(Linear) (Six-base) MAPSORT of: KIEE9431.seq Check: 9157 from: 1 to: 1827 KIEE9431.orf With 182 enzymes: SgfI * July 3, 2012 19:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 594 1827 Size: 594 1233 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 780 1827 Size: 780 1047 AfeI AGC'GCT Cuts at: 0 463 1024 1827 Size: 463 561 803 Fragments arranged by size: 803 561 463 AflIII A'CryG_T Cuts at: 0 1202 1827 Size: 1202 625 AhdI GACnn_n'nnGTC Cuts at: 0 966 1827 Size: 966 861 AleI CACnn'nnGTG Cuts at: 0 649 1827 Size: 649 1178 AlwNI CAG_nnn'CTG Cuts at: 0 594 870 1542 1827 Size: 594 276 672 285 Fragments arranged by size: 672 594 285 276 ApaI G_GGCC'C Cuts at: 0 1298 1466 1562 1827 Size: 1298 168 96 265 Fragments arranged by size: 1298 265 168 96 ApaLI G'TGCA_C Cuts at: 0 382 1827 Size: 382 1445 AvaI C'yCGr_G Cuts at: 0 1297 1459 1555 1714 1782 1827 Size: 1297 162 96 159 68 45 Fragments arranged by size: 1297 162 159 96 68 45 BanI G'GyrC_C Cuts at: 0 97 611 1304 1827 Size: 97 514 693 523 Fragments arranged by size: 693 523 514 97 BanII G_rGCy'C Cuts at: 0 704 1249 1298 1425 1466 1562 1642 1715 Size: 704 545 49 127 41 96 80 73 Cuts at: 1715 1819 1827 Size: 104 8 Fragments arranged by size: 704 545 127 104 96 80 73 49 41 8 BbeI G_GCGC'C Cuts at: 0 101 615 1308 1827 Size: 101 514 693 519 Fragments arranged by size: 693 519 514 101 BbsI GAAGACnn'nnnn_ Cuts at: 0 246 741 1827 Size: 246 495 1086 Fragments arranged by size: 1086 495 246 BbvCI CC'TCA_GC Cuts at: 0 88 819 1827 Size: 88 731 1008 Fragments arranged by size: 1008 731 88 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 237 271 558 592 1827 Size: 237 34 287 34 1235 Fragments arranged by size: 1235 287 237 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 770 952 1176 1270 1293 1302 1719 1827 Size: 770 182 224 94 23 9 417 108 Fragments arranged by size: 770 417 224 182 108 94 23 9 BglII A'GATC_T Cuts at: 0 1085 1827 Size: 1085 742 BlpI GC'TnA_GC Cuts at: 0 358 1827 Size: 358 1469 Bme1580I G_kGCm'C Cuts at: 0 179 386 644 1298 1466 1562 1827 Size: 179 207 258 654 168 96 265 Fragments arranged by size: 654 265 258 207 179 168 96 BmgBI CAC'GTC Cuts at: 0 1203 1827 Size: 1203 624 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1117 1827 Size: 1117 710 Bpu10I CC'TnA_GC Cuts at: 0 88 819 1693 1827 Size: 88 731 874 134 Fragments arranged by size: 874 731 134 88 BsaI GGTCTCn'nnnn_ Cuts at: 0 247 973 1827 Size: 247 726 854 Fragments arranged by size: 854 726 247 BsaHI Gr'CG_yC Cuts at: 0 98 518 612 815 1305 1502 1615 1827 Size: 98 420 94 203 490 197 113 212 Fragments arranged by size: 490 420 212 203 197 113 98 94 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 672 979 1827 Size: 672 307 848 Fragments arranged by size: 848 672 307 BseYI C'CCAG_C Cuts at: 0 1112 1241 1631 1812 1827 Size: 1112 129 390 181 15 Fragments arranged by size: 1112 390 181 129 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 50 220 552 622 1783 1827 Size: 50 170 332 70 1161 44 Fragments arranged by size: 1161 332 170 70 50 44 BsiEI CG_ry'CG Cuts at: 0 903 1370 1827 Size: 903 467 457 Fragments arranged by size: 903 