_GCGC'y Cuts at: 1678 2106 2224 2470 2620 2686 2831 3031 3187 Size: 428 118 246 150 66 145 200 156 Cuts at: 3187 3313 3636 5679 1678 Size: 126 323 2043 2531 Fragments arranged by size: 2531 2043 428 323 246 200 156 150 145 126 118 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2957 2989 5173 5205 5247 Size: 32 135 32 1385 32 2184 32 42 Cuts at: 5247 5279 1373 Size: 32 2626 Fragments arranged by size: 2626 2184 1385 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2353 3876 4122 669 Size: 988 281 415 1523 246 3079 Fragments arranged by size: 3079 1523 988 415 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6532 HpaI GTT'AAC Cuts at: 4122 4122 Size: 6532 KasI G'GCGC_C Cuts at: 2102 2616 3309 2102 Size: 514 693 5325 Fragments arranged by size: 5325 693 514 KpnI G_GTAC'C Cuts at: 3857 3857 Size: 6532 MfeI C'AATT_G Cuts at: 4131 4131 Size: 6532 MluI A'CGCG_T Cuts at: 6408 6408 Size: 6532 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3003 5646 5830 1305 Size: 1698 2643 184 2007 Fragments arranged by size: 2643 2007 1698 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2075 2291 2558 2960 3080 Size: 54 1425 343 243 216 267 402 120 Cuts at: 3080 10 Size: 3462 Fragments arranged by size: 3462 1425 402 343 267 243 216 120 54 MslI CAynn'nnrTG Cuts at: 518 1166 2162 2333 2396 2564 2654 2657 3198 Size: 648 996 171 63 168 90 3 541 Cuts at: 3198 4490 4849 5008 518 Size: 1292 359 159 2042 Fragments arranged by size: 2042 1292 996 648 541 359 171 168 159 90 63 3 MspA1I CmG'CkG Cuts at: 1606 2209 3226 3421 3575 3640 3948 4625 5773 Size: 603 1017 195 154 65 308 677 1148 Cuts at: 5773 6018 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1017 677 603 308 245 195 154 65 NaeI GCC'GGC Cuts at: 3679 3679 Size: 6532 NarI GG'CG_CC Cuts at: 2103 2617 3310 2103 Size: 514 693 5325 Fragments arranged by size: 5325 693 514 NcoI C'CATG_G Cuts at: 513 1065 2479 2559 513 Size: 552 1414 80 4486 Fragments arranged by size: 4486 1414 552 80 NdeI CA'TA_TG Cuts at: 387 387 Size: 6532 NgoMIV G'CCGG_C Cuts at: 3677 3677 Size: 6532 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6532 NotI GC'GGCC_GC Cuts at: 3979 3979 Size: 6532 NspI r_CATG'y Cuts at: 1132 1229 2167 2332 3890 5435 1132 Size: 97 938 165 1558 1545 2229 Fragments arranged by size: 2229 1558 1545 938 165 97 PasI CC'CwG_GG Cuts at: 1348 3647 1348 Size: 2299 4233 PciI A'CATG_T Cuts at: 5431 5431 Size: 6532 PflMI CCAn_nnn'nTGG Cuts at: 1108 2082 2599 1108 Size: 974 517 5041 Fragments arranged by size: 5041 974 517 PfoI T'CCnGG_A Cuts at: 2934 3604 2934 Size: 670 5862 PmeI GTTT'AAAC Cuts at: 3836 3836 Size: 6532 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4601 4633 6114 6146 4601 Size: 32 1481 32 4987 Fragments arranged by size: 4987 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3169 3262 3169 Size: 93 6439 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6532 PsiI TTA'TAA Cuts at: 4102 4102 Size: 6532 PspOMI G'GGCC_C Cuts at: 3299 3467 3563 3299 Size: 168 96 6268 Fragments arranged by size: 6268 168 96 PstI C_TGCA'G Cuts at: 830 2727 3168 3884 830 Size: 1897 441 716 3478 Fragments arranged by size: 3478 1897 716 441 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6532 PvuII CAG'CTG Cuts at: 2209 2209 Size: 6532 SacII CC_GC'GG Cuts at: 3422 3422 Size: 6532 SalI G'TCGA_C Cuts at: 1936 3874 1936 Size: 1938 4594 SbfI CC_TGCA'GG Cuts at: 3168 3884 3168 Size: 716 5816 ScaI AGT'ACT Cuts at: 4780 6492 4780 Size: 1712 4820 SexAI A'CCwGG_T Cuts at: 3015 3282 3015 Size: 267 6265 SfcI C'TryA_G Cuts at: 826 1045 2723 3164 3880 5015 5696 5887 826 Size: 219 1678 441 716 1135 681 191 1471 Fragments arranged by size: 1678 1471 1135 716 681 441 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2957 2957 Size: 6532 SfoI GGC'GCC Cuts at: 2104 2618 3311 2104 Size: 514 693 5325 Fragments arranged by size: 5325 693 514 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6532 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6532 SmaI CCC'GGG Cuts at: 1872 3466 3562 3721 3859 1872 Size: 1594 96 159 138 4545 Fragments arranged by size: 4545 1594 159 138 96 SmlI C'TyrA_G Cuts at: 820 1017 1943 4636 5537 5799 820 Size: 197 926 2693 901 262 1553 Fragments arranged by size: 2693 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4323 493 Size: 3830 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6532 SphI G_CATG'C Cuts at: 1132 3890 1132 Size: 2758 3774 SrfI GCCC'GGGC Cuts at: 3562 3562 Size: 6532 SspI AAT'ATT Cuts at: 5 52 4456 5 Size: 47 4404 2081 Fragments arranged by size: 4404 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6532 StyI C'CwwG_G Cuts at: 513 1065 2127 2479 2559 3971 513 Size: 552 1062 352 80 1412 3074 Fragments arranged by size: 3074 1412 1062 552 352 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2174 2492 4559 4576 4729 4914 5359 Size: 1255 594 318 2067 17 153 185 445 Cuts at: 5359 325 Size: 1498 Fragments arranged by size: 2067 1498 1255 594 445 318 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4778 6490 96 Size: 274 80 33 51 1443 2801 1712 138 Fragments arranged by size: 2801 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6492 6524 6492 Size: 32 6500 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6532 XbaI T'CTAG_A Cuts at: 3868 3868 Size: 6532 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2197 2656 2197 Size: 459 6073 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6532 XmaI C'CCGG_G Cuts at: 1870 3464 3560 3719 3857 1870 Size: 1594 96 159 138 4545 Fragments arranged by size: 4545 1594 159 138 96 XmnI GAAnn'nnTTC Cuts at: 4661 4661 Size: 6532 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4340 276 Size: 53 83 186 936 2806 2468 Fragments arranged by size: 2806 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SwaI