467 457 BsiHKAI G_wGCw'C Cuts at: 0 386 856 1827 Size: 386 470 971 Fragments arranged by size: 971 470 386 Bsp1286I G_dGCh'C Cuts at: 0 179 386 644 704 856 1249 1298 1425 Size: 179 207 258 60 152 393 49 127 Cuts at: 1425 1466 1562 1642 1715 1819 1827 Size: 41 96 80 73 104 8 Fragments arranged by size: 393 258 207 179 152 127 104 96 80 73 60 49 41 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 594 1152 1166 1827 Size: 594 558 14 661 Fragments arranged by size: 661 594 558 14 BsrBI CCG'CTC Cuts at: 0 12 1302 1364 1475 1827 Size: 12 1290 62 111 352 Fragments arranged by size: 1290 352 111 62 12 BsrDI GCAATG_nn' Cuts at: 0 369 1827 Size: 369 1458 BsrFI r'CCGG_y Cuts at: 0 353 1672 1827 Size: 353 1319 155 Fragments arranged by size: 1319 353 155 BssHII G'CGCG_C Cuts at: 0 1352 1354 1725 1827 Size: 1352 2 371 102 Fragments arranged by size: 1352 371 102 2 BssSI C'ACGA_G Cuts at: 0 733 1827 Size: 733 1094 BstAPI GCAn_nnn'nTGC Cuts at: 0 381 1158 1281 1827 Size: 381 777 123 546 Fragments arranged by size: 777 546 381 123 BstXI CCAn_nnnn'nTGG Cuts at: 0 651 654 1320 1827 Size: 651 3 666 507 Fragments arranged by size: 666 651 507 3 BstYI r'GATC_y Cuts at: 0 1085 1596 1827 Size: 1085 511 231 Fragments arranged by size: 1085 511 231 BtgI C'CryG_G Cuts at: 0 194 474 554 725 857 1414 1827 Size: 194 280 80 171 132 557 413 Fragments arranged by size: 557 413 280 194 171 132 80 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 165 561 1827 Size: 165 396 1266 Fragments arranged by size: 1266 396 165 BtsI GCAGTG_nn' Cuts at: 0 33 38 1123 1827 Size: 33 5 1085 704 Fragments arranged by size: 1085 704 33 5 ClaI AT'CG_AT Cuts at: 0 689 1827 Size: 689 1138 DraIII CAC_nnn'GTG Cuts at: 0 1281 1827 Size: 1281 546 EaeI y'GGCC_r Cuts at: 0 68 149 170 284 551 596 953 1073 Size: 68 81 21 114 267 45 357 120 Cuts at: 1073 1262 1367 1827 Size: 189 105 460 Fragments arranged by size: 460 357 267 189 120 114 105 81 68 45 21 EagI C'GGCC_G Cuts at: 0 1367 1827 Size: 1367 460 EarI CTCTTCn'nnn_ Cuts at: 0 589 1827 Size: 589 1238 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1080 1467 1827 Size: 1080 387 360 Fragments arranged by size: 1080 387 360 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 780 1117 1827 Size: 780 337 710 Fragments arranged by size: 780 710 337 EcoO109I rG'GnC_Cy Cuts at: 0 54 867 1104 1164 1257 1539 1559 1608 Size: 54 813 237 60 93 282 20 49 Cuts at: 1608 1827 Size: 219 Fragments arranged by size: 813 282 237 219 93 60 54 49 20 FspI TGC'GCA Cuts at: 0 1661 1827 Size: 1661 166 HaeII r_GCGC'y Cuts at: 0 101 219 465 615 681 826 1026 1182 Size: 101 118 246 150 66 145 200 156 Cuts at: 1182 1308 1631 1827 Size: 126 323 196 Fragments arranged by size: 323 246 200 196 156 150 145 126 118 101 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 952 984 1827 Size: 952 32 843 Fragments arranged by size: 952 843 32 HincII GTy'rAC Cuts at: 0 348 1827 Size: 348 1479 KasI G'GCGC_C Cuts at: 0 97 611 1304 1827 Size: 97 514 693 523 Fragments arranged by size: 693 523 514 97 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 998 1827 Size: 998 829 MscI TGG'CCA Cuts at: 0 70 286 553 955 1075 1827 Size: 70 216 267 402 120 752 Fragments arranged by size: 752 402 267 216 120 70 MslI CAynn'nnrTG Cuts at: 0 157 328 391 559 649 652 1193 1827 Size: 157 171 63 168 90 3 541 634 Fragments arranged by size: 634 541 171 168 157 90 63 3 MspA1I CmG'CkG Cuts at: 0 204 1221 1416 1570 1635 1827 Size: 204 1017 195 154 65 192 Fragments arranged by size: 1017 204 195 192 154 65 NaeI GCC'GGC Cuts at: 0 1674 1827 Size: 1674 153 NarI GG'CG_CC Cuts at: 0 98 612 1305 1827 Size: 98 514 693 522 Fragments arranged by size: 693 522 514 98 NcoI C'CATG_G Cuts at: 0 474 554 1827 Size: 474 80 1273 Fragments arranged by size: 1273 474 80 NgoMIV G'CCGG_C Cuts at: 0 1672 1827 Size: 1672 155 NspI r_CATG'y Cuts at: 0 162 327 1827 Size: 162 165 1500 Fragments arranged by size: 1500 165 162 PasI CC'CwG_GG Cuts at: 0 1642 1827 Size: 1642 185 PflMI CCAn_nnn'nTGG Cuts at: 0 77 594 1827 Size: 77 517 1233 Fragments arranged by size: 1233 517 77 PfoI T'CCnGG_A Cuts at: 0 929 1599 1827 Size: 929 670 228 Fragments arranged by size: 929 670 228 PpuMI rG'GwC_Cy Cuts at: 0 1164 1257 1827 Size: 1164 93 570 Fragments arranged by size: 1164 570 93 PspOMI G'GGCC_C Cuts at: 0 1294 1462 1558 1827 Size: 1294 168 96 269 Fragments arranged by size: 1294 269 168 96 PstI C_TGCA'G Cuts at: 0 722 1163 1827 Size: 722 441 664 Fragments arranged by size: 722 664 441 PvuII CAG'CTG Cuts at: 0 204 1827 Size: 204 1623 SacII CC_GC'GG Cuts at: 0 1417 1827 Size: 1417 410 SbfI CC_TGCA'GG Cuts at: 0 1163 1827 Size: 1163 664 SexAI A'CCwGG_T Cuts at: 0 1010 1277 1827 Size: 1010 267 550 Fragments arranged by size: 1010 550 267 SfcI C'TryA_G Cuts at: 0 718 1159 1827 Size: 718 441 668 Fragments arranged by size: 718 668 441 SfiI GGCCn_nnn'nGGCC Cuts at: 0 952 1827 Size: 952 875 SfoI GGC'GCC Cuts at: 0 99 613 1306 1827 Size: 99 514 693 521 Fragments arranged by size: 693 521 514 99 SmaI CCC'GGG Cuts at: 0 1461 1557 1716 1827 Size: 1461 96 159 111 Fragments arranged by size: 1461 159 111 96 SrfI GCCC'GGGC Cuts at: 0 1557 1827 Size: 1557 270 StyI C'CwwG_G Cuts at: 0 122 474 554 1827 Size: 122 352 80 1273 Fragments arranged by size: 1273 352 122 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 169 487 1827 Size: 169 318 1340 Fragments arranged by size: 1340 318 169 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 192 651 1827 Size: 192 459 1176 Fragments arranged by size: 1176 459 192 XmaI C'CCGG_G Cuts at: 0 1459 1555 1714 1827 Size: 1459 96 159 113 Fragments arranged by size: 1459 159 113 96 Enzymes that do cut: AarI AcuI AfeI AflIII AhdI AleI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BpmI Bpu10I BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI ClaI DraIII EaeI EagI EarI EciI Eco57MI EcoO109I FspI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacII SbfI SexAI SfcI SfiI SfoI SmaI SrfI StyI TaqII XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BmrI BmtI BplI BpuEI BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrGI BstBI BstEII BstZ17I Bsu36I CspCI DraI DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI ScaI SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI